We narrowed to 1,336 results for: AG

1. pJC45BS104 Y507W

   Plasmid

   #1305

   Depositor

   Susan Lindquist

   Article

   Cashikar et al Mol Cell 2002 Apr;9(4):751-60.

   Insert

   Hsp104 Y507W (HSP104 Budding Yeast)
   

   Use

   Tags

   10X His tag

   Expression

   Bacterial

   Mutation

   Changed Tyr 507 to Trp

   Promoter

   Available since

   Jan. 6, 2005

   Availability

   Academic Institutions and Nonprofits only

   

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2. pAGK62

   Plasmid

   #228122

   Purpose

   Express node 1531 retron with split retron ncRNA and gRNA to edit (10bp barcode) EMX1 gene in human cells

   Depositor

   Seth Shipman

   Article

   Khan et al Nat Biotechnol. 2024 Sep 17. doi: 10.103

   Insert

   Retron node 1531 split retron ncRNA and gRNA to edit EMX1 gene (10bp barcode) in human cells
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CAG for RT, U6/H1 for ncRNA/gRNA

   Available since

   Dec. 5, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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3. Snap-Omp25

   Plasmid

   #69599

   Purpose

   Snap-tag substrates with two different fluorophores (red and green) sequentially to separately label young and old mitochondria

   Depositor

   David Sabatini

   Article

   Katajisto et al Science. 2015 Apr 17;348(6232):340-3. do

   Insert

   Snap-Omp25
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Dec. 16, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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4. HDM_VSV_G

   Plasmid

   #204156

   Purpose

   VSV-G expression plasmid

   Depositor

   Jesse Bloom

   Article

   Dadonaite et al Cell. 2023 Mar 16;186(6):1263-1278.e20.

   Insert

   VSV-G
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Aug. 16, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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5. pC1-SypHer3s

   Plasmid

   #108118

   Purpose

   Ratiometric pH probe with enhanced brightness for mammalian cells expression.

   Depositor

   Vsevolod Belousov

   Article

   Ermakova et al Chem Commun (Camb). 2018 Mar 2. doi: 10.

   Insert

   SypHer3s
   

   Use

   Tags

   ratiometric YFP

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   April 3, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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6. pCS2+ GFP-2xESP3I-IRES-mCherry

   Plasmid

   #230996

   Purpose

   Protein stability reporter construct for transient expression in mammalian cells. Stability of N-terminal GFP-fusion protein can be assessed by flow cytometry by normalizing to mCherry expression.

   Depositor

   Michael Rape

   Article

   Manford et al Cell. 2020 Oct 1;183(1):46-61.e21. doi:

   Type

   Empty backbone

   Use

   Tags

   AcGFP1

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Jan. 14, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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7. pAGK61

   Plasmid

   #228121

   Purpose

   Express node 1467 retron with split retron ncRNA and gRNA to edit (10bp barcode) EMX1 gene in human cells

   Depositor

   Seth Shipman

   Article

   Khan et al Nat Biotechnol. 2024 Sep 17. doi: 10.103

   Insert

   Retron node 1467, split retron ncRNA and gRNA to edit EMX1 gene (10bp barcode) in human cells
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CAG for RT, U6/H1 for ncRNA/gRNA

   Available since

   Dec. 5, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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8. pAP114

   Plasmid

   #120799

   Purpose

   E. coli-C. difficile shuttle vector for xylose-inducible expression in C. difficile; Pxyl driving expression of red fluorescent protein (mCherryOpt)

   Depositor

   Craig Ellermeier, David S. Weiss

   Article

   Muh et al J Bacteriol. 2019 Feb 11. pii: JB.00711-

   Insert

   Pxyl::mCherryOpt
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   1.4 kb xylR-Pxyl DNA fragment from C. difficile R…

   Promoter

   Pxyl

   Available since

   Feb. 12, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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9. pRS423-GPD-wrmScarlet1-10-CYC1 terminator

   Plasmid

   #158584

   Purpose

   Expresses wrmScarlet1-10 in S. cerevisiae

   Depositor

   Cynthia Kenyon

   Article

   Goudeau et al Genetics. 2021 Apr 15;217(4). pii: 61264

   Insert

   wrmScarlet1-10
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   GPD

   Available since

   Aug. 26, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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10. AMPK-SPARK

    Plasmid

    #226255

    Purpose

    phases-of-separation based activity reporter of AMPK

    Depositor

    Roland Malli

    Article

    Erdogan et al ACS Sens. 2024 Aug 21. doi: 10.1021/acss

    Insert

    AMPK-SPARK
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 18, 2024

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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11. pBabepuroBAF180

    Plasmid

    #41078

    Depositor

    Ramon Parsons

    Article

    Xia et al Cancer Res. 2008 Mar 15;68(6):1667-74.

    Insert

    BAF180 (PBRM1 Human)
    

    Use

    Retroviral

    Tags

    Expression

    Mammalian

    Mutation

    3 silent mutations: 3595 a to g; 4981 a to t; 587…

    Promoter

    Available since

    Jan. 31, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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12. mKate1.31-Lifeact-7

    Plasmid

    #54668

    Purpose

    Localization: Actin, Excitation: 588, Emission: 635

    Depositor

    Michael Davidson

    Article

    Shcherbo et al Biochem J. 2009 Mar 15;418(3):567-74. do

    Type

    Empty backbone

    Use

    Tags

    LifeAct-mKate1.31

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    July 25, 2014

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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13. pSMART-sgRNA (Sp)

    Plasmid

    #80427

    Purpose

    U6 promoter driven sgRNA expression plasmid. Annealed oligonucleotides encoding crRNA sequence are ligated into BbsI site.

    Depositor

    Melissa Little, Sara Howden

    Article

    Howden et al Stem Cell Reports. 2016 Sep 13;7(3):508-

    Type

    Empty backbone

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 15, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pQE30-butelase1

    Plasmid

    #115430

    Purpose

    Expresses the macrocyclizing asparaginyl endopeptidase butelase 1 in E. coli

    Depositor

    Joshua Mylne

    Article

    James et al Plant J. 2019 Feb 21. doi: 10.1111/tpj.1

    Insert

    butelase 1
    

    Use

    Tags

    6-His

    Expression

    Mutation

    Promoter

    T5

    Available since

    Feb. 19, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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15. mKate1.31-pBAD

    Plasmid

    #54827

    Purpose

    Localization: Protein Expression Vector , Excitation: 588, Emission: 635

    Depositor

    Michael Davidson

    Article

    Shcherbo et al Biochem J. 2009 Mar 15;418(3):567-74. do

    Type

    Empty backbone

    Use

    Tags

    6xHis and mKate1.31

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    July 25, 2014

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

16. pCD86-EGFP

    Plasmid

    #133858

    Purpose

    Mammalian expression of CD86 C-terminally tagged with EGFP

    Depositor

    Dave Piston

    Article

    Foust et al Biophys J. 2019 Nov 5;117(9):1764-1777.

    Insert

    CD86 (CD86 Human)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 8, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pEGFP-GNB1

    Plasmid

    #133856

    Purpose

    Mammalian expression of GNB1 N-terminally tagged with EGFP

    Depositor

    Dave Piston

    Article

    Foust et al Biophys J. 2019 Nov 5;117(9):1764-1777.

    Insert

    GNB1 (GNB1 Human)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 8, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pDNR-SpCas9

    Plasmid

    #80425

    Purpose

    Used for in vitro transcription of SpCas9 mRNA.

    Depositor

    Melissa Little, Sara Howden

    Article

    Howden et al Stem Cell Reports. 2016 Sep 13;7(3):508-

    Insert

    SpCas9
    

    Use

    CRISPR

    Tags

    none

    Expression

    Mutation

    codon optimized for human

    Promoter

    T7

    Available since

    Aug. 15, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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19. pTRE3G-iRFP-B1-timestamp_A

    Plasmid

    #159924

    Purpose

    Timestamp Template A inserted into the 3’ UTR of iRFP between bActin Zipcode element and WPRE, with RNA barcode TGC. Also includes a xrRNA element in the 5’ UTR. AKA 147B1.

    Depositor

    Fei Chen

    Article

    Rodriques et al Nat Biotechnol. 2020 Oct 19. pii: 10.103

    Insert

    iRFP-Timestamp A
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    TRE3G

    Available since

    Dec. 2, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pAGK112

    Plasmid

    #228127

    Purpose

    Express node 1531 retron with split retron ncRNA and gRNA to edit (SNIP + PAM recode) HEK4 gene in human cells

    Depositor

    Seth Shipman

    Article

    Khan et al Nat Biotechnol. 2024 Sep 17. doi: 10.103

    Insert

    Retron node 1531 split retron ncRNA and gRNA to edit HEK4 gene (SNP + PAM recode) in human cells
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CAG for RT, U6/H1 for ncRNA/gRNA

    Available since

    Dec. 6, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge