We narrowed to 13,020 results for: cDNA

1. pcDNA5/FRT/TO-GAlphaQ-RLuc8

   Plasmid

   #140982

   Purpose

   Encodes a G alpha subunit (GNAQ) containing RLuc8 as an optimal component of a BRET2 biosensor for studying heterotrimeric G protein dissociation

   Depositor

   Bryan Roth

   Article

   Olsen et al Nat Chem Biol. 2020 May 4. pii: 10.1038/

   Insert

   GAlphaQ-RLuc8 (GNAQ Human)
   

   Use

   Luciferase

   Tags

   Expression

   Mammalian

   Mutation

   RLuc8 and flanking SGGGGS linkers have been inser…

   Promoter

   CMV

   Available since

   June 26, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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2. pcDNA3-sACt-mCherry-NLS

   Plasmid

   #138214

   Purpose

   Encodes residues 1-469 of rat soluble adenylyl cyclase (ADCY10) fused to mCherry; nuclear targeted.

   Depositor

   Jin Zhang

   Article

   Sample et al Nat Chem Biol. 2012 Feb 26;8(4):375-82.

   Insert

   sACt-mCherry-NLS (Adcy10 Rat)
   

   Use

   Tags

   6xHIS - T7 tag (gene 10 leader) - Xpress (TM) tag…

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   March 24, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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3. pcDNA3.1/Myc-His(-)-MSulf-1

   Plasmid

   #13007

   Depositor

   Steven Rosen

   Article

   Morimoto-Tomita et al J Biol Chem. 2002 Dec 20. 277(51):49175-

   Insert

   mouse sulfatase 1 (Sulf1 Mouse)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Oct. 30, 2006

   Availability

   Academic Institutions and Nonprofits only

4. pCDNA-3xFLAG-NLS-TPP1

   Plasmid

   #53585

   Purpose

   Mammalian expression of 3xFLAG tagged, nuclear localized TPP1, N-terminal.

   Depositor

   Steven Artandi

   Article

   Zhong et al Cell. 2012 Aug 3;150(3):481-94. doi: 10.

   Insert

   TPP1 (ACD Human)
   

   Use

   Tags

   3xFLAG and NLS

   Expression

   Mammalian

   Mutation

   Silent mutation to eliminate PacI site

   Promoter

   CMV

   Available since

   June 9, 2014

   Availability

   Academic Institutions and Nonprofits only

5. pcDNA5/FRT/TO-GAlphai3-RLuc8

   Plasmid

   #140975

   Purpose

   Encodes a G alpha subunit (GNAI3) containing RLuc8 as an optimal component of a BRET2 biosensor for studying heterotrimeric G protein dissociation

   Depositor

   Bryan Roth

   Article

   Olsen et al Nat Chem Biol. 2020 May 4. pii: 10.1038/

   Insert

   GAlphai3-RLuc8 (GNAI3 Human)
   

   Use

   Luciferase

   Tags

   Expression

   Mammalian

   Mutation

   RLuc8 and flanking SGGGGS linkers have been inser…

   Promoter

   CMV

   Available since

   June 26, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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6. pcDNA3.1+(rInsp)-Ins-GLuc

   Plasmid

   #89928

   Purpose

   Expresses Insulin-GLuc from the rat insulin promoter. Gaussia Luc is inserted within the C-peptide and is co-secreted with insulin.

   Depositor

   Melanie Cobb

   Article

   Kalwat et al ACS Sens. 2016 Oct 28;1(10):1208-1212. E

   Insert

   human insulin-Gaussia-Luciferase (INS Synthetic, Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   DNA sequence for Gaussia luciferase was humanized…

   Promoter

   rat Insulin II promoter

   Available since

   Sept. 4, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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7. GeNL(Ca2+)_60/pcDNA3

   Plasmid

   #85207

   Purpose

   luminescent reporter for calcium signaling

   Depositor

   Takeharu Nagai

   Article

   Suzuki et al Nat Commun. 2016 Dec 14;7:13718. doi: 10

   Insert

   GeNL(Ca2+)_60
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   March 19, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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8. pcDNA3.1(+)/AUG-nLuc-3XFLAG

   Plasmid

   #127299

   Purpose

   Express 3xFLAG tagged nanoLuciferase (nLuc) from AUG start codon

   Depositor

   Jeremy Wilusz

   Article

   Kearse et al Genes Dev. 2019 Jun 6. pii: gad.324715.1

   Insert

   nanoLuciferase
   

   Use

   Tags

   3xFLAG

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   June 21, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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9. 1478 pcDNA3 flag HA 14-3-3 beta

   Plasmid

   #8999

   Depositor

   William Sellers

   Insert

   14-3-3 beta (YWHAB Human)
   

   Use

   Tags

   HA and flag

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   April 20, 2006

   Availability

   Academic Institutions and Nonprofits only

10. Human NCKX6-FlagTag (pcDNA3.1+)

    Plasmid

    #75217

    Purpose

    Mammalian expression of SLC8B1

    Depositor

    Jonathan Lytton

    Article

    Cai et al J Biol Chem. 2004 Feb 13;279(7):5867-76.

    Insert

    SLC8B1 (SLC8B1 Human)
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    June 21, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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11. pcDNA3-SEP-Glur2 (MCA10)

    Plasmid

    #64941

    Purpose

    mammalian expression of rat GluR2

    Depositor

    Jeremy Henley

    Article

    Ashby et al J Neurosci. 2004 Jun 2;24(22):5172-6.

    Insert

    GluR2 (Gria2 Rat)
    

    Use

    Tags

    SEP (superecliptic pHluorin)

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    June 16, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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12. pcDNA3.2/V5 hsa-mir-150

    Plasmid

    #26310

    Depositor

    David Bartel

    Article

    Chiang et al Genes Dev. 2010 May 15. 24(10):992-1009.

    Insert

    hsa-mir-150 (MIR150 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Sept. 29, 2010

    Availability

    Academic Institutions and Nonprofits only

    

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13. pcDNA3.1-Hygro(+)-PSTCD-cGAS

    Plasmid

    #102605

    Purpose

    Mammalian expression of cGAS fused to PSTCD tag

    Depositor

    Nicolas Manel

    Article

    Gentili et al Science. 2015 Sep 11;349(6253):1232-6. d

    Insert

    cGAS (CGAS Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Jan. 18, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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14. pcDNA-GFP-EphrinB3-Flag

    Plasmid

    #155012

    Purpose

    Expresses N-terminally GFP-tagged and C-terminally Flag-tagged EphrinB3 from pcDNA3.1

    Depositor

    Sinisa Urban

    Article

    Gandhi et al Cell Chem Biol. 2020 Aug 31. pii: S2451-

    Insert

    EphrinB3 (EPHB3 Human)
    

    Use

    Tags

    Flag, GFP, and signal peptide (residues 1 to 32) …

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Nov. 2, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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15. pcDNA3.1-Flag-PGC-1alpha1

    Plasmid

    #45501

    Depositor

    Bruce Spiegelman, Jorge Ruas

    Article

    Ruas et al Cell. 2012 Dec 7;151(6):1319-31. doi: 10

    Insert

    PGC-1alpha Isoform1 (Ppargc1a Mouse)
    

    Use

    Tags

    6xHis and Flag

    Expression

    Mammalian

    Mutation

    Q339K relative to NM_008904

    Promoter

    Available since

    Aug. 9, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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16. pcDNA3-Tsapphire-StagRFP-SrcAR

    Plasmid

    #173018

    Purpose

    Long-Stokes-shift green-red FRET-based Src activity reporter for multiplexed imaging applications.

    Depositor

    Jin Zhang

    Article

    Mo et al Nat Commun. 2020 Apr 15;11(1):1848. doi:

    Insert

    Tsapphire-StagRFP-SrcAR
    

    Use

    Tags

    6xHIS - T7 tag (gene 10 leader) - Xpress (TM) tag…

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Oct. 7, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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17. pCDNA3 Flag MKK7B2Jnk1a1(APF)

    Plasmid

    #19730

    Depositor

    Roger Davis

    Article

    Lei et al Mol Cell Biol. 2002 Jul . 22(13):4929-42

    Insert

    MKK7B2Jnk1a1(APF) (MAPK8, Map2k7 Mouse, Human)
    

    Use

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    replaced dual phosporylation motif Thr(1959)-Pro-…

    Promoter

    Available since

    March 31, 2009

    Availability

    Academic Institutions and Nonprofits only

    

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18. pcDNA3.1 SARS-CoV-2 S D614

    Plasmid

    #158074

    Purpose

    Expresses SARS-CoV-2 spike protein

    Depositor

    Jeremy Luban

    Article

    Yurkovetskiy et al Cell. 2020 Sep 15. pii: S0092-8674(20)31

    Insert

    SARS-CoV-2 spike (S )
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 28, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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19. pcDNA3puro-mGFP-FMR1iso1-G266E

    Plasmid

    #225468

    Purpose

    Express mEGFP-fusion protein of FMR1 isoform 1 (G266E)

    Depositor

    Christine Mayr

    Article

    Chen et al Cell. 5 August 2024. doi: 10.1016/j.cell

    Insert

    FMR1 isoform 1 (FMR1 Human)
    

    Use

    Tags

    mEGFP

    Expression

    Mammalian

    Mutation

    G266E

    Promoter

    CMV

    Available since

    Jan. 16, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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20. pcDNA3puro-mGFP-FMR1iso1-I304N

    Plasmid

    #225463

    Purpose

    Express mEGFP-fusion protein of FMR1 isoform 1 (I304N)

    Depositor

    Christine Mayr

    Article

    Chen et al Cell. 5 August 2024. doi: 10.1016/j.cell

    Insert

    FMR1 isoform 1 (FMR1 Human)
    

    Use

    Tags

    mEGFP

    Expression

    Mammalian

    Mutation

    I304N

    Promoter

    CMV

    Available since

    Oct. 10, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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