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We narrowed to 2,431 results for: pET28

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Showing: 1001 - 1050 of 2431 results

  8. pET28a-CohIII-CBM-HIS-ybbR

    Plasmid
    #60866
    Purpose
    Characteristic fingerprint molecule fused to cohesin type III domain to perform single molecule force spectroscopy experiments; covalently immobilized via introduced ybbR tag
    Depositor
    Hermann Gaub
    Article
    Schoeler et al Nat Commun. 2014 Dec 8;5:5635. doi: 10.1
    Inserts
    CohIII
    CBM
    Tags
    6xHIS, HRV 3C, and ybbR
    Expression
    Bacterial
    Mutation
    Cysteine 63 to Serine in CBM module
    Promoter
    T7
    Available Since
    Nov. 26, 2014
    Availability
    Academic Institutions and Nonprofits only
    60866-plasmid-map-sequence-id-98102

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  11. pET28a-HIS-Xyl-Xmod-DocIII

    Plasmid
    #60865
    Purpose
    Characteristic fingerprint molecule fused to dockerin type III domain to perform single molecule force spectroscopy experiments; covalently immobilized via introduced Cystein in Xylanase T120C
    Depositor
    Hermann Gaub
    Article
    Schoeler et al Nat Commun. 2014 Dec 8;5:5635. doi: 10.1
    Inserts
    Xyl
    Xmod-DocIII
    Tags
    6xHIS
    Expression
    Bacterial
    Mutation
    Threonine 120 to Cysteine in Xylanase domain
    Promoter
    T7
    Available Since
    Nov. 26, 2014
    Availability
    Academic Institutions and Nonprofits only
    60865-plasmid-map-sequence-id-98100

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  13. pET28a-Fgß-ddFLN4-HIS-ybbr

    Plasmid
    #101239
    Purpose
    wildtype Fgß peptide with ddFLN4 fingerprint domain with C-terminal ybbr tag for covalent immobilization - resulting in high forces
    Depositor
    Hermann Gaub
    Article
    Milles et al Science. 2018 Mar 30;359(6383):1527-1533
    Insert
    Fgß peptide, ddFLN4 fingerprint (FGB Human, D. discoideum)
    Tags
    HIS and ybbr
    Expression
    Bacterial
    Mutation
    C18S in ddFLN4
    Promoter
    T7
    Available Since
    March 29, 2018
    Availability
    Academic Institutions and Nonprofits only
    101239-plasmid-map-sequence-id-199088

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  15. pET28 E2 LBD-Tev-E1a-His

    Plasmid
    #232117
    Purpose
    For bacterial expression of a fusion protein containing from N to C-terminus: the lipoyl-binding domain of Human E2, a TEV protease site, a peptide from Human E1a, and a His tag.
    Depositor
    Ruth Sommese
    Article
    Liu et al J Biol Chem. 2023 Mar;299(3):102959. doi
    Insert
    DBT (DBT Human)
    Tags
    6xHis, RIGHHSTSDDSSAY (AA331 to 345 (preprocessin…
    Expression
    Bacterial
    Promoter
    T7 Promoter
    Available Since
    March 20, 2025
    Availability
    Academic Institutions and Nonprofits only
    232117-plasmid-map-sequence-id-465790

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  20. pET28a-ybbR-HIS-Xyl-DocI

    Plasmid
    #58711
    Purpose
    Characteristic fingerprint molecule to perform single molecule force spectroscopy experiments; covalently immobilized via ybbr tag
    Depositor
    Hermann Gaub
    Article
    Otten et al Nat Methods. 2014 Sep 7. doi: 10.1038/nm
    Insert
    Xyl-DocI
    Tags
    HIS, HRV 3C, and ybbR
    Expression
    Bacterial
    Mutation
    changed Threonin 120 to Cysteine in Xylanase Doma…
    Promoter
    T7
    Available Since
    Aug. 25, 2014
    Availability
    Academic Institutions and Nonprofits only
    58711-plasmid-map-sequence-id-90776

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  22. pET28a-RBP-GFP(S45C)-ZdK1

    Plasmid
    #185791
    Purpose
    Encodes green flourescent protein linked to RNA binding peptide (on N terminal end ) and Z domain fragment (variant 1) of Staphylococcal protein A (on C-terminal) via GS-linker
    Depositor
    Lizhong He
    Article
    Younas et al J Phys Chem Lett. 2020 Oct 15;11(20):864
    Insert
    RBP-sfGFP(S45C)-ZdK1
    Tags
    10xHis, TEV protease cleavage site after His Tag
    Expression
    Bacterial
    Mutation
    Ser to Cys mutation at start of GFP, residue 45 i…
    Promoter
    T7
    Available Since
    July 22, 2022
    Availability
    Academic Institutions and Nonprofits only
    185791-plasmid-map-sequence-id-367545

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  23. pET28a-ybbr-HIS-ddFLN4-K10

    Plasmid
    #101718
    Purpose
    K10 peptide with ddFLN4 fingerprint domain and N-terminal ybbr tag for covalent immobilization - resulting in high forces
    Depositor
    Hermann Gaub
    Article
    Milles et al Science. 2018 Mar 30;359(6383):1527-1533
    Insert
    ddFLN4 fingerprint, K10 peptide (KRT10 Human, Dictyostelium discoideum)
    Tags
    HIS and ybbr
    Expression
    Bacterial
    Mutation
    C18S in ddFLN4
    Promoter
    T7
    Available Since
    March 29, 2018
    Availability
    Academic Institutions and Nonprofits only
    101718-plasmid-map-sequence-id-196776

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  26. pET28a-ybbr-HIS-ddFLN4-Fgß

    Plasmid
    #101719
    Purpose
    wildtype Fgß peptide with ddFLN4 fingerprint domain with N-terminal ybbr tag for covalent immobilization - resulting in weak forces
    Depositor
    Hermann Gaub
    Article
    Milles et al Science. 2018 Mar 30;359(6383):1527-1533
    Insert
    ddFLN4 fingerprint, Fgß peptide (FGB Human, Dictyostelium discoideum)
    Tags
    HIS and ybbr
    Expression
    Bacterial
    Mutation
    C18S in ddFLN4
    Promoter
    T7
    Available Since
    March 29, 2018
    Availability
    Academic Institutions and Nonprofits only
    101719-plasmid-map-sequence-id-199092

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  27. pET28a SUMO Nup53xl 162-267

    Plasmid
    #86002
    Purpose
    bacterial expression of his6 and SUMO tagged RRM motif of Xenopus laevis Nup53
    Depositor
    Wolfram Antonin
    Type
    Empty backbone
    Tags
    SUMO
    Expression
    Bacterial
    Available Since
    April 11, 2017
    Availability
    Academic Institutions and Nonprofits only
    86002-plasmid-map-sequence-id-171435

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  28. pET28a-FgßF3-ddFLN4-HIS-ybbr

    Plasmid
    #101743
    Purpose
    mutant FgßF3 peptide (additional phenylalanine) with ddFLN4 fingerprint domain with C-terminal ybbr tag for covalent immobilization - resulting in high forces
    Depositor
    Hermann Gaub
    Article
    Milles et al Science. 2018 Mar 30;359(6383):1527-1533
    Insert
    FgßF3 peptide, ddFLN4 fingerprint (FGB Human, Dictyostelium discoideum)
    Tags
    HIS and ybbr
    Expression
    Bacterial
    Mutation
    C18S in ddFLN4, added 9F in FgßF3
    Promoter
    T7
    Available Since
    March 29, 2018
    Availability
    Academic Institutions and Nonprofits only
    101743-plasmid-map-sequence-id-199091

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  30. delNC C-His Ana GvpC in pET28a

    Plasmid
    #85736
    Purpose
    Expresses a truncated His-tagged GvpC from Anabaena flos-aquae without the N-and C-terminal regions in E.coli
    Depositor
    Mikhail Shapiro
    Article
    Lakshmanan et al ACS Nano. 2016 Aug 23;10(8):7314-22. doi
    Insert
    Gas vesicle protein C (truncated and his-tagged)
    Tags
    His-tag
    Expression
    Bacterial
    Mutation
    Deletion of N and C terminal regions, addition of…
    Promoter
    T7
    Available Since
    March 29, 2017
    Availability
    Academic Institutions and Nonprofits only
    85736-plasmid-map-sequence-id-174840

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  33. pET28b(+)_S. cerevisiae Hbs1(166-611)

    Plasmid
    #39338
    Depositor
    SeWon Suh
    Insert
    Elongation factor 1 alpha-like protein
    Tags
    His-tag
    Expression
    Bacterial
    Promoter
    T7
    Available Since
    Sept. 17, 2012
    Availability
    Academic Institutions and Nonprofits only

  36. pET28a(+)v2_TwinStrep-SUMO-FLP-8xHis

    Plasmid
    #246980
    Purpose
    Expresses E. coli codon-optimized TwinStrep-SUMO-tagged FLP recombinase with N- and C-terminal NLS, using an improved pET design from Shilling et al. 2020.
    Depositor
    Amro Hamdoun
    Insert
    FLP Recombinase
    Use
    Synthetic Biology
    Tags
    8xHis, Nucleoplasmin, SUMO, SV40, and TwinStrep
    Expression
    Bacterial
    Promoter
    T7
    Available Since
    Nov. 4, 2025
    Availability
    Academic Institutions and Nonprofits only
    246980-plasmid-map-sequence-id-491526

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  38. pET28b-SUMO-FLAG-TeKaiA-FLAG

    Plasmid
    #229220
    Purpose
    For expression of T. elongatus KaiA protein
    Depositor
    Andy LiWang
    Insert
    kaiA (kaiA T. elongatus)
    Tags
    FLAG and SUMO-FLAG
    Expression
    Bacterial
    Available Since
    Feb. 3, 2025
    Availability
    Academic Institutions and Nonprofits only
    229220-plasmid-map-sequence-id-460016

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  39. pET28b-SUMO-FLAG-TeKaiB-FLAG

    Plasmid
    #229221
    Purpose
    For expression of T. elongatus KaiB protein
    Depositor
    Andy LiWang
    Insert
    kaiB (kaiB T. elongatus)
    Tags
    FLAG and SUMO-FLAG
    Expression
    Bacterial
    Available Since
    Jan. 6, 2025
    Availability
    Academic Institutions and Nonprofits only
    229221-plasmid-map-sequence-id-457227

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  40. pET28b-SUMO-SeKaiB-K25C-FLAG

    Plasmid
    #229225
    Purpose
    For expression of S. elongatus KaiB protein mutant
    Depositor
    Andy LiWang
    Insert
    kaiB (kaiB S. elongatus)
    Tags
    FLAG and SUMO
    Expression
    Bacterial
    Mutation
    K25C
    Available Since
    Jan. 6, 2025
    Availability
    Academic Institutions and Nonprofits only
    229225-plasmid-map-sequence-id-457071

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  44. pET28a-SdrG-B2-ELP-HIS-ybbR

    Plasmid
    #169134
    Purpose
    E. coli expression of SdrG N2, N3 and B2 domains containing ybbR tag and ELP linker. This construct was designed for AFM-SMFS measurements.
    Depositor
    Michael Nash
    Article
    Liu et al JACS Au. 2022 May 18;2(6):1417-1427. doi
    Insert
    Staphylococcus epidermidis fibrinogen-binding protein SD-repeat protein G
    Tags
    3xELP linker, 6x Histag, and ybbr tag
    Expression
    Bacterial
    Available Since
    Oct. 10, 2024
    Availability
    Academic Institutions and Nonprofits only
    169134-plasmid-map-sequence-id-380322

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  47. pET28a-SdrG-B1-ELP-HIS-ybbR

    Plasmid
    #169133
    Purpose
    E. coli expression of SdrG N2, N3 and B1 domains containing ybbR tag and ELP linker. This construct was designed for AFM-SMFS measurements.
    Depositor
    Michael Nash
    Article
    Liu et al JACS Au. 2022 May 18;2(6):1417-1427. doi
    Insert
    Staphylococcus epidermidis fibrinogen-binding protein SD-repeat protein G
    Tags
    3xELP linker, 6x Histag, and ybbr tag
    Expression
    Bacterial
    Available Since
    Oct. 10, 2024
    Availability
    Academic Institutions and Nonprofits only
    169133-plasmid-map-sequence-id-361153

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  48. pET28_6xHis-GAL4-PARP1-CAT-L713F

    Plasmid
    #175949
    Purpose
    Bacterial expression of a fusion of yeast GAL4 DNA binding domain and human PARP1 CAT domain containing L713F gain-of-function mutation
    Depositor
    Joan Conaway
    Article
    Ooi et al Proc Natl Acad Sci U S A. 2021 Sep 7;118
    Insert
    GAL4 DNA binding domain (GAL4 Budding Yeast)
    Tags
    6xHis tag
    Expression
    Bacterial
    Mutation
    L713F, V762A for the 'PARP1-CAT-L713F' …
    Promoter
    T7
    Available Since
    July 19, 2024
    Availability
    Academic Institutions and Nonprofits only
    175949-plasmid-map-sequence-id-345352

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  49. pET28-H12TEV-hEIF5B-587~1220

    Plasmid
    #208637
    Purpose
    for E. coli expression truncated human eIF5B (ENST00000289371), aa587~1220
    Depositor
    Quanfu Ma
    Insert
    human eIF5B (ENST00000289371) aa 587~1220 (EIF5B Human)
    Tags
    6xhis
    Expression
    Bacterial
    Mutation
    contain SNP rs1376443, protein sequence not chang…
    Promoter
    T7
    Available Since
    May 15, 2024
    Availability
    Academic Institutions and Nonprofits only
    208637-plasmid-map-sequence-id-433646

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  50. pET28-RPL3-1~41-mEGFP(A206K)-TwStrep

    Plasmid
    #208628
    Purpose
    for E. coli expression of truncated human RPL3 (ENST00000216146), aa1~41, fused with mEGFP(A206K) and Twin Strep tag
    Depositor
    Quanfu Ma
    Insert
    human RPL3 (ENST00000216146) aa 1~41 (RPL3 Human)
    Tags
    6xHis, Twin Strep, and mEGFP(A206K)
    Expression
    Bacterial
    Mutation
    only aa 1~41, other residues not included
    Promoter
    T7
    Available Since
    May 15, 2024
    Availability
    Academic Institutions and Nonprofits only
    208628-plasmid-map-sequence-id-433639

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Showing: 1001 - 1050 of 2431 results