We narrowed to 91,797 results for: lias

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pIRES-hrGFP II-TET1

Plasmid

#83568

Purpose

Expresses TET1 in mammalian cells

Depositor

Jean-Pierre Issa

Article

Jin et al Nucleic Acids Res. 2014 Jun;42(11):6956-

Insert

Tet Methylcytosine Dioxygenase 1 (TET1 Human)

Tags

3X FLAG tag and hr GFP II

Expression

Mammalian

Promoter

CMV

Available Since

Sept. 25, 2017

Availability

Academic Institutions and Nonprofits only

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STIM1-EGFP

Plasmid

#210370

Purpose

Expresses STIM1 fused with EGFP in mammalian cells

Depositor

Michele Pagano

Article

Li et al J Virol. 2023 Sep 28:e0050723. doi: 10.1

Insert

STIM1 (STIM1 Human)

Tags

EGFP

Expression

Mammalian

Available Since

Dec. 18, 2023

Availability

Academic Institutions and Nonprofits only

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NSP6-Flag

Plasmid

#210345

Purpose

Expresses SARS-CoV-2 NSP6 fused with Flag in mammalian cells

Depositor

Michele Pagano

Article

Li et al J Virol. 2023 Sep 28:e0050723. doi: 10.1

Insert

SARS-CoV-2 NSP6 (ORF1ab Human, SARS-CoV-2 isolate Wuhan-Hu-1)

Tags

Flag

Expression

Mammalian

Mutation

Many synonymous changes due to codon optimization

Available Since

Jan. 3, 2024

Availability

Academic Institutions and Nonprofits only

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pAS_4xMmaPylT(UUA)_EF1_MmaPylRS

Plasmid

#174896

Purpose

Mma PylRS expression for ochre suppression in mammalian cells; transient or piggy bac mediated integration

Depositor

Simon Elsaesser

Article

Meineke et al Cell Rep Methods. 2023 Nov 20;3(11):1006

Insert

Mma PylRS (pylS Methanosarcina mazei)

Expression

Mammalian

Promoter

EF1

Available Since

Nov. 7, 2023

Availability

Academic Institutions and Nonprofits only

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pLViP-LifeAct-iRFP670

Plasmid

#229699

Purpose

Lentiviral expression of LifeAct-iRFP670 in mammalian cells

Depositor

Alpha Yap

Article

Noordstra et al Dev Cell. 2023 Sep 25;58(18):1748-1763.e

Insert

Synthetic construct LifeAct-mTurquoise gene

Use

Lentiviral

Tags

iRFP670

Expression

Mammalian

Promoter

CMV promoter

Available Since

Jan. 16, 2025

Availability

Academic Institutions and Nonprofits only

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TMEM16F-M522K-peGFP-N1

Plasmid

#228579

Purpose

Expresses mouse TMEM16F-M522K-eGFP in mammalian cells

Depositor

Huanghe Yang

Article

Lowry et al bioRxiv [Preprint]. 2024 Sep 3:2024.02.0

Insert

TMEM16F/anoctamin 6 (Ano6), transcript variant 2 (Ano6 Mouse)

Expression

Mammalian

Mutation

M522K plus a 3 amino acid N-terminal truncation (…

Available Since

Dec. 2, 2024

Availability

Academic Institutions and Nonprofits only

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pEF5-FRT-TagRFP-T-IFT88

Plasmid

#61684

Purpose

Mammalian expression of IFT88 fused with N terminal TagRFP.T tag

Depositor

Maxence Nachury

Article

Ye et al Elife. 2013 Aug 6;2:e00654. doi: 10.7554

Insert

IFT88 (Ift88 Mouse)

Tags

TagRFP.T

Expression

Mammalian

Promoter

EF1a

Available Since

Jan. 6, 2015

Availability

Academic Institutions and Nonprofits only

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XLone-Puro Cas13d-eGFP U6 BbsI

Plasmid

#155184

Purpose

PiggybacTransposon-based tunable and temporal expression control of Cas13d- eGFP and U6 gRNA backbone

Depositor

Xiaoping Bao

Article

Chang et al Biomaterials. 2022 May 6;285:121569. doi

Type

Empty backbone

Use

Unspecified; Piggybac transposon

Expression

Mammalian

Promoter

U6

Available Since

June 24, 2021

Availability

Academic Institutions and Nonprofits only

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pCAG-EGFP-C-IFT81

Plasmid

#218732

Purpose

Expresses N-terminally EGFP-tagged IFT81 in mammalian cells

Depositor

Kazuhisa Nakayama

Article

Ishida et al Mol Biol Cell. 2022 Aug 1;33(9):ar83. do

Insert

IFT81 (IFT81 Human)

Tags

EGFP

Expression

Mammalian

Promoter

CAG

Available Since

May 22, 2024

Availability

Academic Institutions and Nonprofits only

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pMRX-IPU-GFP-MYH9

Plasmid

#168273

Purpose

Expresses MYH9 in mammalian cells

Depositor

Noboru Mizushima

Article

Yamamoto et al Nat Commun. 2021 Jun 2;12(1):3292. doi:

Insert

MYH9 (MYH9 Human)

Tags

FLAG

Expression

Mammalian

Available Since

June 8, 2021

Availability

Academic Institutions and Nonprofits only

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MO91-dCas9-AID(P182X)

Plasmid

#83260

Purpose

Expresses fusion of human codon-optimized S. pyogenes dCas9 (D10A, H840A ) protein with a human AID mutant protein (p182X). MSCV Retroviral backbone.

Depositor

Xing Chang

Article

Ma et al Nat Methods. 2016 Dec;13(12):1029-1035.

Insert

dCas9-AID P182X

Use

Retroviral

Tags

3xFLAG-NLS and FLAG

Expression

Mammalian

Mutation

D10A and H840A in Cas9; P182X in AID

Promoter

MSCV

Available Since

May 4, 2017

Availability

Academic Institutions and Nonprofits only
pHLmMBP-10

Plasmid

#72348

Purpose

Mammalian expression of secreted N-terminally 8His-tagged mMBP TEV cleavage site-linked fusion proteins with C-terminal 6His-tag/Strep-tag II/HA-tag

Depositor

Luca Jovine

Article

Bokhove et al J Struct Biol. 2016 Feb 3. 194:1-7. pii

Type

Empty backbone

Tags

6His-tag, 8His-tag, HA-tag, Strep-tag II, and mMB…

Expression

Mammalian

Promoter

CMV enhancer + chicken beta-actin promoter

Available Since

Feb. 17, 2016

Availability

Academic Institutions and Nonprofits only

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pLVX-Puro-TDP-43-Q331K

Plasmid

#133761

Purpose

Lentiviral expression of human TDP-43 Q331K disease mutant in mammalian cells.

Depositor

Shawn Ferguson

Article

Roczniak-Ferguson et al Life Sci Alliance. 2019 Sep 16;2(5). pii

Insert

TDP-43 (TARDBP Human)

Use

Lentiviral

Expression

Mammalian

Mutation

Changed glutamine 331 to lysine.

Promoter

CMV

Available Since

Dec. 2, 2019

Availability

Academic Institutions and Nonprofits only

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pSLQ2818 pPB: CAG-PYL1-KRAB-IRES-Puro-WPRE-SV40PA PGK-ABI-tagBFP-SpdCas9

Plasmid

#84241

Purpose

Expresses ABA-inducible KRAB-Sp dCas9

Depositor

Stanley Qi

Article

Gao et al Nat Methods. 2016 Oct 24. doi: 10.1038/n

Inserts

ABI-tagBFP-Sp dCas9
PYL1-KRAB

Use

CRISPR and Synthetic Biology; Piggybac

Tags

2xNLS (SV40), ABI, HA tag, IRES-Puro, PYL1, and t…

Expression

Mammalian

Promoter

CAG and PGK

Available Since

Nov. 9, 2016

Availability

Academic Institutions and Nonprofits only

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pcDNA3-Flag-RNF168

Plasmid

#133976

Purpose

mammalian expression vector of Flag tagged wild type RNF168

Depositor

Daniel Durocher

Article

Stewart et al Cell. 2009 Feb 6;136(3):420-34. doi: 10.

Insert

RNF168 (RNF168 Human)

Tags

Flag

Expression

Mammalian

Promoter

CMV

Available Since

March 4, 2020

Availability

Industry, Academic Institutions, and Nonprofits

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pHS2 (hANGPTL4-V5)

Plasmid

#102446

Purpose

Expresses V5-tagged human ANGPTL4 in mammalian cells

Depositor

Brandon Davies

Article

Chi et al J Biol Chem. 2015 May 8;290(19):11865-77

Insert

ANGPTL4 (ANGPTL4 Human)

Tags

V5

Expression

Mammalian

Promoter

CMV

Available Since

June 5, 2018

Availability

Academic Institutions and Nonprofits only

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pCAG-EGFP-C-IFT74

Plasmid

#218730

Purpose

Expresses N-terminally EGFP-tagged IFT74 in mammalian cells

Depositor

Kazuhisa Nakayama

Article

Nishijima et al Mol Biol Cell. 2017 Jun 15;28(12):1652-1

Insert

IFT74 (IFT74 Human)

Tags

EGFP

Expression

Mammalian

Promoter

CAG

Available Since

May 22, 2024

Availability

Academic Institutions and Nonprofits only

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pDisplay-GACh2.0

Plasmid

#106073

Purpose

express the genetically-encoded fluorescent ACh indicator GACh2.0 in mammalian cells

Depositor

Yulong Li

Article

Jing et al Nat Biotechnol. 2018 Sep;36(8):726-737.

Insert

GPCR activation based ACh sensor (GACh) 2.0

Expression

Mammalian

Promoter

CMV

Available Since

March 30, 2018

Availability

Academic Institutions and Nonprofits only

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GST-Rab8a-QL

Plasmid

#86079

Purpose

A bacterial expression plasmid encoding Rab8a GTPase-deficient mutant (Rab8a-Q67L) with N-terminus GST.

Depositor

Lei Lu

Article

Madugula et al J Cell Sci. 2016 Oct 15;129(20):3922-393

Insert

hRab8A (RAB8A Human)

Tags

GST

Expression

Bacterial

Mutation

Changed glutamine 67 to leucine

Promoter

tac

Available Since

May 9, 2017

Availability

Academic Institutions and Nonprofits only

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GST-Rab8a-TN

Plasmid

#86080

Purpose

A bacterial expression plasmid encoding GDP locked Rab8a (Rab8a-T22N) with N-terminus GST.

Depositor

Lei Lu

Article

Madugula et al J Cell Sci. 2016 Oct 15;129(20):3922-393

Insert

hRab8A (RAB8A Human)

Tags

GST

Expression

Bacterial

Mutation

Changed threonine 22 to aspargine

Promoter

tac

Available Since

May 9, 2017

Availability

Academic Institutions and Nonprofits only

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