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TAL-L1-1.3-KRAB

Plasmid

#103034

Purpose

[Edit] Plasmid for IvT reaction with T3. Encodes LINE-1 specific TALE sequence followed by KRAB domain

Depositor

Maria-Elena Torres-Padilla

Article

Jachowicz et al Nat Genet. 2017 Aug 28. doi: 10.1038/ng.

Insert

TAL-L1-1.3-KRAB

Use

T3 in vitro transcription for expression in mamma…

Tags

HA and KRAB

Available Since

Nov. 30, 2017

Availability

Academic Institutions and Nonprofits only

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G1052C_BattB(GA)_EGFP_IRES-Spycatcher-sfmCherry3C(1-10)-P2A-shBleR

Plasmid

#200636

Purpose

Strong EGFP fluorescence recombination plasmid for Matreyek Bxb1(GA) landing pad in dual landing pad cells

Depositor

Kenneth Matreyek

Article

Roelle et al ACS Synth Biol. 2023 Nov 3. doi: 10.1021

Insert

EGFP

Expression

Mammalian

Available Since

Nov. 6, 2023

Availability

Academic Institutions and Nonprofits only

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pGAL4-DBD-MCS-long

Plasmid

#145246

Purpose

Backbone vector expressing GAL4 DNA-binding domain followed by MCS

Depositor

Denes Hnisz

Article

Basu et al Cell. 2020 May 28;181(5):1062-1079.e30.

Type

Empty backbone

Expression

Mammalian

Available Since

Sept. 21, 2020

Availability

Academic Institutions and Nonprofits only

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pETx-SR6c3

Plasmid

#176211

Purpose

Bacterial expression of the SR6c3 nanobody targeting the receptor binding domain of SARS-CoV-2 spike protein

Depositor

Aviv Regev

Article

Chen et al Nat Commun. 2021 Sep 17;12(1):5506. doi:

Insert

SR6c3

Tags

6XHis

Expression

Bacterial

Promoter

T7

Available Since

Sept. 30, 2021

Availability

Academic Institutions and Nonprofits only

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9xHRE-MinTK-CRE

Plasmid

#141146

Purpose

lentiviral expression vector to generate a hypoxia fate-mapping system

Depositor

Daniele Gilkes

Article

Godet et al Nat Commun. 2019 Oct 24;10(1):4862. doi:

Insert

9xHRE-MinTK-CRE

Use

Lentiviral

Available Since

Sept. 25, 2020

Availability

Academic Institutions and Nonprofits only

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pCAG-nDsRedFL-miR9/9*

Plasmid

#177805

Purpose

The pre-miR-9/9* sequence has been inserted into the EcoRV of pCAG-nDsRedFL-intron.

Depositor

Rikako Sanuki

Article

Suzuki et al Genes Cells. 2020 Nov;25(11):741-752. do

Insert

miR-9/9*

Expression

Mammalian

Available Since

Feb. 11, 2022

Availability

Academic Institutions and Nonprofits only

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pLKO.1P shTFRC

Plasmid

#169882

Purpose

Suppress TRFC

Depositor

Richard Possemato

Article

Terzi et al Sci Adv. 2021 May 26;7(22). pii: 7/22/ea

Insert

shRNA against TFRC

Use

Lentiviral and RNAi

Available Since

May 28, 2021

Availability

Academic Institutions and Nonprofits only

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pAAV-CRY2-CreN

Plasmid

#75268

Purpose

The second half of the photo-activatable cre recombinase

Depositor

Michael Bruchas

Article

Schindler et al Sci Rep. 2015 Sep 9;5:13627. doi: 10.103

Insert

second half of the cre recombinase fused to CRY2 (cryptochrome 2) that has absorbed blue light (Kennedy et al., 2010)

Use

AAV

Available Since

Feb. 17, 2017

Availability

Academic Institutions and Nonprofits only

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pAAV-CIBN-CreC

Plasmid

#75267

Purpose

The first half of the photo-activatable cre recombinase

Depositor

Michael Bruchas

Article

Schindler et al Sci Rep. 2015 Sep 9;5:13627. doi: 10.103

Insert

N-terminal portion of the CIB1 transcription factor

Use

AAV

Available Since

Oct. 25, 2016

Availability

Academic Institutions and Nonprofits only

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3149 pSFFV-neo Bcl-2 cDNA

Plasmid

#8750

Depositor

Stanley Korsmeyer

Article

Oltvai et al Cell 1993 Aug 27;74(4):609-19.

