We narrowed to 44,104 results for: cha
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Plasmid#195702PurposeLentiviral plasmid encoding SYT9 with D330, 332N mutations followed by an internal ribosomal entry site followed by EGFP under the human synapsin promoterDepositorInsertSyt9 (Syt5 Mouse)
UseLentiviralTagsExpressionMutationfull-length mouse SYT9 with D330, 332N mutationsPromoterAvailable SinceOct. 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
cacna2d1 BBS pMT2
Plasmid#206062Purposeexpression of rat calcium channel alpha2delta-1 with a single Bungarotoxin Binding Site motif inserted after aa549DepositorAvailable SinceSept. 14, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCENPBR pc861
Plasmid#167569PurposeHuman CENP-B (aa 1-169 containing DNA-binding domain) cloned into pDsRed1-N1 vector (Clontech).DepositorInsertHomo sapiens centromere protein B (CENPB Human)
UseTagsDs-RedExpressionMammalianMutationPromoterCMVAvailable SinceJune 14, 2023AvailabilityAcademic Institutions and Nonprofits only -
pT7-EGFP-C1-HsDCP1a-H19A_V
Plasmid#147799PurposeMammalian Expression of HsDCP1a-H19ADepositorInsertHsDCP1a-H19A (DCP1A Human)
UseTagsExpressionMammalianMutationtwo silent mutations T237C and T1716G compared to…PromoterAvailable SinceMarch 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
pT7-EGFP-C1-HsDCP1a-N74G_V
Plasmid#147800PurposeMammalian Expression of HsDCP1a-N74GDepositorInsertHsDCP1a-N74G (DCP1A Human)
UseTagsExpressionMammalianMutationtwo silent mutations T237C and T1716G compared to…PromoterAvailable SinceMarch 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
pT7-EGFP-C1-HsDCP1a-Q18AH19A_V
Plasmid#147801PurposeMammalian Expression of HsDCP1a-Q18AH19ADepositorInsertHsDCP1a-Q18AH19A (DCP1A Human)
UseTagsExpressionMammalianMutationtwo silent mutations T237C and T1716G compared to…PromoterAvailable SinceMarch 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
pT7-EGFP-C1-HsDCP1a-R72G_V
Plasmid#147802PurposeMammalian Expression of HsDCP1a-R72GDepositorInsertHsDCP1a-R72G (DCP1A Human)
UseTagsExpressionMammalianMutationtwo silent mutations T237C and T1716G compared to…PromoterAvailable SinceMarch 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
pT7-EGFP-C1-HsDCP1a-R72GN74G_V
Plasmid#147803PurposeMammalian Expression of HsDCP1a-R72GN74GDepositorInsertHsDCP1a-R72GN74G (DCP1A Human)
UseTagsExpressionMammalianMutationtwo silent mutations T237C and T1716G compared to…PromoterAvailable SinceMarch 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCIneo-lambdaN-HA-HsDCP2_1-244_V
Plasmid#147790PurposeMammalian Expression of HsDCP2_1-244DepositorInsertHsDCP2_1-244 (DCP2 Human)
UseTagsExpressionMammalianMutationone silent mutation A429G compared to the sequenc…PromoterAvailable SinceMarch 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
pRSFDuetG-HsEDC4_977-1401-HsDCP2_395-420_K
Plasmid#146809PurposeBacterial Expression of HsEDC4_977-1401-HsDCP2_385-420DepositorAvailable SinceMarch 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
pMeCP2miRFP670 pc3942
Plasmid#167568PurposeThe miRFP670-gene is ligated c-terminally to the rat meCP2-gene.DepositorAvailable SinceMarch 8, 2023AvailabilityAcademic Institutions and Nonprofits only -
pEE059
Plasmid#176816PurposeEncodes codon optimized putative thermophilic PET hydrolase enzyme for bacterial expressionDepositorInsert610
UseTags6xHisExpressionBacterialMutationPromoterT7Available SinceNov. 28, 2022AvailabilityAcademic Institutions and Nonprofits only -
pT7-EGFP-C1-HsDCP2-E148QF404AF407AF409A_V
Plasmid#147812PurposeMammalian Expression of HsDCP2-E148QF404AF407AF409ADepositorInsertHsDCP2-E148QF404AF407AF409A (DCP2 Human)
UseTagsExpressionMammalianMutationtwo silent mutations compared to the sequence giv…PromoterAvailable SinceNov. 1, 2022AvailabilityAcademic Institutions and Nonprofits only -
pT7-EGFP-C1-HsDCP1a-S12AH19A_V
Plasmid#147804PurposeMammalian Expression of HsDCP1a-S12AH19ADepositorInsertHsDCP1a-S12AH19A (DCP1A Human)
UseTagsExpressionMammalianMutationtwo silent mutations T237C and T1716G compared to…PromoterAvailable SinceNov. 1, 2022AvailabilityAcademic Institutions and Nonprofits only -
pT7-EGFP-C1-HsDCP2-5xmut_V
Plasmid#147806PurposeMammalian Expression of HsDCP2-5xmutDepositorInsertHsDCP2-5xmut (DCP2 Human)
UseTagsExpressionMammalianMutationtwo silent mutations compared to the sequence gi…PromoterAvailable SinceNov. 1, 2022AvailabilityAcademic Institutions and Nonprofits only -
pT7-EGFP-C1-HsDCP2_97-420_V
Plasmid#147810PurposeMammalian Expression of HsDCP2_97-420DepositorInsertHsDCP2_97-420 (DCP2 Human)
UseTagsExpressionMammalianMutationone silent mutation compared to the sequence give…PromoterAvailable SinceNov. 1, 2022AvailabilityAcademic Institutions and Nonprofits only -
pT7-EGFP-C1-HsDCP2-D15AR19A_V
Plasmid#147811PurposeMammalian Expression of HsDCP2-D15AR19ADepositorInsertHsDCP2-D15AR19A (DCP2 Human)
UseTagsExpressionMammalianMutationTwo silent mutations compared to the sequence giv…PromoterAvailable SinceNov. 1, 2022AvailabilityAcademic Institutions and Nonprofits only -
ACE2-IgFc probe
Plasmid#184787PurposeExpression of His-tagged, soluble ACE2-IgFc microbodyDepositorInserthACE2-IgFc (ACE2 Synthetic)
UseTags6xHis TagExpressionMammalianMutationPromoterEF1-alphaAvailable SinceOct. 19, 2022AvailabilityAcademic Institutions and Nonprofits only -
pT7-EGFP-C1-HsDCP1a-S12AQ18AH19A_V
Plasmid#147805PurposeMammalian Expression of HsDCP1a-S12AQ18AH19ADepositorInsertHsDCP1a-S12AQ18AH19A (DCP1A Human)
UseTagsExpressionMammalianMutationtwo silent mutations T237C and T1716G compared to…PromoterAvailable SinceMarch 23, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCSJ982
Plasmid#176622PurposeExpress yeast Ub-MPGLW-Fbp1-3xflag and reference flag-DHFR-ha protein, both are contolled by tetracycline mediated TDH promter, test protein expression and stability.DepositorInsertUb-MPGLW-Fbp1
UseTagsUbiquitin N-terminal fusion and triple flag tag C…ExpressionYeastMutationChanged first 4 N-terminal amino acid residues of…PromoterTDH3tc3Available SinceMarch 10, 2022AvailabilityAcademic Institutions and Nonprofits only -
pGEX-4T Doc2beta C2AB(125) H158C
Plasmid#134691PurposeEncodes cytoplasmic domain of Doc2beta for bacterial expression, purification, and labelingDepositorInsertDouble C2 protein beta (Doc2b Rat)
UseTagsGSTExpressionBacterialMutationH158C, C145A, C217A, C249A, C290A, C338A, C387APromoterAvailable SinceNov. 8, 2021AvailabilityAcademic Institutions and Nonprofits only -
pGEX-4T Doc2beta C2AB(125) F222C
Plasmid#134692PurposeEncodes cytoplasmic domain of Doc2beta for bacterial expression, purification, and labelingDepositorInsertDouble C2 protein beta (Doc2b Rat)
UseTagsGSTExpressionBacterialMutationF222C, C145A, C217A, C249A, C290A, C338A, C387APromoterAvailable SinceNov. 8, 2021AvailabilityAcademic Institutions and Nonprofits only -
pGEX-4T Doc2beta C2AB(125) A298C
Plasmid#134693PurposeEncodes cytoplasmic domain of Doc2beta for bacterial expression, purification, and labelingDepositorInsertDouble C2 protein beta (Doc2b Rat)
UseTagsGSTExpressionBacterialMutationA298C, C145A, C217A, C249A, C290A, C338A, C387APromoterAvailable SinceNov. 8, 2021AvailabilityAcademic Institutions and Nonprofits only -
NmMetQ C20A
Plasmid#172112PurposeExpression of Methionine Binding Protein (MetQ) with C20A mutation in signal sequenceDepositorInsertMethionine Binding Protein
UseTags10x Histidine Tag and N.meningitidis signal seque…ExpressionBacterialMutationChanged Proline 22 to Alanine on MetQ and Cystein…PromoterT7 PromoterAvailable SinceOct. 29, 2021AvailabilityAcademic Institutions and Nonprofits only -
FKBP-EGFP-RanGAP*
Plasmid#175238PurposeBacterial expression and purification, low affinity SUMOylation substrate that can recruited to FRB with rapamycin, point mutation F562A reduces affinity for E2, increasing the KmDepositorInsertRANGAP1 (RANGAP1 Human)
UseTagsExpressionBacterialMutationAmino acid 562 F to APromotertacAvailable SinceOct. 18, 2021AvailabilityAcademic Institutions and Nonprofits only -
pAAV-EF1α1.1-FLEX-rc [ChromeQ-GFP]
Plasmid#153542PurposeAAV-mediated expression of ChromeQ-GFP under the EF1α promoter (1.1kb short version), in floxed/reversed (Cre-dependent) manner.DepositorInsertChromeQ-GFP
UseAAVTagsGFPExpressionMammalianMutationPromoterEF1α1.1Available SinceAug. 3, 2021AvailabilityAcademic Institutions and Nonprofits only -
pAAV-EF1α1.1-FLEX-rc [ChromeT-GFP]
Plasmid#153541PurposeAAV-mediated expression of ChromeT-GFP under the EF1α promoter (1.1kb short version), in floxed/reversed (Cre-dependent) manner.DepositorInsertChromeT-GFP
UseAAVTagsGFPExpressionMammalianMutationPromoterEF1α1.1Available SinceAug. 3, 2021AvailabilityAcademic Institutions and Nonprofits only -
XLone-Puro Cas13d-eGFP U6 RUNX1 g1
Plasmid#155185PurposePiggybacTransposon-based tunable and temporal expression control of Cas13d- eGFP and RUNX1 gRNA1DepositorInsertCas13d RUNX1 gRNA1
UsePiggybac transposonTagsExpressionMammalianMutationPromoterU6Available SinceJune 24, 2021AvailabilityAcademic Institutions and Nonprofits only -
pGMC00007
Plasmid#166725Purposenon targeting sgRNADepositorInsertNon-targeting guide
