We narrowed to 82,304 results for: ELL

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pLX304-HsTtyh1-TwinStrep-His

Plasmid

#211332

Purpose

Mammalian overexpression vector for C-terminally TwinStrep and His tagged human Ttyh1 (W417R)

Depositor

Luke Chao

Article

Bell et al Elife. 2024 Aug 13;13:e100061. doi: 10.7

Insert

Tweety homology protein 1

Use

Lentiviral

Tags

TwinStrep, 10xHis

Expression

Mammalian

Mutation

W417R

Promoter

CMV

Available Since

July 22, 2024

Availability

Academic Institutions and Nonprofits only

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pRetroX-TRE3G-Tir

Plasmid

#221326

Purpose

Dox-inducible expression of Tir in mammalian cells by retroviral transduction

Depositor

Jonathan Kagan

Article

Landau et al Mol Cell. 2024 Jul 11;84(13):2436-2454.e

Insert

Tir

Use

Retroviral

Tags

HN-FLAG

Promoter

TRE3GV

Available Since

July 16, 2024

Availability

Academic Institutions and Nonprofits only

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pRetroX-TRE3G-Gag

Plasmid

#221325

Purpose

Dox-inducible expression of Gag in mammalian cells by retroviral transduction

Depositor

Jonathan Kagan

Article

Landau et al Mol Cell. 2024 Jul 11;84(13):2436-2454.e

Insert

Gag

Use

Retroviral

Tags

FLAG

Promoter

TRE3GV

Available Since

July 16, 2024

Availability

Academic Institutions and Nonprofits only

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pgRNA-bacteria_speG_M15

Plasmid

#220488

Purpose

inducible CRISPRi

Depositor

Emily Balskus

Article

Mayers et al Cell. 2024 Jun 11:S0092-8674(24)00579-8.

Insert

gspeG

Use

CRISPR and RNAi

Mutation

none

Available Since

June 26, 2024

Availability

Academic Institutions and Nonprofits only

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pgRNA-bacteria_speG_E23

Plasmid

#220489

Purpose

inducible CRISPRi

Depositor

Emily Balskus

Article

Mayers et al Cell. 2024 Jun 11:S0092-8674(24)00579-8.

Insert

gspeG

Use

CRISPR and RNAi

Mutation

none

Available Since

June 26, 2024

Availability

Academic Institutions and Nonprofits only

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pTrcHis_VectorA_short_speG_genomic

Plasmid

#220473

Purpose

heterologous expression under endogenous speG E. coli promoter

Depositor

Emily Balskus

Article

Mayers et al Cell. 2024 Jun 11:S0092-8674(24)00579-8.

Insert

speG

Expression

Bacterial

Mutation

none

Available Since

June 26, 2024

Availability

Academic Institutions and Nonprofits only

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pTrcHis_VectorA_short_speGpromoter_PA4114

Plasmid

#220474

Purpose

heterologous expression of PA4114 under endogenous speG E. coli promoter

Depositor

Emily Balskus

Article

Mayers et al Cell. 2024 Jun 11:S0092-8674(24)00579-8.

Insert

PA4114

Expression

Bacterial

Mutation

none

Available Since

June 26, 2024

Availability

Academic Institutions and Nonprofits only

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pTrcHis_VectorA_short_speGpromoter_PA1472

Plasmid

#220475

Purpose

heterologous expression of PA1472 under endogenous speG E. coli promoter

Depositor

Emily Balskus

Article

Mayers et al Cell. 2024 Jun 11:S0092-8674(24)00579-8.

Insert

PA1472

Expression

Bacterial

Mutation

none

Available Since

June 26, 2024

Availability

Academic Institutions and Nonprofits only

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pTrcHis_VectorA_short_speGpromoter_PA1377

Plasmid

#220476

Purpose

heterologous expression of PA1377 under endogenous speG E. coli promoter

Depositor

Emily Balskus

Article

Mayers et al Cell. 2024 Jun 11:S0092-8674(24)00579-8.

