We narrowed to 11,742 results for: pcDNA

1. pcDNA5/FRT/TO-GAlpha15-RLuc8

   Plasmid

   #140984

   Purpose

   Encodes a G alpha subunit (GNA15) containing RLuc8 as an optimal component of a BRET2 biosensor for studying heterotrimeric G protein dissociation

   Depositor

   Bryan Roth

   Article

   Olsen et al Nat Chem Biol. 2020 May 4. pii: 10.1038/

   Insert

   GAlpha15-RLuc8 (GNA15 Human)
   

   Use

   Luciferase

   Tags

   Expression

   Mammalian

   Mutation

   RLuc8 and flanking SGGGGS linkers have been inser…

   Promoter

   CMV

   Available since

   June 26, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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2. pcDNA3.1(Hygro) TagBFP-hNucleolin

   Plasmid

   #182592

   Purpose

   expresses bfp-hNucleolin in mammalian cells

   Depositor

   Agnel Sfeir

   Article

   Laprade et al Mol Cell. 2020 Jul 2;79(1):115-126.e6. d

   Insert

   hNucleolin (NCL Human)
   

   Use

   Tags

   BFP

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   April 28, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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3. pcDNA3 CHICA (Nigg HS342)

   Plasmid

   #41153

   Depositor

   Erich Nigg

   Article

   Santamaria et al Curr Biol. 2008 May 20;18(10):723-9.

   Insert

   CHICA (FAM83D Human)
   

   Use

   Tags

   3xMyc and PreScission Site

   Expression

   Mammalian

   Mutation

   V11L

   Promoter

   CMV

   Available since

   June 7, 2013

   Availability

   Academic Institutions and Nonprofits only

4. pcDNA3.1_3xFlagEzh2 RNA binding mutant

   Plasmid

   #173718

   Purpose

   RNA-binding mutant EZH2 expression plasmid with 3XFLAG tag

   Depositor

   Thomas Cech

   Article

   Long et al Nat Genet. 2020 Sep;52(9):931-938. doi:

   Insert

   EZH2 RNA-binding mutant
   

   Use

   Tags

   3XFLAG

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Aug. 26, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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5. pcDNA5-H2B-AID-EYFP

   Plasmid

   #47329

   Purpose

   for plasmid integration using the Flp-In system. AID at C-terminal

   Depositor

   Don Cleveland

   Article

   Holland et al Proc Natl Acad Sci U S A. 2012 Dec 4;109

   Insert

   histone H2B (H2BC21 Human)
   

   Use

   Tags

   AID and EYFP

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Aug. 23, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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6. pcDNA3-FLAG-HA-CAD S1859A

   Plasmid

   #46239

   Purpose

   FLAG-HA-CAD with S1859 phosphorylation site mutated to alanine

   Depositor

   Brendan Manning

   Article

   Ben-Sahra et al Science. 2013 Mar 15;339(6125):1323-8. d

   Insert

   carbamoyl-phosphate synthetase 2, aspartate transcarbamylase, and dihydroorotase (Cad Mouse)
   

   Use

   Tags

   FLAG and HA

   Expression

   Mammalian

   Mutation

   Serine 1859 changed to Alanine (S1859A)

   Promoter

   cmv

   Available since

   Nov. 22, 2013

   Availability

   Academic Institutions and Nonprofits only

7. pcDNA-flag-mKdm4d-polyA

   Plasmid

   #61553

   Purpose

   For mRNA synthesis or expression in mammalian cells

   Depositor

   Yi Zhang

   Article

   Matoba et al Cell. 2014 Nov 6;159(4):884-95. doi: 10.

   Insert

   Kdm4d (Kdm4d Mouse)
   

   Use

   Tags

   flag

   Expression

   Mammalian

   Mutation

   Promoter

   CMV and T7

   Available since

   Feb. 23, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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8. FRT LAMP3 mCRISPRed_mTagBFP2 pcDNA5

   Plasmid

   #159455

   Purpose

   Encodes a lysosome tagret fusion protein of the pH resistant proteins mCRISPRed and mTagBFP2

   Depositor

   Oliver Griesbeck

   Article

   Erdogan et al Cell Chem Biol. 2020 May 21;27(5):610-62

   Insert

   mCRISPRed
   

   Use

   Tags

   mTagBFP2

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Oct. 1, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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9. GeNL(Ca2+)_520/pcDNA3

   Plasmid

   #85204

   Purpose

   luminescent reporter for calcium signaling

   Depositor

   Takeharu Nagai

   Article

   Suzuki et al Nat Commun. 2016 Dec 14;7:13718. doi: 10

   Insert

   GeNL(Ca2+)_520
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   March 19, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pcDNA6A-EGFR ECD (1-644)

    Plasmid

    #42666

    Depositor

    Mien-Chie Hung

    Article

    Hsu et al J Biol Chem. 2007 Apr 6;282(14):10432-40

    Insert

    EGFR ECD (1-644) (EGFR Human)
    

    Use

    Tags

    6xHis and Myc

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 17, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pcDNA-NBid-PhoCl2c-CBid

    Plasmid

    #164051

    Purpose

    Optogenetic manipulation of cell apoptosis using PhoCl2c

    Depositor

    Robert Campbell

    Article

    Lu et al Chem Sci. 2021 May 31;12(28):9658-9672.

