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EF1A_Hygro_P2A_RUNX1_V63E_Barcode

Plasmid

#170926

Purpose

Overexpression of RUNX1 V63E mutant protein.

Depositor

Prashant Mali

Article

Ozturk et al Cell Rep. 2024 Jul 4;43(7):114436. doi:

Insert

RUNX1 (RUNX1 Human)

Use

Lentiviral

Expression

Mammalian

Mutation

V63E

Promoter

Ef1A

Available Since

July 22, 2024

Availability

Academic Institutions and Nonprofits only

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EF1A_Hygro_P2A_RUNX1_R64P_Barcode

Plasmid

#170927

Purpose

Overexpression of RUNX1 R64P mutant protein.

Depositor

Prashant Mali

Article

Ozturk et al Cell Rep. 2024 Jul 4;43(7):114436. doi:

Insert

RUNX1 (RUNX1 Human)

Use

Lentiviral

Expression

Mammalian

Mutation

R64P

Promoter

Ef1A

Available Since

July 22, 2024

Availability

Academic Institutions and Nonprofits only

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EF1A_Hygro_P2A_RUNX1_T65I_Barcode

Plasmid

#170928

Purpose

Overexpression of RUNX1 T65I mutant protein.

Depositor

Prashant Mali

Article

Ozturk et al Cell Rep. 2024 Jul 4;43(7):114436. doi:

Insert

RUNX1 (RUNX1 Human)

Use

Lentiviral

Expression

Mammalian

Mutation

T65I

Promoter

Ef1A

Available Since

July 22, 2024

Availability

Academic Institutions and Nonprofits only

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pENTR-TMEM144

Plasmid

#221426

Purpose

Gateway entry clone with codon-optimized ORF sequence for subcloning into custom expression vectors

Depositor

RESOLUTE Consortium, Giulio Superti-Furga

Article

Superti-Furga et al Nat Rev Drug Discov. 2020 Jul;19(7):429-

Insert

TMEM144 (TMEM144 Human)

Expression

Mammalian

Available Since

July 18, 2024

Availability

Academic Institutions and Nonprofits only

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pENTR-Sensor_P2A_SLC2A4

Plasmid

#221429

Purpose

Gateway entry clone encoding a polyprotein that is cleaved to FRET glucose sensor and SLC2A4 proteins

Depositor

RESOLUTE Consortium, Giulio Superti-Furga

Article

Superti-Furga et al Nat Rev Drug Discov. 2020 Jul;19(7):429-

Insert

Sensor_P2A_SLC2A4 (SLC2A4 Synthetic)

Expression

Mammalian

Available Since

July 18, 2024

Availability

Academic Institutions and Nonprofits only

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pMXs-3xFlag-SPNS1

Plasmid

#198568

Purpose

SPNS1 codon-optimized orf with n-terminal 3xFlag in pMXs-IRES-Blast vector

Depositor

Monther Abu-Remaileh

Article

Scharenberg et al Sci Adv. 2023 Apr 21;9(16):eadf8966. doi

Insert

SPNS1 with n-terminal 3xFlag (SPNS1 Human)

Use

Retroviral

Tags

3xFlag

Expression

Mammalian

Available Since

July 12, 2024

Availability

Academic Institutions and Nonprofits only

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SSH-pSUMO-β′-COPI-WD40-Tyr33Ala

Plasmid

#208170

Purpose

Bacterial expression plasmid for Saccharomyces cerevisiae β′-COPI-WD40-Tyr33Ala mutant (residues 1-301)

Depositor

Syed Saif Hasan

Article

Dey et al Nat Commun. 2023 Dec 15;14(1):8358. doi:

Insert

N-terminal domain of yeast β′-COPI-WD40 subunit (1-301) with Tyr33Ala substitution

Tags

His-tag, SUMO-tag, and Strep-tag

Expression

Bacterial

Available Since

June 21, 2024

Availability

Academic Institutions and Nonprofits only

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SSH-pcDNA3.1-SSH-SARS-CoV-2-S-Thr1273Glu

Plasmid

#218461

Purpose

Mammalian expression plasmid for SARS-CoV-2 spike (1273 residues; Thr1273Glu substitution)

Depositor

Syed Saif Hasan

Article

Dey et al Nat Commun. 2023 Dec 15;14(1):8358. doi:

Insert

SARS-CoV-2 spike protein (1273 residues) carrying Thr1273Glu substitution (S )

Expression

Mammalian

Mutation

Thr1273AGlu substitution

Available Since

June 21, 2024

Availability

Academic Institutions and Nonprofits only

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SSH-pcDNA3.1-SSH-SARS-CoV-2-S-Thr1273Asp

Plasmid

#218462

Purpose

Mammalian expression plasmid for SARS-CoV-2 spike (1273 residues; Thr1273Asp substitution)

Depositor

Syed Saif Hasan

Article

Dey et al Nat Commun. 2023 Dec 15;14(1):8358. doi:

Insert

SARS-CoV-2 spike protein (1273 residues) carrying Thr1273Asp substitution (S )

Expression

Mammalian

Mutation

Thr1273Asp substitution

Available Since

June 21, 2024

Availability

Academic Institutions and Nonprofits only

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H2B-GFP-INCENP∆cen

Plasmid

#184044

Purpose

Expresses H2B-GFP fused to INCENP∆cen

Depositor

Lienhard Schmitz

Article

Pfisterer et al Open Biol. 2024 May;14(5):230460. doi: 1

Insert

INCENP (INCENP Human)

Tags

GFP and H2B

Expression

Mammalian

Mutation

Truncated to 1-862

Promoter

CMV

Available Since

June 10, 2024

Availability

Academic Institutions and Nonprofits only

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ENTR_BN001T-N_START

Plasmid

#218058

Purpose

RESOLUTE SLC binder cloning plasmid

Depositor

RESOLUTE Consortium, Giulio Superti-Furga

Article

Gelová et al J Mol Biol. 2024 Jun 13:168665. doi: 10.

