We narrowed to 18,335 results for: met

1. pGAPDH::H2B-mScarlet

   Plasmid

   #186764

   Purpose

   A plasmid containing planarian H2B fused to a planarian codon-optimized mScarlet, driven by the planarian GAPDH promoter.

   Depositor

   Bo Wang

   Article

   Hall et al Cell Rep Methods. 2022 Sep 20;2(10):1002

   Insert

   H2B-mScarlet
   

   Use

   Overexpression

   Tags

   H2B

   Expression

   Mutation

   Promoter

   GAPDH

   Available Since

   Oct. 24, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pTriEx-mCherry-Zdk1-Rac1 Q61L

   Plasmid

   #81059

   Purpose

   mammalian expression of Zdk1 tagged Rac1 Q61L

   Depositor

   Klaus Hahn

   Article

   Wang et al Nat Methods. 2016 Jul 18. doi: 10.1038/n

   Inserts

   Zdk1
   Rac1 Q61L (RHOA Human)
   

   Use

   Tags

   mCherry

   Expression

   Bacterial, Insect, and Mamm…

   Mutation

   Promoter

   Available Since

   Aug. 30, 2016

   Availability

   Academic Institutions and Nonprofits only

3. pECE HA AMPKa1 AS

   Plasmid

   #69831

   Purpose

   Expresses Expresses AMPK alpha 1 in mammalian cells

   Depositor

   Anne Brunet

   Article

   Schaffer et al Cell Metab. 2015 Nov 3;22(5):907-21. doi

   Insert

   5'-AMP-activated protein kinase catalytic subunit alpha-1 (PRKAA1 Human)
   

   Use

   Tags

   HA

   Expression

   Mammalian

   Mutation

   Promoter

   SV40 early promoter

   Available Since

   May 12, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pTriEx-mCherry-Zdk1-RhoA Q63L

   Plasmid

   #81058

   Purpose

   mammalian expression of Zdk1 tagged RhoA Q63L

   Depositor

   Klaus Hahn

   Article

   Wang et al Nat Methods. 2016 Jul 18. doi: 10.1038/n

   Inserts

   Zdk1
   RhoA Q63L (RHOA Human)
   

   Use

   Tags

   mCherry

   Expression

   Bacterial, Insect, and Mamm…

   Mutation

   Promoter

   Available Since

   Aug. 30, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pTriEx-mVenus-Zdk1-RhoA Q63L

   Plasmid

   #81046

   Purpose

   mammalian expression of Zdk1 tagged RhoA Q63L

   Depositor

   Klaus Hahn

   Article

   Wang et al Nat Methods. 2016 Jul 18. doi: 10.1038/n

   Inserts

   Zdk1
   RhoA Q63L (RHOA Human)
   

   Use

   Tags

   mVenus

   Expression

   Bacterial, Insect, and Mamm…

   Mutation

   Promoter

   Available Since

   Aug. 16, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pLentiCRISPR-v1-sgGLS_6

   Plasmid

   #163460

   Purpose

   lentiviral vector expressing Cas9 and an sgRNA targeting GLS

   Depositor

   Jason Cantor

   Article

   Rossiter et al Cell Metab. 2021 Feb 23. pii: S1550-4131

   Insert

   sgRNA 6 targeting GLS (GLS Human)
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available Since

   Jan. 26, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pTriEx-mVenus-Zdk1-Rac1 Q61L

   Plasmid

   #81137

   Purpose

   mammalian expression of Zdk1 tagged Rac1 Q61L

   Depositor

   Klaus Hahn

   Article

   Wang et al Nat Methods. 2016 Jul 18. doi: 10.1038/n

   Inserts

   Zdk1
   Rac1 (RHOA Human)
   

   Use

   Tags

   mVenus

   Expression

   Bacterial, Insect, and Mamm…

   Mutation

   Q61L

   Promoter

   Available Since

   Aug. 29, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pLentiCRISPR-v1-sgGPT2_5

   Plasmid

   #163455

   Purpose

   lentiviral vector expressing Cas9 and an sgRNA targeting GPT2

   Depositor

   Jason Cantor

   Article

   Rossiter et al Cell Metab. 2021 Feb 23. pii: S1550-4131

   Insert

   sgRNA 5 targeting GPT2 (GPT2 Human)
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available Since

   Jan. 26, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pTag-RFP-C-h-Rab4a-c-Myc

   Plasmid

   #79800

   Purpose

   Express TagRFP labeled human Rab5a (GTPase located on recycling endosomal membranes).

   Depositor

   James Johnson

   Article

   Boothe et al Mol Metab. 2016 Feb 10;5(5):366-78. doi:

   Insert

   Rab4a (RAB4A Human)
   

   Use

   Tags

   TagRFP and myc

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   July 22, 2016

   Availability

   Industry, Academic Institutions, and Nonprofits

   

   Enlarge

10. pEB31 (hANGPTL8-V5-His lenti)

    Plasmid

    #109116

    Purpose

    V5 and His-tagged human ANGPTL8 in a lentiviral vector

    Depositor

    Brandon Davies

    Article

    Chi et al Mol Metab. 2017 Oct;6(10):1137-1149. doi

    Insert

    ANGPTL8 (ANGPTL8 Human)
    

    Use

    Lentiviral

    Tags

    6x His and V5

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    May 21, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pUAST cyto Grx1-roGFP2

