We narrowed to 21,843 results for: Tes

1. psiCHECK2-2A-3xFLAG-SBP-2A

   Plasmid

   #159584

   Purpose

   Luciferase reporter for ribosome pausing control

   Depositor

   Shintaro Iwasaki

   Article

   Han et al Cell Rep. 2020 May 5;31(5):107610. doi:

   Inserts

   2A-3xFLAG-SBP-2A
   EIF2S3 5UTR
   

   Use

   Luciferase

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Nov. 23, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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2. p3E-U6a-polg guide

   Plasmid

   #107598

   Purpose

   An entry vector with U6a promoter driving polg guide RNA expression

   Depositor

   Qing Deng

   Article

   Zhou et al Dis Model Mech. 2018 Mar 28;11(3). pii:

   Insert

   polg guide RNA sequence
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   Available since

   July 25, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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3. pCZGY847

   Plasmid

   #135093

   Purpose

   Transcriptional mcherry reporter of acr-2 (cholinergic) expressing neurons in C. elegans

   Depositor

   Yishi Jin

   Article

   Jospin et al PLoS Biol. 2009 Dec;7(12):e1000265. doi:

   Insert

   Pacr-2
   

   Use

   Tags

   mcherry and unc-10 3'UTR

   Expression

   Worm

   Mutation

   Promoter

   Pacr-2

   Available since

   Feb. 4, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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4. pGEX4T-1_WT_OCT4

   Plasmid

   #40633

   Depositor

   James Thomson

   Article

   Brumbaugh et al Proc Natl Acad Sci U S A. 2012 May 8;109

   Insert

   POU5F1 (POU5F1 Human)
   

   Use

   Tags

   GST, TEV cleavage, and Thrombin cleavage

   Expression

   Bacterial

   Mutation

   Promoter

   tac

   Available since

   July 26, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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5. pmiRFP720-tubulin

   Plasmid

   #136574

   Purpose

   Human alpha-tubulin labeling with a near-infrared fluorescent protein miRFP720

   Depositor

   Vladislav Verkhusha

   Article

   Matlashov et al Nat Commun. 2020 Jan 13;11(1):239. doi:

   Insert

   miRFP720-tubulin
   

   Use

   Tags

   miRFP720

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Feb. 11, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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6. hMTP

   Plasmid

   #124281

   Purpose

   Expresses human MTP in mammalian cells.

   Depositor

   Mahmood Hussain

   Article

   Sellers et al J Biol Chem. 2003 May 30;278(22):20367-7

   Insert

   MTTP (MTTP Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   June 3, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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7. Rogi2-CMV-LAMB3-T2A-GFP-bGH

   Plasmid

   #179865

   Purpose

   Targets Rogi2 genomic region with HDR and expresses LAMB3-T2A-GFP

   Depositor

   George Church

   Article

   Aznauryan et al Cell Rep Methods. 2022 Jan 14;2(1):10015

   Insert

   LAMB3-T2A-GFP
   

   Use

   Tags

   Expression

   Mutation

   Promoter

   Available since

   June 7, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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8. GFAP-Cre

   Plasmid

   #24704

   Depositor

   Michael Sofroniew

   Article

   Bush et al Neuron. 1999 Jun . 23(2):297-308.

   Insert

   Cre
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   GFAP (glial fibrillary acidic protein)

   Available since

   July 14, 2010

   Availability

   Academic Institutions and Nonprofits only

   

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9. pRG705_lexO-AsiSI_LexA-TF_LEU2MX

   Plasmid

   #154817

   Purpose

   Inducible expression of AsiSI endonuclease in S. cerevisiae

   Depositor

   Lorraine Symington

   Article

   Gnugge et al Nucleic Acids Res. 2020 Oct 14. pii: 592

   Inserts

   AsiSI endonuclease
   LexA-ER-B112
   

   Use

   Tags

   FLAG and NLS

   Expression

   Yeast

   Mutation

   Promoter

   ACT1 and lexO_4-CYC1_core

   Available since

   Oct. 26, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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10. pFRT_TO_FlagHA_TIA1

    Plasmid

    #106093

    Purpose

    gene expression of FLAGHA-TIA1

    Depositor

    Thomas Tuschl

    Article

    Meyer et al Mol Cell. 2018 Feb 15;69(4):622-635.e6.

    Insert

    TIA1 (TIA1 Human)
    

    Use

    Tags

    FlagHA

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Feb. 9, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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11. Nck

    Plasmid

    #186133

    Purpose

    For expression of recombinant Nck in bacteria cells.

