We narrowed to 11,743 results for: pcDNA

1. pcDNA5-FRT-TO-Nprot-HF_GC3opt

   Plasmid

   #157732

   Purpose

   mammalian expression of C-terminally His8-Flag tagged SARS-CoV-2 N protein under control of a tetracycline-inducible promoter

   Depositor

   David Tollervey

   Article

   Bresson et al Wellcome Open Res. 2020 Nov 3;5:261. doi

   Insert

   Nprot-HF_GC3opt (N SARS-CoV-2)
   

   Use

   Tags

   His8-Flag

   Expression

   Mammalian

   Mutation

   codon optimized; gene expression was optimized by…

   Promoter

   Available since

   Aug. 10, 2020

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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2. pcDNA3.1 PhLP1-myc-6xHis

   Plasmid

   #218176

   Purpose

   Expresses phosducin-like protein 1 in mammalian cells

   Depositor

   Barry Willardson

   Article

   Wang et al Mol Cell. 2023 Nov 2;83(21):3852-3868.e6

   Insert

   Phosducin like protein 1 (PDCL Human)
   

   Use

   Tags

   6x His and Myc

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   April 26, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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3. pcDNA3-AKT-PH[R25C]-GFP

   Plasmid

   #18837

   Depositor

   Craig Montell

   Article

   Kwon et al Mol Cell. 2007 Feb 23. 25(4):491-503.

   Insert

   AKT-PH domain (AKT1 Human)
   

   Use

   Tags

   GFP

   Expression

   Mammalian

   Mutation

   R25C

   Promoter

   Available since

   July 14, 2008

   Availability

   Academic Institutions and Nonprofits only

   

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4. pCDNA4TO-3XFLAG Halo WT nsp1

   Plasmid

   #175412

   Purpose

   Expressing WT CoV2 nsp1 in mammalian cells. This contains a 3XFLAG Halo tag fusion to nsp1.

   Depositor

   Britt Glaunsinger

   Article

   Mendez et al Cell Rep. 2021 Oct 19;37(3):109841. doi:

   Insert

   CoV2 nsp1 (ORF1ab CoV2)
   

   Use

   Tags

   3XFLAG Halo

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Oct. 8, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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5. pcDNA3-DrFLINC-AKAR-Kras

   Plasmid

   #182334

   Purpose

   Genetically encoded fluorescent biosensor for super-resolution imaging of PKA activity; targeted to the plasma membrane. Compatible with multiplexed super-resolution imaging using a Dronpa-tagged POI.

   Depositor

   Jin Zhang

   Article

   Lin et al J Am Chem Soc. 2021 Sep 22;143(37):14951

   Insert

   DrFLINC-AKAR
   

   Use

   Tags

   6xHIS - T7 tag (gene 10 leader) - Xpress (TM) tag…

   Expression

   Mammalian

   Mutation

   Dronpa contains H193T mutation.

   Promoter

   CMV

   Available since

   June 2, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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6. pcDNA-LBR-EGFP-polyA

   Plasmid

   #59748

   Purpose

   Express LBR-EGFP in mammalian cells (CMV promoter). Also suitable for in vitro transcription (T7 promoter) for injecting mRNA into oocytes. This LBR contains N-terminal domain only.

   Depositor

   Yi Zhang

   Article

   Inoue et al Nat Struct Mol Biol. 2014 Jul;21(7):609-

   Insert

   LBR-EGFP
   

   Use

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   Promoter

   CMV and T7

   Available since

   Sept. 15, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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7. Human NCKX4-AS-long (pcDNA3.1+)

   Plasmid

   #75211

   Purpose

   Mammalian expression of SLC24A4

   Depositor

   Jonathan Lytton

   Article

   Li et al J Biol Chem. 2002 Dec 13;277(50):48410-7

   Insert

   SLC24A4 (SLC24A4 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   alternatively spliced exon present

   Promoter

   CMV

   Available since

   June 21, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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8. pcDNA3-Flag-Tet2 CD Mut

   Plasmid

   #72220

   Purpose

   Express flag-tagged mouse Tet2 catalytic domain - catalytic dead mutant.

   Depositor

   Yi Zhang

   Article

   Ito et al Science. 2011 Sep 2;333(6047):1300-3. do

   Insert

   Ten-Eleven Translocation 2 (Tet2 Mouse)
   

   Use

   Tags

   flag

   Expression

   Mammalian

   Mutation

   The plasmid expresses TET2 catalytic domain (cata…

   Promoter

   CMV

   Available since

   May 1, 2018

   Availability

   Academic Institutions and Nonprofits only

9. pcDNA3.1_Zeo_ARRB2-1-383-CTEV-2xHA

   Plasmid

   #194382

   Purpose

   Split TEV assays

   Depositor

   Michael Wehr

   Article

   Wu et al Biosensors (Basel). 2022 Dec 29;13(1):48

   Insert

   ARRB2-CTEV-2xHA (ARRB2 Synthetic, Human)
   

   Use

   Tags

   2xHA

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Feb. 8, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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10. pcDNA3 Flag C/EBPb LAP

    Plasmid

    #66979

    Purpose

    Expression plasmid for mouse C/EBPb LAP; adds Flag tag to the N-terminus of protein

    Depositor

    Christopher Vakoc

    Article

    Roe et al Mol Cell. 2015 May 12. pii: S1097-2765(1

    Insert

    C/EBP beta (Cebpb Mouse)
    

    Use

    Tags

    3xFlag

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    July 15, 2015

    Availability

    Academic Institutions and Nonprofits only

11. pcDNA3-Lyso-ExRai-AktAR2

    Plasmid

    #184052

    Purpose

    Lysosome-targeted ExRai-AktAR2.

