We narrowed to 51,138 results for: ANG

1. pLenti-CaMKIIa-hChR2(H134R)-EYFP-WPRE

   Plasmid

   #20944

   Purpose

   3rd gen lentiviral expression of humanized ChR2 with H134R mutation fused to EYFP driven by CaMKIIa promoter for optogenetic activation

   Depositor

   Karl Deisseroth

   Article

   Zhang et al Nature. 2007 Apr 5. 446(7136):633-9.

   Insert

   channelrhodopsin-2
   

   Use

   Lentiviral

   Tags

   EYFP

   Expression

   Mammalian

   Mutation

   H134R

   Available Since

   May 13, 2009

   Availability

   Academic Institutions and Nonprofits only

2. NAX_CAGGS_CAS9_m4_KRAB-MeCP2_mcherry

   Plasmid

   #167902

   Purpose

   PiggyBac compatible plasmid expressing dCas9-KRAB-MeCP2 expression plasmid

   Depositor

   Alejandro Chavez, Nan Cher Yeo

   Article

   Nghi Dang et al Synthetic Biology, Volume 6, Issue 1, 20

   Insert

   dCas9-KRAB-MeCP2
   

   Use

   CRISPR

   Mutation

   D10A, D839A, H840A, N863A

   Available Since

   Sept. 12, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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3. pCMV-BE-VQRCas9

   Plasmid

   #101740

   Purpose

   expression of an optimized Cas9 for base-editing in zebrafish

   Depositor

   Shuo Lin

   Article

   Zhang et al Nat Commun. 2017 Jul 25;8(1):118. doi: 1

   Insert

   rAPOBEC1-XTEN-zCas9(VQR)-UGI-NLS
   

   Use

   CRISPR

   Expression

   Mammalian

   Mutation

   D10A, VQR mutations

   Promoter

   CMV

   Available Since

   Jan. 24, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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4. U6-5' MS2-CircRNA

   Plasmid

   #213750

   Purpose

   For circular RNA-mediated prime editor using U6-5' MS2-CircRNA in HEK293T cells

   Depositor

   Caixia Gao

   Article

   Liang et al Nat Biotechnol. 2024 Jan 10. doi: 10.103

   Insert

   5' ribozyme, 5' ligation sequences, 5' MS2, 3' ligation sequences, 3' ribozyme
   

   Use

   CRISPR

   Expression

   Mammalian

   Promoter

   U6

   Available Since

   Feb. 7, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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5. pMXs-KDM4D

   Plasmid

   #188234

   Purpose

   Express human KDM4D in mammalian cells

   Depositor

   Young Tang

   Article

   Su et al Int J Mol Sci. 2021 Sep 28;22(19). pii:

   Insert

   Lysine demethylase 4D (KDM4D Human)
   

   Use

   Retroviral

   Expression

   Mammalian

   Available Since

   Oct. 20, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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6. pREDIT_Cas9n-MS2-BB_BbsI

   Plasmid

   #164804

   Purpose

   REDIT backbone for nickase Cas9n, pU6-MS2-gRNA-backbone(BbsI)-CBH-SpCas9n(D10A)-T2A-EGFP

   Depositor

   Le Cong

   Article

   Wang et al Nucleic Acids Res. 2021 Apr 6;49(6):e36.

   Type

   Empty backbone

   Expression

   Mammalian

   Available Since

   Feb. 22, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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7. pEGFP-N1 gp78fl

   Plasmid

   #37375

   Depositor

   Allan Weissman

   Article

   Fang et al Proc Natl Acad Sci U S A. 2001 Dec 4. 98

   Insert

   gp78 (AMFR Human)
   

   Tags

   EYFP

   Expression

   Mammalian

   Mutation

   P179L and F262L

   Available Since

   Nov. 27, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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8. pM122: pAAV-EFS-CasRx

   Plasmid

   #166870

   Purpose

   AAV vector for expressing CasRx

   Depositor

   Guei-Sheung Liu

   Article

   Chuang et al Front Cell Dev Biol. 2021 Jun 11;9:66787

   Insert

   RfxCas13d
   

   Use

   AAV and CRISPR

   Tags

   HA and NLS

   Expression

   Mammalian

   Promoter

   EFS

   Available Since

   July 1, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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9. E407V Nuclear Myosin I YFP

   Plasmid

   #60242

   Purpose

   Expresses a E407V mutant of the nuclear isoform of myosin IC attached to YFP and an NLS, which has impaired actin binding and motor activities

   Depositor

   Primal de Lanerolle

   Article

   Chuang et al Curr Biol. 2006 Apr 18;16(8):825-31.

   Insert

   Nuclear Myosin 1 (Myo1c Mouse)
   

   Tags

   EYFP and NLS

   Expression

   Mammalian

   Mutation

   E407V

   Available Since

   Nov. 21, 2014

   Availability

   Academic Institutions and Nonprofits only

10. pAAV-EF1a-Flp-DOG-NW

    Plasmid

    #75469

    Purpose

    Flp recombinase dependent on GFP (Flp-DOG) for rAAV production and expression in mammalian cells

    Depositor

    Connie Cepko

    Article

    Tang et al Elife. 2016 May 20;5. pii: e15312. doi:

    Has Service

    AAV1

    Insert

    Flp-DOG
    

    Use

    AAV and Synthetic Biology

    Expression

    Mammalian

    Promoter

    EF-1a

    Available Since

    April 25, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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11. pcDNA3-Akt-Scramble

    Plasmid

    #175014

    Purpose

    Negative-control construct for Akt-STOPS.

    Depositor

    Jin Zhang

    Article

    Zhou et al Nat Commun. 2020 Nov 30;11(1):6088. doi:

    Insert

    mCherry-Akt-Scramble
    

    Tags

    6xHIS - T7 tag (gene 10 leader) - Xpress (TM) tag…

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Jan. 7, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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12. pUAST td tomato Rab5 WT

    Plasmid

    #58446

    Purpose

    expression of drosophila td tomato Rab5

    Depositor

    Matthew Scott

    Article

    Zhang et al Genetics. 2007 Jun;176(2):1307-22. Epub

    Insert

    Rab5 (Rab5 Fly)
    

    Tags

    td tomato

    Expression

    Insect

    Promoter

    GAL4

    Available Since

    July 21, 2014

    Availability

    Academic Institutions and Nonprofits only

13. pEN-L1-PJ23119-PplpT-sfGFP-TrrfB-BsaI-Scaf-L2

    Plasmid

    #196250

    Purpose

    Plasmid for cloning of a CRISPR-Cas9 guide RNA gene under promoter PJ23119

    Depositor

    Bing Yang

    Article

    Li et al Commun Biol. 2023 Jan 17;6(1):56. doi: 1

    Insert

    attL1-GlpT-scGFP-attL2
    

    Expression

    Bacterial

    Promoter

    J23119 promoter

    Available Since

    May 12, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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14. AAV-phsyn-FLEX-KASH-EGFP-U6-shRNA

    Plasmid

    #129708

    Purpose

    Express cre-dependent KASH-EGFP and scramble shRNA

    Depositor

    Yi Zhang

    Article

    Tuesta et al Nat Commun. 2019 Jun 7;10(1):2508. doi:

    Insert

    KASH-EGFP
    

    Use

    AAV

    Tags

    EGFP

    Expression

    Mammalian

    Promoter

    U6

    Available Since

    Aug. 21, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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15. NAX_CAGGS_CAS9_m4_VPR_KanR neo

    Plasmid

    #167885

    Purpose

    PiggyBac compatible plasmid expressing dCas9-VPR

    Depositor

    Alejandro Chavez, Nan Cher Yeo

    Article

    Nghi Dang et al Synthetic Biology, Volume 6, Issue 1, 20

    Insert

    dCas9-VPR
    

    Use

    CRISPR

    Mutation

    D10A, D839A, H840A, N863A

    Available Since

    Feb. 9, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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16. pmiR-report-hVEGFA

    Plasmid

    #112553

    Purpose

    3'UTR reporter assay

    Depositor

    Shusheng Wang

    Article

    Zhou et al Mol Ther. 2016 Oct;24(10):1823-1835. doi

    Insert

    VEGFA (VEGFA Human)
    

    Use

    Luciferase

    Available Since

    July 6, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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17. S1-Fc

    Plasmid

    #164220

    Purpose

    Expresses soluble, dimeric Fc-His fusion protein for the original SARS-CoV2 Spike S1 region, along with dTomato reporter

    Depositor

    Sriram Neelamegham

    Article

    Yang et al Elife. 2020 Oct 26;9. pii: 61552. doi: 1

    Insert

    Spike S1 domain (S SARS-CoV)
    

    Use

    Lentiviral

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Feb. 1, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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18. pcDNA3-Nuc-ExRai-AMPKAR

    Plasmid

    #192450

    Purpose

    ExRai-AMPKAR targeted to the nucleus.

    Depositor

    Jin Zhang

    Article

    Schmitt et al Nat Commun. 2022 Jul 5;13(1):3856. doi:

    Insert

    ExRai-AMPKAR-NLS
    

    Tags

    6xHIS - T7 tag (gene 10 leader) - Xpress (TM) tag…

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Dec. 7, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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19. AAV-phsyn-FLEX-KASH-EGFP-U6-ShGmeb1

    Plasmid

    #129706

    Purpose

    Express cre-dependent KASH-EGFP and Gmeb1 shRNA

    Depositor

    Yi Zhang

    Article

    Tuesta et al Nat Commun. 2019 Jun 7;10(1):2508. doi:

    Insert

    KASH-EGFP
    

    Use

    AAV

    Tags

    EGFP

    Expression

    Mammalian

    Promoter

    U6

    Available Since

    Aug. 21, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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20. NAX_CAGGS_CAS9_m4_KRAB-MeCP2_EYFP

    Plasmid

    #167898

    Purpose

    PiggyBac compatible plasmid expressing dCas9-KRAB-MeCP2 expression plasmid

    Depositor

    Alejandro Chavez, Nan Cher Yeo

    Article

    Nghi Dang et al Synthetic Biology, Volume 6, Issue 1, 20

    Insert

    dCas9-KRAB-MeCP2
    

    Use

    CRISPR

    Mutation

    D10A, D839A, H840A, N863A

    Available Since

    Sept. 14, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pLAC

    Plasmid

    #45072

    Purpose

    lacritin-intein fusion plasmid coding for intact mature lacritin without signal peptide

    Depositor

    Gordon Laurie

    Article

    Wang et al J Cell Biol. 2006 Aug 28;174(5):689-700.

    Insert

    Lacritin (LACRT Human)
    

    Use

    Protein expression vector

    Tags

    Intein

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Sept. 25, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pRetroSuper-shSrc

    Plasmid

    #26982

    Depositor

    Joan Massague

    Article

    Zhang et al Cancer Cell. 2009 Jul 7. 16(1):67-78.

    Insert

    shRNA Src (SRC Human)
    

    Use

    RNAi and Retroviral

    Expression

    Mammalian

    Available Since

    Dec. 17, 2010

    Availability

    Academic Institutions and Nonprofits only

23. pQE-30-RexYFP

    Plasmid

    #141070

    Purpose

    Bacterial expression of genetically encoded fluorescent indicator for measuring NAD+/NADH ratio

    Depositor

    Vsevolod Belousov

    Article

    Bilan et al Biochim Biophys Acta. 2013 Nov 25. pii:

    Insert

    RexYFP
    

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    changed Leucine 169 to Proline, changed Tyrosine …

    Promoter

    T5

    Available Since

    March 1, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pcDNA3-AktAR2-NES

    Plasmid

    #175011

    Purpose

    Cytosol-targeted AktAR2.

    Depositor

    Jin Zhang

    Article

    Zhou et al Nat Commun. 2020 Nov 30;11(1):6088. doi:

    Insert

    AktAR-NES
    

    Tags

    6xHIS - T7 tag (gene 10 leader) - Xpress (TM) tag…

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Oct. 7, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pcDNA3-H2A-Akt-STOPS

    Plasmid

    #175010

    Purpose

    Akt-STOPS targeted to the nucleus via histone H2A.

    Depositor

    Jin Zhang

    Article

    Zhou et al Nat Commun. 2020 Nov 30;11(1):6088. doi:

    Insert

    H2A-mCherry-Akt-STOPS
    

    Tags

    Histone H2A and mCherry

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Jan. 7, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. LRP6-m5-pCS2-VSVG

    Plasmid

    #27283

    Depositor

    Xi He

    Article

    Tamai et al Mol Cell. 2004 Jan 16. 13(1):149-56.

    Insert

    low density lipoprotein receptor-related protein 6 (LRP6 Human)
    

    Use

    Xenopus injection

    Tags

    VSVG

    Mutation

    Changed S/T of all five PPP(S/T)P motifs to Alani…

    Available Since

    March 3, 2011

    Availability

    Academic Institutions and Nonprofits only

27. pcDNA3-AktAR2-NLS

    Plasmid

    #175012

    Purpose

    Nuclear-targeted AktAR2.

    Depositor

    Jin Zhang

    Article

    Zhou et al Nat Commun. 2020 Nov 30;11(1):6088. doi:

    Insert

    AktAR-NLS
    

    Tags

    6xHIS - T7 tag (gene 10 leader) - Xpress (TM) tag…

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Sept. 23, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. U6-3' MS2-CircRNA

    Plasmid

    #213751

    Purpose

    For circular RNA-mediated prime editor using U6-3' MS2-CircRNA in HEK293T cells

    Depositor

    Caixia Gao

    Article

    Liang et al Nat Biotechnol. 2024 Jan 10. doi: 10.103

    Insert

    5' ribozyme, 5' ligation sequences, 3' MS2, 3' ligation sequences, 3' ribozyme
    

    Use

    CRISPR

    Expression

    Mammalian

    Promoter

    U6

    Available Since

    Feb. 7, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. NAX_CAGGS_CAS9_m4_VPR_mcherry

    Plasmid

    #167886

    Purpose

    PiggyBac compatible plasmid expressing dCas9-VPR

    Depositor

    Alejandro Chavez, Nan Cher Yeo

    Article

    Nghi Dang et al Synthetic Biology, Volume 6, Issue 1, 20

    Insert

    dCas9-VPR
    

    Use

    CRISPR

    Mutation

    D10A, D839A, H840A, N863A

    Available Since

    Sept. 13, 2022

    Availability

    Academic Institutions and Nonprofits only

30. pRetroSuper-shFyn

    Plasmid

    #26985

    Depositor

    Joan Massague

    Article

    Zhang et al Cancer Cell. 2009 Jul 7. 16(1):67-78.

    Insert

    shRNA Fyn (FYN Human)
    

    Use

    RNAi and Retroviral

    Expression

    Mammalian

    Available Since

    Dec. 17, 2010

    Availability

    Academic Institutions and Nonprofits only