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AAV-Cre-GFP

Plasmid

#68544

Purpose

AAV expression of Cre-GFP fusion

Depositor

Eric Nestler

Article

Kennedy et al Nat Neurosci. 2013 Apr;16(4):434-40. doi

Insert

Cre

Use

AAV and Cre/Lox

Tags

EGFP

Expression

Mammalian

Promoter

CMV

Available Since

Sept. 3, 2015

Availability

Academic Institutions and Nonprofits only

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pGEX-4T-1 3xHA-MEK1-DD

Plasmid

#47576

Purpose

Bacterial expression plasmid for GST-3xHA-tagged constitutively active MEK1

Depositor

Kevin Janes

Article

Bose et al Mol Cell Proteomics. 2013 Mar;12(3):797-

Insert

MEK1-DD (Map2k1 Mouse)

Tags

3xHA and GST

Expression

Bacterial

Mutation

Ser218 to Asp, Ser221 to Asp

Available Since

Aug. 28, 2013

Availability

Academic Institutions and Nonprofits only
p(A)n-PAUSE

Plasmid

#118068

Purpose

Transcription Blocker - Synthetic polyadenylation signal & RNA polymerase II transcriptional pause signal from the human α2 globin gene

Depositor

Koen Venken

Article

Sarrion-Perdigones et al Nat Commun. 2019 Dec 13;10(1):5710. doi:

Insert

Synthetic polyadenylation signal & RNA polymerase II transcriptional pause signal from the human α2 globin gene

Use

Synthetic Biology

Expression

Mammalian

Available Since

Dec. 16, 2019

Availability

Academic Institutions and Nonprofits only

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pEasyG3_nat

Plasmid

#184918

Purpose

Template to generate via PCR two gRNAs for expression in S. cerevisiae. The PCR product from pEasyG3_nat recombines in vivo with a PCR product from pEasyG3_mic.

Depositor

Jeferson Gross

Article

Jacobus et al ACS Synth Biol. 2022 Nov 18;11(11):3886-

Insert

gRNA scaffold

Use

CRISPR

Expression

Bacterial

Available Since

Oct. 28, 2022

Availability

Academic Institutions and Nonprofits only

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pEasyG3_mic

Plasmid

#184920

Purpose

Template to generate via PCR two gRNAs for expression in S. cerevisiae. The PCR product from pEasyG3_mic recombines in vivo with a PCR product from pEasyG3_zeo/nat/hph.

Depositor

Jeferson Gross

Article

Jacobus et al ACS Synth Biol. 2022 Nov 18;11(11):3886-

Insert

gRNA scaffold

Use

CRISPR

Expression

Bacterial

Available Since

Oct. 28, 2022

Availability

Academic Institutions and Nonprofits only

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hCMV-IE1:Renilla

Plasmid

#118066

Purpose

Transcriptional unit for constitutive expression of Renilla in mammalian cells

Depositor

Koen Venken

Article

Sarrion-Perdigones et al Nat Commun. 2019 Dec 13;10(1):5710. doi:

Insert

CMV:GreenRenilla:bGHF

Use

Luciferase and Synthetic Biology; Assembly of gb2…

Expression

Mammalian

Promoter

CMV

Available Since

Dec. 16, 2019

Availability

Academic Institutions and Nonprofits only

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pRK172-6xHis-0N4R-P301S-tau-HiBiT

Plasmid

#187835

Purpose

Expression of 6xHis-tagged P301S tau of 0N4R isoform with a C-terminal HiBiT tag for bimolecular luminescence with LgBiT.

Depositor

William McEwan

Article

Tuck et al Cell Rep. 2022 May 3;39(5):110776. doi:

Insert

6XHis-0N4R-P301S-tau-HiBiT

Tags

6xHistidine and HiBiT

Expression

Bacterial

Promoter

T7

Available Since

Sept. 22, 2023

Availability

Academic Institutions and Nonprofits only

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pGEX-4T-1 3xFlag-JNK1a1

Plasmid

#47574

Purpose

Bacterial expression plasmid for GST-3xFlag-tagged JNK1a1

Depositor

Kevin Janes

Article

Bose et al Mol Cell Proteomics. 2013 Mar;12(3):797-

Insert

JNK1a1 (MAPK8 Human)

Tags

3xFlag and GST

Expression

Bacterial

Available Since

Aug. 28, 2013

Availability

Academic Institutions and Nonprofits only
pLX302-MKP5-V5 puro

Plasmid

#87770

Purpose

Expresses V5 tagged human MAPK phosphatase 5 (MKP5/DUSP10)

Depositor

Kevin Janes

Article

Shah et al Mol Cell Proteomics. 2017 Apr;16(4 suppl

Insert

MKP5 (DUSP10 Human)

Use

Lentiviral

Tags

V5

Expression

Mammalian

Available Since

March 22, 2017

Availability

Academic Institutions and Nonprofits only

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pGEX-4T-1 3xHA-MKK6-EE

Plasmid

#47579

Purpose

Bacterial expression plasmid for GST-3xHA-tagged constitutively active MKK6

Depositor

Kevin Janes

Article

Bose et al Mol Cell Proteomics. 2013 Mar;12(3):797-

Insert

MKK6-EE (MAP2K6 Human)

Tags

3xHA and GST

Expression

Bacterial

Mutation

Ser207 to Glu, Thr211 to Glu

Available Since

Aug. 28, 2013

Availability

Academic Institutions and Nonprofits only
NEK7_HUMAN_D0

Plasmid

#79735

Purpose

This plasmid encodes the kinase domain of NEK7. Intended for co-expression with Lambda Phosphatase that accompanies this set to enhance bacterial kinase expression.

Depositor

John Chodera, Nicholas Levinson, Markus Seeliger

Article

Albanese et al Biochemistry. 2018 Jul 13. doi: 10.1021/

Insert

NEK7 (NEK7 Human)

Tags

His10-TEV

Expression

Bacterial

Promoter

T7

Available Since

Nov. 21, 2016

Availability

Academic Institutions and Nonprofits only
pRedF

Plasmid

#118057

Purpose

GBPart - RedF Luciferase CDS

Depositor

Koen Venken

Article

Sarrion-Perdigones et al Nat Commun. 2019 Dec 13;10(1):5710. doi:

Insert

Red Firefly Luciferase CDS

Use

Synthetic Biology; Domestication of dna parts for…

Mutation

Red mutant S286Y

Available Since

Dec. 16, 2019

Availability

Academic Institutions and Nonprofits only

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pAAV_hSyn_WiChR_TS_mScarlet_ER

Plasmid

#205997

Purpose

High efficient, K+ selective channelrhodopsin for the inhibition of neurons

Depositor

Peter Hegemann, Johannes Vierock

Article

Vierock et al Sci Adv. 2022 Nov 16:eadd7729.

Has Service

AAV9

Insert

Wobblia Inhibitory Channelrhodopsin

Use

AAV

Tags

TS, mScarlet, ER

Promoter

hSyn

Available Since

Sept. 5, 2023

Availability

Academic Institutions and Nonprofits only

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AAEL010097-Cas9

Plasmid

#100707

Purpose

The promoter of AAEL010097 drive expression of Streptococcus pyogenes Cas9 gene in Aedes aegypti

Depositor

Omar Akbari

Article

Li et al Proc Natl Acad Sci U S A. 2017 Nov 14. p

Inserts

AAEL010097-hspcas9-GFP
Opie2-dsRed

Use

CRISPR

Tags

GFP and dsRED

Expression

Insect

Promoter

AAEL010097 and Opie2

Available Since

Oct. 10, 2017

Availability

Academic Institutions and Nonprofits only

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5xNF-kb_RE::RedF

Plasmid

#124530

Purpose

NFKB RedFirefly luciferase reporter

Depositor

Koen Venken

Article

Sarrion-Perdigones et al Curr Protoc Mol Biol. 2020 Jun;131(1):e1

Insert

NF-κβ RedF reporter

Use

Luciferase and Synthetic Biology

Expression

Mammalian

Available Since

June 17, 2020

Availability

Academic Institutions and Nonprofits only

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tet-pLKO shGATA6 puro

Plasmid

#72615

Purpose

Expresses an inducible short hairpin targeting human GATA6 sequence

Depositor

Kevin Janes

Article

Chitforoushzadeh et al Sci Signal. 2016 Jun 7;9(431):ra59. doi:

Insert

short hairpin GATA binding protein 6

Use

Lentiviral

Tags

None

Expression

Mammalian

Promoter

TRE Tight

Available Since

Aug. 29, 2016

Availability

Academic Institutions and Nonprofits only

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pcDNA3-His:dCas9:nls:10P3

Plasmid

#135982

Purpose

Expresses dCas9 fused to 10 copies of the P3 coiled coil in mammalian cells

Depositor

Roman Jerala

Article

Lebar et al Nat Chem Biol. 2020 Jan 6. pii: 10.1038/

Insert

dCas9 fused to 10 copies of the P3 coiled-coil

Use

CRISPR

Tags

His

Expression

Mammalian

Mutation

D10A; H840A in S.pyogenes Cas9

Promoter

CMV

Available Since

Aug. 19, 2020

Availability

Academic Institutions and Nonprofits only

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p35S:Citrine-TurboID-3xHA

Plasmid

#199243

Purpose

This plasmid could be used to clone gene(protein) of interest in place of Citrine to use in proximity labeling approach in plants.

Depositor

Savithramma Dinesh-Kumar

Article

Zhang et al Nat Commun. 2019 Jul 19;10(1):3252. doi:

Insert

Citrine-TurboID

Tags

3xHA and TurboID-3xHA

Expression

Plant

Promoter

CaMV 35S

Available Since

June 6, 2023

Availability

Academic Institutions and Nonprofits only

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hCMV-IE1:FLuc

Plasmid

#118063

Purpose

Transcriptional unit for constitutive expression of FLuc in mammalian cells

Depositor

Koen Venken

Article

Sarrion-Perdigones et al Nat Commun. 2019 Dec 13;10(1):5710. doi:

Insert

CMV:FLuc:bGHF

Use

Luciferase and Synthetic Biology; Assembly of gb2…

Expression

Mammalian

Available Since

Dec. 16, 2019

Availability

Academic Institutions and Nonprofits only

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pLenti-CMV-MFSD8-Puro

Plasmid

#239954

Purpose

Lentiviral vector to generate MFSD8 stable expressing cell line under CMV promoter

Depositor

Jan Carette

Article

Varanese et al Nature. 2025 Mar 25. doi: 10.1038/s41586

Insert

MFSD8

Use

Lentiviral

Tags

myc-flag

Expression

Mammalian

Available Since

July 14, 2025

Availability

Academic Institutions and Nonprofits only

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