    Depositor

    Jin Zhang

    Article

    Chen et al ACS Cent Sci. 2021 Dec 22;7(12):2009-202

    Insert

    Lyso-ExRai-AktAR2
    

    Use

    Tags

    Lysosome-associated membrane protein 1 (LAMP1).

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    June 6, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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12. pcDNA 3.1(-) mouse Egr2

    Plasmid

    #107997

    Purpose

    Expresses mouse Egr2 in mammalian cells

    Depositor

    Peter Johnson

    Article

    Salotti et al Mol Cell Biol. 2015 Mar;35(5):866-83. do

    Insert

    Egr2 (Egr2 Mouse)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    April 3, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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13. hE-cadherin/α-catenin fusion-pcDNA3

    Plasmid

    #45771

    Depositor

    Barry Gumbiner

    Article

    Gottardi et al J Cell Biol. 2001 May 28;153(5):1049-60.

    Insert

    hE-cadherin/Xe α-catenin fusion (CDH1, ctnna1 Frog, Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Human E-cadherin (aa 1-801) fused to Xenopus α-ca…

    Promoter

    CMV

    Available since

    June 14, 2013

    Availability

    Academic Institutions and Nonprofits only

14. pcDNA3-NES-YFP-CCTc

    Plasmid

    #184328

    Purpose

    Mammalian expression of the cytosolic localized YFP-CCTd(peptide of Cav1.2)

    Depositor

    Xiaodong Liu

    Article

    Yang et al Commun Biol. 2022 May 19;5(1):484. doi:

    Insert

    CCTc (CACNA1C Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    July 28, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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15. pcDNA4-Beclin1-(151-241)

    Plasmid

    #24393

    Depositor

    Qing Zhong

    Article

    Sun et al Proc Natl Acad Sci U S A. 2008 Dec 9. 10

    Insert

    Beclin1 (BECN1 Human)
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Amino acids 151-241 are deleted. Delta CC mutant.

    Promoter

    Available since

    April 30, 2010

    Availability

    Academic Institutions and Nonprofits only

16. Yellow Cameleon-Nano140/pcDNA3

    Plasmid

    #51966

    Purpose

    mammalian expression of Yellow Cameleon-Nano140, an ultrasensitive Ca2+ indicator with Kd = 140 nM

    Depositor

    Takeharu Nagai

    Article

    Horikawa et al Nat Methods. 2010 Sep;7(9):729-32. doi:

    Insert

    Yellow Cameleon-Nano140
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 18, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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17. pcDNA3.1-mas-KB-1753-Nluc

    Plasmid

    #158603

    Purpose

    Plasmid expressing KB-1753-Nluc in mammalian cells

    Depositor

    Mikel Garcia-Marcos

    Article

    Maziarz et al Cell. 2020 Jun 27. pii: S0092-8674(20)30

    Insert

    myristic acid attachment peptide (mas; MGSSKSKTSNS) - linker(GIKLGG) - KB1753 - linker (GGTGGS) - Nluc
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Sept. 30, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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18. pcDNA5/FRT/TO CBX1-APEX2

    Plasmid

    #129642

    Purpose

    Express heterochromatin-localized CBX1-APEX2 fusion in mammalian cells

    Depositor

    Nicholas Ingolia

    Article

    Padron et al Mol Cell. 2019 Aug 22;75(4):875-887.e5.

    Insert

    CBX1 (CBX1 Human)
    

    Use

    Tags

    1xFLAG and APEX2

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 26, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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19. pcdna6.2- myc ULK1 k46I

    Plasmid

    #27630

    Depositor

    Reuben Shaw

    Article

    Egan et al Science. 2010 Dec 23. ():.

    Insert

    mouse myc ULK1 K46I (Myc Mouse)
    

    Use

    Tags

    myc

    Expression

    Mammalian

    Mutation

    Initial M from ULK1 removed. Silent mutation at A…

    Promoter

    Available since

    Feb. 18, 2011

    Availability

    Academic Institutions and Nonprofits only

20. pcDNA5/FRT-Flag-Cul5

    Plasmid

    #31984

    Purpose

    Mammalian expression of Flag-tagged Cul5

    Depositor

    Joan Conaway

    Article

    Mahrour et al J Biol Chem. 2008 Mar 21;283(12):8005-13

    Insert

    Cul5 (CUL5 Human)
    

    Use

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 10, 2013

    Availability

    Academic Institutions and Nonprofits only