We narrowed to 24,475 results for: crispr

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Sc++

Plasmid

#155011

Purpose

Expresses the codon-optimized, engineered Sc++ Cas9 nuclease in mammalian cells

Depositor

Joseph Jacobson

Article

Chatterjee et al Nat Biotechnol. 2020 May 11. pii: 10.103

Insert

Sc++

Expression

Mammalian

Mutation

T1227K, Loop Substitution from S. anginosus

Promoter

EF1ɑ

Available Since

Sept. 11, 2020

Availability

Academic Institutions and Nonprofits only

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ND6-DdCBE-right side TALE

Plasmid

#157841

Purpose

expresses right-side TALE–DddAtox half of ND6-DdCBE in mammalian cells

Depositor

David Liu

Article

Mok et al Nature. 2020 Jul 8. pii: 10.1038/s41586-

Insert

COX8A MTS–3xFLAG–ND6 right TALE–G1397 DddA-C–UGI–ATP5B 3'UTR

Expression

Mammalian

Available Since

Aug. 4, 2020

Availability

Academic Institutions and Nonprofits only

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ND6-DdCBE-left side TALE

Plasmid

#157842

Purpose

expresses left-side TALE–DddAtox half of ND6-DdCBE in mammalian cells

Depositor

David Liu

Article

Mok et al Nature. 2020 Jul 8. pii: 10.1038/s41586-

Insert

SOD2 MTS–3x-HA–ND6 left tale–G1397 DddA-N–UGI–SOD2 3'UTR

Expression

Mammalian

Available Since

Aug. 4, 2020

Availability

Academic Institutions and Nonprofits only

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3XFLAG-VP64-SadCas9-NLS-VP64

Plasmid

#135338

Purpose

3xFLAG 2xVP64 SadCas9 for single guide cloning

Depositor

Ronald Cohn

Article

Kemaladewi et al Nature. 2019 Aug;572(7767):125-130. doi:

Insert

SadCas9

Tags

3xFLAG-VP64-SV40 NLS and NLS-VP64

Expression

Mammalian

Mutation

D10A and N580A

Promoter

CMV

Available Since

March 11, 2020

Availability

Academic Institutions and Nonprofits only

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G9A[SET]-dCas9

Plasmid

#100089

Purpose

Catalytic domain [SET] of human G9A fused to N-terminus of dCas9; pCDNA3 vector backbone, mammalian expression

Depositor

David Segal

Article

O'Geen et al Nucleic Acids Res. 2017 Sep 29;45(17):99

Insert

G9A (EHMT2 Human)

Use

CRISPR

Tags

3XFLag-NLS-G9A[SET]-dCas9-NLS

Expression

Mammalian

Mutation

G9A[SET] includes aa 829-1209

Promoter

CMV

Available Since

Oct. 30, 2017

Availability

Academic Institutions and Nonprofits only

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pAAV MCS mCherry-KASH

Plasmid

#139654

Purpose

Cloning template for AAV-based ORANGE knock-in constructs

Depositor

Harold MacGillavry

Article

Willems et al PLoS Biol. 2020 Apr 10;18(4):e3000665. d

Type

Empty backbone

Use

AAV

Expression

Mammalian

Promoter

U6

Available Since

June 8, 2020

Availability

Academic Institutions and Nonprofits only

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pAAV-EFS-CjCas9-eGFP-HIF1a

Plasmid

#137929

Purpose

Expression of cjCas9 with Hif1a targeting sgRNA

Depositor

Jin-Soo Kim

Article

Kim et al Nat Commun. 2017 Feb 21;8:14500. doi: 10

Insert

CjCas9

Use

AAV and CRISPR

Tags

SV40NLS-HA-T2A-EGFP

Promoter

EF-1alpha

Available Since

March 4, 2020

Availability

Academic Institutions and Nonprofits only

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pAW90.dCas9-YY1

Plasmid

#104373

Purpose

Expresses dCas9-YY1

Depositor

Richard Young

Article

Weintraub et al Cell. 2017 Dec 14;171(7):1573-1588.e28.

Insert

dCas9-YY1

Available Since

Jan. 8, 2018

Availability

Academic Institutions and Nonprofits only

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pHR-UCOE-SFFV-Zim3-dCas9-P2A-BFP

Plasmid

#188767

Purpose

dCas9 with an N-term Zim3 KRAB-NLS fusion, a C-term HA-2xNLS

Depositor

Marco Jost, Jonathan Weissman

Article

Replogle et al eLife. 2022 Dec 28;11:e81856. doi: 10.75

Insert

Zim3-dCas9

Use

Lentiviral

Tags

HA-2xNLS and Zim3 KRAB-NLS fusion

Promoter

SFFV

Available Since

Oct. 20, 2022

Availability

Academic Institutions and Nonprofits only

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WN10150

Plasmid

#80443

Purpose

Expresses dead (908A) AsCpf1

Depositor

Ervin Welker

Article

Toth et al Nucleic Acids Res. 2018 Sep 20. pii: 510

Insert

nuclease dead (908A) AsCpf1

Tags

NLS-3xHA

Expression

Mammalian

Mutation

nuclease dead (D908A)

Available Since

Aug. 29, 2016

Availability

Academic Institutions and Nonprofits only

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pCFD5-frame_selector_0,1,2

Plasmid

#131152

Purpose

Drosophila expression of frame selector sgRNAs 0,1, and 2 that target the CRISPaint site for homology-independent knock-in

Depositor

Norbert Perrimon

Article

Bosch et al Genetics. 2019 Nov 4. pii: genetics.119.

Insert

CRISPaint frame selector sgRNAs 0,1,2

Use

CRISPR

Expression

Insect

Promoter

dU6:3

Available Since

Sept. 6, 2019

Availability

Academic Institutions and Nonprofits only

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bRA89

Plasmid

#100950

Purpose

Cas9 driven by PGK1 promoter. gRNA can be cloned into the BplI sites. HPH marker

Depositor

James Haber

Article

Anand et al Nature. 2017 Apr 20;544(7650):377-380. d

Type

Empty backbone

Use

CRISPR and Synthetic Biology

Expression

Yeast

Available Since

Sept. 11, 2017

Availability

Academic Institutions and Nonprofits only

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PDX1-2A-eGFP

Plasmid

#66964

Purpose

PDX1-eGFP mini-vector donor for integration of the eGFP reporter into the PDX1 locus

Depositor

Danwei Huangfu

Article

Zhu et al Stem Cell Reports. 2015 May 28. pii: S22

Insert

pancreatic and duodenal homeobox 1 (PDX1 Human)

Use

Unspecified

Tags

2A-eGFP

Available Since

July 24, 2015

Availability

Academic Institutions and Nonprofits only

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Lenti-sgp53/Cre

Plasmid

#89646

Purpose

Expresses a p53-targeting gRNA and Cre-recombinase

Depositor

Monte Winslow

Article

Rogers et al Nat Methods. 2017 May 22. doi: 10.1038/n

Insert

gRNA targeting p53 (Trp53 Mouse)

Use

Cre/Lox and Lentiviral

Expression

Mammalian

Available Since

July 19, 2017

Availability

Academic Institutions and Nonprofits only

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pMOD_A1110

Plasmid

#91031

Purpose

Module A, Promoter: ZmUbi, Gene: TaCas9, Terminator: HSP

Depositor

Daniel Voytas

Article

Cermak et al Plant Cell. 2017 May 18. pii: tpc.00922.

Insert

TaCas9

Use

CRISPR

Promoter

ZmUbi

Available Since

July 7, 2017

Availability

Academic Institutions and Nonprofits only

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pJMP1339

Plasmid

#119271

Purpose

for cloning new sgRNAs

Depositor

Carol Gross, Jason Peters, Oren Rosenberg

Article

Peters et al Nat Microbiol. 2019 Jan 7. pii: 10.1038/

Insert

dcas9

Expression

Bacterial

Mutation

D10A and H840A

Available Since

Jan. 8, 2019

Availability

Academic Institutions and Nonprofits only

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pCMV-eNme2-T.1-ABE8e

Plasmid

#185668

Purpose

Expresses eNme2-T.1-ABE8e in mammalian cells

Depositor

David Liu

Article

Huang et al Nat Biotechnol. 2022 Sep 8. pii: 10.1038

Insert

ecTadA(8e)-neNme2-T.2

Use

CRISPR

Expression

Mammalian

Mutation

Nme2Cas9 D16A/E47K/V68M/T123A/D152G/E154K/T396A/H…

Available Since

Sept. 26, 2022

Availability

Academic Institutions and Nonprofits only

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pCAG-BRCA1

Plasmid

#119007

Purpose

Mammalian expression of human BRCA1 and BFP

Depositor

Ralf Kuehn

Article

Bashir et al BMC Biotechnol. 2020 Oct 23;20(1):57. do

Insert

BRCA1 (BRCA1 Human)

Expression

Mammalian

Promoter

CAG

Available Since

Oct. 28, 2020

Availability

Academic Institutions and Nonprofits only

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pCMV-SpRY-HF1-ABE8e

Plasmid

#185670

Purpose

Expresses SpRY-HF1-ABE8e in mammalian cells

Depositor

David Liu

Article

Huang et al Nat Biotechnol. 2022 Sep 8. pii: 10.1038

Insert

ecTadA(8e)-nSpRY-HF1

Use

CRISPR

Expression

Mammalian

Mutation

SpRY-HF1 D10A

Available Since

Sept. 16, 2022

Availability

Academic Institutions and Nonprofits only

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pCB591

Plasmid

#141096

Purpose

E. coli-Lactobacilli shuttle vector for recombineering template cloning

Depositor

Chase Beisel

Article

Leenay et al Biotechnol J. 2019 Mar;14(3):e1700583. d

Type

Empty backbone

Expression

Bacterial

Available Since

May 6, 2020

Availability

Academic Institutions and Nonprofits only

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