We narrowed to 16,473 results for: por

1. pSC-cUnaG-LPETG

   Plasmid

   #207635

   Purpose

   A plasmid encoding photocaged SpyCatcher (pSC) fused to C-terminal UnaG fragment and LPETG sortase recognition sequence.

   Depositor

   Cole DeForest

   Article

   Ruskowitz et al Nat Chem. 2023 May;15(5):694-704. doi: 1

   Insert

   photocaged SpyCatcher-GGSGGGSG-cUnaG-LPETG
   

   Use

   Tags

   6xHis

   Expression

   Bacterial

   Mutation

   Amber stop codon at SC's critical lysine

   Promoter

   T7

   Available since

   Oct. 5, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. pPV-C1-crRNA-cloning-EF1a-BA

   Plasmid

   #204623

   Purpose

   piggyBac vector for crRNA cloning and expression. Blasticidin selectable.

   Depositor

   Akitsu Hotta

   Article

   Kita et al Stem Cell Reports. 2023 Sep 12;18(9):175

   Type

   Empty backbone

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Sept. 27, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pFH2.17_GagZip-MCP-dpol-P2A-eGFP

   Plasmid

   #205532

   Purpose

   Express HIV GagZip-MCP-dpol-P2A-eGFP

   Depositor

   Michael Elowitz

   Article

   Horns et al Cell. 2023 Jul 10:S0092-8674(23)00689-X.

   Insert

   Gag-MCP-dpol
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Nucleocapsid replaced with leucine zipper, pol fr…

   Promoter

   CMV

   Available since

   Sept. 26, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pFH2.98_PB-EPN1-MCP_Myc-T2A-eGFP-PGK-Blast

   Plasmid

   #205543

   Purpose

   Express EPN1-MCP-Myc-T2A-eGFP and blasticidin resistance marker

   Depositor

   Michael Elowitz

   Article

   Horns et al Cell. 2023 Jul 10:S0092-8674(23)00689-X.

   Insert

   EPN1-MCP-Myc
   

   Use

   Piggybac

   Tags

   Expression

   Mammalian

   Mutation

   WT

   Promoter

   EF1

   Available since

   Sept. 19, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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5. CMV EGFP-PPM1H-H153D

   Plasmid

   #200432

   Purpose

   Mammalian expression of H153D mutation in PPM1H

   Depositor

   Erika Holzbaur

   Article

   Dou et al Cell Rep. 2023 May 1;42(5):112448. doi:

   Insert

   PPM1H protein phosphatase, Mg2+/Mn2+ dependent 1H
   

   Use

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   H153D

   Promoter

   CMV

   Available since

   May 22, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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6. pMC-6-AmCyan-P2A-NptII-9

   Plasmid

   #197742

   Purpose

   Phytobrick (MoClo) Level 0 Part

   Depositor

   Daniel Voytas

   Article

   Chamness et al Plant Genome. 2023 Mar 9:e20312. doi: 10

   Insert

   AmCyan-P2A-NptII bicistronic CDS
   

   Use

   Tags

   Expression

   Plant

   Mutation

   Promoter

   Available since

   May 11, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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7. 1K245-->R GLUT1-GFP

   Plasmid

   #200095

   Purpose

   1K245--> GLUT1 mutant with C-terminal GFP tag in pQCXIP vector

   Depositor

   Jason MacGurn

   Article

   Qualls-Histed et al iScience. 2023 Feb 6;26(3):106150. doi:

   Insert

   GLUT1 (SLC2A1 Human)
   

   Use

   Retroviral

   Tags

   GFP

   Expression

   Mammalian

   Mutation

   K245R

   Promoter

   CMV

   Available since

   May 9, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. TXNIP-py-FLAG

   Plasmid

   #200092

   Purpose

   TXNIP py motif mutant with C-terminal 3xFLAG tag in pQCXIP vector

   Depositor

   Jason MacGurn

   Article

   Qualls-Histed et al iScience. 2023 Feb 6;26(3):106150. doi:

   Insert

   TXNIP (TXNIP Human)
   

   Use

   Retroviral

   Tags

   3xFLAG

   Expression

   Mammalian

   Mutation

   py motifs mutated (aa331-334 and 375-378)

   Promoter

   CMV

   Available since

   May 3, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pBEL1954

   Plasmid

   #195689

   Purpose

   Expression/purification of 6xHis-TEV-MlaD(F118A/L123A)

   Depositor

   Damian Ekiert, Gira Bhabha

   Article

   MacRae et al J Biol Chem. 2023 Apr 24:104744. doi: 10

   Insert

   MlaD
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   6xHis-TEV-MlaD(F118A/L123A)

