We narrowed to 13,113 results for: CRISPR-Cas9

1. pEG302 22aa SunTag NtDRMcd (no NLS) g4+g10+g18 (FWA)

   Plasmid

   #117168

   Purpose

   CRISPR Cas9 SunTag system to target NtDRMcd (without an NLS) to the FWA locus with three guide RNAs

   Depositor

   Steven Jacobsen

   Article

   Papikian et al Nat Commun. 2019 Feb 13;10(1):729. doi:

   Insert

   g18_U6_g10_U6_g4_U6_NOS_NLS_GB1_noNLS_linker_DRMcd_linker_sfGFP_scFv_UBQ10_Insulator_UBQ10_Ω_dCas9_1xHA_3xNLS_linker_10xGCN4_OCS
   

   Use

   CRISPR and Synthetic Biology

   Tags

   Expression

   Plant

   Mutation

   Promoter

   Available since

   March 19, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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2. AIO-Puro

   Plasmid

   #74630

   Purpose

   All-in-One plasmid encoding dual U6 promoter-driven sgRNAs and Cas9-D10A nickase linked via 2A peptide with puromycin resistant marker to enhance efficient and accurate genome editing

   Depositor

   Steve Jackson

   Article

   Chiang et al Sci Rep. 2016 Apr 15;6:24356. doi: 10.10

   Type

   Empty backbone

   Use

   CRISPR

   Tags

   Puromycin resistant marker

   Expression

   Mammalian

   Mutation

   Promoter

   Cbh

   Available since

   May 6, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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3. pMpGE_En03

   Plasmid

   #71535

   Purpose

   Used to construct a gRNA expression cassette for CRISPR/Cas9 genome editing in Marchantia polymorpha

   Depositor

   Takayuki Kohchi, Sumihare Noji

   Article

   Sugano et al PLoS One. 2018 Oct 31;13(10):e0205117. d

   Type

   Empty backbone

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   Available since

   March 14, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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4. pMpGE010

   Plasmid

   #71536

   Purpose

   For CRISPR/Cas9 genome editing in Marchantia polymorpha

   Depositor

   Takayuki Kohchi

   Article

   Sugano et al PLoS One. 2018 Oct 31;13(10):e0205117. d

   Type

   Empty backbone

   Use

   CRISPR

   Tags

   Expression

   Plant

   Mutation

   Promoter

   Available since

   March 14, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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5. pMpGE011

   Plasmid

   #71537

   Purpose

   For CRISPR/Cas9 genome editing in Marchantia polymorpha

   Depositor

   Takayuki Kohchi

   Article

   Sugano et al PLoS One. 2018 Oct 31;13(10):e0205117. d

   Type

   Empty backbone

   Use

   CRISPR

   Tags

   Expression

   Plant

   Mutation

   Promoter

   Available since

   March 14, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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6. pLSC-5

   Plasmid

   #62889

   Purpose

   Lenti vector for expression of inducible split Cas9

   Depositor

   Feng Zhang

   Article

   Zetsche et al Nat Biotechnol. 2015 Feb 2. doi: 10.1038

   Inserts

   SpCas9(574-1368)
   SpCas9(2-573)
   

   Use

   CRISPR and Lentiviral

   Tags

   FFBP12, FRB, NES PTK2, NLS SV40, and P2A

   Expression

   Mammalian

   Mutation

   *see comment below

   Promoter

   EFS

   Available since

   March 26, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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7. pBLO1808_arC9_2xNLS_human

   Plasmid

   #74491

   Purpose

   Human codon optimized plasmid to express arC9 t2a mCherry w/2xNLS and a sgRNA

   Depositor

   David Savage

   Article

   Oakes et al Nat Biotechnol. 2016 May 2. doi: 10.1038

   Insert

   arC9
   

   Use

   Tags

   t2a mCherry

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   May 20, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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8. pGTag Hygro EGFP nLAP +0

   Plasmid

   #194305

   Purpose

   CRISPR/Cas9-mediated generic protein tagging

   Depositor

   Frank Schnütgen

   Article

   Thone et al Methods. 2019 Jul 15;164-165:59-66. doi:

   Insert

   Hygro-P2A-nLAP(EGFP)
   

   Use

   Bacterial cloning vector

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Feb. 7, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pBC2102-reci

   Plasmid

   #117171

   Purpose

   U6-sgRNA-EFS-Cas9-T2A-mCherry-P2A-Hygro; wtCas9 without NLS

   Depositor

   David Savage

   Article

   Oakes et al Nat Biotechnol. 2016 May 2. doi: 10.1038

   Insert

   reci_wtCas9 without NLS
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   U6 Promoter

   Available since

   Oct. 12, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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10. pGTag Hygro BFP nLAP +0

    Plasmid

    #194317

    Purpose

    CRISPR/Cas9-mediated generic protein tagging

    Depositor

    Frank Schnütgen

    Article

    Thone et al Methods. 2019 Jul 15;164-165:59-66. doi:

    Insert

    Hygro-P2A-nLAP(BFP)
    

    Use

    Bacterial cloning vector

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Sept. 8, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pGTag Hygro EGFP nLAP -2

    Plasmid

    #194322

    Purpose

    CRISPR/Cas9-mediated generic protein tagging

    Depositor

    Frank Schnütgen

    Article

    Thone et al Methods. 2019 Jul 15;164-165:59-66. doi:

    Insert

    Hygro-P2A-nLAP(EGFP)
    

    Use

    Bacterial cloning vector

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Feb. 8, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pGTag Hygro BFP nLAP -1

    Plasmid

    #194324

    Purpose

    CRISPR/Cas9-mediated generic protein tagging

    Depositor

    Frank Schnütgen

    Article

    Thone et al Methods. 2019 Jul 15;164-165:59-66. doi:

    Insert

    Hygro-P2A-nLAP(BFP)
    

    Use

    Bacterial cloning vector

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Feb. 8, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pGTag Hygro EGFP nLAP -1

    Plasmid

    #194321

    Purpose

    CRISPR/Cas9-mediated generic protein tagging

    Depositor

    Frank Schnütgen

    Article

    Thone et al Methods. 2019 Jul 15;164-165:59-66. doi:

    Insert

    Hygro-P2A-nLAP(EGFP)
    

    Use

    Bacterial cloning vector

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Feb. 8, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pGTag Hygro BFP nLAP -0

    Plasmid

    #194323

    Purpose

    CRISPR/Cas9-mediated generic protein tagging

    Depositor

    Frank Schnütgen

    Article

    Thone et al Methods. 2019 Jul 15;164-165:59-66. doi:

    Insert

    Hygro-P2A-nLAP(BFP)
    

    Use

    Bacterial cloning vector

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Feb. 8, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pGTag Hygro EGFP nLAP -0

    Plasmid

    #194320

    Purpose

    CRISPR/Cas9-mediated generic protein tagging

    Depositor

    Frank Schnütgen

    Article

    Thone et al Methods. 2019 Jul 15;164-165:59-66. doi:

    Insert

    Hygro-P2A-nLAP(EGFP)
    

    Use

    Bacterial cloning vector

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Feb. 8, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pGTag Hygro BFP nLAP -2

    Plasmid

    #194325

    Purpose

    CRISPR/Cas9-mediated generic protein tagging

    Depositor

    Frank Schnütgen

    Article

    Thone et al Methods. 2019 Jul 15;164-165:59-66. doi:

    Insert

    Hygro-P2A-nLAP(BFP)
    

    Use

    Bacterial cloning vector

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Feb. 8, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pGTag Hygro BFP nLAP +1

    Plasmid

    #194318

    Purpose

    CRISPR/Cas9-mediated generic protein tagging

    Depositor

    Frank Schnütgen

    Article

    Thone et al Methods. 2019 Jul 15;164-165:59-66. doi:

    Insert

    Hygro-P2A-nLAP(BFP)
    

    Use

    Bacterial cloning vector

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Feb. 7, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pGTag Hygro BFP nLAP +2

    Plasmid

    #194319

    Purpose

    CRISPR/Cas9-mediated generic protein tagging

    Depositor

    Frank Schnütgen

    Article

    Thone et al Methods. 2019 Jul 15;164-165:59-66. doi:

    Insert

    Hygro-P2A-nLAP(BFP)
    

    Use

    Bacterial cloning vector

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Feb. 7, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pGTag Hygro EGFP nLAP +1

    Plasmid

    #194306

    Purpose

    CRISPR/Cas9-mediated generic protein tagging

    Depositor

    Frank Schnütgen

    Article

    Thone et al Methods. 2019 Jul 15;164-165:59-66. doi:

    Insert

    Hygro-P2A-nLAP(EGFP)
    

    Use

    Bacterial cloning vector

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Feb. 6, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pGTag Hygro EGFP nLAP +2

    Plasmid

    #194307

    Purpose

    CRISPR/Cas9-mediated generic protein tagging

    Depositor

    Frank Schnütgen

    Article

    Thone et al Methods. 2019 Jul 15;164-165:59-66. doi:

    Insert

    Hygro-P2A-nLAP(EGFP)
    

    Use

    Bacterial cloning vector

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Feb. 6, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge