We narrowed to 13,186 results for: BASE;

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AIO-GFP

Plasmid

#74119

Purpose

All-in-One plasmid encoding dual U6 promoter-driven sgRNAs and EGFP-coupled Cas9-D10A nickase to enhance efficient and accurate genome editing

Depositor

Steve Jackson

Article

Chiang et al Sci Rep. 2016 Apr 15;6:24356. doi: 10.10

Type

Empty backbone

Use

CRISPR

Expression

Mammalian

Available Since

May 5, 2016

Availability

Academic Institutions and Nonprofits only

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mNeonGreen-3xrGBD

Plasmid

#176091

Purpose

Visualization of active Rho

Depositor

Dorus Gadella

Article

Mahlandt et al J Cell Sci. 2021 Aug 6. pii: 271216. doi

Insert

rhotekin (Rtkn Mouse)

Expression

Mammalian

Promoter

CMVdel

Available Since

Oct. 25, 2021

Availability

Academic Institutions and Nonprofits only

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pU6-GG-gRNA

Plasmid

#226860

Purpose

Human gRNA expression vector backbone with BsmBI Golden Gate site

Depositor

Alexis Komor

Article

Osgood et al Methods Enzymol. 2025;712:351-404. doi:

Insert

Sp-gRNA

Expression

Mammalian

Promoter

hU6

Available Since

Feb. 10, 2025

Availability

Academic Institutions and Nonprofits only

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P307-FBP_6

Plasmid

#139697

Purpose

T-DNA encoding Fungal Bioluminescent Pathway Components, control pathway

Depositor

Daniel Voytas

Article

Khakhar et al Elife. 2020 Mar 25;9. pii: 52786. doi: 1

Insert

pLHB1B1:NnCPH:tSlH4, p35S_Short_TMV 5':AsNpga:tAtug7, pAtUbi1:NnH3H:tAtuNos, pCmYLCV:NnHisps:tHsp, p35S:NnLuz:tAtuOcs

Use

Luciferase

Expression

Plant

Promoter

various

Available Since

May 29, 2020

Availability

Academic Institutions and Nonprofits only

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pLV hUbC-VP64 dCas9 VP64-T2A-GFP

Plasmid

#59791

Purpose

Co-expresses human optimized S. pyogenes dCas9 fused to two copies of VP64 and GFP

Depositor

Charles Gersbach

Article

Kabadi et al Nucleic Acids Res. 2014 Aug 13. pii: gku

Insert

humanized VP64 dead Cas9 VP64 T2A GFP

Use

CRISPR and Lentiviral

Tags

Flag

Expression

Mammalian

Mutation

D10A and H840A

Available Since

Oct. 23, 2014

Availability

Academic Institutions and Nonprofits only

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pSW002-Pc-mTurquoise2

Plasmid

#108235

Purpose

Broad host bacterial expression vector with constitutive Pc promoter. Expresses mTurquoise2; codon optimized for P. fluorescens.

Depositor

Rosemarie Wilton

Article

Wilton et al Front Plant Sci. 2018 Feb 1;8:2242. doi:

Insert

Pc promoter - mTurquoise2

Expression

Bacterial

Mutation

codon optimized for experession in P. fluorescens

Available Since

May 29, 2018

Availability

Academic Institutions and Nonprofits only

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pEF-BFP

Plasmid

#138272

Purpose

Expression of engineered BFP variant that undergoes conversion to GFP after targeted modification with a cytidine deaminase-based DNA base editor

Depositor

Xiao Wang

Article

Standage-Beier et al Nucleic Acids Res. 2019 Aug 20. pii: 555

Insert

BFP H66 PAM

Use

CRISPR

Expression

Mammalian

Mutation

H66, PAM motif

Promoter

Ef1a

Available Since

March 3, 2020

Availability

Academic Institutions and Nonprofits only

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pGM1190

Plasmid

#69994

Purpose

OriT containing mobilizable vector for thiostrepton induced gene expression in Streptomyces

Depositor

Günther Muth

Article

Muth et al Appl Microbiol Biotechnol. 2018 Nov;102(

Inserts

aac
tsr
rep
sso
TipA promoter
dso

Expression

Bacterial

Available Since

April 19, 2016

Availability

Academic Institutions and Nonprofits only

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pLVX-EF1a-EGFP-Emerin-IRES-Hygromycin

Plasmid

#134864

Purpose

Expresses EGFP-tagged nuclear envelope marker

Depositor

David Andrews

Article

Schormann et al J Cell Biol. 2020 Mar 2;219(3). pii: 133

Insert

emerin (EMD Human)

Use

Lentiviral

Tags

EGFP

Expression

Mammalian

Promoter

EF1a

Available Since

Jan. 26, 2021

Availability

Academic Institutions and Nonprofits only

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qTAG-AAVS1-PGK-Zeo

Plasmid

#227271

Purpose

Empty AAVS1 targeting donor for the insertion of Zeo and a medium strength PGK promoter. A CDS can be cloned adjacent to the promoter via restriction or gibson cloning using the MluI cut site.

Depositor

Laurence Pelletier

Article

Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

Type

Empty backbone

Use

CRISPR; Donor cassette

Expression

Mammalian

Available Since

Nov. 12, 2024

Availability

Academic Institutions and Nonprofits only

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pcDNA GFP10-ZIP-GFP11

Plasmid

#182243

Purpose

Tripartite split-GFP mammalian. Express leucine zipper with GFP10 and GFP11 (self-assembing with GFP1-9)

Depositor

Stéphanie Cabantous

Article

Cabantous et al Sci Rep. 2013 Oct 4;3:2854. doi: 10.1038

Insert

GFP10-ZIP-11

Expression

Mammalian

Promoter

CMV

Available Since

April 26, 2022

Availability

Academic Institutions and Nonprofits only

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mVenus-P115-C1

Plasmid

#112931

Purpose

p115RhoGEF tagged with mVenus for detection of Ga13 activity

Depositor

Dorus Gadella

Article

Mastop et al PLoS One. 2018 Mar 5;13(3):e0193705. doi

Insert

p115 RhoGEF

Tags

mVenus

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 9, 2018

Availability

Academic Institutions and Nonprofits only

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pUASTLOTattB_vhhGFP4::Vkg::mCherry

Plasmid

#163929

Purpose

Expression of GrabFP-ECM under control of UAS or LOP enhancer

Depositor

Markus Affolter

Article

Harmansa et al Elife. 2017 Apr 11;6. doi: 10.7554/eLife

Insert

Fusion of vhhGPF4 nanobody with Viking (Collagen IV) protein and mCherry fluorophor

Use

Cre/Lox

Tags

mCherry

Expression

Insect

Mutation

G1767D

Available Since

Feb. 9, 2021

Availability

Academic Institutions and Nonprofits only

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MT3

Plasmid

#105710

Purpose

Bacterial expression plasmids for production of recombinant metallothionein 3

Depositor

Artur Krężel

Article

Peris-Diaz et al J Proteome Res. 2021 Jan 1;20(1):776-785

Insert

Metallothionein 3 (MT3 Human)

Tags

Chiting Binding Domanin-Intein

Expression

Bacterial

Available Since

April 27, 2020

Availability

Academic Institutions and Nonprofits only

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mAzurite-16xRhoBAST

Plasmid

#196347

Purpose

Expression of mAzurite mRNA with 16xRhoBAST in 3'UTR

Depositor

Murat Sunbul

Article

Englert et al Nat Commun. 2023 Jun 30;14(1):3879. doi:

Insert

mAzurite-16xRhoBAST

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 15, 2023

Availability

Academic Institutions and Nonprofits only

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FB312

Plasmid

#203569

Purpose

TU for the expression of Nanoluciferase (Nluc) under the PgpdA promoter.

Depositor

Jose Marcos

Article

Gandia et al Microb Biotechnol. 2022 Feb;15(2):630-64

Insert

PgpdA:Nluc:Ttub

Use

Synthetic Biology

Available Since

Aug. 22, 2023

Availability

Academic Institutions and Nonprofits only

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pBT29-splitD

Plasmid

#122599

Purpose

clone M13 phage by SapI Golden Gate

Depositor

David Liu

Article

Thuronyi et al Nat Biotechnol. 2019 Jul 22. pii: 10.103

Insert

M13 bacteriophage genes

Expression

Bacterial

Available Since

July 29, 2019

Availability

Academic Institutions and Nonprofits only

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pBT29-splitC

Plasmid

#122598

Purpose

clone M13 phage by SapI Golden Gate

Depositor

David Liu

Article

Thuronyi et al Nat Biotechnol. 2019 Jul 22. pii: 10.103

Insert

M13 bacteriophage genes

Expression

Bacterial

Available Since

Aug. 21, 2019

Availability

Academic Institutions and Nonprofits only

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Red cGull

Plasmid

#191853

Purpose

Expresses biosensor for cGMP in mammalian cells

Depositor

Takashi Tsuboi

Article

Takizawa et al Commun Biol. 2022 Sep 5;5(1):833. doi: 1

Insert

Red cGull

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 17, 2023

Availability

Academic Institutions and Nonprofits only

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pCEP4-BaLgp160

Plasmid

#100919

Purpose

Mammalian expression plasmid for Env from the BaL HIV-1 isolate

Depositor

Erik Procko

Article

Heredia et al J Virol. 2019 Mar 20. pii: JVI.00219-19.

Insert

HIV-1 (BaL) Env

Tags

CD5 leader sequence

Expression

Mammalian

Mutation

Codon-optimized synthetic gene

Promoter

CMV

Available Since

March 25, 2019

Availability

Academic Institutions and Nonprofits only

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