Insert

Bcl-2 (Bcl2 Mouse)

Expression

Mammalian

Available Since

April 4, 2006

Availability

Academic Institutions and Nonprofits only
GEARBOCS-SPARCL1-GeneTRAP

Plasmid

#218184

Purpose

To KO and genetrap mouse Hevin simultaneously

Depositor

Cagla Eroglu

Article

Bindu et al bioRxiv. 2023 Jan 19:2023.01.17.524433.

Insert

sgRNA (Sparcl1 Mouse)

Use

AAV and CRISPR

Available Since

Aug. 23, 2024

Availability

Academic Institutions and Nonprofits only

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GEARBOCS-SPARCL1-N-HATag

Plasmid

#218185

Purpose

To tag Hevin with HA at the N-terminal

Depositor

Cagla Eroglu

Article

Bindu et al bioRxiv. 2023 Jan 19:2023.01.17.524433.

Insert

sgRNA (Sparcl1 Mouse)

Use

AAV and CRISPR

Available Since

Aug. 23, 2024

Availability

Academic Institutions and Nonprofits only

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pWPXL-NP1

Plasmid

#72654

Purpose

Constitutive expression of NP1

Depositor

Ramon Trullas

Article

Figueiro-Silva et al J Neurosci. 2015 Apr 8;35(14):5504-21. d

Insert

NP1 (Nptx1 Rat)

Use

Lentiviral

Expression

Mammalian

Promoter

EF1a

Available Since

Jan. 15, 2016

Availability

Academic Institutions and Nonprofits only

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pMSCVpuro-T7-hAIM2-EGFP

Plasmid

#51608

Purpose

Express T7 and GFP-tagged hAIM2 in mammalian cells

Depositor

Emad Alnemri

Article

Fernandes-Alnemri et al Nature. 2009 Mar 26;458(7237):509-13. do

Insert

AIM2 (AIM2 Human)

Use

Retroviral

Tags

EGFP and T7

Expression

Mammalian

Promoter

MESV

Available Since

March 24, 2014

Availability

Academic Institutions and Nonprofits only

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pFLAG-ING2

Plasmid

#13295

Depositor

Curtis Harris

Article

Pedeux et al Mol Cell Biol. 2005 Aug . 25(15):6639-48

Insert

ING2 (INGX Human)

Tags

Flag

Expression

Mammalian

Available Since

Oct. 16, 2006

Availability

Academic Institutions and Nonprofits only
T7-RelA(S276E)

Plasmid

#24154

Depositor

Warner Greene

Article

Chen et al Mol Cell Biol. 2005 Sep . 25(18):7966-75

Insert

RelA (RELA Human)

Tags

T7 gp10

Expression

Mammalian

Mutation

S276E

Available Since

April 29, 2010

Availability

Academic Institutions and Nonprofits only
pIK127

Plasmid

#65631

Purpose

Expresses gfp::h2b in C. elegans cells from the ubiquitous eef-1A.1 (eft-3) promoter.

Depositor

Iskra Katic

Article

Katic et al G3 (Bethesda). 2015 Jun 3. pii: g3.115.0

Insert

Peft-3::gfp::h2b::tbb-2 3'UTR

Expression

Worm

Available Since

June 8, 2015

Availability

Academic Institutions and Nonprofits only

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miR-vector-RFP pCDNA

Plasmid

#97143

Purpose

for expression of miRNA and TagRFP

Depositor

Qing Deng

Article

Zhou et al Cell Rep. 2018 Feb 13;22(7):1810-1823. d

Type

Empty backbone

Tags

TagRFP

Expression

Mammalian

Available Since

July 26, 2018

Availability

Academic Institutions and Nonprofits only

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pRSCgfp-hAIM2

Plasmid

#51666

Purpose

Express GFP and hAIM2 in mammalian cells

Depositor

Emad Alnemri

Article

Fernandes-Alnemri et al Nature. 2009 Mar 26;458(7237):509-13. do

Inserts

AIM2 (AIM2 Human)
GFP

Expression

Mammalian

Promoter

CMV and RSV

Available Since

March 24, 2014

Availability

Academic Institutions and Nonprofits only

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pSI-check2-Tab1-3'UTR

Plasmid

#97155

Purpose

For dual luciferase report assay

Depositor

Qing Deng

Article

Zhou et al Cell Rep. 2018 Feb 13;22(7):1810-1823. d

Insert

Tab1 3'UTR

Expression

Mammalian

Available Since

July 26, 2018

Availability

Academic Institutions and Nonprofits only

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pET21a-mAIM2-His6

Plasmid

#51536

Purpose

Express His6-tagged mAIM2 in bacteria

Depositor

Emad Alnemri

Article

Fernandes-Alnemri et al Nature. 2009 Mar 26;458(7237):509-13. do

Insert

Aim2 (Aim2 Mouse)

Tags

His6 and T7

Expression

Bacterial

Promoter

lac operator

Available Since

March 20, 2014

Availability

Academic Institutions and Nonprofits only

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pSEVA221MBPc-AID-T7RNAP

Plasmid

#167975

Purpose

Expresses fusion protein MBPc-AID-T7RNAP in E. coli

Depositor

Luis Ángel Fernández

Article

Alvarez et al Nat Commun. 2020 Dec 22;11(1):6436. doi:

Insert

MBPc-AID-T7RNAP

Tags

MBPc and T7RNAP

Expression

Bacterial

Promoter

TetR-Ptet

Available Since

June 17, 2021

Availability

Academic Institutions and Nonprofits only

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SARS-CoV-2_N_deltaNTD

Plasmid

#157869

Purpose

express MBP tagged SARS-CoV-2 Nucleocapsid protein without the N terminal domain

Depositor

Nicolas Fawzi

Article

Perdikari et al EMBO J. 2020 Nov 17:e106478. doi: 10.152

Insert

N (N SARS-CoV-2)

Tags

MBP

Expression

Bacterial

Mutation

deltaNTD

Available Since

Nov. 23, 2020

Availability

Academic Institutions and Nonprofits only

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3922-48

Plasmid

#176497

Purpose

pTRE-EGFP-T2A-TFEB(wt)-polyA

Depositor

Albert La Spada

Article

Hsu et al Nat Commun. 2018 Mar 5;9(1):942. doi: 10

Insert

EGFP-T2A-TFEB(wild-type; full-length; human)

Expression

Mammalian

Promoter

TRE

Available Since

Nov. 18, 2021

Availability

Academic Institutions and Nonprofits only

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pcDNA5 FRT/TO Plk4 KD-mCherry

Plasmid

#80270

Purpose

mammalian expression plasmid for kinase dead Plk4

Depositor

Andrew Holland

Article

Moyer et al J Cell Biol. 2015 Jun 22;209(6):863-78.

Insert

Plk4 (PLK4 Human)

Tags

mCherry

Expression

Mammalian

Mutation

D154A kinase dead

Promoter

CMV

Available Since

Aug. 10, 2016

Availability

Academic Institutions and Nonprofits only

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pEBG-TFII-I-p70

Plasmid

#22187

Depositor

Ananda Roy

Article

Cheriyath et al Mol Cell Biol. 1998 Aug . 18(8):4444-54.

Insert

TFII-I (GTF2I Human)

Tags

GST and His

Expression

Mammalian

Mutation

Contains only amino acids 1-735 C-terminal residu…

Available Since

Nov. 13, 2009

Availability

Academic Institutions and Nonprofits only

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pLPC MYC hTel2

Plasmid

#22802

Depositor

Titia de Lange

Article

Takai et al Cell. 2007 Dec 28. 131(7):1248-59.

Insert

Telomere Maintenance 2 (TELO2 Human)

Use

Retroviral

Tags

MYC

Expression

Mammalian

Available Since

Jan. 22, 2010

Availability

Academic Institutions and Nonprofits only
pJYDN3p

Plasmid

#162457

Purpose

Positive control plasmid for pJYDN3n plasmid expression attempts. It contains both eUnaG2 bilirubin dependent fluorescent protein and ALFA-tag for DnbALFA based detection on the cell surface.

Depositor

Gideon Schreiber

Article

Zahradník et al bioRxiv Preprint 2020

Insert

Aga2p-eUnaG2 (AGA2 )

Use

Shuttle vector bacteria/yeast

Tags

ALFA tag, HA and myc tags, and eUnaG2 fluorescent…

Promoter

GAL1

Available Since

Feb. 19, 2021

Availability

Academic Institutions and Nonprofits only

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pCMV-Tag2B Flag BimS

Plasmid

#23283

Depositor

Roger Davis

Article

Hubner et al Mol Cell. 2008 May 23. 30(4):415-25.

Insert

pCMV Tag2b Bim S (Bcl2l11 Mouse)

Tags

Flag

Expression

Mammalian

Available Since

Feb. 26, 2010

Availability

Academic Institutions and Nonprofits only
Flag-nls-NgAgo-GK

Plasmid

#86435

Purpose

Expresses a Flag tag on the N-terminal

Depositor

Gaétan Burgio

Article

Khin et al PLoS One. 2017 Jun 13;12(6):e0178768. do

Insert

NgAgo

Tags

Flag tag

Expression

Mammalian

Promoter

CMV

Available Since

May 31, 2017

Availability

Academic Institutions and Nonprofits only

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