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GFP-RNF51

Plasmid

#126546

Purpose

Expresses GFP-tagged RNF51 in mammalian cells

Depositor

Michele Pagano

Article

Rona et al Elife. 2018 Jul 9;7. pii: 38771. doi: 10

Insert

RNF51 (BMI1 Human)

Use

Retroviral

Tags

GFP

Expression

Mammalian

Promoter

LTR

Available Since

July 3, 2019

Availability

Academic Institutions and Nonprofits only

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pRRLNeo-pEF1a-p53ash-mCerulean

Plasmid

#69579

Purpose

Expresses p53 tagged with mCerulean

Depositor

Galit Lahav

Article

Gaglia et al Proc Natl Acad Sci U S A. 2013 Sep 17;11

Insert

p53 (TP53 Human)

Use

Lentiviral

Tags

mCerulean

Expression

Mammalian

Mutation

7 silent mutations in p53 DNA to prevent targetin…

Promoter

EF1alpha

Available Since

Oct. 20, 2017

Availability

Academic Institutions and Nonprofits only

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TadA-NG-CBE

Plasmid

#227420

Purpose

C-to-T base editing in mammalian cells

Depositor

Yang Sun

Article

Liu et al Adv Sci (Weinh). 2024 Sep 19:e2405628. d

Insert

TadA-nSpCas9-NG-2xUGI (AICDA Human)

Use

CRISPR

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 25, 2024

Availability

Academic Institutions and Nonprofits only

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DRD1-DuET

Plasmid

#213227

Purpose

The DuET construct encodes the named human GPCR engineered with a cleaved hemagglutinin signal peptide, a 5' FLAG and a 3' 1D4 epitope tag optimized for expression in mammalian cells and proteomics studies.

Depositor

Thomas Sakmar

Article

Dahl et al Sci Adv. 2023 May 3;9(18):eadf9297. doi:

Insert

DRD1 (DRD1 Human)

Tags

1D4 and FLAG

Expression

Mammalian

Promoter

CMV, T7

Available Since

April 30, 2024

Availability

Academic Institutions and Nonprofits only

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tetO-Bcl2-IRES-tdtomato(TET005)

Plasmid

#117857

Purpose

This plasmid expresses Bcl2 under the activation of tTA trans-activator along with fluorescent marker tdTomato.

Depositor

C. Ron Yu

Article

Wu et al Neuron. 2018 Dec 5;100(5):1066-1082.e6.

Insert

Bcl2 (Bcl2 Mouse)

Use

Tta/teto

Expression

Mammalian

Promoter

tetO

Available Since

May 30, 2019

Availability

Academic Institutions and Nonprofits only

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mCherry-CAD

Plasmid

#73566

Purpose

Soluble red fluorescent activator of Orai1 channels

Depositor

Michael Cahalan

Article

Dynes et al Proc Natl Acad Sci U S A. 2016 Jan 12;11

Insert

mCherry-CAD (STIM1 342-448) (STIM1 Synthetic, Human)

Tags

6x His and mCherry

Expression

Mammalian

Promoter

CMV Immediate Early

Available Since

March 11, 2016

Availability

Academic Institutions and Nonprofits only

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pET-Sac-Aβ(M1-42/C18C33)

Plasmid

#173759

Purpose

Expresses the AβC18C33 peptide.

Depositor

James Nowick

Article

Zhang et al Biochemistry. 2022 Feb 15;61(4):252-264.

Insert

Aβ(M1-42/C18C33) (APP Human)

Expression

Bacterial

Mutation

changed valine 18 to cysteine; changed glycine 33…

Promoter

T7

Available Since

Aug. 26, 2021

Availability

Academic Institutions and Nonprofits only

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EF1a_HAND2_P2A_Hygro_Barcode

Plasmid

#120447

Purpose

Barcoded lentiviral vector to express HAND2 in mammalian cells under control of EF1a promoter. Barcoded for transcript detection in single cell RNA-seq.

Depositor

Prashant Mali

Article

Parekh et al Cell Syst. 2018 Nov 12. pii: S2405-4712(

Insert

HAND2 (HAND2 Human)

Use

Lentiviral

Promoter

EF1a

Available Since

Feb. 4, 2019

Availability

Academic Institutions and Nonprofits only

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pSpCas9(BB)-2A-Puro(PX459)-GJA1 sgRNA

Plasmid

#164471

Purpose

Expresses sgRNA targeting human GJA1 locus

Depositor

Markus Kuehn

Article

Cheng et al Stem Cells. 2022 Mar 9. pii: 6545973. do

Insert

Gap junction alpha-1 (GJA1) (GJA1 Human)

Expression

Mammalian

Promoter

U6

Available Since

May 16, 2022

Availability

Academic Institutions and Nonprofits only

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pLenti-PGK-c-myc-2A-CDK4(R24C)-GFP-Puro

Plasmid

#83107

Purpose

3rd generation lentiviral expression of C-myc, CDK4(R24C), and GFP separated by 2A self-cleaving peptide

Depositor

Andrew Brooks

Inserts

c-Myc (MYC Human)
CDK4 (CDK4 Human)
eGFP

Use

Lentiviral

Tags

2A self cleaving peptide

Expression

Mammalian

Mutation

R24C and p64 isoform

Promoter

hPGK and hPGK (single transcript)

Available Since

July 3, 2017

Availability

Academic Institutions and Nonprofits only

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pHR-CD86-SmBiT

Plasmid

#223618

Purpose

Lentiviral expression of human CD86 with SmBiT tag in mammalian cells

Depositor

Enfu Hui

Article

Zhao et al Immunity. 2023 Jun 13;56(6):1187-1203.e1

Insert

CD86 (CD86 Human)

Use

Lentiviral; mammalian expression

Tags

SmBiT

Available Since

Sept. 3, 2024

Availability

Academic Institutions and Nonprofits only

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pcDNA3.2-GJA1-S373A

Plasmid

#52879

Purpose

Encodes human Connexin 43 resistant to siRNA with Serine to Alanine mutation at 373

Depositor

Robin Shaw

Article

Smyth et al Traffic. 2014 Mar 10. doi: 10.1111/tra.1

Insert

GJA1 (GJA1 Human)

Expression

Mammalian

Mutation

Serine 373 mutated to Alalnine

Promoter

CMV

Available Since

May 12, 2014

Availability

Academic Institutions and Nonprofits only
pLVX-hSyn-Galpha-s ONE-GO

Plasmid

#214371

Purpose

GPCR/G protein BRET biosensor

Depositor

Mikel Garcia-Marcos

Article

Janicot et al Cell. 2024 Feb 16:S0092-8674(24)00065-5.

Insert

Galpha-s ONE-GO (GNAS Human)

Use

Lentiviral

Promoter

hSyn

Available Since

Feb. 28, 2024

Availability

Industry, Academic Institutions, and Nonprofits

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pCDF-rM1PYK

Plasmid

#220232

Purpose

Inducible expression of rabbit muscle pyruvate kinase isoform 1

Depositor

Aron Fenton, Liskin Swint-Kruse

Article

Swint-Kruse et al Database (Oxford). 2023 May 3;2023:baad0

Insert

Rabbit muscle pyruvate kinase (PKM )

Tags

6xHis-MBP-SUMO

Expression

Bacterial

Promoter

T7

Available Since

Aug. 1, 2024

Availability

Academic Institutions and Nonprofits only

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pcDNA5/FRT-Flag-Cul5

Plasmid

#31984

Purpose

Mammalian expression of Flag-tagged Cul5

Depositor

Joan Conaway

Article

Mahrour et al J Biol Chem. 2008 Mar 21;283(12):8005-13

Insert

Cul5 (CUL5 Human)

Tags

Flag

Expression

Mammalian

Available Since

June 10, 2013

Availability

Academic Institutions and Nonprofits only
MS2_adRNA-MCP_ADAR2 DD_NES

Plasmid

#124706

Purpose

Expresses MS2_adRNA targeting the RAB7A transcript along with the MCP_ADAR2 DD_NES

Depositor

Prashant Mali

Article

Katrekar et al Nat Methods. 2019 Mar;16(3):239-242. doi

Insert

MCP_ADAR2 DD_NES (RAB7A Human)

Use

AAV

Tags

NES

Expression

Mammalian

Promoter

CMV

Available Since

May 14, 2019

Availability

Academic Institutions and Nonprofits only

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CX3C1-Tango

Plasmid

#66258

Purpose

Expression of G protein-coupled receptors for PRESTO-Tango: parallel receptorome expression and screening via transcriptional output, with transcriptional activation following arrestin translocation

Depositor

Bryan Roth

Article

Kroeze et al Nat Struct Mol Biol. 2015 May;22(5):362-

Insert

CX3C1 (CX3CR1 Human)

Tags

FLAG

Expression

Mammalian

Promoter

CMV

Available Since

Sept. 15, 2015

Availability

Academic Institutions and Nonprofits only
MS2_adRNA-MCP_ADAR1 DD_NES

Plasmid

#124630

Purpose

Expresses MS2_adRNA targeting the RAB7A transcript along with MCP_ADAR1 DD_NES

Depositor

Prashant Mali

Article

Katrekar et al Nat Methods. 2019 Mar;16(3):239-242. doi

Insert

MCP_ADAR1 DD_NES (RAB7A Human)

Use

AAV

Tags

NES

Expression

Mammalian

Promoter

CMV

Available Since

May 14, 2019

Availability

Academic Institutions and Nonprofits only

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pRRLHygro-pEF1a-p53ash-mKate2-splitmVenusC

Plasmid

#69583

Purpose

Expresses p53 tagged with mKate2 and split C-term mVenus

Depositor

Galit Lahav

Article

Gaglia et al Mol Syst Biol. 2014 Oct 24;10:753. doi:

Insert

p53 (TP53 Human)

Use

Lentiviral

Tags

mKate2 and split mVenus - C term

Expression

Mammalian

Mutation

7 silent mutations in p53 DNA to prevent targetin…

Promoter

EF1alpha

Available Since

Nov. 14, 2017

Availability

Academic Institutions and Nonprofits only

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mCerulean3-Bcl-2-s2193

Plasmid

#166750

Purpose

Express mCerulean3 fused to the N-terminus of the Bcl-2 family protein, Bcl-2 in a plasmid with Blasticidin resistance. Used to make stable BMK-DKO cell lines.

Depositor

David Andrews

Article

Liu et al Elife. 2019 Mar 12;8. pii: 37689. doi: 1

Insert

Bcl-2 (BCL2 Human)

Tags

mCerulean3

Expression

Mammalian

Promoter

Human ferritin

Available Since

Oct. 4, 2021

Availability

Academic Institutions and Nonprofits only

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