20,362 results
-
Plasmid#122373PurposeExpresses mTagBFP2 in mammalian cellsDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertmTagBFP2
ExpressionMammalianAvailable sinceJune 30, 2020AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pET-21a_3xNLS_SpCas9_protein_expression
Plasmid#114365PurposeProtein expression plasmid for 3xNLS SpCas9 in pET-21a backboneDepositorInsert3xNLS_SpCas9
UseCRISPRTagsNLSExpressionBacterialPromoterT7Available sinceApril 18, 2019AvailabilityAcademic Institutions and Nonprofits onlyCited by -
VPS13A^mCherry
Plasmid#118758PurposeVPS13A internally tagged with mCherry at residue 1372.DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertVPS13A (VPS13A Human)
TagsmCherry inserted at position 1372ExpressionMammalianAvailable sinceNov. 29, 2018AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pHR-EF1Alpha-puro-T2A-Tet-on 3G-TRE3G-Ascl1
Plasmid#118593PurposeExpresses a puromycin resistance cassette with Tet-on 3G from the EF1Alpha promoter and Ascl1 from the TRE3G promoterDepositorInsertPuro-T2A-Tet-on 3G/Ascl1 (Ascl1 Mouse)
UseLentiviralExpressionMammalianPromoterEF1Alpha/TRE3GAvailable sinceNov. 29, 2018AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pAW62.YY1.FKBP.knock-in.mCherry
Plasmid#104370PurposeFor knocking in FKBP F36V P2A mCherry onto YY1DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertFKBP12(F36V) -P2A-mCherry
Available sinceDec. 21, 2017AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pMal-T-Avi-His/BirA
Plasmid#102962PurposeVector for in vivo biotinylation of a fusion protein in bacteria.DepositorTypeEmpty backboneExpressionBacterialAvailable sinceNov. 28, 2017AvailabilityAcademic Institutions and Nonprofits onlyCited by -
HaloTag-bActinCDS-bActinUTR-MS2V5
Plasmid#102718PurposeHalo-Actin reporter for detection of both mRNA and proteinDepositorInsertHaloTag-bActinCDS-bActin3'UTR-MS2V5
UseLentiviralTagsHaloTag and MS2V5PromoterUbCAvailable sinceNov. 16, 2017AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pSNAPf-H2B Control Plasmid
Plasmid#101124PurposeExpression of SNAP-tagged Histone H2B in mammalian cellsDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInserthistone cluster 1 H2B family member j (H2BC11 Human)
TagsSNAP-tag (SNAPf)ExpressionMammalianPromoterCMVAvailable sinceSept. 27, 2017AvailabilityIndustry, Academic Institutions, and NonprofitsCited by -
pX330a dCas9-LSD1
Plasmid#92362PurposeModified CAG promoter-containing vector for ubiquitous expression of catalytically inactive Cas9 fused to LSD1. For targeted enhancer demethylation in chicken embryos.DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertdCas9-LSD1
UseCRISPRExpressionMammalianPromoterCAGAvailable sinceAug. 24, 2017AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pEN396 - pCAGGS-Tir1-V5-2A-PuroR TIGRE donor
Plasmid#92142PurposeTargeting vector to introduce an osTir1 expressing cassette at the mouse TIGRE acceptor locus using Puromycin selection (2A-fusion). Auxin-inducible degron system.DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertosTir1
UseMouse TargetingTagsV5 tagExpressionMammalianAvailable sinceJune 26, 2017AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pEFIRES-P-ACSL3-mCherry
Plasmid#87158PurposeFluorescent protein targeted to lipid droplets (LDs) from the endoplasmic reticulum (ER)DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertACSL3 (ACSL3 Human)
TagsmCherryExpressionMammalianPromoterEF-1-alphaAvailable sinceMay 30, 2017AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pLenti Lifeact-EGFP BlastR
Plasmid#84383PurposeLentiviral expression of EGFP-tagged Lifeact with Blasticidin selection in cells including neuronsDepositorInsertLifeact
UseLentiviralTagsEGFPPromoterCMV immediate earlyAvailable sinceJan. 5, 2017AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pEGFP-C1-NuMA
Plasmid#81029PurposeExpresses human NuMA with N-terminal EGFP tag in mammalian cellsDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertHomo sapiens nuclear mitotic apparatus protein 1 (NUMA1) (NUMA1 Human)
TagsEGFPExpressionMammalianPromoterCMVAvailable sinceAug. 10, 2016AvailabilityAcademic Institutions and Nonprofits onlyCited by -
BPK3082
Plasmid#78742PurposeHuman expression plasmid for LbCpf1 guide RNA (need to clone in spacer into BsmBI sites)DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertLbCpf1 crRNA backbone, without spacer sequence
UseCRISPRExpressionMammalianPromoterU6Available sinceJune 16, 2016AvailabilityAcademic Institutions and Nonprofits onlyCited by -
Snap-Omp25
Plasmid#69599PurposeSnap-tag substrates with two different fluorophores (red and green) sequentially to separately label young and old mitochondriaDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertSnap-Omp25
ExpressionMammalianAvailable sinceDec. 16, 2015AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pSBtet-Bla
Plasmid#60510PurposeSB-transposon with inducible SfiI cloning site for GOI (contains firefly luciferase) and constitutive expression of rtTA and blasticidin resistance geneDepositorTypeEmpty backboneUseTransposonExpressionMammalianPromoterTCE & RPBSAAvailable sinceFeb. 11, 2015AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pCS2-Cre.zf1
Plasmid#61391PurposemRNA synthesis of Cre recombinase codon modified for zebrafishDepositorInsertCre.zf1
UseZebrafish expressionPromotersp6Available sinceJan. 28, 2015AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pICE-RNaseHI-D10R-E48R-NLS-mCherry
Plasmid#60367PurposePlasmid for expression of an inactive mutant of bacterial RNase HI tagged with NLS-mCherry that can be used to detect R-loops. Confers resistance to Puromycin. Expression is inducible in T-REx cells.DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertRNase HI-D10R-E48R
TagsNLS from SV40 T antigen and mCherryExpressionMammalianMutationD10R+E48R; catalyticaly inactivePromoterCMV-tetAvailable sinceJan. 8, 2015AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pLKO.DEST.EGFP
Plasmid#32684PurposeLentiviral cDNA/shRNA gateway destination vectorsDepositorTypeEmpty backboneUseLentiviralExpressionMammalianAvailable sinceJuly 1, 2014AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pDEST-hMeCP2-GFP
Plasmid#48078PurposeMammalian expression of GFP tagged MeCP2-WTDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertMethyl-CpG Binding Protein 2 (Rett Syndrome) (MECP2 Human)
TagsGFPExpressionMammalianMutationwild typePromoterCMVAvailable sinceApril 17, 2014AvailabilityAcademic Institutions and Nonprofits onlyCited by