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterAvailable SinceJune 9, 2021AvailabilityAcademic Institutions and Nonprofits only -
pAAV-Syn-FLEX-rc [ChromeT-GFP]
Plasmid#153539PurposeAAV-mediated expression of ChromeT-GFP under the Syn promoter, in floxed/reversed (Cre-dependent) manner.DepositorInsertChromeT-GFP
UseAAVTagsGFPExpressionMammalianMutationPromoterSynAvailable SinceFeb. 9, 2021AvailabilityAcademic Institutions and Nonprofits only -
EC71102
Plasmid#154065PurposeLevel 0 Golden Gate vector; Unmodifed CREDepositorInsertCRE
UseSynthetic Biology; Standard cloning vector used b…TagsExpressionMutationAll BsaI, Esp3I and BPiI restriction sites were r…PromoterAvailable SinceSept. 25, 2020AvailabilityAcademic Institutions and Nonprofits only -
pTCO2-mTDGi.2
Plasmid#149431PurposeminiTdg gene, SUMOylation mutantDepositorInsertTdg (Tdg Mouse)
UseCre/LoxTagsExpressionMammalianMutationK341A SUMOylation mutant, SNPs of Tdg gene in OLA…PromoterTdg promoterAvailable SinceSept. 10, 2020AvailabilityAcademic Institutions and Nonprofits only -
pTCO2-mTDGi.1
Plasmid#149430PurposeminiTdg gene, catalytic mutantDepositorInsertTdg (Tdg Mouse)
UseCre/LoxTagsExpressionMammalianMutationN151A catalytic mutant, SNPs of Tdg gene in OLA/1…PromoterTdg promoterAvailable SinceSept. 10, 2020AvailabilityAcademic Institutions and Nonprofits only -
LIC-Z-delCry2
Plasmid#153545Purposethis is the non-clustering version of LIC-Z tagged with mcherryDepositorInserthuman TCR ζ-Chain (CD247 Human)
UseTagsmCherryExpressionMammalianMutationwith Cry2 sequence deleted from LIC-ZPromoterCMVAvailable SinceJuly 17, 2020AvailabilityAcademic Institutions and Nonprofits only -
SNX30-PX (86-205)
Plasmid#119109PurposeBacterial expression of human phox homology (PX) domain, SNX30-PX (86-205)DepositorAvailable SinceMarch 30, 2020AvailabilityAcademic Institutions and Nonprofits only -
pcDNA-HisA-HOXC6-2
Plasmid#110365PurposeMammalian expression of HisA-tagged HOXC6-2DepositorAvailable SinceMarch 10, 2020AvailabilityAcademic Institutions and Nonprofits only -
pKdH_P-GAP-ScACS1*
Plasmid#126736Purposeyeast plasmid overexpressing ACS1 from Saccharomyces cerevisiaeDepositorInsertacs1*
UseTags6xHis,MycExpressionYeastMutationT3542C and A30S, P113S, and S629N (please see dep…PromoterGAPAvailable SinceJuly 31, 2019AvailabilityAcademic Institutions and Nonprofits only -
pKdH_P-GAP-ScADH2+P-GAP*-ScACS1*
Plasmid#126738Purposeyeast plasmid overexpressing ADH2 and ACS1 from Saccharomyces cerevisiaeDepositorInsertadh2,acs1*
UseTags6xHis,MycExpressionYeastMutationT3542CPromoterGAPAvailable SinceJuly 30, 2019AvailabilityAcademic Institutions and Nonprofits only -
CSII-IDR2-owlTRIMCyp-FOS K283D Q287D
Plasmid#79068PurposeExpress owl monkey TRIMCyp K283D, Q287D in mammalan cellsDepositorInsertTRIM5Cyp (owl monkey) (K283D, Q287D)-FOS
UseTagsOneSTrEP-FLAGExpressionMammalianMutationChanged Lys 283 to Asp and Gln 287 to AspPromoterCMVAvailable SinceJan. 30, 2017AvailabilityAcademic Institutions and Nonprofits only -
exon K-renilla
Plasmid#81007Purposeluciferase based mini-gene to report splicing at exon 25 in Drosophila paraylticDepositorInsertparalytic (para Fly)
UseTagsRenilla luciferaseExpressionInsectMutationspanning exon 24 to exon 26, termination codon in…PromoterP-AC5 (Actin 5C)Available SinceAug. 19, 2016AvailabilityAcademic Institutions and Nonprofits only -
pET-20b-T7-Ntx-K49
Plasmid#58715PurposeExpresses in Escherichia coli T7-Notexin with the amino acid substitution D49KDepositorInsertNotexin
UseTagsT7 peptide tagExpressionBacterialMutationChanged Aspartic Acid 49 to LysinePromoterT7Available SinceAug. 11, 2014AvailabilityAcademic Institutions and Nonprofits only -
pSC-mTDG-K341R-IntE2
Plasmid#52269PurposeBacterial expression of mouse TDG-K341R C-terminally fused to GyrA intein, SUMO-E2 and GSTDepositorInsertTDG (Tdg Mouse)
UseTagsGyrA-intein-Ubc9-GSTExpressionBacterialMutationK341R, SUMO acceptor site mutationPromoterT7Available SinceJuly 10, 2014AvailabilityAcademic Institutions and Nonprofits only -
Hoxd13-3'UTR
Plasmid#31525DepositorInsertHoxd13-3'UTR (Hoxd13 Mouse)
UseLuciferaseTagsLuciferaseExpressionMammalianMutationPromoterAvailable SinceOct. 4, 2011AvailabilityAcademic Institutions and Nonprofits only -
SNX9-PX (246-376)
Plasmid#119090PurposeBacterial expression of human phox homology (PX) domain, SNX9-PX (246-376)DepositorAvailable SinceAvailabilityAcademic Institutions and Nonprofits only -
PI3KC2gamma-PX (1200-1310)
Plasmid#119121PurposeBacterial expression of human phox homology (PX) domain, PI3KC2gamma-PX (1200-1310)DepositorInsertPI3KC2gamma-PX (1200-1310) (PIK3C2G Human)
UseTagsGSTExpressionBacterialMutationPromotertacAvailable SinceAvailabilityAcademic Institutions and Nonprofits only -
pFSW Doc2beta 6X mutant
Plasmid#128816PurposeTo make virus expressing Doc2beta 6X mutantDepositorInsertDoc2beta (Doc2b Rat)
UseLentiviralTagsIRES-EGFPExpressionMutationD163A, D218A, D220N, D303A, D357A, D359APromoterhSyn (human synapsin I promoter)Available SinceAvailabilityAcademic Institutions and Nonprofits only -
pLVX-EF1alpha-eGFP-2xStrep-IRES-Puro
Plasmid#141395PurposeLentiviral expression of SARS-CoV-2 protein; transient expression and generate lentivirusDepositorInserteGFP
UseLentiviralTags2xStrepExpressionMammalianMutationhuman codon optimizedPromoterEF1alphaAvailable SinceApril 13, 2020AvailabilityAcademic Institutions and Nonprofits only -
YFP NLS Beta-Actin
Plasmid#60613PurposeEncodes YFP and NLS tagged beta-actinDepositorAvailable SinceNov. 21, 2014AvailabilityAcademic Institutions and Nonprofits only -
PB-TO-ASCL1-DLX2
Plasmid#182307PurposePiggybac Tet-ON plasmid for differentiating hiPSCs into GABAergic neuron via ASCL1 and DLX2 expressionUseTagsNone and T2A-mycNLS-mTagBFP2ExpressionMammalianMutationPromoterEF1a and pTRE3G-bidirectionalAvailable SinceApril 14, 2022AvailabilityAcademic Institutions and Nonprofits only -
pcDNA-HA-ER WT
Plasmid#49498PurposeExpresses HA-tagged ER wild-type in mammalian cellsDepositorAvailable SinceDec. 12, 2013AvailabilityAcademic Institutions and Nonprofits only