    Depositor

    Michael Rosen

    Article

    Case et al Elife. 2022 Jan 20;11. pii: 72588. doi:

    Insert

    NCK1 (NCK1 Human)
    

    Use

    Tags

    GST

    Expression

    Bacterial

    Mutation

    Amino acid residues 1-377, with mutations: C139S,…

    Promoter

    Lac

    Available since

    June 30, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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12. BII-ChBtW-iresFUCCI

    Plasmid

    #133371

    Purpose

    Piggybac vector for constitutive expression

    Depositor

    Blair Madison

    Article

    Fischer et al Am J Physiol Gastrointest Liver Physiol.

    Insert

    Clover-Geminin-IRES-mKO2-Cdt1
    

    Use

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    N/A

    Promoter

    CMV enhancer and hEf1a (CpG free)

    Available since

    Jan. 3, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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13. pLG021 (AVAST type 5)

    Plasmid

    #157899

    Purpose

    Expresses the AVAST type 5 defense system

    Depositor

    Feng Zhang

    Article

    Gao et al Science. 2020 Aug 28;369(6507):1077-1084

    Insert

    AVAST type 5 (STAND ATPase with N-terminal SIR2)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Dec. 7, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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14. pGEX-5X1 Flag WDR5

    Plasmid

    #15553

    Depositor

    Kai Ge

    Article

    Cho et al J Biol Chem. 2007 Jul 13. 282(28):20395-

    Insert

    Wdr5 (Wdr5 Mouse)
    

    Use

    Tags

    Flag and GST

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Aug. 16, 2007

    Availability

    Academic Institutions and Nonprofits only

15. pUE1-TSP-BFLF1

    Plasmid

    #162657

    Purpose

    Expresses BFLF1 in mammalian cells with an N-terminal TwinStrep tag and HRV 3C protease cleavage site

    Depositor

    Britt Glaunsinger

    Article

    Didychuk et al Elife. 2021 Feb 8;10. pii: 62261. doi: 1

    Insert

    BFLF1
    

    Use

    Tags

    N-terminal 2xStrep-Tag II and HRV 3C site

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Feb. 17, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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16. HuEx-2xNLS-iGeoCas9(C1)-2xNLS_EGFP-g1

    Plasmid

    #222993

    Purpose

    Mammalian expression of iGeoCas9(C1) construct with EGFP-targeting sgRNA

    Depositor

    Jennifer Doudna

    Article

    Eggers et al Cell. 2024 May 13:S0092-8674(24)00457-4.

    Insert

    HuEx-2xNLS-iGeoCas9(C1)-2xNLS_EGFP-g1
    

    Use

    CRISPR

    Tags

    Puro

    Expression

    Mammalian

    Mutation

    Promoter

    pCAG

    Available since

    July 26, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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17. pCSC-NES-RFP

    Plasmid

    #221812

    Purpose

    Lentiviral vector expressing red fluorescent protein (RFP) fused with nuclear export sequence (NES)

    Depositor

    Baojin Ding

    Article

    Akter et al J Neurosci. 2024 Apr 10;44(15):e17282320

    Insert

    NES-mCherry
    

    Use

    Lentiviral

    Tags

    NES

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    July 2, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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18. pcDNA3-mCherry-TSC2

    Plasmid

    #192440

    Purpose

    Encodes mCherry-tagged wild-type TSC2 isoform 5.

    Depositor

    Jin Zhang

    Article

    Zhong et al Cell Chem Biol. 2022 Jun 16;29(6):1037-1

    Insert

    mCherry-TSC2 (TSC2 Human)
    

    Use

    Tags

    6xHIS - T7 tag (gene 10 leader) - Xpress (TM) tag…

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Dec. 7, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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19. pMSCV LDTS-eLIR

    Plasmid

    #189000

    Purpose

    lipophagy-inducing adaptor protein

    Depositor

    Atsushi Hoshino

    Article

    Minami et al Nat Commun. 2023 Jul 13;14(1):4084. doi:

    Insert

    LDTS-eLIR
    

    Use

    Retroviral

    Tags

    HA

    Expression

    Mutation

    Promoter

    Available since

    July 21, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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20. pLV-dfROX

    Plasmid

    #137170

    Purpose

    Lentiviral transfer vector for expression of roGFP2 and HyPer Red

    Depositor

    Shaun McCullough

    Article

    Faber et al Toxicol Sci. 2020 Jun 11. pii: 5856111.

