We narrowed to 10,321 results for: plasmids 101

1. pBEL1751

   Plasmid

   #139876

   Purpose

   Expression plasmid for naturally occurring 4xMCE protein (without TM region)

   Depositor

   Damian Ekiert, Gira Bhabha

   Article

   Isom et al Cell. 2020 Apr 30;181(3):653-664.e19. do

   Insert

   Desulfocapsa_sulfexigens_UWK_02604
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Desulfocapsa_sulfexigens_UWK_02604(38-533)-6xHis

   Promoter

   Available Since

   Aug. 26, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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2. PLKO.1-UPF3A-CDS

   Plasmid

   #136043

   Purpose

   UPF3A shRNA (Targeting CDS) inserted into the PLKO.1 plasmid (GCAGAAACCATTCTAAAGAAA)

   Depositor

   Anthony Leung

   Article

   Fischer et al Mol Cell. 2020 Apr 2;78(1):70-84.e6. doi

   Insert

   UPF3A (UPF3A Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Feb. 4, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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3. pCAS_gpyrG2

   Plasmid

   #90277

   Purpose

   (also pMST620-BB3_gpyrG2_cas9) CRISPR/Cas9 plasmid with gRNA for site pyrG2, Cas9

   Depositor

   Michael Sauer

   Article

   Sarkari et al Bioresour Technol. 2017 May 4. pii: S096

   Insert

   gRNA (pyrg2)
   

   Use

   A. niger

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available Since

   Dec. 12, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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4. pAP1071-2

   Plasmid

   #99502

   Purpose

   3XFlag::meGFP with 2 introns::Ollas::H2B::tbb-2 3utr::linker::TEV

   Depositor

   Geraldine Seydoux

   Article

   Paix et al Methods. 2017 May 15;121-122:86-93. doi:

   Insert

   3XFlag::meGFP with 2 introns::Ollas::H2B::tbb-2 3’utr::linker::TEV
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available Since

   Sept. 8, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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5. B108-Cre2

   Plasmid

   #73990

   Purpose

   B108 enhancer of Blimp1 gene drives IRES-Cre expression.

   Depositor

   Connie Cepko

   Article

   Wang et al Dev Cell. 2014 Sep 8;30(5):513-27. doi:

   Insert

   B108-IRES-Cre
   

   Use

   Tags

   Myc

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   May 22, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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6. Stagia3-Cre

   Plasmid

   #73991

   Purpose

   Cre in Stagia3 backbone; vector-Cre

   Depositor

   Connie Cepko

   Article

   Wang et al Dev Cell. 2014 Sep 8;30(5):513-27. doi:

   Insert

   Cre
   

   Use

   Tags

   Myc

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   May 19, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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7. TET1CD-CRY2-EGFP

   Plasmid

   #82555

   Purpose

   Encodes TET1 under LITE2.0 system

   Depositor

   Joseph Irudayaraj

   Article

   Lo et al Sci Rep. 2017 Feb 9;7:42047. doi: 10.103

   Inserts

   TET1 (TET1 Human)
   CRY2 (CRY2 Mustard Weed)
   EGFP
   

   Use

   AAV

   Tags

   2A, NLS(alpha-imp), and NLS-VP64

   Expression

   Mammalian

   Mutation

   CRY2PHR (N-terminal photolyase homology region do…

   Promoter

   EF-1alpha

   Available Since

   Sept. 6, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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8. pcDNA3.1+-Tornado-split-nLuc

   Plasmid

   #212612

   Purpose

   For the expression of a split nLuc that produces luminescence only when circularized. To test your IRES of interest, clone into the EcoRI, BsiWI sites.

   Depositor

   Samie Jaffrey

   Article

   Unti et al Cell Chem Biol. 2023 Oct 13:S2451-9456(2

   Insert

   Tornado-CVB3-split-nLuc
   

   Use

   Luciferase

   Tags

   Expression

   Mammalian

   Mutation

   C373T mutation in CVB3

   Promoter

   CMV

   Available Since

   Feb. 5, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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9. pBJ7

   Plasmid

   #167136

   Purpose

   A tetracyclin resistant plasmid for Tn7-mediated chromosomal integration of Photorhabdus luminescens luxCDABE operon with Pkan promoter

   Depositor

   Akira Mine

   Article

   Matsumoto et al Plant Commun. 2021 Jul 20;3(1):100227. d

   Insert

   luxCDABE
   

   Use

   Luciferase

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Kanamycin promoter

   Available Since

   June 9, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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10. pLV-eGFP

    Plasmid

    #36083

    Purpose

    3rd generation lentiviral transfer plasmid

    Depositor

    Pantelis Tsoulfas

    Article

    Enomoto et al Exp Neurol. 2013 Oct;248:170-82. doi: 10

    Has Service

    Cloning Grade DNA

    Type

    Empty backbone

    Use

    Lentiviral

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    June 1, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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11. pVP144

    Plasmid

    #222081

    Purpose

    Single component CRISPR-mediated base-editor for Agrobacterium genetic engineering visually marked with sfGFP for guide insertion and eforRED for plasmid eviction.

    Depositor

    Wayne Parrott

    Article

    Pennetti et al Biodes Res. 2025 March, 7:100001. doi: 1

    Insert

    dCas9-PmCDA1, sfGFP, sacB and eforRED
    

    Use

    CRISPR

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    Jan. 27, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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12. pNBU2_erm-TetR-P1T_DP-GH023 - NanoLuc

    Plasmid

    #117728

    Purpose

    Integration vector at an attB site in Bacteroides; contains 1kb cassette of aTC-regulatable promoter (RBS GH023) upstream of the NanoLuc gene

    Depositor

    Andrew Goodman

    Article

    Lim et al Cell. 2017 Apr 20;169(3):547-558.e15. do

    Insert

    NanoLuc
    

    Use

    Luciferase

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    Dec. 19, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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13. pVP143

    Plasmid

    #222080

    Purpose

    Single component CRISPR-mediated base-editor for Agrobacterium genetic engineering visually marked with sfGFP for guide insertion and AeBlue for plasmid eviction.

    Depositor

    Wayne Parrott

    Article

    Pennetti et al Biodes Res. 2025 March, 7:100001. doi: 1

    Insert

    dCas9-PmCDA1, sfGFP, sacB and AeBlue
    

    Use

    CRISPR

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    Jan. 27, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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14. pET29b-mVenus-dspB(E184Q)-6xHis

    Plasmid

    #176576

    Purpose

    Plasmid for overexpression of recombinant His-tagged fusion protein mVenus-DspB(E184Q), used as a fluorescent probe for detection of biofilm polysaccharide PNAG.

    Depositor

    Mark Nitz

    Article

    Eddenden et al Methods Enzymol. 2022;665:209-231. doi:

    Insert

    Dispersin B
    

    Use

    Tags

    Hexahistidine tag and mVenus

    Expression

    Bacterial

    Mutation

    E184Q

    Promoter

    T7

    Available Since

    Nov. 17, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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15. pREF-GFP kan

    Plasmid

    #122061

    Purpose

    reporter plasmid for termination and terminator override to express GFP. Marked with URA3

    Depositor

    Daniel Reines

    Article

    Reines et al Methods. 2019 Jan 4. pii: S1046-2023(18)

    Inserts

    intergenic terminator from yeast IMD2
    green fluorescent protein
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    GAL1 and GAL1 comes in pYES2

    Available Since

    Feb. 21, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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16. Syt1LeftArm-3XGS-GCaMP6s-SV40-3XP3-dsRed-SV40-Syt1RightArm

    Plasmid

    #159636

    Purpose

    Donor plasmid for generating Syt1:GCaMP6s strain in Aedes aegypti with CRISPR/Cas9

    Depositor

    Carolyn McBride

    Article

    Zhao et al Cell Rep Methods. 2021 Jul 26;1(3):10004

    Insert

    GCaMP6s
    

    Use

    Tags

    Expression

    Insect

    Mutation

    Promoter

    Available Since

    Sept. 15, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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17. pBN312

    Plasmid

    #86734

    Purpose

    GFP with Frt sites for FLP-mediated knock-out of gene function in C. elegans

    Depositor

    Peter Askjaer

    Article

    Munoz-Jimenez et al Genetics. 2017 Aug;206(4):1763-1778. doi

    Insert

    GFP_Frt_KO
    

    Use

    Tags

    Expression

    Worm

    Mutation

    Promoter

    Available Since

    April 3, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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18. PacrAB-acrAB-rfp + PmutS-mutS-yfp

    Plasmid

    #121443

    Purpose

    plasmid expressing translational fusions of AcrAB and MutS

    Depositor

    Mary Dunlop

    Article

    El Meouche et al Science. 2018 Nov 9;362(6415):686-690. d

    Inserts

    PmutS-mutS-yfp
    PacrAB-acrAB-rfp
    

    Use

    Synthetic Biology; Pbb vector

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    PacrAB and PmutS

    Available Since

    May 8, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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19. pCJH026_proD_Cas12c-multiplexing_CAM

    Plasmid

    #183073

    Purpose

    Plasmid expressing a Cas12c sgRNA targeting GFP and another sgRNA targeting RFP in one transcript for testing multiplexed repression

    Depositor

    Jennifer Doudna

    Article

    Huang et al Mol Cell. 2022 Jun 2;82(11):2148-2160.e4

    Insert

    Expressing two Cas12c sgRNAs (targeting GFP or RFP) in one transcript
    

    Use

    CRISPR

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    June 2, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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20. pET29b-AviTag-mEGFP-dspB(E184Q)-6xHis

    Plasmid

    #176573

    Purpose

    Plasmid for overexpression of recombinant AviTagged, His-tagged fusion protein mEGFP-DspB(E184Q), used as a fluorescent probe for detection of biofilm polysaccharide PNAG.

    Depositor

    Mark Nitz

    Article

    Eddenden et al Methods Enzymol. 2022;665:209-231. doi:

    Insert

    Dispersin B
    

    Use

    Tags

    Avitag, Hexahistidine tag, and mEGFP

    Expression

    Bacterial

    Mutation

    E184Q

    Promoter

    T7

    Available Since

    Nov. 17, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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