We narrowed to 29,541 results for: REP

1. p.UTA.2.0 miR-27a-3p

   Plasmid

   #82438

   Purpose

   Dual Fluorescent endogenous miRNA sensor. Perfect complementary region of hsa-miR-27a-3p

   Depositor

   Jens Gruber

   Article

   Lemus-Diaz et al Sci Rep. 2017 Mar 24;7:45197. doi: 10.10

   Insert

   hsa-miR-27a-3p (MIR27A Human)
   

   Use

   Tags

   Expression

   Mutation

   Three copies hsa-miR-27a-3p Complementary region …

   Promoter

   Available since

   March 24, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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2. pNH-TrxT-G-S18

   Plasmid

   #127827

   Purpose

   Production of recombinant human ribosomal protein S18

   Depositor

   Ivanhoe Leung

   Article

   Correddu et al Sci Rep. 2019 Jun 20;9(1):8884. doi: 10.

   Insert

   RPS18 (RPS18 Human)
   

   Use

   Tags

   His6 - Thioredoxin - TEV

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Aug. 5, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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3. pAAV-FLEX-SaCas9-U6-sgGrin2a

   Plasmid

   #124850

   Purpose

   Mutagenesis of Grin2a

   Depositor

   Larry Zweifel

   Article

   Hunker et al Cell Rep. 2020 Mar 24;30(12):4303-4316.e

   Insert

   Grin2a (Grin2a Mouse)
   

   Use

   AAV, CRISPR, and Mouse Targeting

   Tags

   Expression

   Mutation

   Promoter

   Available since

   May 3, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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4. pAAV-FLEXfrt-SaCas9-U6-sgSlc17a6

   Plasmid

   #124860

   Purpose

   Mutagenesis of Slc17a6

   Depositor

   Larry Zweifel

   Article

   Hunker et al Cell Rep. 2020 Mar 24;30(12):4303-4316.e

   Insert

   Slc17a6 (Slc17a6 Mouse)
   

   Use

   AAV, CRISPR, and Mouse Targeting

   Tags

   Expression

   Mutation

   Promoter

   Available since

   May 6, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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5. pLenti GW V5 Eco/Dam mse EMD

   Plasmid

   #182675

   Purpose

   Lentiviral eukaryotic expression vector with E.Coli Dam methylation fused to mouse Emerin. Used in DamID experiments to methylate DNA that interacts in the proximity of Emerin.

   Depositor

   Bas van Steensel

   Article

   Amendola et al EMBO Rep. 2015 May;16(5):610-7. doi: 10.

   Insert

   Emerin (Emd Mouse)
   

   Use

   Lentiviral

   Tags

   E. Coli Dam and V5

   Expression

   Mammalian

   Mutation

   Promoter

   HSP

   Available since

   April 22, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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6. pAS_4xU6-PylT M15 (UUA) sfGFP 150TAA

   Plasmid

   #154775

   Purpose

   plasmid with 4xMma PylT (M15 mutant, UUA anticodon) cassette and ochre suppression reporter sfGFP 150 TAA stop, for transient transfection or stable, piggybac-mediated, integration

   Depositor

   Simon Elsaesser

   Article

   Meineke et al Cell Rep. 2020 Jun 23;31(12):107811. doi

   Insert

   sfGFP
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   150TAA in GFP reporter, UUA anticodon and G10A, …

   Promoter

   EF1

   Available since

   Oct. 1, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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7. pAAV-FLEXfrt-SaCas9-U6-sgGria2

   Plasmid

   #124868

   Purpose

   Mutagenesis of Gria2

   Depositor

   Larry Zweifel

   Article

   Hunker et al Cell Rep. 2020 Mar 24;30(12):4303-4316.e

   Insert

   Gria2 (Gria2 Mouse)
   

   Use

   AAV, CRISPR, and Mouse Targeting

   Tags

   Expression

   Mutation

   Promoter

   Available since

   May 3, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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8. pcDNA3-Lyso-AktAR2

   Plasmid

   #64933

   Purpose

   Lysosome-targeted FRET biosensor for monitoring Akt activity; targeted to cytosolic face of lysosomal membrane.

   Depositor

   Jin Zhang

   Article

   Zhou et al Cell Rep. 2015 Mar 11. pii: S2211-1247(1

   Insert

   LAMP1-AktAR2 (LAMP1 Budding Yeast, Synthetic, Human)
   

   Use

   Tags

   Cerulean3, Full-length LAMP1, and cpVenus[E172]

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   July 8, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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9. pLV_mU6-sgASCL1_hU6-sgRNA_hUbC-PuroR-P2A-GFP

   Plasmid

   #162337

   Purpose

   Lentiviral expression of sgASCL1 paired with a second S. pyogenes sgRNA with a GFP-P2A-PuroR selection marker

   Depositor

   Charles Gersbach

   Article

   Black et al Cell Rep. 2020 Dec 1;33(9):108460. doi:

   Insert

   S. pyogenes sgRNA
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   hU6; mU6

   Available since

   Feb. 11, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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10. pcDNA5/FRT/TO GABARAP(G116A)-mTagBFP2

    Plasmid

    #212112

    Purpose

    Expression of C-terminally tagged GABARAP(G116A) with mTagBFP2

    Depositor

    Dieter Willbold

    Article

    Uffing et al Autophagy Rep. 2024 May 16;3(1):2348899.

    Insert

    GABARAP(G116A)-mTagBFP2 (GABARAP Entacmaea quadricolor, Human)
    

    Use

    Tags

    mTagBFP2

    Expression

    Mammalian

    Mutation

    G116A (lipidation deficiency) of GABARAP

    Promoter

    CMV

    Available since

    May 22, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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11. pENTR-mCherry-attP(C31)-*BleoR

    Plasmid

    #183752

    Purpose

    phiC31 landing pad cassette.

    Depositor

    Richard Davis

    Article

    Blanch-Asensio et al Cell Rep Methods. 2022 Sep 22;2(10):1003

    Insert

    FRT-mCherry-attP-BleoR-F3
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Sept. 13, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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12. pCRE-iRFP670

    Plasmid

    #82696

    Purpose

    Expresses CRE and iRFP670 under two separate PGK promoters.

    Depositor

    Alan Mullen

    Article

    Daneshvar et al Cell Rep. 2016 Oct 4;17(2):353-365. doi:

    Insert

    CRE, iRFP670
    

    Use

    Cre/Lox

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Nov. 4, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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13. pKLV2-U6gRNA5(Ptpn23-g1)-PGKpuroBFP-W

    Plasmid

    #105034

    Purpose

    Lentiviral gRNA plasmid targeting mouse Ptpn23 , co-expression of TagBFP

    Depositor

    Kosuke Yusa

    Article

    Li et al Cell Rep. 2018 Jul 10;24(2):489-502. doi

    Insert

    Ptpn23 (Ptpn23 Mouse)
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    Sept. 4, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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14. pME18ST-d5-HT1B

    Plasmid

    #53108

    Purpose

    Expression of Drosophila 5-HT1B

    Depositor

    Leslie Vosshall

    Article

    Gasque et al Sci Rep. 2013;3:srep02120. doi: 10.1038/

    Insert

    5HT1B (5-HT1B Fly)
    

    Use

    Tags

    Expression

    Mutation

    Promoter

    Available since

    June 24, 2014

    Availability

    Academic Institutions and Nonprofits only

15. Gal4-TET1-878-911-WT(pL84)

    Plasmid

    #124400

    Purpose

    Gal4/VP16 luciferase reporter assay. Gal4 DBD fusion of WT TET1 SID.

    Depositor

    Shaun Cowley

    Article

    Chandru et al Sci Rep. 2018 Oct 2;8(1):14689. doi: 10.

    Insert

    Gal4-TET1-878-911 wildtype (TET1 Human)
    

    Use

    Tags

    gal4 dna binding domain

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    May 24, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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16. aCat TL2 (1-697 mTFP1-TSmod-Venus STOP)

    Plasmid

    #101298

    Purpose

    FRET-based tension sensor (TS) technology to develop a probe for molecular-level tension across alphaE-catenin.

    Depositor

    Alpha Yap

    Article

    Acharya et al Cell Rep. 2017 Mar 21;18(12):2854-2867.

    Insert

    alpha-Catenin
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Contains amino acids 1-698 of alpha-Catenin

    Promoter

    Available since

    June 5, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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17. pEN-X-hPGK-PuroR-Z

    Plasmid

    #218904

    Purpose

    golden gate cloning

    Depositor

    Sven Eyckerman

    Article

    Delhaye et al Cell Rep Methods. 2024 Jul 15;4(7):10081

    Insert

    hPGK-PuroR
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    246T>A and 398T>C

    Promoter

    Available since

    May 29, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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18. HES7-NLuc-2A-tdTomato

    Plasmid

    #130932

    Purpose

    Donor vector for tagging human HES7 C-terminus with NanoLuc and tdTomato

    Depositor

    James Thomson

    Article

    Chu et al Cell Rep. 2019 Aug 27;28(9):2247-2255.e5

    Insert

    HES7 (HES7 Human)
    

    Use

    Tags

    NanoLuc and tdTomato

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 11, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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19. pDEST40-2XFL-Src-KD

    Plasmid

    #140313

    Purpose

    Expresses Flag-tagged human Src-KD in mammalian cells for structure-function studies

    Depositor

    Mark Moasser

    Article

    Spassov et al Cell Rep. 2018 Oct 9;25(2):449-463.e4. d

    Insert

    Src mutant (SRC Human)
    

    Use

    Tags

    2X Flag

    Expression

    Mammalian

    Mutation

    Contains only the KD region

    Promoter

    CMV

    Available since

    April 28, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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20. pAS_4xhyb PylT A41AA C55A sfGFP 102TAG 150TAA

    Plasmid

    #154776

    Purpose

    plasmid with 4xhybrid PylT cassette (mutant A41AA C55A) and dual suppression reporter sfGFP 102TAG 150 TAA stop, for transient transfection or stable, piggybac-mediated, integration

    Depositor

    Simon Elsaesser

    Article

    Meineke et al Cell Rep. 2020 Jun 23;31(12):107811. doi

    Insert

    sfGFP
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    102TAG 150TAA in GFP reporter, hybrid PylT with A…

    Promoter

    EF1

    Available since

    Oct. 1, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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