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pGCP123-EbpA_g1

Plasmid

#153517

Purpose

sgRNA for ebpA gene (NT) under nisin-inducible nisA promoter, barcoded

Depositor

Kimberly Kline

Publication

Afonina et al mBio. 2020 Oct 20;11(5):e01101-20. doi:

Insert

pnisA-sgRNA(EbpA_g1)-dCas9 scaffold

Use

CRISPR and Synthetic Biology

Expression

Bacterial

Available since

July 28, 2020

Availability

Academic Institutions and Nonprofits only

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pGWB519

Plasmid

#74861

Purpose

Gateway cloning compatible binary vector for C-terminal fusion with 10xMyc (no promoter).

Depositor

Tsuyoshi Nakagawa

Publication

Nakagawa et al Biosci Biotechnol Biochem. 2007 Aug;71(8

Type

Empty backbone

Expression

Plant

Available since

Aug. 4, 2016

Availability

Academic Institutions and Nonprofits only

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pGWB410

Plasmid

#74804

Purpose

Gateway cloning compatible binary vector for C-terminal fusion with FLAG (no promoter).

Depositor

Tsuyoshi Nakagawa

Publication

Nakagawa et al Biosci Biotechnol Biochem. 2007 Aug;71(8

Type

Empty backbone

Expression

Plant

Available since

July 14, 2016

Availability

Academic Institutions and Nonprofits only

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mOrange2-Lysosomes-20

Plasmid

#57968

Purpose

Localization: Lysosome Membrane, Excitation: 549, Emission: 565

Depositor

Michael Davidson

Publication

Shaner et al Nat Methods. 2008 Jun;5(6):545-51. doi:

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

LAMP1

Tags

mOrange2

Expression

Mammalian

Promoter

CMV

Available since

Oct. 24, 2014

Availability

Academic Institutions and Nonprofits only

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pet26-MBP-ELIC

Plasmid

#39239

Depositor

Raimund Dutzler

Publication

Hilf et al Nature. 2009 Jan 1. 457(7225):115-8.

Insert

Eriwinia chrysanthemi - pentameric ligand gated ion channel

Tags

His-10 and MBP

Expression

Bacterial

Promoter

T7

Available since

Aug. 28, 2012

Availability

Academic Institutions and Nonprofits only

Cited by

5 Articles

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pDEST-cDNA5-FRT/TO-3*Flag-APEX2 C-term

Plasmid

#182926

Purpose

Express FLAG epitope and APEX2-tagged fusion protein in mammalian cells.

Depositor

Benjamin Blencowe

Publication

Barutcu et al Mol Cell. 2022 Mar 3;82(5):1035-1052.e9.

Type

Empty backbone

Tags

APEX2 and FLAG

Expression

Mammalian

Promoter

CMV

Available since

July 28, 2022

Availability

Academic Institutions and Nonprofits only

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pCMV-GABRA1-IRES2-EGFP

Plasmid

#170820

Purpose

human GABAA receptors alpha1 subunit and IRES2-EGFP

Depositor

Tingwei Mu

Publication

Fu et al PLoS One. 2018 Nov 27;13(11):e0207948. d

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

Human GABAA receptor alpha1 subunit (GABRA1 Human)

Tags

IRES2-EGFP

Expression

Mammalian

Available since

Aug. 10, 2021

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-HA-RAD52-WT

Plasmid

#235676

Purpose

Expresses HA-tagged wild type human RAD52 in mammalian cells.

Depositor

Simon Powell

Publication

Jalan et al Nat Commun. 2024 Sep 5;15(1):7776. doi:

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

RAD52 (RAD52 Human)

Tags

HA

Expression

Mammalian

Mutation

wild type

Available since

April 8, 2025

Availability

Academic Institutions and Nonprofits only

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pCOLA-Gent-EM7-Erv1p-DsbC

Plasmid

#202485

Purpose

Expresses sulfhydryl oxidase (Erv1p) and disulfide bond isomerase (dsbC) constitutively

Depositor

Michael Lynch

Publication

Hennigan et al Metab Eng. 2024 Sep;85:116-130. doi: 10.

Insert

sulfhydryl oxidase (Erv1p) and disulfide bond isomerase (dsbC) (ERV1 Escherichia coli, Budding Yeast)

Expression

Bacterial

Promoter

EM7

Available since

Nov. 14, 2024

Availability

Academic Institutions and Nonprofits only

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Stx5L-mCherry

Plasmid

#209844

Purpose

Expresses Stx5L-mCherry in mammalian cells

Depositor

Geert van den Bogaart

Publication

Linders et al Nat Commun. 2021 Oct 28;12(1):6227. doi:

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

STX5 (STX5 Human)

Tags

mCherry

Expression

Mammalian

Mutation

Wildtype Stx5 (with 55M)

Promoter

CMV

Available since

Nov. 8, 2023

Availability

Academic Institutions and Nonprofits only

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pRDA_888

Plasmid

#201165

Purpose

Lentiviral expression of EnAsCas12a CRISPRa gRNA with N' nanobody-ALFA and C' p65, HSF1 and NLS

Depositor

John Doench

Publication

Griffith et al Cell Genom. 2023 Sep 1;3(9):100387. doi:

Insert

Puromycin resistance

Use

CRISPR and Lentiviral

Promoter

EFS

Available since

June 14, 2023

Availability

Academic Institutions and Nonprofits only

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pET-6xHis-GFP(+30)

Plasmid

#199164

Purpose

used to express a supercharged GFP variant in E. coli

Depositor

Allie Obermeyer

Publication

Yeong et al ACS Cent Sci. 2020 Dec 23;6(12):2301-231

Insert

GFP(+30)

Tags

MGHHHHHHGGA

Mutation

E16R, V21K, D29K, E42K, E44K, D86K, E100K, D112K,…

Promoter

T7

Available since

May 10, 2023

Availability

Academic Institutions and Nonprofits only

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pRelA*

Plasmid

#175590

Purpose

Tet inducible, KAN Resistant, fluor labelled (YFP) synthesis domain of the E. coli relA gene on a low copy backbone. Used to induce controllable synthesis of ppGpp in E. coli.

Depositor

Greg Bokinsky

Publication

Buke et al Curr Biol. 2022 Feb 28;32(4):870-877.e5.

Insert

Catalytic domain of E.coli relA gene.

Tags

Fused with a flexible GS linker to mVenus

Expression

Bacterial

Mutation

Only the catalytic domain of the enzyme is used (…

Available since

Nov. 19, 2021

Availability

Academic Institutions and Nonprofits only

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pGL3-Tet1 promoter A

Plasmid

#63881

Purpose

Luciferase reporter plasmid used to study transcriptional control of murine Tet1

Depositor

Kian Peng Koh

Publication

Sohni et al Mol Cell Biol. 2015 Mar 15;35(6):1026-42

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

Tet1 promoter fragment A

Use

Luciferase

Expression

Mammalian

Available since

May 19, 2015

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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sgRNA1_ASCL1

Plasmid

#64129

Purpose

Photoactivatable transcription system. Lentiviral expression of ASCL1 sgRNA1. Also contains a CMV-puro-t2A-mCherry expression cassette.

Depositor

Moritoshi Sato

Publication

Nihongaki et al Chem Biol. 2015 Feb 19;22(2):169-74. doi

Insert

sgRNA1_ASCL1 (ASCL1 Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

U6

Available since

April 15, 2015

Availability

Academic Institutions and Nonprofits only

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pFA6a-GST-kanMX6

Plasmid

#39293

Depositor

Jurg Bahler, John Pringle

Publication

Bahler et al Yeast. 1998 Jul;14(10):943-51.

Insert

KanR

Use

Yeast genomic targeting

Tags

A. gossypii translation elongation factor 1a gene…

Available since

Aug. 20, 2012

Availability

Academic Institutions and Nonprofits only

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pcDNA-FLAG-GPD1

Plasmid

#32487

Depositor

Pere Puigserver

Publication

Kelly et al Mol Cell Biol. 2011 Jul;31(13):2696-706.

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

GPD1 (Gpd1 Mouse)

Tags

FLAG

Expression

Mammalian

Promoter

CMV

Available since

Sept. 28, 2011

Availability

Academic Institutions and Nonprofits only
pMIR-CDH1 mutUTR

Plasmid

#25039

Depositor

Bob Weinberg

Publication

Ma et al Nat Cell Biol. 2010 Mar . 12(3):247-56.

Insert

CDH1 mutant 3'UTR (CDH1 Human)

Use

Luciferase

Expression

Mammalian

Mutation

substitution of 6nt within the miR-9 binding site…

Available since

June 7, 2010

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article
TLCV2-ApaLI*-FLAG-T2A-mTagBFP2-Puro

Plasmid

#211463

Purpose

Lentivirus backbone expressing a doxycycline-inducible mitochondrial localized ApaLI mutant (D211A, E225A and K227A)

Depositor

Agnel Sfeir

Publication

Fu et al Mol Cell. 2023 Oct 8:S1097-2765(23)00753

Insert

ApaLI-dead

Use

Lentiviral

Tags

FLAG

Expression

Mammalian

Mutation

D211A, E225A, K227A

Promoter

TRE

Available since

Dec. 21, 2023

Availability

Academic Institutions and Nonprofits only

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M249-5'csr-1-Pgsy-1-luciferase-Bar-3'csr-1

Plasmid

#89693

Purpose

Luciferase reporter plasmid targeted at csr-1 locus

Depositor

Christian Hong

Publication

Matsu-Ura et al Fungal Biol Biotechnol. 2015 Jul 15;2:4.

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

gsy-1 promoter, luciferase

Use

Yeast episomal vector

Tags

luciferase

Promoter

gsy-1

Available since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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