We narrowed to 9,742 results for: Tec

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pEx_Hsp70::attBmut

Plasmid

#48878

Purpose

PhiC31 exchange vector with the Hsp70 promoter driving tagRFPt from the landing site. Template for enhancer tests.

Depositor

Joachim Wittbrodt

Article

Kirchmaier et al Development. 2013 Sep 18.

Type

Empty backbone

Available Since

Dec. 18, 2013

Availability

Academic Institutions and Nonprofits only

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pRDA_722

Plasmid

#216077

Purpose

CMV-driven, lentiCRISPRv2-based destination vector (preferred over pRDA_512)

Depositor

John Doench

Article

McGee et al Cell Genom. 2024 Mar 13;4(3):100519. doi

Has Service

Cloning Grade DNA

Type

Empty backbone

Use

CRISPR and Lentiviral; Destination

Available Since

April 29, 2024

Availability

Academic Institutions and Nonprofits only

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pJBL728

Plasmid

#140398

Purpose

Expresses CsoR-regulated 3WJdB RNA aptamer driven by T7 promoter with the csoO sequence 5BP downstream from the promoter

Depositor

Julius Lucks

Article

Jung et al Nat Biotechnol. 2020 Jul 6. pii: 10.1038

Insert

T7-csoO-3WJdB-T

Use

Synthetic Biology

Available Since

July 8, 2020

Availability

Academic Institutions and Nonprofits only

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pCAG-VHC

Plasmid

#112618

Purpose

expression plasmid with CAG promoter driving multicistronic cassette H2BVenus_p2A_HygroR_p2A_CreERt2

Depositor

Stefan Heller

Article

Hartman et al Dev Biol. 2018 Sep 1. pii: S0012-1606(18

Inserts

H2B-Venus (H2BC3 Aequorea victoria, Human)
Hygrophosphotransferase
Cre-ERT2 fusion protein

Tags

Venus

Expression

Mammalian

Promoter

CAG

Available Since

Sept. 13, 2018

Availability

Academic Institutions and Nonprofits only

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pTS1106_Tier3(Lenti)

Plasmid

#169661

Purpose

Tier-3 vector for stable integration of up to three cassettes via lentiviral transduction (PCMV-5'LTR-Psi-gag-env-A1::A2::A3-WPRE-3'LTR)

Depositor

Martin Fussenegger

Article

Haellman et al Metab Eng. 2021 Jul;66:41-50. doi: 10.10

Type

Empty backbone

Expression

Mammalian

Available Since

June 28, 2022

Availability

Academic Institutions and Nonprofits only

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A1-20B1 (P001)

Plasmid

#161820

Purpose

This plasmid carries Yn situ preamplifier A1-20B1 sequence.

Depositor

C. Ron Yu

Article

Wu et al Cell Rep Methods. 2022 Apr 18;2(4):10020

Insert

A1-20B1

Use

Synthetic Biology

Available Since

May 13, 2022

Availability

Academic Institutions and Nonprofits only

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pJBL704

Plasmid

#140374

Purpose

Expresses TetR-regulated 3WJdB RNA aptamer driven by T7 promoter with the tetO sequence 4BP dowsntream from the promoter

Depositor

Julius Lucks

Article

Jung et al Nat Biotechnol. 2020 Jul 6. pii: 10.1038

Insert

T7-4BP-tetO-3WJdB-T

Use

Synthetic Biology

Available Since

July 8, 2020

Availability

Academic Institutions and Nonprofits only

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pAW091

Plasmid

#113253

Purpose

pUC19 backbone with S. pyogenes CRISPR array

Depositor

Jennifer Doudna

Article

Wright et al Nat Struct Mol Biol. 2016 Oct;23(10):876

Insert

S. pyogenes CRISPR array

Use

Integration substrate

Promoter

NA

Available Since

Sept. 7, 2018

Availability

Academic Institutions and Nonprofits only

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pExo1

Plasmid

#53357

Purpose

Expresses beta-lactamase for the degradation of beta-lactam antibiotics and tags it with hlyAs (transporter gene) in preparation for secretion from the cell.

Depositor

Lingchong You

Article

Pai et al Proc Natl Acad Sci U S A. 2012 Nov 27;10

Inserts

BlaMs
hlyAs

Promoter

p(lac/ara-1)

Available Since

July 15, 2014

Availability

Academic Institutions and Nonprofits only

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BCJJ358

Plasmid

#117505

Purpose

RPS5a:Cas9:E9 Golden Gate Level 1 Position 2 reverse

Depositor

Jonathan D Jones

Article

Castel et al PLoS One. 2019 Jan 9;14(1):e0204778. doi

Insert

BpiI:tagt:RPS5a:Cas9_4:E9:ttgc:BpiI

Use

CRISPR

Expression

Plant

Promoter

AtRPS5a

Available Since

Oct. 17, 2018

Availability

Academic Institutions and Nonprofits only

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pBL132

Plasmid

#104333

Purpose

Expresses taz from a LacI regulated promoter under inducible IPTG control.

