We narrowed to 14,145 results for: TIM

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pET28a-MSP1D1deltaH4/2H5

Plasmid

#71715

Purpose

Membrane scaffold protein (MSP), helix 4/2 + helix5 deletion

Depositor

Gerhard Wagner

Article

Hagn et al J Am Chem Soc. 2013 Feb 6;135(5):1919-25

Insert

Apolipoprotein A1 (APOA1 Human)

Tags

6xHis and TEV site

Expression

Bacterial

Available Since

Dec. 15, 2015

Availability

Academic Institutions and Nonprofits only

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pMY-eGFP-P2A-PuroR-HA

Plasmid

#163343

Purpose

Retroviral vector to express cytosolic GFP and an HA-tagged puromycin selection marker

Depositor

Louis Ates, Teunis Geijtenbeek

Article

van der Donk et al Biol Open. 2022 Feb 15;11(2):bio059032.

Insert

GFP

Use

Retroviral

Expression

Mammalian

Promoter

LTR

Available Since

Jan. 14, 2021

Availability

Academic Institutions and Nonprofits only

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HA-FOXO3a T32A DBM (H212R)

Plasmid

#1793

Depositor

Michael Greenberg

Article

Tran et al Science 2002 Apr 19;296(5567):530-4.

Insert

FOXO3a (FOXO3 Human)

Tags

HA (YDVPDYASL)

Expression

Mammalian

Mutation

T32A, S253A, S315A, H212R

Available Since

April 20, 2006

Availability

Academic Institutions and Nonprofits only
pMY-MCS-P2A-Ly6G

Plasmid

#163353

Purpose

Retroviral backbone for in-frame cloning of a gene of interest and a P2A separated Ly6G surface marker

Depositor

Louis Ates, Teunis Geijtenbeek

Article

van der Donk et al Biol Open. 2022 Feb 15;11(2):bio059032.

Type

Empty backbone

Use

Retroviral

Expression

Mammalian

Available Since

Jan. 15, 2021

Availability

Academic Institutions and Nonprofits only

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pLentiguide-puro_hORAI1sg

Plasmid

#174326

Purpose

gRNA targeting hORAI1 - encodes puromycin resistance

Depositor

Sonal Srikanth

Article

Wu et al Cell Rep. 2021 Aug 24;36(8):109603. doi:

Insert

human Orai1 (ORAI1 Human)

Use

Lentiviral

Available Since

Dec. 8, 2021

Availability

Academic Institutions and Nonprofits only

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pAAV hSyn1 ChR2 ET-TC 2A tDimer

Plasmid

#101361

Purpose

High efficiency channelrhodopsin, neuron specific promoter, red fluorescent protein

Depositor

Thomas Oertner

Article

Berndt et al Proc Natl Acad Sci U S A. 2011 May 3;108

Inserts

Channelrhodopsin-2
synaptophysin-red fluorescent protein

Use

AAV

Expression

Mammalian

Mutation

E123T , T159C

Promoter

human Synapsin

Available Since

Feb. 5, 2018

Availability

Academic Institutions and Nonprofits only

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pBI-CMV1-mir144[909 nt]~451-CMV2-mir1

Plasmid

#164681

Purpose

Express miR-144[909 nt]~451 and miR-1-1 in mammalian cells

Depositor

David Bartel

Article

Fang et al Mol Cell. 2020 Apr 16;78(2):289-302.e6.

Inserts

miR-144[909 nt]~451 (MIR144 Human)
miR-1-1 (MIR1-1 Human)

Expression

Mammalian

Promoter

CMV

Available Since

March 25, 2021

Availability

Academic Institutions and Nonprofits only

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pCMV-T7-hAcrVA2.1-NLS(sv40) (BPK5059)

Plasmid

#115137

Purpose

CMV-T7 promoter expression plasmid for human codon optimized AcrVA2.1 anti-CRISPR protein with C-terminal NLS

Depositor

Joseph Bondy-Denomy, Keith Joung, Benjamin Kleinstiver

Article

Marino et al Science. 2018 Sep 6. pii: science.aau517

Insert

human codon optimized AcrVA2.1 anti-CRISPR protein

Tags

NLS(SV40)

Expression

Mammalian

Available Since

Sept. 11, 2018

Availability

Academic Institutions and Nonprofits only

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pQZ210

Plasmid

#106414

Purpose

Co-expresses trans SNARE complex with pDC183 in E.coli

Depositor

Axel Brunger

Article

Zhou et al Nature. 2017 Aug 24;548(7668):420-425. d

Inserts

Rat Synaptobrevin-2 fragment 28-66 (Vamp2 Synthetic)
Syntaxin-1A fragment 191-256 (Stx1a Rat, Synthetic)

Tags

His tag

Expression

Bacterial

Mutation

Fragment 191-256 , Codon optimization and Fragmen…

Promoter

T7

Available Since

Feb. 21, 2018

Availability

Academic Institutions and Nonprofits only

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pET28a-MSP1D1deltaH4/2

Plasmid

#71712

Purpose

Membrane scaffold protein (MSP), half of helix 4 truncated

Depositor

Gerhard Wagner

Article

Hagn et al J Am Chem Soc. 2013 Feb 6;135(5):1919-25

Insert

Apolipoprotein A1 (APOA1 Human)

Tags

6xHis and TEV site

Expression

Bacterial

Available Since

Dec. 15, 2015

Availability

Academic Institutions and Nonprofits only

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pU6-mir144 EF1Alpha-puro-T2A-BFP

Plasmid

#164791

Purpose

Express miR-144 under the U6 promoter in mammalian cells

Depositor

David Bartel

Article

Fang et al Mol Cell. 2020 Apr 16;78(2):289-302.e6.

Inserts

miR-144 (MIR144 Human)
puro-T2A-BFP

Use

Lentiviral

Expression

Mammalian

Promoter

EF1Alpha and U6

Available Since

March 15, 2021

Availability

Academic Institutions and Nonprofits only

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pROF402

Plasmid

#155334

Purpose

Binary vector for expression of FLuc controlled by the blue light-repressible system

Depositor

Matias Zurbriggen

Article

Ochoa-Fernandez et al Nat Methods. 2020 Jul;17(7):717-725. doi

Insert

LB_P35Senhancer(-953 to -51)-(C120)5-PhCMVmin-FLuc-T35S_RB

Use

Luciferase and Synthetic Biology; Eukaryotic expr…

Available Since

Oct. 26, 2023

Availability

Academic Institutions and Nonprofits only

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pROF441

Plasmid

#155336

Purpose

Binary vector for expression of a gRNA targeting AtAP1 promoter

Depositor

Matias Zurbriggen

Article

Ochoa-Fernandez et al Nat Methods. 2020 Jul;17(7):717-725. doi

Insert

LB_PAtU6-26-gRNA(PAtAP1)-sgRNA_RB

Use

CRISPR and Synthetic Biology

Expression

Plant

Available Since

Oct. 26, 2023

Availability

Academic Institutions and Nonprofits only

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pcDNA5 FRT TO myc Separase C2029A

Plasmid

#59822

Purpose

Allows the integration of myc Separase C2029A in the genome and Tet-inducible expression

Depositor

Stephen Taylor

Article

Holland et al J Cell Sci. 2006 Aug 15;119(Pt 16):3325-

Insert

Separase (ESPL1 Human)

Tags

Myc

Expression

Mammalian

Mutation

C2029A

Promoter

hybrid human cytomegalovirus (CMV)/TetO2 promoter

Available Since

May 12, 2015

Availability

Academic Institutions and Nonprofits only

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pRS313_ATP3

Plasmid

#120253

Purpose

Expression of ATP3 in yeast

Depositor

Markus Ralser

Article

Vowinckel et al Nat Metab. 2021 Nov;3(11):1521-1535. doi

Insert

ATP3 (ATP3 Budding Yeast)

Expression

Yeast

Promoter

ATP3

Available Since

April 8, 2021

Availability

Academic Institutions and Nonprofits only

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pET28a(DAP10)

Plasmid

#177904

Purpose

Bacterial expression of his10-tagged human DAP10 Intracellular Domain

Depositor

Ron Vale

Article

Hui et al Science. 2017 Mar 31;355(6332):1428-1433

Insert

DAP10 (aa 70-92 only) (HCST Human)

Tags

10xHis-Thrombin cut site-T7 tag

Expression

Bacterial

Promoter

T7

Available Since

May 7, 2024

Availability

Academic Institutions and Nonprofits only

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FLAG-β-arrestin2 RRK/Q

Plasmid

#38261

Depositor

Robert Lefkowitz

Article

Nelson et al J Biol Chem. 2008 Jul 25;283(30):21093-1

Insert

β-arrestin2 (Arrb2 Rat)

Tags

Flag

Expression

Mammalian

Mutation

Arg233, Arg237, and Lys251 converted to Glutamines

Promoter

CMV

Available Since

Aug. 22, 2012

Availability

Academic Institutions and Nonprofits only
pUC19.TRIM28.FKBP.knock-in.mCerulean

Plasmid

#185009

Purpose

For knocking in FKBP F36V P2A mCerulean

Depositor

Denes Hnisz

Article

Asimi et al Nat Genet. 2022 Aug;54(8):1238-1247. doi

Insert

FKBP F36V P2A mCerulean

Use

Cloning

Available Since

June 16, 2022

Availability

Academic Institutions and Nonprofits only

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pEGFP-C1-KSENB-Nup133-mut

Plasmid

#87339

Purpose

To express human Nup133 in mammalian cells. It contains synonymous mutations that are not targeted by shRNA

Depositor

Lei Lu

Article

Tie et al Biochem Biophys Res Commun. 2016 Sep 30;

Insert

NUP133 (NUP133 Human)

Tags

EGFP

Expression

Mammalian

Mutation

shRNA resistant changes; deletion of Nup133 AA1-7

Promoter

CMV

Available Since

May 4, 2017

Availability

Academic Institutions and Nonprofits only

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pBI-CMV1-mir451~144-CMV2-mir1

Plasmid

#164666

Purpose

Express miR-451~144 and miR-1-1 in mammalian cells

Depositor

David Bartel

Article

Fang et al Mol Cell. 2020 Apr 16;78(2):289-302.e6.

Inserts

miR-451~144 (MIR451A Human)
miR-1-1 (MIR1-1 Human)

Expression

Mammalian

Promoter

CMV

Available Since

March 29, 2021

Availability

Academic Institutions and Nonprofits only

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