    Insert

    roGFP2-2A-HyPer Red
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    EF1a

    Available since

    April 30, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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21. pENTR-NICD1

    Plasmid

    #46048

    Depositor

    Xin Chen

    Article

    Fan et al J Clin Invest. 2012 Aug 1;122(8):2911-5.

    Insert

    Notch1 (C-term) (Notch1 Mouse)
    

    Use

    Entry vector

    Tags

    Myc tag

    Expression

    Mutation

    Cytoplasmic domain initiating at amino acid 1753

    Promoter

    Available since

    July 2, 2013

    Availability

    Academic Institutions and Nonprofits only

22. pARF6-YFP

    Plasmid

    #11389

    Depositor

    Joel Swanson

    Article

    Beemiller et al PLoS Biol. 2006 May 9. 4(6):e162.

    Insert

    ADP Ribosylation Factor 6 (ARF6 Human)
    

    Use

    Tags

    YFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 3, 2006

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pHEE901

    Plasmid

    #91707

    Purpose

    CRISPR/Cas9-mediated base editing in Arabidopsis. Contains Cas9(D10A) and empty gRNA scaffold, HygR

    Depositor

    Qi-Jun Chen

    Article

    Chen et al Sci China Life Sci. 2017 Mar 16. doi: 10

    Type

    Empty backbone

    Use

    Tags

    Expression

    Plant

    Mutation

    Promoter

    Available since

    May 31, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pJH776_pJH_LldR_PlldR-sfGFP.3opal

    Plasmid

    #163735

    Purpose

    Lactate-inducible expression of green fluorescent protein encoding three opal codons

    Depositor

    Matthew Bennett

    Article

    Ho et al Nucleic Acids Res. 2021 Mar 18;49(5):e25

    Insert

    lldr gfp.3TGA
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    gfp with first three leucine codons mutated to am…

    Promoter

    Available since

    Sept. 24, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pcDNA3.1-HA-14-3-3 ϵ

    Plasmid

    #48797

    Purpose

    expresses human myc-14-3-3ϵ in mammalian cells

    Depositor

    Huda Zoghbi

    Article

    Chen et al Cell. 2003 May 16;113(4):457-68.

    Insert

    YWHAE 14-3-3 ϵ (YWHAE Human)
    

    Use

    Tags

    haemagglutinin

    Expression

    Mammalian

    Mutation

    Promoter

    cmv

    Available since

    June 4, 2014

    Availability

    Academic Institutions and Nonprofits only

26. pIRES-Puro-TCPTP

    Plasmid

    #33366

    Depositor

    Ambra Pozzi

    Article

    Borza et al J Biol Chem. 2010 Dec 17;285(51):40114-2

    Insert

    TCPTP-45 (PTPN2 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Dec. 8, 2011

    Availability

    Academic Institutions and Nonprofits only

27. px458_2A_GFP_sgRNA_TIAL1

    Plasmid

    #106096

    Purpose

    CRISPR knockout. Expresses Cas9, EGFP, and sgRNA targeting TIAL1

    Depositor

    Thomas Tuschl

    Article

    Meyer et al Mol Cell. 2018 Feb 15;69(4):622-635.e6.

    Insert

    gRNA TIAL1
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Feb. 9, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pSW068

    Plasmid

    #140036

    Purpose

    KO of PCC 11901 fadD gene/expresses codon-optimized 'tesA gene from E. coli from inducible cLac143 promoter

    Depositor

    Peter Nixon

    Article

    Wlodarczyk et al Commun Biol. 2020 May 7;3(1):215. doi: 1

    Insert

    Truncated thioesterase gene
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    cLac143

    Available since

    April 17, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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29. pKOR1-mcs

    Plasmid

    #181755

    Purpose

    Allele replacement for Staphylococcus aureus

    Depositor

    Suzan Rooijakkers

    Article

    Stapels et al Proc Natl Acad Sci U S A. 2014 Sep 9;111

    Type

    Empty backbone

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    April 14, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pLG017 (AVAST type 1)

    Plasmid

    #157895

    Purpose

    Expresses the AVAST type 1 defense system

    Depositor

    Feng Zhang

    Article

    Gao et al Science. 2020 Aug 28;369(6507):1077-1084

    Insert

    AVAST type 1 (STAND ATPase with N-terminal serine protease)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Dec. 7, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge