We narrowed to 1,409 results for: 4-Oct

1. pC016 - LwCas13a guide expression backbone with U6 promoter

   Plasmid

   #91906

   Purpose

   Backbone for cloning LwCas13a guides under U6 promoter

   Depositor

   Feng Zhang

   Article

   Abudayyeh et al Nature. 2017 Oct 4. doi: 10.1038/nature2

   Has Service

   Cloning Grade DNA

   Insert

   LwCas13a Guide site
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Oct. 4, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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2. qs5

   Plasmid

   #24498

   Depositor

   Bruce Conklin

   Article

   Conklin et al Mol Pharmacol. 1996 Oct . 50(4):885-90.

   Insert

   Gq alpha (Gnaq Mouse)
   

   Use

   Tags

   HA (see comments)

   Expression

   Mammalian

   Mutation

   Replacement of the five carboxyl-terminal amino a…

   Promoter

   Available since

   June 1, 2010

   Availability

   Academic Institutions and Nonprofits only

3. pC034 - LwCas13a-msfGFP-2A-Blast

   Plasmid

   #91924

   Purpose

   Expresses active LwCas13a for mammalian RNA targeting with Blasticidin selection marker

   Depositor

   Feng Zhang

   Article

   Abudayyeh et al Nature. 2017 Oct 4. doi: 10.1038/nature2

   Insert

   LwCas13a
   

   Use

   CRISPR and Lentiviral

   Tags

   NLS and msfGFP-NLS-3xHA, P2A, Blasticidin

   Expression

   Mammalian

   Mutation

   Promoter

   EFS

   Available since

   Nov. 17, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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4. pC035 - dLwCas13a-msfGFP

   Plasmid

   #91925

   Purpose

   Expresses catalytically inactive LwCas13a for mammalian RNA targeting

   Depositor

   Feng Zhang

   Article

   Abudayyeh et al Nature. 2017 Oct 4. doi: 10.1038/nature2

   Insert

   dLwCas13a
   

   Use

   CRISPR

   Tags

   NLS-3xHA and NLS-msfGFP

   Expression

   Mammalian

   Mutation

   R488A and R1060A

   Promoter

   EF-1a

   Available since

   Nov. 16, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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5. pU6-Sp-pegRNA-RNF2_+5GtoT

   Plasmid

   #135957

   Purpose

   S. pyogenes pegRNA for +5 G to T edit at the RNF2 site of human cells using prime editing

   Depositor

   David Liu

   Article

   Anzalone et al Nature. 2019 Oct 21. pii: 10.1038/s41586

   Insert

   RNF2_5GtoT pegRNA
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   See manuscript

   Promoter

   U6

   Available since

   Jan. 2, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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6. pC0056 - LwCas13a-msfGFP-NES

   Plasmid

   #105815

   Purpose

   Expresses active LwaCas13a-NES for mammalian RNA targeting

   Depositor

   Feng Zhang

   Article

   Abudayyeh et al Nature. 2017 Oct 4. doi: 10.1038/nature2

   Insert

   LwCas13a
   

   Use

   CRISPR and Lentiviral

   Tags

   NES and msfGFP-NES-3xHA

   Expression

   Mammalian

   Mutation

   Promoter

   EF-1a

   Available since

   Feb. 14, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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7. pMTTH+H1.4

   Plasmid

   #157796

   Purpose

   Expression of human linker histone H1.4

   Depositor

   Michael Rosen

   Article

   Gibson et al Cell. 2019 Oct 3;179(2):470-484.e21. doi

   Insert

   H1.4 (H1-4 Human)
   

   Use

   Tags

   His6 and MBP

   Expression

   Bacterial

   Mutation

   Promoter

   pTAC

   Available since

   Aug. 5, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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8. pcDNA1 Flag ATXN1[85Q]

   Plasmid

   #33237

   Depositor

   Huda Zoghbi

   Article

   Skinner et al Nature. 1997 Oct 30;389(6654):971-4.

   Insert

   Ataxin-1 (ATXN1 Human)
   

   Use

   Tags

   Flag

   Expression

   Mammalian

   Mutation

   insert contains a stretch of 85 Glutamines and no…

   Promoter

   CMV

   Available since

   July 22, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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9. bNS2B47NS3

   Plasmid

   #155320

   Purpose

   co-expression of DENV4 47 peptides from NS2B cofactor and NS3 full length

   Depositor

   Dahai Luo

   Article

   Phoo et al Antiviral Res. 2020 Oct;182:104900. doi:

   Insert

   Co expression of DENV4 NS3 full length protein with 47 peptides from NS2B cofactor
   

   Use

   Tags

   His tag

   Expression

   Bacterial

   Mutation

   WT

   Promoter

   T7

   Available since

   Nov. 30, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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10. pcDNA1 Flag ATXN1[30Q]

    Plasmid

    #33236

    Depositor

    Huda Zoghbi

    Article

    Skinner et al Nature. 1997 Oct 30;389(6654):971-4.

