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plentiCRISPR-v2 Puro KIAA0141/DELE1_sg1

Plasmid

#218524

Purpose

sgRNA targeting human KIAA0141/DELE1

Depositor

Kivanc Birsoy

Article

Kenny et al Cell Metab. 2023 Jun 6;35(6):1057-1071.e

Insert

DELE1 (DELE1 Human)

Use

CRISPR and Lentiviral

Available Since

July 30, 2024

Availability

Academic Institutions and Nonprofits only

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plentiCRISPR-v2 Puro KIAA0141/DELE1_sg2

Plasmid

#218525

Purpose

sgRNA targeting human KIAA0141/DELE1

Depositor

Kivanc Birsoy

Article

Kenny et al Cell Metab. 2023 Jun 6;35(6):1057-1071.e

Insert

DELE1 (DELE1 Human)

Use

CRISPR and Lentiviral

Available Since

June 12, 2024

Availability

Academic Institutions and Nonprofits only

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pCALNL-mutKir2.1

Plasmid

#125579

Purpose

Cre-dependent expression of a non-conducting mutant of Kir2.1 (FLAG-tagged) in mammalian cells.

Depositor

Takeshi Imai

Article

Fujimoto et al Dev Cell. 2023 Jul 24;58(14):1221-1236.e

Insert

Kir2.1 (Kcnj2 Mouse)

Use

Cre/Lox

Tags

FLAG

Expression

Mammalian

Mutation

A non-conducting mutant (GYG -> AAA).

Available Since

July 8, 2019

Availability

Academic Institutions and Nonprofits only

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pESC-LEU-HsAIDSc

Plasmid

#60810

Purpose

to express human AID recoded for yeast codon usage

Depositor

Thomas Kunkel, Youri Pavlov

Article

Mayorov et al BMC Immunol. 2005 Jun 10;6:10.

Insert

AID (AICDA Human)

Tags

Myc

Expression

Yeast

Mutation

codon optimized for yeast

Promoter

GAL1

Available Since

Dec. 19, 2014

Availability

Academic Institutions and Nonprofits only
pLJM1-FH-AGO2-K525E

Plasmid

#91985

Purpose

Expresses FLAG-HA-AGO2 (K525E)

Depositor

Joshua Mendell

Article

Golden et al Nature. 2017 Feb 9;542(7640):197-202. do

Insert

AGO2 (AGO2 Human)

Use

Lentiviral

Tags

FLAG-HA

Expression

Mammalian

Mutation

K525E

Available Since

Aug. 25, 2017

Availability

Academic Institutions and Nonprofits only

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pGEM-HSPA5P

Plasmid

#44150

Purpose

a fragment containing 429 bp of the human HSPA5 promoter (-282 to +147)

Depositor

Joan Conaway

Article

Sela et al J Biol Chem. 2012 Jun 29;287(27):23035-4

Insert

HSPA5 promoter (HSPA5 Human)

Expression

Mammalian

Available Since

Feb. 4, 2014

Availability

Academic Institutions and Nonprofits only
pAPM-D4 miR30-AGO2 ts1

Plasmid

#115853

Purpose

AGO2 knockdown

Depositor

Jeremy Luban

Article

Yurkovetskiy et al Nat Microbiol. 2018 Dec;3(12):1354-1361.

Insert

AGO2 shRNA (AGO2 Human)

Use

Lentiviral

Promoter

SFFV

Available Since

Jan. 9, 2019

Availability

Academic Institutions and Nonprofits only

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pYFP-ErbB2ΔC776

Plasmid

#66947

Purpose

Mammalian expression of rat ErbB2 ΔC776 tagged with YFP

Depositor

Mads Lerdrup, Bo van_Deurs

Article

Lerdrup et al Mol Biol Cell. 2007 Sep;18(9):3656-66. E

Insert

ErbB2 (Erbb2 Rat)

Tags

6xHis, V5 Tag, and YFP

Expression

Mammalian

Mutation

Deletion of C776 (see depositor comment below on …

Promoter

CMV

Available Since

Sept. 15, 2015

Availability

Academic Institutions and Nonprofits only
GPR160-Tango

Plasmid

#66333

Purpose

Expression of G protein-coupled receptors for PRESTO-Tango: parallel receptorome expression and screening via transcriptional output, with transcriptional activation following arrestin translocation

Depositor

Bryan Roth

Article

Kroeze et al Nat Struct Mol Biol. 2015 May;22(5):362-

Insert

GPR160 (GPR160 Human)

Tags

FLAG

Expression

Mammalian

Promoter

CMV

Available Since

Sept. 15, 2015

Availability

Academic Institutions and Nonprofits only
pCIG AKT3 S472A

Plasmid

#73052

Purpose

Human AKT3 S472A

Depositor

Joseph Gleeson

Article

Baek et al Nat Med. 2015 Dec;21(12):1445-54. doi: 1

Insert

AKT3 S472A (AKT3 Human)

Expression

Mammalian

Mutation

S472A

Available Since

March 18, 2016

Availability

Academic Institutions and Nonprofits only

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RAT

Plasmid

#187416

Purpose

Expresses Na,K-ATPase (ATP1A1 and ATP1B1 in pFastBac Dual vector) in insect cells

Depositor

Susanne Dobler

Article

Mohammadi et al bioRxiv 2021.11.29.470343

Inserts

ATP1A1 (Atp1a1 Rat)
ATP1B1 (Atp1b1 Rat)

Expression

Insect

Promoter

PH and p10

Available Since

Oct. 5, 2022

Availability

Academic Institutions and Nonprofits only

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LUC45 (L/Sec/mutP)

Plasmid

#112148

Purpose

Mammalian expression vector to test the effect of substituting selenocysteine for Cys on luciferase enzyme activity

Depositor

Paul Copeland

Article

Mehta et al J Biol Chem. 2004 Sep 3;279(36):37852-9.

Insert

Luciferase coding region with Cysteine 258 mutated to selenocysteine (U) and MUTANT AUGA PHGPx SECIS core (Gpx4 Photinus pyralis, Rat)

Expression

Mammalian

Mutation

Cysteine 258 mutated to selenocysteine (U) and SE…

Available Since

July 6, 2018

Availability

Academic Institutions and Nonprofits only

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MBP-MEK1 S222TAG

Plasmid

#68302

Purpose

N-terminal MBP tagged human MEK1 gene S222TAG for bacterial expression

Depositor

Jesse Rinehart

Article

Pirman et al Nat Commun. 2015 Sep 9;6:8130. doi: 10.1

Insert

MBP-MEK1 S222TAG (MAP2K1 Synthetic, Human)

Tags

His and MBP

Expression

Bacterial

Available Since

Sept. 10, 2015

Availability

Academic Institutions and Nonprofits only

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pPICZ-mouseGIRK2-eGFP-StII-6xHis

Plasmid

#124277

Purpose

Expresses mouse GIRK2 as a TEV protease cleavable C-terminal fusion to eGFP, strep tag II, and 6x His tag

Depositor

Arthur Laganowsky

Article

Liu et al Nat Commun. 2019 Mar 22;10(1):1352. doi:

Insert

GIRK2 (Kcnj6 Mouse)

Tags

eGFP-StreptagII-6xHis

Expression

Yeast

Promoter

AOX1

Available Since

April 16, 2019

Availability

Academic Institutions and Nonprofits only

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pLenti-CamKIIa-monSTIM1

Plasmid

#161796

Purpose

Lentiviral vector expressing monSTIM1 in neurons.

Depositor

Won Do Heo

Article

Kim et al Nat Commun. 2020 Jan 10;11(1):210. doi:

Insert

EGFP-CRY2PHR(mon)-STIM1 (STIM1 Human)

Use

Lentiviral

Tags

EGFP

Promoter

CamKIIa

Available Since

Aug. 22, 2022

Availability

Academic Institutions and Nonprofits only

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pmx-MiGT

Plasmid

#172397

Purpose

Retroviral expression vector for murine direct cardiac reprogramming

Depositor

Li Qian

Article

Wang et al Cells. 2020 Jan 22;9(2). pii: cells90202

Insert

Mef2c-i, Gata4, Tbx5 (Gata4, Mef2c, Tbx5 Mouse)

Use

Retroviral

Available Since

July 28, 2021

Availability

Academic Institutions and Nonprofits only

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FusionRed-Caveolin-C-10

Plasmid

#56103

Purpose

Localization: Membrane, Excitation: 580, Emission: 608

Depositor

Michael Davidson

Article

Shemiakina et al Nat Commun. 2012;3:1204. doi: 10.1038/nc

Insert

Caveolin (CAV1 Human)

Tags

FusionRed

Expression

Mammalian

Promoter

CMV

Available Since

April 1, 2015

Availability

Industry, Academic Institutions, and Nonprofits
nes714tk/lacZ

Plasmid

#47614

Purpose

used to create transgenic mice expressing LacZ reporter with Nestin enhancer

Depositor

Urban Lendahl

Article

Lothian et al Eur J Neurosci. 1997 Mar;9(3):452-62.

Insert

nestin 2nd intron fragment (NES Human)

Use

Enhancer reporter

Tags

HSV tk promoter and lacZ

Mutation

714 bp from 3' end of 2nd intron

Promoter

HSV tk promoter

Available Since

Aug. 28, 2013

Availability

Academic Institutions and Nonprofits only
KCNE2_pcDNA6.2/EmGFP-Bsd

Plasmid

#176980

Purpose

Mammalian expression vector encoding KCNE2 and EmGFP-Bsd

Depositor

Roger Reeves

Article

Moyer et al Dis Model Mech. 2023 Apr 1;16(4):dmm0497

Insert

KCNE2 (KCNE2 Human)

Expression

Mammalian

Promoter

CMV

Available Since

April 19, 2022

Availability

Academic Institutions and Nonprofits only

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psiCHECK2-p27Kip1 3'UTR

Plasmid

#29476

Depositor

Judy Lieberman

Article

Lal et al Mol Cell. 2009 Sep 11. 35(5):610-25.

Insert

p27 Kip1 3'UTR (CDKN1B Human)

Expression

Mammalian

Available Since

April 27, 2011

Availability

Academic Institutions and Nonprofits only