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We narrowed to 12,214 results for: LEA

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Showing: 401 - 420 of 12214 results

  6. M-ST1n-VP64

    Plasmid
    #48675
    Purpose
    Mammalian ST1-VP64 nuclease-null Cas9 activator expression, human optimized
    Depositor
    George Church
    Publication
    Esvelt et al Nat Methods. 2013 Sep 29. doi: 10.1038/n
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    Cas9-VP64, nuclease-null
    Use
    CRISPR
    Tags
    NLS and VP64
    Mutation
    nuclease-null (D9A D598A H599A N622A)
    Promoter
    CMV
    Available since
    Oct. 16, 2013
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    48675-plasmid-map-sequence-id-71850

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  7. pmCherry-C1 R62D actin-3XNLS P2A mCherry

    Plasmid
    #58477
    Purpose
    Expresses nuclear-targeted non-polymerizing R62D mutant of human actin, with an mCherry expression reporter after a P2A protease cleavage site, on a CMV promoter
    Depositor
    Dyche Mullins
    Publication
    Belin et al Elife. 2015 Aug 19;4. doi: 10.7554/eLife
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Inserts
    beta actin (ACTB Human)
    3XNLS
    P2A cleavage site
    Tags
    mCherry
    Expression
    Mammalian
    Mutation
    Changed Arginine 62 to Aspartic Acid
    Promoter
    CMV
    Available since
    Sept. 18, 2015
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    58477-plasmid-map-sequence-id-90386

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  10. pEGFPC1-Cerulean3-Cerulean3-FLARE-D1ER

    Plasmid
    #123354
    Purpose
    Cyan-fluorescent homoFRET/anisotropy-based genetically encoded calcium indicator, targeted to endoplasmic reticulum lumen.
    Depositor
    Jin Zhang
    Publication
    Ross et al eLife. 2018 Jul 3;7. pii: 35458. doi: 10
    Insert
    cp-moxCerulean3-moxCerulean3-FLARE-D1ER
    Tags
    C-terminal ER retention signal (KDEL), cp-moxCeru…
    Expression
    Mammalian
    Promoter
    CMV
    Available since
    Sept. 16, 2020
    Availability
    Academic Institutions and Nonprofits only
    123354-plasmid-map-sequence-id-311156

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  11. pcDNA3-Cerulean3-Cerulean3-FLARE-CKAR

    Plasmid
    #123346
    Purpose
    Cyan-fluorescent homoFRET/anisotropy-based biosensor for monitoring Protein Kinase C activity.
    Depositor
    Jin Zhang
    Publication
    Ross et al eLife. 2018 Jul 3;7. pii: 35458. doi: 10
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    Cerulean3-Cerulean3-FLARE-CKAR
    Tags
    6xHIS, Cerulean3, T7 tag (gene 10 leader), and Xp…
    Expression
    Mammalian
    Promoter
    CMV
    Available since
    July 28, 2020
    Availability
    Academic Institutions and Nonprofits only
    123346-plasmid-map-sequence-id-307099

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  12. pcDNA3-Cerulean3-Cerulean3-FLARE-EKAR-EV

    Plasmid
    #123342
    Purpose
    Cyan-fluorescent homoFRET/anisotropy-based biosensor for monitoring ERK activity.
    Depositor
    Jin Zhang
    Publication
    Ross et al eLife. 2018 Jul 3;7. pii: 35458. doi: 10
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    Cerulean3-Cerulean3-FLARE-EKAR-EV
    Tags
    6xHIS, Cerulean3, T7 tag (gene 10 leader), and Xp…
    Expression
    Mammalian
    Promoter
    CMV
    Available since
    July 28, 2020
    Availability
    Academic Institutions and Nonprofits only
    123342-plasmid-map-sequence-id-307086

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  14. pCS2+HyPer7-NLS

    Plasmid
    #136468
    Purpose
    Mammalian expression of nucleus targeted ultrasensitive hydrogen peroxide indicator HyPer7 for optical imaging
    Depositor
    Vsevolod Belousov
    Publication
    Pak et al Cell Metab. 2020 Mar 3;31(3):642-653.e6.
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    HyPer7
    Tags
    Triple nuclear localization signal of SV40 (simia…
    Expression
    Mammalian
    Promoter
    CMV, SP6
    Available since
    Aug. 18, 2020
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    136468-plasmid-map-sequence-id-308715

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  15. pAd/WT1-IRES-nAmCyan

    Plasmid
    #29756
    Depositor
    Edward McCabe
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    Wilms tumor 1 (WT1 Human)
    Tags
    AmCyan, IRES, and Nuclear localization sequence
    Expression
    Mammalian
    Mutation
    No mutations; AmCyan is nuclear localized; WT1 an…
    Available since
    Oct. 5, 2011
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    29756-plasmid-map-sequence-id-137669

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  17. pcDNA3.1(+)_U6 tRNAPyl_CMV NESPylRS(AF)_IRES_eRF1(E55D)-HA

    Plasmid
    #182653
    Purpose
    Encodes codon-optimized AF mutant of M. mazei pyrrolysine (Pyl) tRNA synthetase fused to a nuclear export signal (NESPylRSAF),tRNACUAPyl & eRF1E55D used for amber codon suppression in mammalian cells
    Depositor
    Ivana Nikić-Spiegel
    Publication
    Arsic et al Nat Commun. 2022 Jan 14;13(1):314. doi:
    Inserts
    codon-optimized Y306A/Y384F (AF) double mutant of Methanosarcina mazei-derived pyrrolysine (Pyl) tRNA synthetase
    PylT
    mutant eukaryotic release factor 1
    Tags
    HA tag and nuclear export signal (NES)
    Expression
    Mammalian
    Mutation
    E55D and Y306A/Y384F (AF) double mutant of Methan…
    Promoter
    CMV and U6
    Available since
    June 16, 2022
    Availability
    Academic Institutions and Nonprofits only
    182653-plasmid-map-sequence-id-363026

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  18. pOPINE GFP nanobody:Halo:His6

    Plasmid
    #111090
    Purpose
    Bacterial expression of a fusion protein consisting of a GFP nanobody (Dr. Brett Collins, Addgene plasmid # 49172), the Halo tag and a His6 tag.
    Depositor
    Lennart Wirthmueller
    Publication
    Chen et al Front Plant Sci. 2018 Nov 1;9:1581. doi:
    Insert
    GFP Nanobody
    Tags
    Halo and His6
    Expression
    Bacterial, Insect, and Mamm…
    Promoter
    T7 promoter
    Available since
    June 20, 2018
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    111090-plasmid-map-sequence-id-264731

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  19. pmCherry-C1 actin-3XNLS P2A mCherry

    Plasmid
    #58475
    Purpose
    Expresses nuclear-targeted human actin, with an mCherry expression reporter after a P2A protease cleavage site, on a CMV promoter
    Depositor
    Dyche Mullins
    Publication
    Belin et al Elife. 2015 Aug 19;4. doi: 10.7554/eLife
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Inserts
    beta actin (ACTB Human)
    3XNLS
    P2A cleavage site
    Tags
    mCherry
    Expression
    Mammalian
    Promoter
    CMV
    Available since
    Sept. 18, 2015
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    58475-plasmid-map-sequence-id-90385

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Showing: 401 - 420 of 12214 results