    Insert

    Ataxin-1 (ATXN1 Human)
    

    Use

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    insert contains a stretch of 29 amino acids (27 Q…

    Promoter

    CMV

    Available since

    July 18, 2014

    Availability

    Academic Institutions and Nonprofits only

11. SID4x-dCas9-KRAB

    Plasmid

    #106399

    Purpose

    Expresses catalytically inactive dCas9 with N-terminal fusion of Sin3a Interacting Domain (SID) and C-terminal fusion of repressive KRAB domain

    Depositor

    Jason Gertz

    Article

    Carleton et al Cell Syst. 2017 Oct 25;5(4):333-344.e5.

    Inserts

    SID4x (MXD1 Human)
    KRAB (ZNF10 Human)
    

    Use

    Tags

    FLAG, HA, and NLS

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    April 3, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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12. pU6-sp-sgRNA-RNF2_+41nick

    Plasmid

    #135958

    Purpose

    S. pyogenes sgRNA for +41 nick in RNF2

    Depositor

    David Liu

    Article

    Anzalone et al Nature. 2019 Oct 21. pii: 10.1038/s41586

    Insert

    RNF2_+41 nicking sgRNA
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    See manuscript

    Promoter

    U6

    Available since

    Jan. 2, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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13. pRK5-GRK6

    Plasmid

    #32693

    Depositor

    Robert Lefkowitz

    Article

    Pitcher et al J Biol Chem. 1996 Oct 4. 271(40):24907-1

    Insert

    GRK6 (GRK6 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Feb. 21, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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14. pB[vasa-Cas9 PUb-ZsGreen]

    Plasmid

    #169012

    Purpose

    For germline transformation. Expresses Cas9 in germ cells and ZsGreen marker in all cells.

    Depositor

    Max Scott

    Article

    Kandul et al CRISPR J. 2021 Oct;4(5):739-751. doi: 10

    Insert

    Cas9
    

    Use

    Piggybac

    Tags

    Expression

    Insect

    Mutation

    Promoter

    Drosophila melanogaster vasa

    Available since

    April 11, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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15. pcDNA1 Flag ATXN1[2Q]

    Plasmid

    #33235

    Depositor

    Huda Zoghbi

    Article

    Skinner et al Nature. 1997 Oct 30;389(6654):971-4.

    Insert

    Ataxin-1 (ATXN1 Human)
    

    Use

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    insert contains 2Q (2 glutamines) and there isno …

    Promoter

    CMV

    Available since

    April 17, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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16. pC028 - LbfCas13a from Listeriaceae bacterium FSL M6-0635

    Plasmid

    #91918

    Purpose

    Expresses LbfCas13a for bacterial expression and contains backbone for spacer cloning

    Depositor

    Feng Zhang

    Article

    Abudayyeh et al Nature. 2017 Oct 4. doi: 10.1038/nature2

    Insert

    LbfCas13a and guide expression cassette
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Oct. 16, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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17. pB[nos-Cas9 PUb-ZsGreen]

    Plasmid

    #169011

    Purpose

    For germline transformation. Expresses Cas9 in germ cells and ZsGreen marker in all cells.

    Depositor

    Max Scott

    Article

    Kandul et al CRISPR J. 2021 Oct;4(5):739-751. doi: 10

    Insert

    Cas9
    

    Use

    Piggybac

    Tags

    Expression

    Insect

    Mutation

    Promoter

    Drosophila melanogaster nanos

    Available since

    April 11, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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18. pAXEF-Rap1-E63(GTP)

    Plasmid

    #32698

    Depositor

    Vicki Boussiotis

    Article

    Lafuente et al Dev Cell. 2004 Oct;7(4):585-95.

    Insert

    RAP1 (RAP1A Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    E63: Consitutively GTP-bound

    Promoter

    EF promoter

    Available since

    Dec. 5, 2011

    Availability

    Academic Institutions and Nonprofits only

    

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19. pET22b-mEB1-eGFP-6xHis

    Plasmid

    #130981

    Purpose

    Bacterial expression of mouse EB1-GFP-6xHis for protein purification.

    Depositor

    Anne Straube

    Article

    Roth et al J Cell Sci. 2018 Oct 31;132(4). pii: jcs

    Insert

    EB1-GFP-6xHis (Mapre1 Mouse)
    

    Use

    Tags

    eGFP-6xHis

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Oct. 21, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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20. pMIR-His-Halo-Tev-PTEN

    Plasmid

    #58241

    Purpose

    expresses halo tagged PTEN protein

    Depositor

    Yimon Aye

    Article

    Fang et al J Am Chem Soc. 2013 Oct 2;135(39):14496-

    Insert

    phosphatase and tensin homolog (PTEN Human)
    

    Use

    Tags

    His-Halo-Tev

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Aug. 15, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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