We narrowed to 14,348 results for: TIM
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Plasmid#181874PurposeExpresses the FRET-based mitochondrial calcium indicator 4mtD3cpv under control of the CaMKIIa promoterDepositorInsert4mtD3cpv
UseAAV and AdenoviralExpressionMammalianMutationthe 4mtD3cpv CDS differs from the recorded GeneBa…PromoterCaMKIIaAvailable SinceMarch 4, 2022AvailabilityAcademic Institutions and Nonprofits only -
pYZ145
Plasmid#98405PurposeDonor DNA plasmid to introduce leu1 deletion in S. pombe. Linearization with Not1 digestion.DepositorInsertsUseCRISPR and Synthetic BiologyAvailable SinceFeb. 5, 2018AvailabilityAcademic Institutions and Nonprofits only -
p35u4
Plasmid#174786PurposeConstitutive expression of λCI fused via three alanines to the MS2 coat protein. Useful as RNA-DNA adapter in three-hybrid systemDepositorInsertMS2 coat protein
TagsλCIExpressionBacterialMutationV30I, A81G, positions 68-80 deleted (VATQTVGGVELP…PromoterSynthetic Constitutive PromoterAvailable SinceMay 17, 2022AvailabilityAcademic Institutions and Nonprofits only -
pYZ149
Plasmid#98407PurposeDonor DNA plasmid to introduce his3 deletion in S. pombe. Linearization with Not1 digestion.DepositorInsertsUseCRISPR and Synthetic BiologyAvailable SinceFeb. 5, 2018AvailabilityAcademic Institutions and Nonprofits only -
pGEMHE:mTREK-1(tandem wt-wt)
Plasmid#224757PurposeIn vitro transcription for Oocyte expression of mTREK-1(tandem wt-wt)DepositorInsertTandem TREK-1 (wt-wt) (Kcnk2 Mouse)
UseIn vitro transcription for oocyte expressionPromoterT7Available SinceSept. 13, 2024AvailabilityAcademic Institutions and Nonprofits only -
pLdCH
Plasmid#84291PurposeExpress gRNA and Cas9 in Leishmania with Hygromycin resistanceDepositorInsertgRNA and Cas9
UseCRISPR; LeishmaniaPromoterL. donovani ribosome RNA promoterAvailable SinceJan. 30, 2017AvailabilityAcademic Institutions and Nonprofits only -
Intron-Tagging-pX330-Cas9-mCherry
Plasmid#159742PurposeExpresses Cas9-2A-mCherry and a sgRNA excising EGFP flanked by a splice acceptor and a splice donor from the Intron-Tagging-EGFP-Donor plasmid.DepositorInsertdonor plasmid targeting sgRNA
UseCRISPRExpressionMammalianPromoterU6Available SinceNov. 18, 2020AvailabilityAcademic Institutions and Nonprofits only -
hCCR5-LSSmOrange
Plasmid#110194PurposeEncodes for human CCR5 coding sequence tagged with the LSS-mOrange FPDepositorAvailable SinceMay 15, 2018AvailabilityAcademic Institutions and Nonprofits only -
pJH4203
Plasmid#200160PurposePrig-3 FRT let858 (stop) FRT twk-40(gf)::GFP unc-54 3' UTR C.elegans AVA and other neurons expression of twk-40 (gf ) GFPDepositorAvailable SinceMay 7, 2024AvailabilityIndustry, Academic Institutions, and Nonprofits -
pBAD-SPW-RHH
Plasmid#190630PurposeBacterial expression of genetically encoded protease indicator SPW-RHHDepositorInsertSPW-RHH
ExpressionBacterialAvailable SinceNov. 23, 2022AvailabilityAcademic Institutions and Nonprofits only -
pRB117.2