Depositor

Jeffrey Tabor

Article

Landry et al Nat Commun. 2018 Apr 12;9(1):1433. doi:

Insert

Taz

Expression

Bacterial

Promoter

PtacI

Available Since

April 17, 2018

Availability

Academic Institutions and Nonprofits only

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CFP-FKBP

Plasmid

#162433

Purpose

for use in CID (chemically induced dimerization) or CIT (chemically induced trimerization) system

Depositor

Takanari Inoue

Article

Wu et al Nat Methods. 2020 Sep;17(9):928-936. doi

Insert

CFP-FKBP

Expression

Mammalian

Promoter

CMV

Available Since

Dec. 16, 2020

Availability

Academic Institutions and Nonprofits only

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Opie2-NeoR

Plasmid

#131614

Purpose

Expresses NeoR and dsRed.

Depositor

Omar Akbari

Article

Kandul et al Nat Commun. 2020 Apr 30;11(1):2106. doi:

Inserts

NeoR
dsRed

Expression

Insect

Promoter

Opie2 and hr5Ie1

Available Since

May 7, 2020

Availability

Academic Institutions and Nonprofits only

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pColE1_sgRNA_pos8_AmpR

Plasmid

#119151

Purpose

Encodes sgRNA targeting position 8 in pColE1_70a_deGFP_KanR

Depositor

Vincent Noireaux

Article

Collias et al Synth Biol (Oxf). 2019 Jan 21;4(1):ysz00

Insert

sgRNA targeting position 8 in pColE1_70a_deGFP_KanR

Use

Crispr

Available Since

Nov. 28, 2018

Availability

Academic Institutions and Nonprofits only

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POT2-RFP

Plasmid

#65318

Purpose

receiving vector of standard transcription units(TUs) in YeastFab work, useful for further assembly

Depositor

Junbiao Dai

Article

Guo et al Nucleic Acids Res. 2015 May 8. pii: gkv4

Insert

RFP

Use

Synthetic Biology

Available Since

Feb. 26, 2016

Availability

Academic Institutions and Nonprofits only

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SNAP-EF(N)-5nmSAH-GFP(1-10)

Plasmid

#61659

Purpose

5nm SAH scaffold with EF Hand and split GFP attachment sites

Depositor

Kristen Verhey

Article

Norris et al J Cell Biol. 2014 Nov 10;207(3):393-406.

Insert

SNAP-EF(N)-5nmSAH-GFP(1-10)

Expression

Mammalian

Available Since

March 11, 2015

Availability

Academic Institutions and Nonprofits only

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SNAP-EF(N)-10nmSAH-GFP(1-10)

Plasmid

#61660

Purpose

10nm SAH scaffold with EF Hand and split GFP attachment sites

Depositor

Kristen Verhey

Article

Norris et al J Cell Biol. 2014 Nov 10;207(3):393-406.

Insert

SNAP-EF(N)-10nmSAH-GFP(1-10)

Expression

Mammalian

Available Since

March 11, 2015

Availability

Academic Institutions and Nonprofits only

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pMSCVII-AM

Plasmid

#162751

Purpose

Retroviral (MSCV) expression of ametrine fluorescent protein

Depositor

Maki Nakayama

Article

Landry et al Bio Protoc. 2021 Jan 20;11(2):e3883. doi

Insert

ametrine

Use

Retroviral

Expression

Mammalian

Available Since

Jan. 7, 2021

Availability

Academic Institutions and Nonprofits only

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BCJJ344

Plasmid

#117503

Purpose

UBI10:Cas9:E9 Golden Gate Level 1 Position 2 reverse

Depositor

Jonathan D Jones

Article

Castel et al PLoS One. 2019 Jan 9;14(1):e0204778. doi

Insert

BpiI:tagt:UBI10:Cas9-3(intron):E9:ttgc:BpiI

Use

CRISPR

Expression

Plant

Promoter

AtUBI10

Available Since

Nov. 26, 2018

Availability

Academic Institutions and Nonprofits only

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BCJJ340

Plasmid

#117502

Purpose

UBI10:Cas9:E9 Golden Gate Level 1 Position 2

Depositor

Jonathan D Jones

Article

Castel et al PLoS One. 2019 Jan 9;14(1):e0204778. doi

Insert

BpiI:GCAA:UBI10:Cas9-3(intron):E9:ACTA:BpiI

Use

CRISPR

Expression

Plant

Promoter

AtUBI10

Available Since

Oct. 17, 2018

Availability

Academic Institutions and Nonprofits only

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