We narrowed to 22,617 results for: Ide
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Plasmid#207376PurposeExpression of increased-fidelity (i.e. high-fidelity) SpCas9 variant in bacterial cellsDepositorInsertHiFi SpCas9
UseCRISPRTags6xHisExpressionBacterialMutationR691APromoterT7Available SinceSept. 25, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAAV-GfaABC1D-GRAB_Ado1.0med
Plasmid#153289PurposeExpress the medium-affinity adenosine sensor GRAB_Ado1.0med in astrocytesDepositorInsertAdenosine sensor GRAB_Ado1.0med (medium affinity)
UseAAVPromoterGfaABC1DAvailable SinceAug. 3, 2020AvailabilityAcademic Institutions and Nonprofits only -
pDEST14-SpyCatcher002 EQ
Plasmid#102830PurposeBacterial expression of unreactive version of SpyCatcher002DepositorInsertSpyCatcher002 EQ
TagsGSSGS, His6, and TEV cleavage siteExpressionBacterialMutationE77Q mutation prevents isopeptide bond formationPromoterT7Available SinceDec. 20, 2017AvailabilityAcademic Institutions and Nonprofits only -
Prolactin-CD4d3+4-bio
Plasmid#73123PurposeEXPRESs plasmid for human erythrocyte surface proteins encoding Prolactin with rat CD4d3+4-bioDepositorAvailable SinceMarch 29, 2016AvailabilityAcademic Institutions and Nonprofits only -
p221z-erTagRFP
Plasmid#71266PurposeEntry clone containing ER-localized TagRFP. Can be used to construct transcriptional reporters. For use in plants and compatible with the MultiSite Gateway systemDepositorInsertTagRFP
UseGateway CloningTagsER retention signal HDEL and signal peptide (ER)…Available SinceJan. 6, 2016AvailabilityAcademic Institutions and Nonprofits only -
p221z-erVenusYFP
Plasmid#71265PurposeEntry clone containing ER-localized Venus. Can be used to construct transcriptional reporters. For use in plants and compatible with the MultiSite Gateway systemDepositorInsertVenusYFP
UseGateway CloningTagsER retention signal HDEL and signal peptide (ER)…Available SinceJan. 6, 2016AvailabilityAcademic Institutions and Nonprofits only -
mCherry-Galectin-C-18
Plasmid#62745PurposeLocalization: Membrane Receptor, C1 Cloning Vector, Excitation:587, Emission:610DepositorAvailable SinceMarch 10, 2015AvailabilityAcademic Institutions and Nonprofits only -
pRK5-mWnt2
Plasmid#42274DepositorInsertWnt2 (Wnt2 Mouse)
ExpressionMammalianAvailable SinceJune 21, 2013AvailabilityAcademic Institutions and Nonprofits only -
A3Bi-ctd-Cas9n-UGI-NLS
Plasmid#109426PurposeExpresses the C-terminal catalytic domain of human APOBEC3B containing an L1 intron fused to Cas9n, Uracil DNA Glycosylase Inhibitor, with a nuclear localization signal.DepositorInsertApolipoprotein B mRNA Editing Enzyme, Catalytic Polypeptide-like 3B C-terminal domain (APOBEC3B Human)
UseCRISPRExpressionMammalianMutationInsertion of an L1 intron into the coding sequenc…PromoterCMVAvailable SinceJuly 2, 2018AvailabilityAcademic Institutions and Nonprofits only -
pWZL Neo Myr Flag GCK
Plasmid#20492DepositorAvailable SinceOct. 27, 2009AvailabilityAcademic Institutions and Nonprofits only -
pEX-CFP-STIM1-deltaK
Plasmid#18860DepositorInsertSTIM1 (STIM1 Human)
TagsCFPExpressionMammalianMutationStop codon at amino acid position 671 of STIM1. C…Available SinceOct. 15, 2008AvailabilityAcademic Institutions and Nonprofits only -
nos-Cas9.874Z1
Plasmid#112685PurposeExpress hSpCas9 under nanos promoterDepositorInserthSpCas9
Tags3x FLAG, EGFP, and NLSExpressionInsectMutationHumanized Cas9 bacterial sequencePromoternanosAvailable SinceJan. 8, 2019AvailabilityAcademic Institutions and Nonprofits only -
pX330-GFP-SFPQ
Plasmid#97084PurposeEncodes an sgRNA that creates a DSB at the promoter of human SFPQ gene.DepositorInsertsgRNA GFP-SFPQ
UseCRISPRPromoterU6Available SinceJuly 31, 2017AvailabilityAcademic Institutions and Nonprofits only -
pBP-T7_RBS-His6-Thrombin
Plasmid#72979PurposeT7 promoter, RBS, His6-tag and thrombin cleave site - derived from pET15b (5' CTAT/3' CATA) fusion)DepositorInsertT7+RBS+His6+thrombin
UseSynthetic BiologyMutationCAT gene - C435G (nucleotide) - silent mutagenesi…Available SinceJuly 6, 2016AvailabilityAcademic Institutions and Nonprofits only -
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pWaldo-GFPe_PepTSo
Plasmid#58334PurposeExpresses the POT family transporter from Shewanella oniedensis in E. coli as a C-terminally tagged GFP his tagged proteinDepositorInsertPepTso
TagsGFP-HisExpressionBacterialPromoterT7Available SinceOct. 7, 2014AvailabilityAcademic Institutions and Nonprofits only -
W118-1_hTGM2_C277S-Flag
Plasmid#180412Purposelentiviral transduction of human TGM2 transcript variant 2 with a point mutation (C277S) that inactivate the enzyme and also with a C-terminal Flag tagDepositorInsertTGM2_C277S (TGM2 Human)
UseLentiviralTagsFlagExpressionMammalianMutationC277SPromoterCMVAvailable SinceNov. 2, 2022AvailabilityAcademic Institutions and Nonprofits only -
pH6HTC_IGF2BP2a-Halo
Plasmid#175309PurposeProduction of HaloTag fusion proteinDepositorAvailable SinceOct. 8, 2021AvailabilityAcademic Institutions and Nonprofits only -
pET28-AviTag-DogTag-MBP
Plasmid#171773PurposeExpresses AviTag-DogTag-Maltose binding protein in bacterial cytoplasm. The AviTag enables site-specific biotinylation by BirA.DepositorInsertAviTag-DogTag-MBP
TagsHis6 and Thrombin cleavage siteExpressionBacterialMutationPeptide tag added to N-terminus of MBP via a GSGE…PromoterT7Available SinceJuly 29, 2021AvailabilityAcademic Institutions and Nonprofits only -
pHEA
Plasmid#168297PurposeBacterial surface display of polypeptides (nanobodies) in E. coliDepositorInsertEnterohemorraghic E. coli autotransporter EhaA C-terminal fragment (C-EhaA)
Tags6xHis, E-tag, and PelB signal peptideExpressionBacterialPromoterLacI-PlacAvailable SinceJune 9, 2021AvailabilityAcademic Institutions and Nonprofits only