We narrowed to 6,701 results for: proc
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Plasmid#114112Purposefusion protein of NanoLuc, Volvox channelrhodopsin-1, and enhanced yellow fluorescent protein for bioluminescent optogeneticsDepositorInsertNanoLuc; Volvox channelrhodopsin 1; EYFP
ExpressionMammalianPromoterCAGAvailable SinceAug. 5, 2019AvailabilityAcademic Institutions and Nonprofits only -
mVenus-proα1(I)
Plasmid#119845PurposeExpresses mouse Type I procollagen α1 chain (Col1a1) with mVenus replacing exon 2-3 retaining N-propeptide minor triple helix and N-terminal cleavage siteDepositorInsertType I procollagen α1 chain (Col1a1 Mouse)
TagsmVenusExpressionMammalianMutationCol1a1 exons 2-3 replaced by fluorescent tagPromoterCMVAvailable SinceJan. 28, 2019AvailabilityAcademic Institutions and Nonprofits only -
eGFP-proα2(I)-G610C
Plasmid#119827PurposeExpresses mouse Type I procollagen α2 chain (Col1a2) with Gly610Cys mutation and GFP between signal sequence and exon 6DepositorInsertType I procollagen α2 chain (Col1a2 Mouse)
TagseGFPExpressionMammalianMutationCol1a2 exons 2-5 replaced by fluorescent tag, Gly…PromoterCMVAvailable SinceJan. 28, 2019AvailabilityAcademic Institutions and Nonprofits only -
pQ-NFY-A-GB-HC
Plasmid#171081PurposeCo-expresses of NFY complex in bacteria. The resulting plasmid was used to generate a single expression construct encoding the three subunit NF-Y, with untagged NFYA, GST-NFYB, and 6-His-NFYCDepositorTagsGST and HisExpressionBacterialPromoterT5 promoterAvailable SinceJuly 19, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCR-HA-IKKbeta
Plasmid#15470DepositorAvailable SinceOct. 10, 2007AvailabilityAcademic Institutions and Nonprofits only -
pET28_6xHis-PARP1-CATdeltaHD
Plasmid#174811PurposeBacterial expression of a hyperactive PARP1 CAT lacking the autoinhibitory HD subdomainDepositorInsertPARP1-CATdeltaHD (PARP1 Human)
Tags6xHisExpressionBacterialMutationResidues 678-787 replaced by eight-residue linker…Available SinceSept. 23, 2021AvailabilityAcademic Institutions and Nonprofits only -
pLKO-puro-tetON-shOGDH 2497
Plasmid#110942PurposeTet-inducible shRNA targeting OGDHDepositorAvailable SinceOct. 31, 2018AvailabilityAcademic Institutions and Nonprofits only -
pLKO-puro-tetON-shOGDH 1714
Plasmid#110941PurposeTet-inducible shRNA targeting OGDHDepositorAvailable SinceNov. 2, 2018AvailabilityAcademic Institutions and Nonprofits only -
pLKO-puro-tetON-shOGDH 2956
Plasmid#110943PurposeTet-inducible shRNA targeting OGDHDepositorAvailable SinceOct. 31, 2018AvailabilityAcademic Institutions and Nonprofits only -
pRSF-DUET1-DHDDS/Sumo-NgBR
Plasmid#203162PurposeExpression of human cis-prenyltransferase complex for purificationDepositorTagsHis6-SUMOExpressionBacterialMutationtruncationAvailable SinceAug. 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
SOD1-VC
Plasmid#89758Purposeexpresses human SOD1 fused to the C-terminal part of VenusDepositorAvailable SinceMarch 31, 2020AvailabilityAcademic Institutions and Nonprofits only -
pFRLuc-CoV2-delStem1
Plasmid#177616PurposeDual luciferase reporter for SARS-CoV-2 programmed -1 ribosomal frameshifting. 3-nucleotide deletion in Stem 1 (negative control).DepositorInsertSARS-CoV-2 FSE
UseLuciferaseExpressionMammalianMutation3-nucleotide (UAC) deletion in Stem 1Available SinceDec. 10, 2021AvailabilityAcademic Institutions and Nonprofits only -
AAV-short_BrokenHeart (no BC)
Plasmid#86950PurposeAAV transfer plasmid containing the BrokenHeart construct: a hyperpiggybac donor transposon interrupting the tdTomato fluorophore. Transposase activity rescues coding sequence.DepositorInsertBrokenheart construct
UseAAVExpressionMammalianAvailable SinceApril 13, 2020AvailabilityAcademic Institutions and Nonprofits only -
pLL7.0: hITSN1(1159-1509)-tgRFPt-SSPB WT
Plasmid#60419PurposeMammalian Expression of ITSN DH/PH-tgRFPt-NanoDepositorInserthITSN1-tgRFPt-SSPB (ITSN1 Synthetic, Human)
UseCre/Lox and LentiviralTagstgRFPtExpressionMammalianMutationhITSN 1159-1509 // SSPB Y11K and A15EPromoterCMVAvailable SinceFeb. 27, 2015AvailabilityAcademic Institutions and Nonprofits only -
pFRLuc-CoV2-FLucStop
Plasmid#177617PurposeDual luciferase reporter for SARS-CoV-2 programmed -1 ribosomal frameshifting. In-frame stop codon inserted after FLuc (negative control).DepositorInsertSARS-CoV-2 FSE
UseLuciferaseExpressionMammalianMutationIn-frame stop codon added between FLuc and FSEAvailable SinceDec. 10, 2021AvailabilityAcademic Institutions and Nonprofits only -
pINDUCER12 (miR-RUL)
Plasmid#46935PurposeInducible lentiviral gene silencing vector. Insert PheSGly294, (XhoI to EcoRI;1.4kb),can be replaced w mir30 based hairpin of interestDepositorInsertPheS Gly294
UseLentiviralAvailable SinceNov. 4, 2014AvailabilityAcademic Institutions and Nonprofits only -
pCAGGS-PHFF
Plasmid#100280PurposeExpression vector for phycocyanobilin (PCB) synthesis in mammalian cells.DepositorInsertMTS-PcyA-FLAG-P2A-MTS-HA-HO1-P2A-MTS-Myc-Fd-P2A-MTS-Fnr-T7 (synthetic genes)
TagsFLAG, T7 and Mitochondria-targeting sequence (MTS…ExpressionMammalianMutationAll genes are codon-optimized for expression in h…PromoterCAG promoterAvailable SinceSept. 20, 2017AvailabilityAcademic Institutions and Nonprofits only -
mCerulean-proα1(I)
Plasmid#119842PurposeExpresses mouse Type I procollagen α1 chain (Col1a1) with mCerulean replacing exon 2-3 retaining N-propeptide minor triple helix and N-terminal cleavage siteDepositorInsertType I procollagen α1 chain (Col1a1 Mouse)
TagsmCeruleanExpressionMammalianMutationCol1a1 exons 2-3 replaced by fluorescent tagPromoterCMVAvailable SinceJan. 28, 2019AvailabilityAcademic Institutions and Nonprofits only -
pAG106 Lyn11-FAST
Plasmid#130721PurposeExpresses FAST (also called YFAST) fused to Lyn11 sequence in mammalian cellsDepositorInsertlyn11-FAST
Tagsmyc-tagExpressionMammalianPromoterCMVAvailable SinceSept. 13, 2019AvailabilityAcademic Institutions and Nonprofits only -
CRY2-INPP5E
Plasmid#79562Purposeexpression of CRY2PHR fused to inositol 5-phosphatase domain of human INPP5E with mutated C-terminal CaaX-motifDepositorInsertINPP5E (INPP5E Human)
TagsCRY2 (photolyase homology region domain of Arabid…ExpressionMammalianMutationC-terminal region, aa 214_644, C641A mutation to …PromoterCMVAvailable SinceJuly 11, 2016AvailabilityAcademic Institutions and Nonprofits only