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We narrowed to 25,138 results for: crispr

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Showing: 4261 - 4280 of 25138 results

  8. pU6T7

    Plasmid
    #71462
    Purpose
    Expresses gRNA from hybrid human U6/T7 promoter for both cellular expression and in vitro transcription from same promoter (2 Gs at initiation)
    Depositor
    Willie Mark
    Publication
    Romanienko et al PLoS One. 2016 Feb 5;11(2):e0148362. doi
    Type
    Empty backbone
    Use
    CRISPR
    Expression
    Bacterial and Mammalian
    Promoter
    human U6/phage T7
    Available since
    Feb. 9, 2016
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    71462-plasmid-map-sequence-id-128762

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  9. pSaGuide

    Plasmid
    #64710
    Purpose
    Cloning and expression of Staphylococcus aureus guide RNA
    Depositor
    Kiran Musunuru
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    Staphylococcus aureus guide RNA
    Use
    CRISPR
    Expression
    Mammalian
    Promoter
    hU6
    Available since
    May 14, 2015
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    64710-plasmid-map-sequence-id-108923

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  11. pUBC_V5_MS2-mCherry

    Plasmid
    #68420
    Purpose
    Transient expression of humanized MS2 (V75E/A81G), fused to mCherry in mammalian cells, under a UBC promoter
    Depositor
    John Rinn
    Publication
    Shechner et al Nat Methods. 2015 Jul;12(7):664-70. doi:
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    MS2
    Use
    Synthetic Biology
    Tags
    V5 and mCherry
    Expression
    Mammalian
    Mutation
    MS2: V75E/A81G
    Promoter
    human UbC
    Available since
    Oct. 5, 2015
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    68420-plasmid-map-sequence-id-511194

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  12. 3xFLAG-dCas9/p-bacteria

    Plasmid
    #64325
    Purpose
    Expresses 3xFLAG-dCas9 in E. coli in a tetracycline-dependent manner for enChIP analysis to purify specific genomic regions of interest.
    Depositor
    Hodaka Fujii
    Publication
    Fujita et al BMC Res Notes. 2018 Jun 14;11(1):387. do
    Insert
    3xFLAG-dCas9
    Use
    CRISPR; Tetracycline-inducible
    Tags
    3xFLAG
    Expression
    Bacterial
    Mutation
    D10A, H840A
    Promoter
    pLtetO-1
    Available since
    Aug. 3, 2015
    Availability
    Academic Institutions and Nonprofits only
    64325-plasmid-map-sequence-id-107969

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  18. VVT1

    Plasmid
    #65779
    Purpose
    The Joung Lab recommends using BPK2660 (Addgene plasmid 70709) instead of VVT1 as guide RNAs cloned into BPK2660 are more effective than this plasmid. Human expression plasmid for S. aureus Cas9 sgRNA
    Depositor
    Keith Joung
    Publication
    Kleinstiver et al Nature. 2015 Jun 22. doi: 10.1038/nature
    Insert
    SaCas9 gRNA backbone, without spacer sequence
    Use
    CRISPR
    Expression
    Mammalian
    Promoter
    U6
    Available since
    June 22, 2015
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    65779-plasmid-map-sequence-id-112660

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  19. pMB1610_pRR-Puro

    Plasmid
    #65853
    Purpose
    Puromycin-based homologous recombination-dependent reporter for genome editing with TALENs or CRISPR/Cas9
    Depositor
    Marc Bühler
    Publication
    Flemr et al Cell Rep. 2015 Jul 28;12(4):709-16. doi:
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    split puromycin N-acetyltransferase coding sequence
    Use
    CRISPR and TALEN
    Expression
    Mammalian
    Promoter
    SV40
    Available since
    Aug. 11, 2015
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    65853-plasmid-map-sequence-id-112015

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Showing: 4261 - 4280 of 25138 results