    Insert

    NBid-PhoCl2c-CBid
    

    Use

    Tags

    kozak sequence

    Expression

    Mammalian

    Mutation

    Promoter

    CMV promotor

    Available since

    Feb. 19, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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12. pcDNA5/FRT/TO_Beta4Gal-HaloTag7

    Plasmid

    #175536

    Purpose

    Expression of HaloTag7 at the at the Gogli apparatus of the mammalian cells

    Depositor

    Kai Johnsson

    Article

    Frei et al Nat Methods. 2021 Dec 16. pii: 10.1038/s

    Insert

    Beta4Gal-HaloTag7
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Dec. 13, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pcDNA3.1D V5-His-TOPO-KGA.wt

    Plasmid

    #110332

    Purpose

    Mammalian expression of glutaminase isoform KGA (wild type) with V5 and 6xHis tags

    Depositor

    Sandra Martha Gomes Dias

    Article

    Cassago et al Proc Natl Acad Sci U S A. 2012 Jan 24;10

    Insert

    KGA glutaminase (GLS Human)
    

    Use

    Tags

    6xHis and V5

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    June 20, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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14. pcDNA3 deltaN89 Beta-catenin

    Plasmid

    #19288

    Depositor

    Eric Fearon

    Article

    Kolligs et al Mol Cell Biol. 1999 Aug . 19(8):5696-706

    Insert

    beta-catenin (CTNNB1 Human)
    

    Use

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    N-terminal truncation, deleted aa 1-89

    Promoter

    Available since

    Sept. 8, 2008

    Availability

    Academic Institutions and Nonprofits only

15. pcDNA3-FLAG-Synaptojanin 1-145

    Plasmid

    #22291

    Depositor

    Pietro De Camilli

    Article

    Haffner et al FEBS Lett. 1997 Dec 15. 419(2-3):175-80.

    Insert

    Synaptojanin 1_145 (SYNJ1 Human)
    

    Use

    Tags

    Flag

    Expression

    Bacterial and Mammalian

    Mutation

    Promoter

    Available since

    Oct. 9, 2009

    Availability

    Academic Institutions and Nonprofits only

    

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16. pcDNA5 FRT TO-EGFP-G3BP1-WT

    Plasmid

    #136003

    Purpose

    DOX-inducible WT G3BP1 inserted with GFP tagged on the N-terminus and used with the Flp-In T-Rex System

    Depositor

    Anthony Leung

    Article

    Fischer et al Mol Cell. 2020 Apr 2;78(1):70-84.e6. doi

    Insert

    G3BP1 (G3BP1 Human)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Feb. 28, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pcDNA5/FRT/TO-GAlphasS-RLuc8

    Plasmid

    #140980

    Purpose

    Encodes a G alpha subunit (GNAS2) containing RLuc8 as an optimal component of a BRET2 biosensor for studying heterotrimeric G protein dissociation

    Depositor

    Bryan Roth

    Article

    Olsen et al Nat Chem Biol. 2020 May 4. pii: 10.1038/

    Insert

    GAlphasS-RLuc8
    

    Use

    Luciferase

    Tags

    Expression

    Mammalian

    Mutation

    RLuc8 and flanking SGGGGS linkers have been inser…

    Promoter

    CMV

    Available since

    June 26, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pcDNA3.1-Y1175F-VEGFR2-mCherry

    Plasmid

    #139608

    Purpose

    Expresses a mutant (Y1175F) VEGFR2-mCherry fusion protein in mammalian cells

    Depositor

    Michael Welsh

    Article

    Pietila et al Cells. 2019 Dec 15;8(12). pii: cells8121

    Insert

    KDR (KDR Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    July 30, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pcDNA3.1-N-Myc_535-775 USP7

    Plasmid

    #131247

    Purpose

    Mammalian expression of the USP7 UBL1-2 domains with an N-terminal Myc tag.

    Depositor

    Roger Woodgate

    Article

    Ashton et al J Mol Biol. 2021 Jan 22;433(2):166733. d

    Insert

    Ubiquitin carboxyl-terminal hydrolase 7 (USP7 Human)
    

    Use

    Tags

    Myc

    Expression

    Mammalian

    Mutation

    A mutant form of our pcDNA3.1-N-Myc_535-1102 USP7…

    Promoter

    Available since

    Dec. 17, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pcDNA3.1-N-Myc_775-1102 USP7

    Plasmid

    #131250

    Purpose

    Mammalian expression of the USP7 UBL3-5 domains with an N-terminal Myc tag

    Depositor

    Roger Woodgate

    Article

    Ashton et al J Mol Biol. 2021 Jan 22;433(2):166733. d

    Insert

    Ubiquitin carboxyl-terminal hydrolase 7 (USP7 Human)
    

    Use

    Tags

    Myc

    Expression

    Mammalian

    Mutation

    Encodes USP7 UBL domains 3-5 (USP7 amino acids 77…

    Promoter

    Available since

    Dec. 17, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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