We narrowed to 88,660 results for: Mal;

1. pDisplay-DNER

   Plasmid

   #51053

   Purpose

   expression of HA-DNER in mammalian cells

   Depositor

   Mineko Kengaku

   Article

   Eiraku et al J Biol Chem. 2002 Jul 12;277(28):25400-7

   Insert

   DNER (Dner Mouse)
   

   Tags

   HA and signal peptide

   Expression

   Mammalian

   Mutation

   without endogenous signal peptide (1-221bp)

   Promoter

   CMV

   Available Since

   Feb. 19, 2014

   Availability

   Academic Institutions and Nonprofits only

2. Circular 200,100 (mIDUA)

   Plasmid

   #170123

   Purpose

   AAV vector carrying 2 copies of a guide RNA targeting the mouse IDUA mRNA, expressed from human and mouse U6 promoters

   Depositor

   Prashant Mali

   Article

   Katrekar et al Nat Biotechnol. 2022 Feb 10. pii: 10.103

   Insert

   Circular 200,100 guide RNA (Idua Mouse)
   

   Use

   AAV

   Expression

   Mammalian

   Promoter

   Human U6 and mouse U6

   Available Since

   Feb. 11, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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3. Circular 200,100 (GAPDH)

   Plasmid

   #170120

   Purpose

   AAV vector carrying a guide RNA targeting the human GAPDH mRNA

   Depositor

   Prashant Mali

   Article

   Katrekar et al Nat Biotechnol. 2022 Feb 10. pii: 10.103

   Insert

   Circular 200,100 guide RNA (GAPDH Human)
   

   Use

   AAV

   Expression

   Mammalian

   Promoter

   Human U6

   Available Since

   Feb. 11, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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4. pCEP4-myc-PDGFRalpha-V242K

   Plasmid

   #136436

   Purpose

   Mammalian expression plasmid for c-myc-tagged PDGFRA (V242K mutant)

   Depositor

   Erik Procko

   Article

   Park et al PLoS Pathog. 2020 Jun 19;16(6):e1008647.

   Insert

   PDGFRalpha (PDGFRA Human)
   

   Tags

   Extracellular c-myc epitope tag and Signal peptid…

   Expression

   Mammalian

   Mutation

   V242K (disrupts PDGF binding, but still binds HCM…

   Promoter

   CMV

   Available Since

   July 9, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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5. pAG106 Lyn11-FAST

   Plasmid

   #130721

   Purpose

   Expresses FAST (also called YFAST) fused to Lyn11 sequence in mammalian cells

   Depositor

   Arnaud Gautier

   Article

   Plamont et al Proc Natl Acad Sci U S A. 2016 Jan 19;11

   Insert

   lyn11-FAST
   

   Tags

   myc-tag

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Sept. 13, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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6. LV_EF1a_CDK1(T14A,Y15F)-P2A-Hygro_Barcode

   Plasmid

   #170220

   Purpose

   Barcoded lentiviral vector to express CDK1 (T14A, Y15F) in mammalian cells under control of EF1a promoter. Barcoded for transcript detection in single cell RNA-seq

   Depositor

   Prashant Mali

   Article

   Parekh et al iScience. 2021 Sep 20;24(10):103149. doi

   Insert

   CDK1 (CDK1 Human)
   

   Use

   Lentiviral

   Mutation

   T14A, Y15F

   Promoter

   EF1a

   Available Since

   Sept. 23, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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7. pLEX-HA-birA*-K-Ras(G12D)-IRES-Puro

   Plasmid

   #120562

   Purpose

   Expresses HA-birA*-K-Ras G12D fusion protein in mammalian cells & for virus production

   Depositor

   Paul Khavari

   Article

   Kovalski et al Mol Cell. 2019 Jan 4. pii: S1097-2765(18

   Insert

   KRAS4B (KRAS Human)
   

   Use

   Lentiviral

   Tags

   HA-birA*

   Expression

   Mammalian

   Mutation

   G12D

   Promoter

   CMV

   Available Since

   March 18, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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8. pCAGGS-PHFF

   Plasmid

   #100280

   Purpose

   Expression vector for phycocyanobilin (PCB) synthesis in mammalian cells.

   Depositor

   Kazuhiro Aoki

   Article

   Uda et al Proc Natl Acad Sci U S A. 2017 Oct 24. p

   Insert

   MTS-PcyA-FLAG-P2A-MTS-HA-HO1-P2A-MTS-Myc-Fd-P2A-MTS-Fnr-T7 (synthetic genes)
   

   Tags

   FLAG, T7 and Mitochondria-targeting sequence (MTS…

   Expression

   Mammalian

   Mutation

   All genes are codon-optimized for expression in h…

   Promoter

   CAG promoter

   Available Since

   Sept. 20, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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9. EF1a_KLF4_P2A_Hygro_Barcode

   Plasmid

   #120455

   Purpose

   Barcoded lentiviral vector to express KLF4 in mammalian cells under control of EF1a promoter. Barcoded for transcript detection in single cell RNA-seq.

   Depositor

   Prashant Mali

   Article

   Parekh et al Cell Syst. 2018 Nov 12. pii: S2405-4712(

   Insert

   KLF4 (KLF4 Human)
   

   Use

   Lentiviral

   Promoter

   EF1a

   Available Since

   Feb. 5, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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10. pAG156 Mito-FAST

    Plasmid

    #130723

    Purpose

    Expresses FAST (also called YFAST) fused to a mitochondria targeting sequence (mito) in mammalian cells

    Depositor

    Arnaud Gautier

    Article

    Plamont et al Proc Natl Acad Sci U S A. 2016 Jan 19;11

    Insert

    Mito-FAST
    

    Tags

    Myc-tag

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Sept. 13, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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11. pcDNA3-sPDGFRalpha-Fc

    Plasmid

    #136438

    Purpose

    Mammalian expression plasmid for soluble ectodomain of PDGFR-alpha fused to IgG1 Fc

    Depositor

    Erik Procko

    Article

    Park et al PLoS Pathog. 2020 Jun 19;16(6):e1008647.

    Insert

    PDGFRalpha (PDGFRA Human)
    

    Tags

    Fc region of IgG1 (a.a. Asp-221 to Lys-447) and L…

    Expression

    Mammalian

    Mutation

    Extracellular domain of PDGFR-alpha (a.a. 1-524)

    Promoter

    CMV

    Available Since

    July 9, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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12. pCEP4-myc-S (Wuhan variant)

    Plasmid

    #169846

    Purpose

    Mammalian expression plasmid for S of SARS-CoV-2 (Wuhan variant) with N-terminal c-myc tag

    Depositor

    Erik Procko

    Article

    Zhang et al Nat Chem Biol. 2022 Mar;18(3):342-351. d

    Insert

    Protein S
    

    Tags

    HA leader and c-myc tag

    Expression

    Mammalian

    Mutation

    Codon-optimized for human cell expression.

    Promoter

    CMV

    Available Since

    Jan. 31, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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13. pAG109 H2B-FAST

    Plasmid

    #130722

    Purpose

    Expresses FAST (also called YFAST) fused to zebrafish histone H2B in mammalian cells

    Depositor

    Arnaud Gautier

    Article

    Plamont et al Proc Natl Acad Sci U S A. 2016 Jan 19;11

    Insert

    H2B-FAST
    

    Tags

    myc-tag

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Sept. 13, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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14. pTBL210

    Plasmid

    #126451

    Purpose

    Expresses Cas9-XTEN-TdT in mammalian cells.

    Depositor

    Chang Liu

    Article

    Loveless et al Nat Chem Biol. 2021 Jun;17(6):739-747. d

    Insert

    Cas9-XTEN-TdT (DNTT Human, Streptococcus pyogenes)
    

    Expression

    Mammalian

    Mutation

    See depositor comments below

    Promoter

    CMV

    Available Since

    June 4, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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15. dCas9-NLS-gs10-cLYN268

    Plasmid

    #188259

    Purpose

    Plasmid ensures constitutive expression of the C-terminal fragment of split LYN kinase domain (aa 239-267), fused to the catalytically inactive Cas9 (dCas9) protein, a flexible GS linker and the SV40 NLS signal at the N-terminus

    Depositor

    Roman Jerala

    Article

    Rihtar et al Nat Chem Biol. 2022 Sep 26. doi: 10.1038

    Insert

    cLYN268 (LYN Human)
    

    Use

    CRISPR

    Expression

    Mammalian

    Available Since

    Oct. 18, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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16. pINDUCER-21-LCOR

    Plasmid

    #97038

    Purpose

    Expresses LCOR in mammalian cells

    Depositor

    George Daley

    Article

    Sugimura et al Nature. 2017 May 17. doi: 10.1038/nature

    Insert

    Ligand Dependent Nuclear Receptor Corepressor (LCOR Human)
    

    Use

    Lentiviral

    Expression

    Mammalian

    Promoter

    Tet on

    Available Since

    Sept. 14, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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17. LV_EF1a_MEK1(S218D,S222D)-P2A-Hygro_Barcode

    Plasmid

    #170240

    Purpose

    Barcoded lentiviral vector to express MEK1 (S218D, S222D) in mammalian cells under control of EF1a promoter. Barcoded for transcript detection in single cell RNA-seq

    Depositor

    Prashant Mali

    Article

    Parekh et al iScience. 2021 Sep 20;24(10):103149. doi

    Insert

    MAP2K1 (MAP2K1 Human)
    

    Use

    Lentiviral

    Mutation

    S218D, S222D

    Promoter

    EF1a

    Available Since

    Sept. 23, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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18. EF1a_MYC Thr58Ala_P2A_Hygro_Barcode

    Plasmid

    #120513

    Purpose

    Barcoded lentiviral vector to express MYC Thr58Ala in mammalian cells under control of EF1a promoter. Barcoded for transcript detection in single cell RNA-seq.

    Depositor

    Prashant Mali

    Article

    Parekh et al Cell Syst. 2018 Nov 12. pii: S2405-4712(

    Insert

    MYC Thr58Ala (MYC Human)
    

    Use

    Lentiviral

    Mutation

    Point mutation changing Threonine to Alanine at a…

    Promoter

    EF1a

    Available Since

    Feb. 8, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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19. EF1a_MYC deltaNLS_P2A_Hygro_Barcode

    Plasmid

    #120506

    Purpose

    Barcoded lentiviral vector to express c-MYC deltaNLS in mammalian cells under control of EF1a promoter. Barcoded for transcript detection in single cell RNA-seq.

    Depositor

    Prashant Mali

    Article

    Parekh et al Cell Syst. 2018 Nov 12. pii: S2405-4712(

    Insert

    MYC H84NLS (MYC Human)
    

    Use

    Lentiviral

    Mutation

    Deletion of nuclear localization signal sequence

    Promoter

    EF1a

    Available Since

    Oct. 8, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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20. pcDNA3.1(-)-C2C1-ErNaR-TS-EYFP-ES

    Plasmid

    #209851

    Purpose

    Expression of human codon-optimized light-driven sodium pump ErNaR in mammalian cells

    Depositor

    Volker Busskamp

    Article

    Podoliak et al Nat Commun. 2024 Apr 10;15(1):3119. doi:

    Insert

    light-driven sodium pump ErNaR
    

    Tags

    EYFP

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    April 19, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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