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pICH85281

Plasmid

#50272

Depositor

Sylvestre Marillonnet, Nicola Patron

Article

Engler et al ACS Synth Biol. 2014 Nov 21;3(11):839-43

Insert

Promoter + 5'UTR, mas, (A. tumefaciens) + 5'UTR, omega (Tobacco Mosaic Virus)

Use

Plant expression

Mutation

BsaI/BbsI sites removed by point-mutation

Available Since

Feb. 18, 2014

Availability

Academic Institutions and Nonprofits only

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mg172

Plasmid

#34652

Depositor

Miriam Goodman

Article

Johnson et al Proc Natl Acad Sci U S A. 2011 Nov 2.

Insert

slo-1(A1;B1;C0) (slo-1 Nematode)

Use

Xenopus oocyte expression

Tags

Xenopus beta-globulin 3' UTR and Xenopus bet…

Available Since

Feb. 2, 2012

Availability

Academic Institutions and Nonprofits only

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pAGM5343

Plasmid

#50297

Depositor

Sylvestre Marillonnet, Nicola Patron

Article

Engler et al ACS Synth Biol. 2014 Nov 21;3(11):839-43

Insert

5'UTR, omega (Tobacco Mosaic Virus) + signal peptide, RAmy3A (O. sativa) + HIS tag

Use

Plant expression

Mutation

BsaI/BbsI sites removed by point-mutation

Available Since

Feb. 18, 2014

Availability

Academic Institutions and Nonprofits only

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pFP118

Plasmid

#21671

Depositor

James Haber

Article

Pâques et al Mol Cell Biol. 1997 Nov . 17(11):6765-71

Insert

Lacz (lacZ E. coli)

Use

Centromeric plasmid

Mutation

The second lacZ sequence has been modified, the E…

Available Since

Sept. 8, 2009

Availability

Academic Institutions and Nonprofits only
pLKO.1-puro-cfRabin8_5

Plasmid

#26714

Depositor

Keith Mostov

Article

Bryant et al Nat Cell Biol. 2010 Nov;12(11):1035-45.

Insert

dog Rabin8 RNAi

Use

Lentiviral and RNAi

Expression

Mammalian

Mutation

Dog Rabin8 alpha isoform RNAi

Available Since

Dec. 15, 2010

Availability

Academic Institutions and Nonprofits only
pCS2 xenopus twisted gastrulation

Plasmid

#24974

Depositor

Edward De Robertis

Article

Larrain et al Development. 2001 Nov . 128(22):4439-47.

Insert

twisted gastrulation (Twsg1 Mouse)

Tags

FLAG and chordin signal peptide

Expression

Mammalian

Available Since

June 18, 2010

Availability

Academic Institutions and Nonprofits only
pId1-B.IRES-YFP

Plasmid

#22668

Depositor

Robert Benezra

Article

Nam et al Cell Stem Cell. 2009 Nov 6. 5(5):515-26.

Insert

Inhibitor of DNA binding 1 (Id1 Mouse)

Tags

B.IRES-YFP

Expression

Mammalian

Available Since

Jan. 20, 2010

Availability

Academic Institutions and Nonprofits only

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pLKO.1-blast-cfRab25_2

Plasmid

#26711

Depositor

Keith Mostov

Article

Bryant et al Nat Cell Biol. 2010 Nov;12(11):1035-45.

Insert

dog Rab25 RNAi

Use

Lentiviral and RNAi

Expression

Mammalian

Mutation

RNAi to canine Rab25.

Available Since

Dec. 14, 2010

Availability

Academic Institutions and Nonprofits only

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3280 pcDNA3 Bid p22 cDNA

Plasmid

#8767

Depositor

Stanley Korsmeyer

Article

Wang et al Genes Dev 1996 Nov 15;10(22):2859-69.

Insert

Bid p22 ORF cDNA FL (Bid Mouse)

Expression

Mammalian

Available Since

July 6, 2005

Availability

Academic Institutions and Nonprofits only
pKlab676-HBEGF-2a-mWasabi-WPRE

Plasmid

#249029

Purpose

This plasmid expresses the mWasabi flourescent tag for integration into mammalian cell lines

Depositor

Georgios Karras

Article

Gracia et al Mol Cell. 2025 Nov 19:S1097-2765(25)0086

Insert

mWasabi

Use

CRISPR

Available Since

Jan. 21, 2026

Availability

Academic Institutions and Nonprofits only

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pKlab639-HBEGF-2a-mRuby3-WPRE

Plasmid

#249030

Purpose

This plasmid expresses the mRuby3 flourescent tag for integration into mammalian cell lines

Depositor

Georgios Karras

Article

Gracia et al Mol Cell. 2025 Nov 19:S1097-2765(25)0086

Insert

mRuby3

Use

CRISPR

Available Since

Jan. 21, 2026

Availability

Academic Institutions and Nonprofits only

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pKlab672-HBEGF-2a-mTagBFP2-WPRE

Plasmid

#249031

Purpose

This plasmid expresses the mTagBFP2 flourescent tag for integration into mammalian cell lines

Depositor

Georgios Karras

Article

Gracia et al Mol Cell. 2025 Nov 19:S1097-2765(25)0086

Insert

mTagBFP2

Use

CRISPR

Available Since

Jan. 21, 2026

Availability

Academic Institutions and Nonprofits only

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pKlab821-pcDNA3.1-HALO-3xFLAG

Plasmid

#249007

Purpose

This plasmid expresses the C terminally 3x-FLAG tagged HALO protein sequences for pulldown assays

Depositor

Georgios Karras

Article

Gracia et al Mol Cell. 2025 Nov 19:S1097-2765(25)0086

Insert

HALO

Tags

3x-FLAG-V5

Expression

Mammalian

Promoter

CMV Promoter

Available Since

Jan. 21, 2026

Availability

Academic Institutions and Nonprofits only

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pKlab965-PX459-BRCA1-Nterm-guide1

Plasmid

#249005

Purpose

This plasmid expresses the Cas9/gRNA that will target the HBEGF gene locus

Depositor

Georgios Karras

Article

Gracia et al Mol Cell. 2025 Nov 19:S1097-2765(25)0086

Insert

Cas9

Use

CRISPR

Tags

3xFLAG

Promoter

chicken beta-actin promoter

Available Since

Jan. 21, 2026

Availability

Academic Institutions and Nonprofits only

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pBA1580_ECOR31HamAB_CoPurification

Plasmid

#223371

Purpose

T7 protein expression of MBP-His-TEV-ECOR31 Hachiman

Depositor

Jennifer Doudna

Article

Tuck et al Cell. 2024 Nov 27;187(24):6914-6928.e20.

Insert

MBP-His-TEV-ECOR31 Hachiman

Tags

MBP-His-TEV

Expression

Bacterial

Available Since

Jan. 7, 2026

Availability

Academic Institutions and Nonprofits only

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pBA1464_pTet-ECOR31Ham_DELhamA_Mut

Plasmid

#223365

Purpose

Expression of ECOR31 Hachiman HamB (∆HamA) under the pTet promoter

Depositor

Jennifer Doudna

Article

Tuck et al Cell. 2024 Nov 27;187(24):6914-6928.e20.

Insert

ECOR31 Hachiman HamB (∆HamA)

Expression

Bacterial

Mutation

∆HamA

Available Since

Jan. 7, 2026

Availability

Academic Institutions and Nonprofits only

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pBA1465_pTet-ECOR31Ham_DELhamB_Mut

Plasmid

#223366

Purpose

Expression of ECOR31 Hachiman HamA (∆HamB) under the pTet promoter

Depositor

Jennifer Doudna

Article

Tuck et al Cell. 2024 Nov 27;187(24):6914-6928.e20.

Insert

ECOR31 Hachiman HamA (∆HamB)

Expression

Bacterial

Mutation

∆HamB

Available Since

Jan. 7, 2026

Availability

Academic Institutions and Nonprofits only

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pBA1467_pTet-ECOR31Ham_hamB_walkerB_Mut

Plasmid

#223367

Purpose

Expression of ECOR31 Hachiman mutant (HamB D431A) under the pTet promoter

Depositor

Jennifer Doudna

Article

Tuck et al Cell. 2024 Nov 27;187(24):6914-6928.e20.

Insert

ECOR31 Hachiman mutant (HamB D431A)

Expression

Bacterial

Mutation

HamB D431A

Available Since

Jan. 7, 2026

Availability

Academic Institutions and Nonprofits only

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pBA1444_ECOR31HamA_purification

Plasmid

#223369

Purpose

T7 protein expression of MBP-His-TEV-ECOR31 Hachiman HamA

Depositor

Jennifer Doudna

Article

Tuck et al Cell. 2024 Nov 27;187(24):6914-6928.e20.

Insert

MBP-His-TEV-ECOR31 Hachiman HamA

Tags

MBP-His-TEV

Expression

Bacterial

Available Since

Jan. 7, 2026

Availability

Academic Institutions and Nonprofits only

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pBA1445_ECOR31HamB_purification

Plasmid

#223370

Purpose

T7 protein expression of MBP-His-TEV-ECOR31 Hachiman HamB

Depositor

Jennifer Doudna

Article

Tuck et al Cell. 2024 Nov 27;187(24):6914-6928.e20.

Insert

MBP-His-TEV-ECOR31 Hachiman HamB

Tags

MBP-His-TEV

Expression

Bacterial

Available Since

Jan. 7, 2026

Availability

Academic Institutions and Nonprofits only

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