Plasmid#181949PurposeaTc-inducible expression of NarL with N-terminal mNeonGreen fusion. Also contains mCherry under NarL-controlled promoter PdcuSDepositorInsertTagsmNeonGreenExpressionBacterialMutationSee Depositor Comments BelowPromotermNG-NarL-PLtetO-1; mCherry-PdcuS; tetR-J23106Available SinceOct. 26, 2022AvailabilityAcademic Institutions and Nonprofits only -
HA-FOXO3a TM delta CT
Plasmid#1797DepositorInsertFOXO3a (FOXO3 Human)
TagsHAExpressionMammalianMutationT32A, S253A, S315A, delta CT (aa 468-673)Available SinceApril 20, 2006AvailabilityAcademic Institutions and Nonprofits only -
PB_EF1a-CasRx-msfGFP-2A-Blast
Plasmid#226010PurposeCasRx protein for RNA targeting in piggyBac backboneDepositorInsertCasRx RNA-targeting nuclease
UseCRISPRTagsmsfGFPExpressionMammalianAvailable SinceDec. 4, 2024AvailabilityAcademic Institutions and Nonprofits only -
pTE4254
Plasmid#89050PurposeExpresses St chimeric gRNA and human codon-optimized StCas9DepositorInsertsSt chimeric gRNA
hStCas9
UseCRISPRTagsSV40 NLSExpressionMammalianPromoterCMV and human U6Available SinceApril 14, 2017AvailabilityAcademic Institutions and Nonprofits only -
MMRN1-bio-His
Plasmid#53409PurposeExpresses full-length Multimerin-1 precursor ectodomain in mammalian cells. C-terminal rat Cd4d3+4 tag, biotinylation sequence and His tag.DepositorInsertMMRN1 (MMRN1 Human)
TagsHis tag, enzymatic biotinylation sequence, and ra…ExpressionMammalianPromoterCMVAvailable SinceFeb. 27, 2015AvailabilityAcademic Institutions and Nonprofits only -
pPHR-iRFP713
Plasmid#103818PurposeSoluble BLInCR mock effector that is recruited to 'localizer' sites upon blue light illuminationDepositorAvailable SinceMay 25, 2018AvailabilityAcademic Institutions and Nonprofits only -
pCMV-T7-AcrIIA17_Efa-NLS(SV40) (KAC91)
Plasmid#134333PurposeCMV and T7 promoter expression plasmid for human codon optimized AcrIIA17_Efa with C-terminal NLS (SV40)DepositorInserthuman codon optimized AcrIIA17_Efa
TagsNLS(SV40)ExpressionMammalianPromoterCMV and T7Available SinceMarch 30, 2020AvailabilityAcademic Institutions and Nonprofits only -
OmEF1aCas9P2ApuroSB
Plasmid#165484PurposeStable expression of a zebrafish optimized Cas9 driven by a tilapia promoter in fish cellsDepositorInsertZebrafish optimized Cas9 (nls-zcas9-nls from Wenbiao Chen Lab plasmid pCS2-nCas9n Addgene #47929)
UseCRISPR; TransposonPromoterOreochromis mossambicus EF1 alpha promoter (OmEF1…Available SinceMay 7, 2021AvailabilityAcademic Institutions and Nonprofits only -
pEB2-mCherry-L
Plasmid#104002PurposePlasmid encoding mCherry-LDepositorInsertmCherry-L
UseLow copyExpressionBacterialMutationMutations relative to DsRed (MSKGEE DNLA IIKEF… V…PromoterproCAvailable SinceDec. 27, 2017AvailabilityAcademic Institutions and Nonprofits only -
pCSN061
Plasmid#101725PurposeSingle copy yeast plasmid expressing Cas9 from Streptococcus pyogenes (SpCas9), codon optimized for expression in Saccharomyces cerevisiae.DepositorInsertsS. pyogenes Cas9 codon optimized for expression in S. cerevisiae. (NEWENTRY )
KanMX marker expression cassette.
UseCRISPRTagsSV40 NLSExpressionYeastPromoterHeterologous TEF1 promoter from A. gossypii. and …Available SinceDec. 8, 2017AvailabilityAcademic Institutions and Nonprofits only