   Promoter

   Available since

   May 2, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pBEL1936

    Plasmid

    #195692

    Purpose

    Complementation of MlaF-MlaE-MlaDdelta153-183-MlaC-MlaB

    Depositor

    Damian Ekiert, Gira Bhabha

    Article

    MacRae et al J Biol Chem. 2023 Apr 24:104744. doi: 10

    Insert

    MlaFEDCB
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    MlaF-MlaE-MlaDdelta153-183-MlaC-MlaB

    Promoter

    Available since

    May 2, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pBEL1935

    Plasmid

    #195693

    Purpose

    Complementation of MlaF-MlaE-MlaDdelta173-183-MlaC-MlaB

    Depositor

    Damian Ekiert, Gira Bhabha

    Article

    MacRae et al J Biol Chem. 2023 Apr 24:104744. doi: 10

    Insert

    MlaFEDCB
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    MlaF-MlaE-MlaDdelta173-183-MlaC-MlaB

    Promoter

    Available since

    May 2, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pBEL1953

    Plasmid

    #195694

    Purpose

    Complementation of MlaF-MlaE-MlaD(F118A/L123A)-MlaC-MlaB

    Depositor

    Damian Ekiert, Gira Bhabha

    Article

    MacRae et al J Biol Chem. 2023 Apr 24:104744. doi: 10

    Insert

    MlaFEDCB
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    MlaF-MlaE-MlaD(F118A/L123A)-MlaC-MlaB

    Promoter

    Available since

    May 2, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pBEL1933

    Plasmid

    #195695

    Purpose

    Complementation of MlaF-MlaE-MlaD(F118A)-MlaC-MlaB

    Depositor

    Damian Ekiert, Gira Bhabha

    Article

    MacRae et al J Biol Chem. 2023 Apr 24:104744. doi: 10

    Insert

    MlaFEDCB
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    MlaF-MlaE-MlaD(F118A)-MlaC-MlaB

    Promoter

    Available since

    May 2, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pBEL1934

    Plasmid

    #195696

    Purpose

    Complementation of MlaF-MlaE-MlaD(L123A)-MlaC-MlaB

    Depositor

    Damian Ekiert, Gira Bhabha

    Article

    MacRae et al J Biol Chem. 2023 Apr 24:104744. doi: 10

    Insert

    MlaFEDCB
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    MlaF-MlaE-MlaD(L123A)-MlaC-MlaB

    Promoter

    Available since

    May 2, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pBEL2707

    Plasmid

    #195636

    Purpose

    Expression/purification of 6xHis-TEV-sfGFP-MlaA (227-251)

    Depositor

    Damian Ekiert, Gira Bhabha

    Article

    MacRae et al J Biol Chem. 2023 Apr 24:104744. doi: 10

    Insert

    sfGFP-MlaA
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    6xHis-TEV-sfGFP-MlaA (227-251)

    Promoter

    Available since

    May 2, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pBEL2753

    Plasmid

    #195637

    Purpose

    Expression/purification of 6xHis-TEV-sfGFP-MlaA (227-251; I241N)

    Depositor

    Damian Ekiert, Gira Bhabha

    Article

    MacRae et al J Biol Chem. 2023 Apr 24:104744. doi: 10

    Insert

    sfGFP-MlaA
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    6xHis-TEV-sfGFP-MlaA (227-251; I241N)

    Promoter

    Available since

    May 2, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pBEL2754

    Plasmid

    #195638

    Purpose

    Expression/purification of 6xHis-TEV-sfGFP-MlaA (227-251; L245N)

    Depositor

    Damian Ekiert, Gira Bhabha

    Article

    MacRae et al J Biol Chem. 2023 Apr 24:104744. doi: 10

    Insert

    sfGFP-MlaA
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    6xHis-TEV-sfGFP-MlaA (227-251; L245N)

    Promoter

    Available since

    May 2, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pBEL2755

    Plasmid

    #195639

    Purpose

    Expression/purification of 6xHis-TEV-sfGFP-MlaA (227-251; I248N)

    Depositor

    Damian Ekiert, Gira Bhabha

    Article

    MacRae et al J Biol Chem. 2023 Apr 24:104744. doi: 10

    Insert

    sfGFP-MlaA
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    6xHis-TEV-sfGFP-MlaA (227-251; I248N)

    Promoter

    Available since

    May 2, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pBEL2756

    Plasmid

    #195640

    Purpose

    Expression/purification of 6xHis-TEV-sfGFP-MlaA(227-251; delta238-251)

    Depositor

    Damian Ekiert, Gira Bhabha

    Article

    MacRae et al J Biol Chem. 2023 Apr 24:104744. doi: 10

    Insert

    sfGFP-MlaA
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    6xHis-TEV-sfGFP-MlaA(227-251; delta238-251)

    Promoter

    Available since

    May 2, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pBEL2757

    Plasmid

    #195641

    Purpose

    Expression/purification of 6xHis-TEV-sfGFP-MlaA(227-251; delta244-251)

    Depositor

    Damian Ekiert, Gira Bhabha

    Article

    MacRae et al J Biol Chem. 2023 Apr 24:104744. doi: 10

    Insert

    sfGFP-MlaA
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    6xHis-TEV-sfGFP-MlaA(227-251; delta244-251)

    Promoter

    Available since

    May 2, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pBEL2766

    Plasmid

    #196028

    Purpose

    Complementation of MlaF-MlaE-MlaDdelta141-183-MlaC-MlaB

    Depositor

    Damian Ekiert, Gira Bhabha

    Article

    MacRae et al J Biol Chem. 2023 Apr 24:104744. doi: 10

    Insert

    MlaFEDCB
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    MlaF-MlaE-MlaDdelta141-183-MlaC-MlaB

    Promoter

    Available since

    May 2, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pSLIK BirA* 3xFLAG-NUP37 zeo

    Plasmid

    #192333

    Purpose

    Lentiviral expression vector for an inducible 3xFLAG-BirA 3xFLAG-NUP37

    Depositor

    Kevin Janes

    Article

    Wang et al Nat Commun. 2023 Apr 13;14(1):2110. doi:

    Insert

    BirA*-3xFLAG-NUP37 (NUP37 Human)
    

    Use

    Lentiviral

    Tags

    3xFLAG

    Expression

    Mutation

    Promoter

    Available since

    April 28, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pEN_TT BirA*-3xFLAG-NUP37

    Plasmid

    #192311

    Purpose

    Gateway entry vector for an inducible 3xFLAG-BirA* 3xFLAG-NUP37

    Depositor

    Kevin Janes

    Article

    Wang et al Nat Commun. 2023 Apr 13;14(1):2110. doi:

    Insert

    BirA*-3xFLAG-NUP37 (NUP37 Human)
    

    Use

    Gateway entry vector

    Tags

    3xFLAG

    Expression

    Mutation

    Promoter

    Available since

    April 28, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pSEVA424::tsi5

    Plasmid

    #192960

    Purpose

    Expresses the protein Tsi51-76 for biological function studies in a broad-spectrum of organisms

    Depositor

    David Albesa-Jové

    Article

    Gonzalez-Magana et al Commun Biol. 2022 Nov 5;5(1):1189. doi:

    Insert

    tsi5
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Feb. 3, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pET29a(+)::D1141A

    Plasmid

    #192963

    Purpose

    Test the activity of the putative aspartyl protease motif DPXGL-(18)-DPXGL

    Depositor

    David Albesa-Jové

    Article

    Gonzalez-Magana et al Commun Biol. 2022 Nov 5;5(1):1189. doi:

    Insert

    Tse5_D1141A
    

    Use

    Tags

    9xhis + TEV tag

    Expression

    Bacterial

    Mutation

    D1141A

    Promoter

    Available since

    Dec. 30, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pET29a(+)::9xhis-Tse5

    Plasmid

    #192962

    Purpose

    Heterologous expression of Tse5 in E. coli Lemo21 cells for purification of Tse5-CT

    Depositor

    David Albesa-Jové

    Article

    Gonzalez-Magana et al Commun Biol. 2022 Nov 5;5(1):1189. doi:

    Insert

    Tse5
    

    Use

    Tags

    9xhis + TEV tag

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Dec. 30, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pBSKΔB-24xopto-TetO-no01

    Plasmid

    #174887

    Purpose

    Plasmid vector containing southern blot probe for opto-TetO in STREAMING-tag

    Depositor

    Hiroshi Ochiai

    Article

    Ohishi et al Nat Commun. 2022 Dec 20;13(1):7672. doi:

    Insert

    24xopto-TetO
    

    Use

    Southern blot probe

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Dec. 22, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. hsLEMD2-pX330

    Plasmid

    #179511

    Purpose

    Encodes gRNA for human LEMD2

    Depositor

    Yasushi Hiraoka

    Article

    Haraguchi et al Commun Biol. 2022 Jan 20;5(1):78. doi: 1

    Insert

    gRNA for LEMD2
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    Nov. 2, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. EK0312 pAAV-EF1a-DIO-EYFP-WPRE-HGHpA

    Plasmid

    #191148

    Purpose

    AAV expression of a Cre reporter with EYFP output

    Depositor

    Xiaojing Gao

    Article

    Kaseniit et al Nat Biotechnol. 2022 Oct 5. pii: 10.1038

    Insert

    DIO-EYFP
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    WT

    Promoter

    hEF1a

    Available since

    Oct. 26, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pNHT7

    Plasmid

    #186753

    Purpose

    A basic cloning vector for inserting genes with 5' and 3' UTRs for later subcloning via Golden Gate assembly.

    Depositor

    Bo Wang

    Article

    Hall et al Cell Rep Methods. 2022 Sep 20;2(10):1002

    Type

    Empty backbone

    Use

    Synthetic Biology; Cloning backbone

    Tags

    Expression

    Mutation

    Promoter

    T7

    Available since

    Oct. 24, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge