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Showing: 601 - 630 of 13580 results

  1. pD649-HAsp-HAVCR2-Fc(DAPA)-AviTag-6xHis

    Plasmid
    #156584
    Purpose
    Mammalian expression of cell-surface protein extracellular domain fused to Fc(DAPA)-Avi-6xHis. Protein is secreted from cells.
    Depositor
    Chris Garcia
    Article
    Wojtowicz et al Cell. 2020 Aug 20;182(4):1027-1043.e17.
    Insert
    HAVCR2 (HAVCR2 Human)
    Use
    Tags
    Fc(DAPA)-AviTag-6xHis
    Expression
    Mammalian
    Mutation
    Fc region of human IgG contains D265A and P329A m…
    Promoter
    CMV/SP6
    Available since
    March 8, 2023
    Availability
    Academic Institutions and Nonprofits only
    156584-plasmid-map-sequence-id-426824

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  5. pCMV-T7-ABE8e-nSpRY-P2A-EGFP (KAC1069)

    Plasmid
    #185912
    Purpose
    pCMV and pT7 plasmid encoding human codon optimized ABE8e A-to-G base editor with nicakse SpRY(D10A/A61R/L1111R/D1135L/S1136W/G1218K/E1219Q/N1317R/A1322R/R1333P/R1335Q/T1337R) and P2A-EGFP
    Depositor
    Benjamin Kleinstiver
    Article
    Alves et al Nat Biomed Eng. 2023 Dec 6. doi: 10.1038
    Insert
    human codon optimized ABE8e-nSpRY-P2A-EGFP
    Use
    In vitro transcription; t7 promoter
    Tags
    BPNLS and BPNLS-3xFLAG-P2A-EGFP
    Expression
    Mammalian
    Mutation
    nSpRY=D10A/A61R/L1111R/D1135L/S1136W/G1218K/E1219…
    Promoter
    CMV and T7
    Available since
    July 20, 2022
    Availability
    Academic Institutions and Nonprofits only
    185912-plasmid-map-sequence-id-367362

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  6. pCMV-T7-ABE8.20m-nSpRY-P2A-EGFP (KAC1335)

    Plasmid
    #185917
    Purpose
    pCMV and pT7 plasmid encoding human codon optimized ABE8.20m A-to-G base editor with nicakse SpRY(D10A/A61R/L1111R/D1135L/S1136W/G1218K/E1219Q/N1317R/A1322R/R1333P/R1335Q/T1337R) and P2A-EGFP
    Depositor
    Benjamin Kleinstiver
    Article
    Alves et al Nat Biomed Eng. 2023 Dec 6. doi: 10.1038
    Insert
    human codon optimized ABE8.20m-nSpRY-P2A-EGFP
    Use
    In vitro transcription; t7 promoter
    Tags
    BPNLS and BPNLS-3xFLAG-P2A-EGFP
    Expression
    Mammalian
    Mutation
    nSpRY=D10A/A61R/L1111R/D1135L/S1136W/G1218K/E1219…
    Promoter
    CMV and T7
    Available since
    July 20, 2022
    Availability
    Academic Institutions and Nonprofits only
    185917-plasmid-map-sequence-id-367357

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  7. pCAG-GST-ATG9 (828-839)-ATG101 (1-198)

    Plasmid
    #188073
    Purpose
    To make ATG101/ATG13-HORMA and ATG9 C-terminal tail fusion complex for expression in mammalian cells
    Depositor
    James Hurley
    Article
    Ren et al Sci Adv. 2023 Feb 15;9(7):eadg2997. doi:
    Insert
    ATG9 and ATG101 (ATG9A, ATG101 Human)
    Use
    Tags
    GST tag
    Expression
    Mammalian
    Mutation
    codon-optimized ATG9 (828-839) and codon-optimize…
    Promoter
    CMV
    Available since
    Aug. 17, 2022
    Availability
    Academic Institutions and Nonprofits only
    188073-plasmid-map-sequence-id-369634

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  8. pDONR223_HAVCR2_WT_V5

    Plasmid
    #82965
    Purpose
    Gateway Donor vector containing HAVCR2, used as an over expression control for gene expression analysis. Part of the Target Accelerator Plasmid Collection.
    Depositor
    David Root, Jesse Boehm, Matthew Meyerson
    Article
    Berger et al Cancer Cell. 2016 Aug 8;30(2):214-28. do
    Insert
    HAVCR2 (HAVCR2 Human)
    Use
    Gateway entry vector
    Tags
    Expression
    Mutation
    R140L
    Promoter
    None
    Available since
    April 24, 2017
    Availability
    Academic Institutions and Nonprofits only
    82965-plasmid-map-sequence-id-162701

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  11. pCI-TPI_WT-IL6_3UTR-xrRNA-4H

    Plasmid
    #108373
    Purpose
    Expresses wild type TPI reporter; contains partial IL6 3' UTR; enables detection of 5'-3' decay intermediates (xrFrag) and allows detection via northern blot (4H binding sites)
    Depositor
    Niels Gehring
    Article
    Boehm et al Nat Commun. 2016 Dec 5;7:13691. doi: 10.
    Insert
    Wild type TPI reporter with partial IL6 control 3' UTR, MVE xrRNA and 4H probe binding sites (TPI1, IL6 Human)
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    May 1, 2018
    Availability
    Academic Institutions and Nonprofits only
    108373-plasmid-map-sequence-id-208350

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  12. pCI-TPI_WT-RAB7A_3UTR-xrRNA-4H

    Plasmid
    #108372
    Purpose
    Expresses wild type TPI reporter; contains partial RAB7A control 3' UTR; enables detection of 5'-3' decay intermediates (xrFrag) and allows detection via northern blot (4H binding sites)
    Depositor
    Niels Gehring
    Article
    Boehm et al Nat Commun. 2016 Dec 5;7:13691. doi: 10.
    Insert
    Wild type TPI reporter with partial RAB7A control 3' UTR, MVE xrRNA and 4H probe binding sites (TPI1, RAB7A Human)
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    May 3, 2018
    Availability
    Academic Institutions and Nonprofits only
    108372-plasmid-map-sequence-id-209438

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  13. pLenti-EML4-ALK variant 3a G1202R

    Plasmid
    #183830
    Purpose
    Express EML4-ALK fusion variant 3a (E6a;A20) harboring ALK G1202R mutation in mammalian cells
    Depositor
    Aaron Hata
    Article
    Yoda et al Cancer Discov. 2018 Jun;8(6):714-729. do
    Inserts
    ALK Receptor Tyrosine Kinase (ALK Human)
    EMAP Like 4 (EML4 Human)
    Use
    Lentiviral
    Tags
    The fusion protein of EML4 exon 1-6a and ALK exon…
    Expression
    Mammalian
    Mutation
    G1202R
    Promoter
    CMV
    Available since
    May 31, 2022
    Availability
    Academic Institutions and Nonprofits only
    183830-plasmid-map-sequence-id-360489

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  14. pLenti-EML4-ALK variant 3a L1196M/G1202R

    Plasmid
    #183831
    Purpose
    Express EML4-ALK fusion variant 3a (E6a;A20) harboring ALK L1196M/G1202R mutation in mammalian cells
    Depositor
    Aaron Hata
    Article
    Yoda et al Cancer Discov. 2018 Jun;8(6):714-729. do
    Inserts
    ALK Receptor Tyrosine Kinase (ALK Human)
    EMAP Like 4 (EML4 Human)
    Use
    Lentiviral
    Tags
    The fusion protein of EML4 exon 1-6a and ALK exon…
    Expression
    Mammalian
    Mutation
    L1196M/G1202R
    Promoter
    CMV
    Available since
    June 1, 2022
    Availability
    Academic Institutions and Nonprofits only
    183831-plasmid-map-sequence-id-360488

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  15. pUC57KAN-5XHA

    Plasmid
    #233102
    Purpose
    5X HA tag multimer
    Depositor
    Steve Stowers
    Insert
    HA tag
    Use
    Unspecified
    Tags
    Expression
    Mutation
    None
    Promoter
    Available since
    March 18, 2025
    Availability
    Academic Institutions and Nonprofits only
    233102-plasmid-map-sequence-id-465634

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  16. pUC57KAN-5XV5

    Plasmid
    #233105
    Purpose
    5X V5 tag multimer
    Depositor
    Steve Stowers
    Insert
    V5 tag
    Use
    Unspecified
    Tags
    Expression
    Mutation
    None
    Promoter
    Available since
    March 18, 2025
    Availability
    Academic Institutions and Nonprofits only
    233105-plasmid-map-sequence-id-465608

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  17. pUC57KAN-20XHA

    Plasmid
    #233114
    Purpose
    20X HA tag multimer
    Depositor
    Steve Stowers
    Insert
    HA tag
    Use
    Unspecified
    Tags
    Expression
    Mutation
    None
    Promoter
    Available since
    March 26, 2025
    Availability
    Academic Institutions and Nonprofits only
    233114-plasmid-map-sequence-id-466707

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  18. pUC57KAN-80XV5

    Plasmid
    #233128
    Purpose
    80X V5 tag multimer
    Depositor
    Steve Stowers
    Insert
    V5 tag
    Use
    Unspecified
    Tags
    Expression
    Mutation
    None
    Promoter
    Available since
    March 31, 2025
    Availability
    Academic Institutions and Nonprofits only
    233128-plasmid-map-sequence-id-467242

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  19. pUC57KAN-20XV5

    Plasmid
    #233117
    Purpose
    20X V5 tag multimer
    Depositor
    Steve Stowers
    Insert
    V5 tag
    Use
    Unspecified
    Tags
    Expression
    Mutation
    None
    Promoter
    Available since
    March 26, 2025
    Availability
    Academic Institutions and Nonprofits only
    233117-plasmid-map-sequence-id-466710

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  20. pUC57KAN-40XV5

    Plasmid
    #233123
    Purpose
    40X V5 tag multimer
    Depositor
    Steve Stowers
    Insert
    V5 tag
    Use
    Unspecified
    Tags
    Expression
    Mutation
    None
    Promoter
    Available since
    March 26, 2025
    Availability
    Academic Institutions and Nonprofits only
    233123-plasmid-map-sequence-id-466693

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  21. pUC57KAN-10XV5

    Plasmid
    #233111
    Purpose
    10X V5 tag multimer
    Depositor
    Steve Stowers
    Insert
    V5 tag
    Use
    Unspecified
    Tags
    Expression
    Mutation
    None
    Promoter
    Available since
    March 19, 2025
    Availability
    Academic Institutions and Nonprofits only
    233111-plasmid-map-sequence-id-465661

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  22. pUC57KAN-10XHA

    Plasmid
    #233108
    Purpose
    10X HA tag multimer
    Depositor
    Steve Stowers
    Insert
    HA tag
    Use
    Unspecified
    Tags
    Expression
    Mutation
    None
    Promoter
    Available since
    March 18, 2025
    Availability
    Academic Institutions and Nonprofits only
    233108-plasmid-map-sequence-id-465614

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  23. pUC57KAN-5XFLAG

    Plasmid
    #233101
    Purpose
    5X FLAG tag multimer
    Depositor
    Steve Stowers
    Insert
    FLAG tag
    Use
    Unspecified
    Tags
    Expression
    Mutation
    None
    Promoter
    Available since
    March 18, 2025
    Availability
    Academic Institutions and Nonprofits only
    233101-plasmid-map-sequence-id-465632

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  24. pUC57KAN-5XALFA

    Plasmid
    #233100
    Purpose
    5X ALFA tag multimer
    Depositor
    Steve Stowers
    Insert
    ALFA tag
    Use
    Unspecified
    Tags
    Expression
    Mutation
    None
    Promoter
    Available since
    March 18, 2025
    Availability
    Academic Institutions and Nonprofits only
    233100-plasmid-map-sequence-id-465631

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  25. pUC57KAN-5XMYC

    Plasmid
    #233103
    Purpose
    5X MYC tag multimer
    Depositor
    Steve Stowers
    Insert
    MYC tag
    Use
    Unspecified
    Tags
    Expression
    Mutation
    None
    Promoter
    Available since
    March 18, 2025
    Availability
    Academic Institutions and Nonprofits only
    233103-plasmid-map-sequence-id-465630

    Enlarge

  26. pUC57KAN-5XOLLAS

    Plasmid
    #233104
    Purpose
    5X OLLAS tag multimer
    Depositor
    Steve Stowers
    Insert
    OLLAS tag
    Use
    Unspecified
    Tags
    Expression
    Mutation
    None
    Promoter
    Available since
    March 18, 2025
    Availability
    Academic Institutions and Nonprofits only
    233104-plasmid-map-sequence-id-465629

    Enlarge

  27. pUC57KAN-40XALFA

    Plasmid
    #233118
    Purpose
    40X ALFA tag multimer
    Depositor
    Steve Stowers
    Insert
    ALFA tag
    Use
    Unspecified
    Tags
    Expression
    Mutation
    None
    Promoter
    Available since
    March 31, 2025
    Availability
    Academic Institutions and Nonprofits only
    233118-plasmid-map-sequence-id-467244

    Enlarge

  28. pUC57KAN-20XALFA

    Plasmid
    #233112
    Purpose
    20X ALFA tag multimer
    Depositor
    Steve Stowers
    Insert
    ALFA tag
    Use
    Unspecified
    Tags
    Expression
    Mutation
    None
    Promoter
    Available since
    March 26, 2025
    Availability
    Academic Institutions and Nonprofits only
    233112-plasmid-map-sequence-id-466717

    Enlarge

  29. pUC57KAN-80XFLAG

    Plasmid
    #233125
    Purpose
    78X FLAG tag multimer
    Depositor
    Steve Stowers
    Insert
    FLAG tag
    Use
    Unspecified
    Tags
    Expression
    Mutation
    None
    Promoter
    Available since
    April 1, 2025
    Availability
    Academic Institutions and Nonprofits only
    233125-plasmid-map-sequence-id-467240

    Enlarge

  30. pUC57KAN-40XFLAG

    Plasmid
    #233119
    Purpose
    39X FLAG tag multimer
    Depositor
    Steve Stowers
    Insert
    FLAG tag
    Use
    Unspecified
    Tags
    Expression
    Mutation
    None
    Promoter
    Available since
    April 1, 2025
    Availability
    Academic Institutions and Nonprofits only
    233119-plasmid-map-sequence-id-466695

    Enlarge

Showing: 601 - 630 of 13580 results