Insert

PA1377

Expression

Bacterial

Mutation

none

Available Since

June 26, 2024

Availability

Academic Institutions and Nonprofits only

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pMAT_41BBICD

Plasmid

#197097

Purpose

This plasmid contains the coding sequence for the intracellular domain of 4-1BB. Digest this insert and add it to pHR_pSFFV_scFVCD19_CD8a_CD3zP2AEGFP

Depositor

Wendell Lim

Article

Daniels et al Science. 2022 Dec 16;378(6625):1194-1200

Insert

This plasmid contains the coding sequence for the intracellular domain of 4-1BB.

Expression

Mammalian

Available Since

April 3, 2024

Availability

Academic Institutions and Nonprofits only

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pMAT_CD28ICD

Plasmid

#197098

Purpose

This plasmid contains the coding sequence for the intracellular domain of CD28. Digest this insert and add it to pHR_pSFFV_scFVCD19_CD8a_CD3zP2AEGFP

Depositor

Wendell Lim

Article

Daniels et al Science. 2022 Dec 16;378(6625):1194-1200

Insert

This plasmid contains the coding sequence for the intracellular domain of CD28.

Expression

Mammalian

Available Since

April 3, 2024

Availability

Academic Institutions and Nonprofits only

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pCF823_EFS-Cas9-P2A-Puro

Plasmid

#211645

Purpose

EFS-Cas9-P2A-Puro

Depositor

Christof Fellmann

Article

Tan et al Cell Rep. 2023 Nov 28;42(11):113339. doi

Insert

Cas9

Use

CRISPR and Lentiviral

Expression

Mammalian

Available Since

Feb. 1, 2024

Availability

Academic Institutions and Nonprofits only

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pCF226_EFS-Cas9-P2A-Puro

Plasmid

#211644

Purpose

EFS-Cas9-P2A-Puro

Depositor

Christof Fellmann

Article

Tan et al Cell Rep. 2023 Nov 28;42(11):113339. doi

Insert

Cas9

Use

CRISPR and Lentiviral

Expression

Mammalian

Available Since

Feb. 1, 2024

Availability

Academic Institutions and Nonprofits only

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pFH2.13_GagZip-MCP_Zip-P2A-eGFP

Plasmid

#205528

Purpose

Express HIV GagZip-MCP_Zip-P2A-eGFP

Depositor

Michael Elowitz

Article

Horns et al Cell. 2023 Jul 10:S0092-8674(23)00689-X.

Insert

GagZip-MCP_Zip

Expression

Mammalian

Mutation

Nucleocapsid replaced with leucine zipper

Promoter

CMV

Available Since

Sept. 26, 2023

Availability

Academic Institutions and Nonprofits only

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pFH2.7_Gag-dZF2-MCP-P2A-eGFP

Plasmid

#205523

Purpose

Express HIV Gag-delta-ZF2-MCP-P2A-eGFP

Depositor

Michael Elowitz

Article

Horns et al Cell. 2023 Jul 10:S0092-8674(23)00689-X.

Insert

Gag delta ZF2-MCP

Expression

Mammalian

Mutation

ZF2 deleted

Promoter

CMV

Available Since

Sept. 26, 2023

Availability

Academic Institutions and Nonprofits only

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pFH2.14_GagZip-dp1dp6-MCP-P2A-eGFP

Plasmid

#205529

Purpose

Express HIV GagZip-dp1dp6-MCP-P2A-eGFP

Depositor

Michael Elowitz

Article

Horns et al Cell. 2023 Jul 10:S0092-8674(23)00689-X.

Insert

GagZip-dp1dp6-MCP

Expression

Mammalian

Mutation

Nucleocapsid replaced with leucine zipper, p1 and…

Promoter

CMV

Available Since

Sept. 26, 2023

Availability

Academic Institutions and Nonprofits only

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pFH2.99_PB-EPN1-MCP_I-T2A-eGFP-PGK-Blast

Plasmid

#205544

Purpose

Express EPN1-MCP-I-T2A-eGFP and blasticidin resistance marker

Depositor

Michael Elowitz

Article

Horns et al Cell. 2023 Jul 10:S0092-8674(23)00689-X.

