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pCS2-UTX-F-MT2

Plasmid

#40619

Purpose

Expression of enzyme-dead UTX in mammalian cells

Depositor

Kai Ge

Article

Wang et al Proc Natl Acad Sci U S A. 2012 Sep 4.

Insert

UTX (KDM6A Human)

Tags

6xMyc and Flag

Expression

Mammalian

Mutation

changed Histidine 1146 and Glutamic Acid 1148 to …

Promoter

CMV

Available Since

Nov. 5, 2012

Availability

Academic Institutions and Nonprofits only
pcDNA3 Barr2 flag

Plasmid

#14685

Depositor

Robert Lefkowitz

Article

Laporte et al Proc Natl Acad Sci U S A. 1999 Mar 30. 9

Insert

beta-arrestin 2 (Arrb2 Rat)

Tags

flag

Expression

Mammalian

Available Since

June 19, 2007

Availability

Academic Institutions and Nonprofits only
pcDNA3-LplA(W37V) Coumarin PRIME ligase

Plasmid

#25845

Depositor

Alice Ting

Article

Uttamapinant et al Proc Natl Acad Sci U S A. 2010 Jun 15. 1

Insert

LplA-W37V

Tags

Flag and His

Expression

Mammalian

Mutation

W37V

Available Since

July 27, 2010

Availability

Academic Institutions and Nonprofits only

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FUW-SO

Plasmid

#20329

Depositor

Rudolf Jaenisch

Article

Carey et al Proc Natl Acad Sci U S A. 2009 Jan 6. 10

Insert

Sox2-F2A-Oct4

Use

Lentiviral

Expression

Mammalian

Available Since

Feb. 23, 2009

Availability

Academic Institutions and Nonprofits only

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pPRC6-ProC

Plasmid

#198430

Purpose

mkate2 fluorescent reporter gene under control of promoter ProC. Used as a control plasmid in reporter gene assays.

Depositor

Eveline Peeters

Article

Bernauw et al Microbiologyopen. 2023 Jun;12(3):e1356.

Insert

mKate2

Use

Synthetic Biology

Expression

Bacterial

Promoter

ProC and no promoter

Available Since

March 22, 2023

Availability

Academic Institutions and Nonprofits only

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pCEFL3xHAmGli2

Plasmid

#37671

Depositor

Philip Beachy

Article

Kim et al Proc Natl Acad Sci U S A. 2009 Dec 22;10

Insert

Gli2 (Gli2 Mouse)

Tags

3xHA

Expression

Mammalian

Promoter

EF1a

Available Since

July 10, 2012

Availability

Academic Institutions and Nonprofits only

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pLV-neo-CD2-FAK

Plasmid

#37013

Depositor

Bob Weinberg

Article

Shibue et al Proc Natl Acad Sci U S A. 2009 Jun 23;10

Insert

CD2-FAK (CD2, PTK2 Human)

Use

Lentiviral

Expression

Mammalian

Promoter

EF1a

Available Since

Oct. 19, 2012

Availability

Academic Institutions and Nonprofits only

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pCAG-Fab_8D3_2_H-H6

Plasmid

#176075

Purpose

Express His6 tagged heavy chain of Fab_8D3_2 in mammalian cells

Depositor

Tom Rapoport

Article

Wu et al Proc Natl Acad Sci U S A. 2021 Oct 12;11

Insert

His6 tagged heavy chain of Fab_8D3_2

Tags

His tag

Expression

Mammalian

Promoter

CAG promoter

Available Since

Oct. 12, 2021

Availability

Academic Institutions and Nonprofits only

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pCAG-Fab_8D3_2_L

Plasmid

#176076

Purpose

Express light chain of Fab_8D3_2 in mammalian cells

Depositor

Tom Rapoport

Article

Wu et al Proc Natl Acad Sci U S A. 2021 Oct 12;11

Insert

Light chain of Fab_8D3_2

Expression

Mammalian

Promoter

CAG promoter

Available Since

Oct. 12, 2021

Availability

Academic Institutions and Nonprofits only

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mRFP1-N1

Plasmid

#54635

Purpose

Localization: N1 Cloning Vector, Excitation: 584, Emission: 607

Depositor

Robert Campbell, Michael Davidson, Roger Tsien

Article

Campbell et al Proc Natl Acad Sci U S A. 2002 Jun 11;99

Type

Empty backbone

Tags

mRFP1

Expression

Mammalian

Available Since

June 11, 2014

Availability

Academic Institutions and Nonprofits only

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pET28 MBP_PrA/G-H6

Plasmid

#176077

Purpose

Express His6 tagged MBP_PrA/G in Escherichia coli

Depositor

Tom Rapoport

Article

Wu et al Proc Natl Acad Sci U S A. 2021 Oct 12;11

Insert

MBP PrA/G-H6

Tags

His tag

Expression

Bacterial

Promoter

T7 Promoter

Available Since

Oct. 21, 2021

Availability

Academic Institutions and Nonprofits only

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pLV.PGK.mLmx1a

Plasmid

#33013

Depositor

Malin Parmar

Article

Pfisterer et al Proc Natl Acad Sci U S A. 2011 Jun 21;10

Insert

LIM homeobox transcription factor 1 alpha (Lmx1a Mouse)

Use

Lentiviral

Expression

Mammalian

Available Since

Jan. 19, 2012

Availability

Academic Institutions and Nonprofits only

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MSCVhygro-F-Ezh2-F667I

Plasmid

#24927

Depositor

Kai Ge

Article

Wang et al Proc Natl Acad Sci U S A. 2010 Apr 20. 1

Insert

Ezh2 (EZH2 Mouse)

Use

Retroviral

Tags

FLAG

Expression

Mammalian

Mutation

Phenylanaline(F)667 mutated to Isoleucine(I)

Available Since

July 15, 2010

Availability

Academic Institutions and Nonprofits only
pCAG-FAST-p10

Plasmid

#89152

Purpose

Express p10-FAST in mammalian cells

Depositor

Takeshi Kobayashi

Article

Kanai et al Proc Natl Acad Sci U S A. 2017 Jan 30. p

Insert

NBV p10-FAST

Expression

Mammalian

Promoter

CAG promotor

Available Since

April 10, 2017

Availability

Academic Institutions and Nonprofits only

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pCMV5 HA-TBRII (delta Cyt)

Plasmid

#14051

Depositor

Joan Massague

Article

Siegel et al Proc Natl Acad Sci U S A. 2003 Jul 8. 10

Insert

TGF beta rec II (delta cyt) (TGFBR2 Human)

Tags

HA (PYDVPDYA)

Expression

Mammalian

Mutation

Dominant negative mutant. A stop codon was introd…

Available Since

May 10, 2007

Availability

Academic Institutions and Nonprofits only

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scFLARE2

Plasmid

#158700

Purpose

Neuronal expression of scFLARE2 component

Depositor

Alice Ting

Article

Sanchez et al Proc Natl Acad Sci U S A. 2020 Dec 15. p

Insert

scFLARE2

Use

AAV

Tags

HA, Flag, VP16

Expression

Mammalian

Mutation

Truncated form of TEV protease (220-242 amino aci…

Promoter

Syn

Available Since

Jan. 6, 2021

Availability

Academic Institutions and Nonprofits only

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mPlum-pBAD

Plasmid

#54564

Purpose

Localization: Protein Expression Vector , Excitation: 590, Emission: 649

Depositor

Michael Davidson, Roger Tsien

Article

Wang et al Proc Natl Acad Sci U S A. 2004 Nov 30;10

Type

Empty backbone

Tags

6xHis and mPlum

Expression

Bacterial

Available Since

July 25, 2014

Availability

Academic Institutions and Nonprofits only

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pcDNA3-6XHis-human SUMO 1

Plasmid

#133770

Purpose

express 6Xhis- SUMO 1 protein

Depositor

Martine Roussel

Article

Tago et al Proc Natl Acad Sci U S A. 2005 May 24. 1

Insert

SUMO 1 (SUMO1 Human)

Tags

6XHis

Expression

Mammalian

Promoter

CMV

Available Since

Dec. 6, 2019

Availability

Academic Institutions and Nonprofits only

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pEntr-GATA4flbio

Plasmid

#32937

Depositor

William Pu

Article

He et al Proc Natl Acad Sci U S A. 2011 Apr 5. 10

Insert

GATA4 (Gata4 Mouse)

Use

Gateway Cloning

Tags

flag-bio

Promoter

none

Available Since

Jan. 10, 2012

Availability

Academic Institutions and Nonprofits only

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SpyLigase

Plasmid

#51722

Purpose

Plasmid for recombinant expression in bacteria of SpyLigase. The Howarth lab has created two improved peptide ligation enzymes. See depositor comments below!

Depositor

Mark Howarth

Article

Fierer et al Proc Natl Acad Sci U S A. 2014 Mar 17.

Insert

SpyLigase

Tags

His6 Tag

Expression

Bacterial

Promoter

T7

Available Since

May 1, 2014

Availability

Academic Institutions and Nonprofits only

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