    Plasmid

    #64994

    Purpose

    for expression of cytosolic Grx1-roGFP2 with the Gal4 expression system in drosophila

    Depositor

    Tobias Dick

    Article

    Albrecht et al Cell Metab. 2011 Dec 7;14(6):819-29. doi

    Insert

    Glutaredoxin-1 (GLRX Human)
    

    Use

    Tags

    roGFP2

    Expression

    Insect

    Mutation

    Promoter

    Available Since

    July 8, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pGEN25-Ub Hs

    Plasmid

    #83232

    Purpose

    pHis-par2-Ub Hs

    Depositor

    Gali Prag

    Article

    Levin-Kravets et al Nat Methods. 2016 Nov;13(11):945-952. do

    Insert

    Ubiqutin (UBC Human)
    

    Use

    Tags

    His

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    Nov. 22, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. mRuby2-CENPB-N-22

    Plasmid

    #55893

    Purpose

    Localization: Nucleus/Centromeres, Excitation: 559, Emission: 600

    Depositor

    Michael Davidson

    Article

    Lam et al Nat Methods. 2012 Sep 9. doi: 10.1038/nm

    Insert

    CENPB (CENPB Human)
    

    Use

    Tags

    mRuby2

    Expression

    Mammalian

    Mutation

    aa 1-169 of CENPB (DNA Binding Domain)

    Promoter

    CMV

    Available Since

    April 1, 2015

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

14. mRuby2-Calnexin-N-14

    Plasmid

    #55891

    Purpose

    Localization: Endoplasmic Reticulum, Excitation: 559, Emission: 600

    Depositor

    Michael Davidson

    Article

    Lam et al Nat Methods. 2012 Sep 9. doi: 10.1038/nm

    Insert

    Calnexin (CANX Human)
    

    Use

    Tags

    mRuby2

    Expression

    Mammalian

    Mutation

    I474N in Calnexin

    Promoter

    CMV

    Available Since

    March 12, 2015

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

15. mOrange2-Golgi-7

    Plasmid

    #57959

    Purpose

    Localization: Gal-T - Golgi Complex, Excitation: 549, Emission: 565

    Depositor

    Michael Davidson

    Article

    Shaner et al Nat Methods. 2008 Jun;5(6):545-51. doi:

    Insert

    Golgi (B4GALT1 Human)
    

    Use

    Tags

    mOrange2

    Expression

    Mammalian

    Mutation

    aa 1-82 of NM_001497.3

    Promoter

    CMV

    Available Since

    Nov. 4, 2014

    Availability

    Academic Institutions and Nonprofits only

16. pcDNA-Flag1-3HABeta2-mEos2

    Plasmid

    #114187

    Purpose

    studying clustering of Beta2 receptors by superresolution microscopy (PALM)

    Depositor

    Aleksandra Radenovic

    Article

    Annibale et al Nat Methods. 2011 Jun 12;8(7):527-8. doi

    Insert

    Adrenergic receptor beta 2 (ADRB2 Human)
    

    Use

    Tags

    3HA, FLAG, and mEOS2

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    Oct. 3, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pAAV-VTKS2

    Plasmid

    #170853

    Purpose

    AAV backbone for Cre-dependent transgene expression of any transgene in neurons under the control of the hSyn1 promoter.

    Depositor

    Jordane Dimidschstein

    Article

    Pouchelon et al Cell Rep Methods. 2022 May 26;2(6):10022

    Type

    Empty backbone

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    May 26, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pAAV-Ef1a-DIO hChR2(E123A)-mCherry

    Plasmid

    #35508

    Purpose

    AAV expression of EF1a-driven, cre-dependent, hChR2 variant CheTA 2.0 for ultrafast optogenetic control.

    Depositor

    Karl Deisseroth

    Article

    Mattis et al Nat Methods. 2011 Dec 18;9(2):159-72. do

    Insert

    hChR2
    

    Use

    AAV

    Tags

    mCherry

    Expression

    Mammalian

    Mutation

    E123A

    Promoter

    Ef1a

    Available Since

    April 18, 2012

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. MERS-coV-NSP3-c001

    Plasmid

    #173085

    Purpose

    Expression of recombinant fusion protein in E. coli

    Depositor

    Lari Lehtiö

    Article

    Sowa et al Cell Rep Methods. 2021 Dec 20;1(8):10012

    Insert

    rep (orf1ab Middle East respiratory syndrome-related coronavirus (isolate United Kingdom/H123990006/2012) (MERS-CoV))
    

    Use

    Tags

    mCerulean

    Expression

    Bacterial

    Mutation

    D1109-D1275, codon optimized

    Promoter

    Available Since

    Dec. 9, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pAAV.CAG.Flex.iGluSnFr.WPRE.SV40

    Plasmid

    #98932

    Purpose

    Cre dependent AAV expression of glutamate sensor from GFAP promoter ** A newer version of this sensor is available. Please see iGluSnFR3 plasmids at https://www.addgene.org/browse/article/28220233/ **

    Depositor

    Loren Looger

    Article

    Marvin et al Nat Methods. 2013 Feb;10(2):162-70. doi:

    Has Service

    AAV1, AAV5, and AAV9

    Insert

    iGluSnFr
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available Since

    Jan. 31, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge