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phlFd-mLin

Plasmid

#140793

Purpose

CMV-phlFo2-phlFR-2A-mCherry mammalian synthetic gene circuit

Depositor

Gabor Balazsi

Article

Szenk et al ACS Synth Biol. 2020 Apr 17;9(4):930-939

Insert

phlFd-mLinearizer

Expression

Mammalian

Promoter

CMV

Available Since

May 4, 2020

Availability

Academic Institutions and Nonprofits only

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pBS-TRE-CLIPf-WPRE

Plasmid

#104107

Purpose

Expresses CLIPf under the TRE promoter in mammalian cells.

Depositor

Takeshi Imai

Article

Sakaguchi et al eLife. 2018 Nov 20;7. pii: 40350. doi: 1

Insert

CLIPf

Expression

Mammalian

Promoter

TRE

Available Since

June 22, 2018

Availability

Academic Institutions and Nonprofits only

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pCMV-MBP-yATOM_mCherry

Plasmid

#209707

Purpose

Mammalian expression of y-ATOM biosensor for mCherry

Depositor

Stewart Loh

Article

Sekhon et al Nat Methods. 2023 Nov 9. doi: 10.1038/s4

Insert

MBP-yATOM_mCherry

Tags

MBP

Expression

Mammalian

Available Since

Nov. 13, 2023

Availability

Academic Institutions and Nonprofits only

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pEntr-SRFflbio

Plasmid

#32971

Depositor

William Pu

Article

He et al Proc Natl Acad Sci U S A. 2011 Apr 5. 10

Insert

SRF (SRF Human)

Use

Gateway

Tags

flag-bio

Available Since

Jan. 9, 2012

Availability

Academic Institutions and Nonprofits only

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pCRII-MBII85 probe

Plasmid

#67646

Purpose

antisense RNA probe for RNase protection or nothern

Depositor

Stefan Stamm

Article

Falaleeva et al Gene. 2015 Jul 26. pii: S0378-1119(15)00

Insert

MBII85 snoRNA

Use

For in vitro transcription

Available Since

Aug. 4, 2015

Availability

Academic Institutions and Nonprofits only

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p3E-GFP no-pA

Plasmid

#80813

Purpose

GFP without pA

Depositor

Philip Washbourne

Article

Fowler et al PLoS One. 2016 Aug 8;11(8):e0159277. doi

Insert

GFP no pA

Expression

Mammalian

Promoter

GFP

Available Since

Nov. 1, 2016

Availability

Academic Institutions and Nonprofits only

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pAG416-GAL-TDP-43

Plasmid

#181709

Purpose

Galactose inducible expression of TDP-43

Depositor

Alejandro Chavez

Insert

TDP-43 (TARDBP Human)

Expression

Yeast

Available Since

April 24, 2024

Availability

Academic Institutions and Nonprofits only

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pUS

Plasmid

#153416

Purpose

Backbone for cloning downstream of UCOE-SFFV

Depositor

Maki Nakayama

Article

Landry et al Bio Protoc. 2021 Jan 20;11(2):e3883. doi

Type

Empty backbone

Use

Lentiviral

Expression

Mammalian

Available Since

July 16, 2020

Availability

Academic Institutions and Nonprofits only

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LI D-E mOrange + link c2

Plasmid

#54421

Depositor

Martin Parniske

Article

Binder et al PLoS One. 2014 Feb 13;9(2):e88218. doi:

Insert

16 aa GS linker + mOrange

Use

Cloning vector

Available Since

June 4, 2014

Availability

Academic Institutions and Nonprofits only

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p3E-CMVmin:nlsGFP pA

Plasmid

#80821

Purpose

CMVmin-promoted nlsGFP with pA

Depositor

Philip Washbourne

Article

Fowler et al PLoS One. 2016 Aug 8;11(8):e0159277. doi

Insert

p3E-CMV-nlsGFP pA

Expression

Mammalian

Promoter

CMVmin

Available Since

Nov. 1, 2016

Availability

Academic Institutions and Nonprofits only

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p70rg

Plasmid

#17827

Depositor

Jay Keasling

Article

Pfleger et al Nat Biotechnol. 2006 Aug . 24(8):1027-32

Insert

E. coli optimized red flourescent protein (RFPec), GFPuv

Use

Synthetic Biology

Expression

Bacterial

Available Since

May 14, 2008

Availability

Academic Institutions and Nonprofits only
pET28a-TmCA-P(11)4

Plasmid

#185783

Purpose

Encodes thermostable carbonic anhydrase enzyme linked to P(11)4 self-assembling peptide via a GS-linker sequence

Depositor

Lizhong He

Article

Shanbhag et al Adv. Funct. Mater. 2022, 32, 2108039

Insert

TmCA-P(11)4

Tags

His-tag

Expression

Bacterial

Promoter

T7

Available Since

July 22, 2022

Availability

Academic Institutions and Nonprofits only

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pCMV-MBP-yATOM_Ras

Plasmid

#209709

Purpose

Mammalian expression of y-ATOM biosensor for HRas

Depositor

Stewart Loh

Article

Sekhon et al Nat Methods. 2023 Nov 9. doi: 10.1038/s4

Insert

MBP-yATOM_Ras

Tags

MBP

Expression

Mammalian

Available Since

Nov. 2, 2023

Availability

Academic Institutions and Nonprofits only

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BSP608

Plasmid

#122253

Purpose

expression vector for mNeptune under EFT3 promoter

Depositor

Alexander Mendenhall

Article

Sands et al Transl Med Aging. 2018 Jan;2:1-10. doi:

Insert

mNeptune

Expression

Worm

Promoter

EFT3

Available Since

April 8, 2019

Availability

Academic Institutions and Nonprofits only

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pUHD-SB-mAID/Zeo

Plasmid

#171681

Purpose

TRE-controlled mAID-fusion sleeping beauty transposon vector with selection marker (zeocin)

Depositor

Randy Poon

Article

Yeung et al Mol Biol Cell. 2021 May 12:mbcE21020051.

Type

Empty backbone

Tags

mAID

Expression

Mammalian

Promoter

TRE

Available Since

July 26, 2021

Availability

Academic Institutions and Nonprofits only

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LI D-E HA

Plasmid

#54449

Depositor

Martin Parniske

Article

Binder et al PLoS One. 2014 Feb 13;9(2):e88218. doi:

Insert

human influenza hemagglutinin (HA) tag (YPYDVPDYA)

Use

Cloning vector

Available Since

June 18, 2014

Availability

Academic Institutions and Nonprofits only

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LI B-C HA

Plasmid

#54448

Depositor

Martin Parniske

Article

Binder et al PLoS One. 2014 Feb 13;9(2):e88218. doi:

Insert

human influenza hemagglutinin (HA) tag (YPYDVPDYA)

Use

Cloning vector

Available Since

June 18, 2014

Availability

Academic Institutions and Nonprofits only

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pMMRSF17

Plasmid

#20178

Depositor

Hideo Iwai

Article

Muona et al Chembiochem. 2008 Dec 15. 9(18):2958-61.

Insert

C-CrkII nSH3 (CRK Human)

Tags

NpuNdelC16 and Smt3

Expression

Bacterial

Available Since

Aug. 17, 2009

Availability

Academic Institutions and Nonprofits only

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pQLinkHD_EBFP2

Plasmid

#118853

Purpose

fluorescent protein expression with an N-terminal His-tag

Depositor

Marc Birtwistle

Article

Holzapfel et al ACS Comb Sci. 2018 Nov 12;20(11):653-659

Insert

EBFP2

Tags

His-tag

Expression

Bacterial

Mutation

EBFP1.2-I128V/V150I/ D155V/V224R

Available Since

Dec. 12, 2018

Availability

Academic Institutions and Nonprofits only

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pYCTK019

Plasmid

#176723

Purpose

The plasmid is part of a GG toolkit for cell-cell communication in S. cerevisiae. This plasmid is an L0 part plasmid and contains the Ste2 gene of Candida albicans (receiver part).

Depositor

Victor Sourjik

Article

Krink et al bioRxiv 2021

Insert

ste2Ca

Use

Synthetic Biology

Mutation

Codon-optimized for expression in S. cerevisiae

Available Since

Jan. 6, 2022

Availability

Academic Institutions and Nonprofits only

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