Insert

EPN1-MCP-I

Use

Piggybac

Expression

Mammalian

Mutation

WT

Promoter

EF1

Available Since

Sept. 19, 2023

Availability

Academic Institutions and Nonprofits only

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XLone-GvpB

Plasmid

#173798

Purpose

Mammalian acoustic reporter gene 1 cassette 1 (expressing GvpB)

Depositor

Xiaojun Lian

Article

Howells et al Bioeng Transl Med. 2023 Aug 2;9(2):e1058

Insert

GvpB

Tags

mCherry (K187N -please see depositor comments)

Expression

Mammalian

Promoter

TRE3G promoter

Available Since

July 14, 2023

Availability

Academic Institutions and Nonprofits only

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pPT-PCR C1

Plasmid

#141248

Purpose

PCR cloned Phaeodactylum tricornutum Mitochondrial Genome - Clone 1

Depositor

Bogumil Karas

Article

Cochrane et al Algal Research, 2020. 50, 102014

Insert

Reduced Mitochondrial Genome of Phaeodactylum tricornutum

Use

Synthetic Biology; Diatom expression

Expression

Bacterial and Yeast

Mutation

Repeat region removed, additional minor mutations…

Available Since

May 17, 2023

Availability

Academic Institutions and Nonprofits only

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LT2_32_PyruEth_X2site

Plasmid

#194428

Purpose

Plasmid for genome integration in X2 site expressing PyruEth (Pyruvate and Ethanol consumption sensors)

Depositor

Lisbeth Olsson

Article

Torello Pianale et al ACS Synth Biol. 2023 Aug 8. doi: 10.1021

Insert

PyruEth

Use

Used as donor dna in genome integration after lin…

Expression

Yeast

Mutation

NA

Available Since

May 8, 2023

Availability

Academic Institutions and Nonprofits only

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LT1_75_Donor_Tag_RPL13A-mTurq2

Plasmid

#194415

Purpose

Plasmid for genome integration of mTurquoise2 CDS to tag native RPL13A for biosensors RibPro and UPRpro

Depositor

Lisbeth Olsson

Article

Torello Pianale et al ACS Synth Biol. 2023 Aug 8. doi: 10.1021

Insert

mTurquoise2 CDS

Use

Synthetic Biology; Used as donor dna in genome in…

Expression

Yeast

Mutation

NA

Available Since

May 8, 2023

Availability

Academic Institutions and Nonprofits only

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pHR_PGK_SNIPR_FAP site

Plasmid

#188350

Purpose

Lentiviral vector - constitutive expression of an myctag SNIPR with a FAPsite, a Notch1-TMD, a Notch1-JMD, and a Gal4VP64 transcriptional factor

Depositor

Kole Roybal

Article

Zhu et al Cell. 2022 Apr 14;185(8):1431-1443.e16.

Insert

PGK_myctag_FAPsite_Notch1-TMD_Notch1-JMD_Gal4VP64

Use

Lentiviral

Available Since

March 9, 2023

Availability

Academic Institutions and Nonprofits only

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pXD70Tet(DadRmt1)-DadR(ΔDBD)

Plasmid

#191637

Purpose

E. coli - Eggerthella lenta shuttle plasmid (TetR), dadR(ΔDBD) complementation

Depositor

Emily Balskus

Article

Dong et al Nat Commun. 2022 Dec 9;13(1):7624. doi:

Insert

DadR

Expression

Bacterial

Mutation

ΔDBD

Available Since

Jan. 5, 2023

Availability

Academic Institutions and Nonprofits only

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pXD70Tet(DadRmt2)-DadR(ΔTM)

Plasmid

#191638

Purpose

E. coli - Eggerthella lenta shuttle plasmid (TetR), dadR(ΔTM) complementation

Depositor

Emily Balskus

Article

Dong et al Nat Commun. 2022 Dec 9;13(1):7624. doi:

Insert

DadR

Expression

Bacterial

Mutation

ΔTM

Available Since

Jan. 5, 2023

Availability

Academic Institutions and Nonprofits only

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pXD70Tet(HcdR)-HcdR

Plasmid

#191648

Purpose

E. coli - Eggerthella lenta shuttle plasmid (TetR), hcdR complementation

Depositor

Emily Balskus

Article

Dong et al Nat Commun. 2022 Dec 9;13(1):7624. doi:

Insert

HcdR

Expression

Bacterial

Available Since

Jan. 5, 2023

Availability

Academic Institutions and Nonprofits only

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pXD70Tet(CadR)-CadR

Plasmid

#191649

Purpose

E. coli - Eggerthella lenta shuttle plasmid (TetR), cadR complementation

Depositor

Emily Balskus

Article

Dong et al Nat Commun. 2022 Dec 9;13(1):7624. doi:

Insert

CadR

Expression

Bacterial

Available Since

Jan. 5, 2023

Availability

Academic Institutions and Nonprofits only

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pAAV CMV CCR6-eLOV-TEVcs-Gal4

Plasmid

#173113

Purpose

Expresses CCR6 receiver construct in mammalian cells

Depositor

Alice Ting

Article

Cho et al Elife. 2022 Mar 21;11:e70881. doi: 10.75

Insert

CCR6-eLOV-TEVcs-tTA

Use

AAV

Promoter

CMV

Available Since

Dec. 21, 2022

Availability

Academic Institutions and Nonprofits only

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UAS-GFP::NMN-Deamidase

Plasmid

#187868

Purpose

Gal4/UAS expression of GFP::NMN-Deamidase

Depositor

Lukas Neukomm

Article

Llobet Rosell et al Elife. 2022 Dec 7;11:e80245. doi: 10.755

Insert

NMN-Deamidase

Tags

GFP

Expression

Insect

Available Since

Dec. 12, 2022

Availability

Academic Institutions and Nonprofits only

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EGFP-Myo1b motor

Plasmid

#187364

Purpose

Expresses rat myosin 1b isoform b motor domain labelled with EGFP

Depositor

Evelyne Coudrier

Article

Prosperi et al J Cell Biol. 2015 Jul 20;210(2):347-61.

Insert

myosin 1b isoform b motor domain (Myo1b Rat)

Tags

EGFP

Expression

Mammalian

Promoter

CMV

Available Since

Aug. 22, 2022

Availability

Academic Institutions and Nonprofits only

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EGFP-Plekhh1-MyTH-FERM

Plasmid

#187370

Purpose

Expresses human Plekhh1-MyTH-FERM labelled with EGFP

Depositor

Evelyne Coudrier

Article

Prosperi et al J Cell Sci. 2021 Dec 1;134(23). pii: 272

Insert

Plekhh1-MyTH-FERM

Tags

EGFP

Expression

Mammalian

Promoter

CMV

Available Since

Aug. 5, 2022

Availability

Academic Institutions and Nonprofits only

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EGFP-Myo1b-Tail

Plasmid

#187365

Purpose

Expresses rat myosin 1b isoform b Tail domain labelled with EGFP

Depositor

Evelyne Coudrier

Article

Prosperi et al J Cell Biol. 2015 Jul 20;210(2):347-61.

Insert

myosin 1b isoform b Tail domain (Myo1b Rat)

Tags

EGFP

Expression

Mammalian

Promoter

CMV

Available Since

Aug. 5, 2022

Availability

Academic Institutions and Nonprofits only

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TOMM20-mTurquoise-RhoG-QL-deltaCaaX

Plasmid

#176122

Purpose

Constitutively active, mitochondiral localized RhoG

Depositor

Dorus Gadella

Article

Mahlandt et al J Cell Sci. 2023 May 15;136(10):jcs26080

Insert

RHOG (RHOG Human)

Expression

Mammalian

Mutation

QL

Available Since

Oct. 29, 2021

Availability

Academic Institutions and Nonprofits only

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pRS426-CeLACE-1

Plasmid

#174523

Purpose

Express C.elegans LACE-1 in yeast cells

Depositor

Oleh Khalimonchuk

Article

Germany et al J Cell Sci. 2018 Nov 21;131(22). pii: jc

Insert

lace-1 (C30F12.2 Nematode)

Tags

6xHis

Expression

Yeast

Promoter

MET25

Available Since

Sept. 16, 2021

Availability

Academic Institutions and Nonprofits only

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Bax gRNA#1

Plasmid

#171827

Purpose

Cas9-mediated knockout of Bax in mammalian cells

Depositor

Takeshi Imai

Article

Aihara et al Cell Rep. 2021 Jun 22;35(12):109276. doi

Insert

Bax (Bax Mouse)

Use

CRISPR

Expression

Mammalian

Available Since

Aug. 25, 2021

Availability

Academic Institutions and Nonprofits only

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Bax gRNA#2

Plasmid

#171828

Purpose

Cas9-mediated knockout of Bax in mammalian cells

Depositor

Takeshi Imai

Article

Aihara et al Cell Rep. 2021 Jun 22;35(12):109276. doi

Insert

Bax (Bax Mouse)

Use

CRISPR

Expression

Mammalian

Available Since

Aug. 25, 2021

Availability

Academic Institutions and Nonprofits only

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Bax gRNA#3

Plasmid

#171829

Purpose

Cas9-mediated knockout of Bax in mammalian cells

Depositor

Takeshi Imai

Article

Aihara et al Cell Rep. 2021 Jun 22;35(12):109276. doi

Insert

Bax (Bax Mouse)

Use

CRISPR

Expression

Mammalian

Available Since

Aug. 25, 2021

Availability

Academic Institutions and Nonprofits only

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pCAFNF-Cdc42

Plasmid

#163606

Purpose

Expresses Cdc42 Flp-dependently in mammalian cells

Depositor

Takeshi Imai

Article

Aihara et al Cell Rep. 2021 Jun 22;35(12):109276. doi

Insert

Cdc42 (Cdc42 Mouse)

Expression

Mammalian

Available Since

Aug. 23, 2021

Availability

Academic Institutions and Nonprofits only

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pPB-tetpA(R1R4)-iRPT

Plasmid

#154882

Purpose

Ubiquitously expresses iRFP713, Puromycin resistance gene, and rtTAM2.

Depositor

Hideyuki Okano

Article

Nemoto et al Cells. 2020 Dec 22;10(1). pii: cells1001

Inserts

iRFP713
Puromycin resistance gene
rtTAM2

Expression

Mammalian

Available Since

Jan. 5, 2021

Availability

Academic Institutions and Nonprofits only

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pKM54

Plasmid

#154157

Purpose

Firefly luciferase under the control of 8 copies of the Aedes aegypti Rel2 NF-κB site

Depositor

Kevin Maringer

Article

Russell et al PLoS Negl Trop Dis. 2021 Feb 16;15(2):e0

Insert

Firefly luciferase

Use

Luciferase

Promoter

8 copies of the Aedes aegypti Rel2 NF-κB site

Available Since

Aug. 21, 2020

Availability

Academic Institutions and Nonprofits only

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pKM44

Plasmid

#154153

Purpose

Firefly luciferase under the control of the Drosophila melanogaster minimal Hsp70 promoter

Depositor

Kevin Maringer

Article

Russell et al PLoS Negl Trop Dis. 2021 Feb 16;15(2):e0

Insert

Firefly luciferase

Use

Luciferase

Promoter

Drosophila melanogaster minimal Hsp70 promoter

Available Since

Aug. 21, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge

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We narrowed to 82,304 results for: ELL