Insert

BN001T-N_START

Use

Cloning plasmid

Available Since

May 17, 2024

Availability

Academic Institutions and Nonprofits only

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ENTR_BN006A-P_START

Plasmid

#218059

Purpose

RESOLUTE SLC binder cloning plasmid

Depositor

RESOLUTE Consortium, Giulio Superti-Furga

Article

Gelová et al J Mol Biol. 2024 Jun 13:168665. doi: 10.

Insert

BN006A-P_START

Use

Cloning plasmid

Available Since

May 17, 2024

Availability

Academic Institutions and Nonprofits only

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ENTR_BN001M-T_START

Plasmid

#218060

Purpose

RESOLUTE SLC binder cloning plasmid

Depositor

RESOLUTE Consortium, Giulio Superti-Furga

Article

Gelová et al J Mol Biol. 2024 Jun 13:168665. doi: 10.

Insert

BN001M-T_START

Use

Cloning plasmid

Available Since

May 17, 2024

Availability

Academic Institutions and Nonprofits only

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plentiCRISPR-v2 Puro FLVCR1_sg5

Plasmid

#218522

Purpose

sgRNA targeting human FLVCR1

Depositor

Kivanc Birsoy

Article

Kenny et al Cell Metab. 2023 Jun 6;35(6):1057-1071.e

Insert

FLVCR1 (FLVCR1 Human)

Use

CRISPR and Lentiviral

Available Since

May 7, 2024

Availability

Academic Institutions and Nonprofits only

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plentiCRISPR-v2 Puro CHKA_sg2

Plasmid

#218523

Purpose

sgRNA targeting human CHKA

Depositor

Kivanc Birsoy

Article

Kenny et al Cell Metab. 2023 Jun 6;35(6):1057-1071.e

Insert

CHKA (CHKA Human)

Use

CRISPR and Lentiviral

Available Since

May 6, 2024

Availability

Academic Institutions and Nonprofits only

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plentiCRISPR-v1 GFP FLVCR1_sg5

Plasmid

#218521

Purpose

sgRNA targeting human FLVCR1

Depositor

Kivanc Birsoy

Article

Kenny et al Cell Metab. 2023 Jun 6;35(6):1057-1071.e

Insert

FLVCR1 (FLVCR1 Human)

Use

CRISPR and Lentiviral

Available Since

May 6, 2024

Availability

Academic Institutions and Nonprofits only

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B2M-SDMutation-gRNA

Plasmid

#215547

Purpose

gRNA targeting B2M to introduce splice donor mutation on the first intron of the locus

Depositor

Kai Wang

Article

Zhao et al STAR Protoc. 2024 May 23;5(2):103084. do

Insert

B2M (B2M Human)

Use

CRISPR

Available Since

March 14, 2024

Availability

Academic Institutions and Nonprofits only

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EF1a-SRRD-V5-APEX2 (in pLEX_307)

Plasmid

#214921

Purpose

constitutive expression of SRRD fused to V5 and APEX2 - used for APEX2 proximity biotinylation

Depositor

Ophir Shalem

Article

Sweeney et al PLoS Genet. 2024 Feb 5;20(2):e1011138. d

Insert

SRRD (SRRD Human)

Use

Lentiviral

Tags

V5-APEX2

Available Since

March 11, 2024

Availability

Academic Institutions and Nonprofits only

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pET SUMO-Doc2β C2AB (G361C)

Plasmid

#213631

Purpose

Bacterial expression of SUMO-tagged rat Doc2β C2AB (G361C)

Depositor

Edwin Chapman

Article

Courtney et al Nat Commun. 2023 Nov 27;14(1):7761. doi:

Insert

Doc2β C2AB (G361C) (Doc2b Rat)

Tags

SUMO

Expression

Bacterial

Mutation

G361C, all original cysteines mutated to alanines

Available Since

March 6, 2024

Availability

Academic Institutions and Nonprofits only

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INS-3'gRNA

Plasmid

#210468

Purpose

gRNA targeting INS 3' terminal for CRISPR-Cas9-mediated knock-in

Depositor

Kai Wang

Article

Zhao et al STAR Protoc. 2024 May 23;5(2):103084. do

Insert

INS (INS Human)

Use

CRISPR

Available Since

Jan. 11, 2024

Availability

Academic Institutions and Nonprofits only

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pLKO.3R/MPL-shRNA4

Plasmid

#204529

Purpose

Lentiviral expression of MPL-shRNA4; gene knock down of human MPL

Depositor

Norio Komatsu

Article

Araki et al Blood. 2016 Jan 27. pii: blood-2015-09-6

Insert

MPL-shRNA4 (MPL Human)

Use

Lentiviral

Available Since

Sept. 12, 2023

Availability

Academic Institutions and Nonprofits only

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pLKO.3R/MPL-shRNA3

Plasmid

#204528

Purpose

Lentiviral expression of MPL-shRNA3; gene knock down of human MPL

Depositor

Norio Komatsu

Article

Araki et al Blood. 2016 Jan 27. pii: blood-2015-09-6

Insert

MPL-shRNA3 (MPL Human)

Use

Lentiviral

Available Since

Sept. 12, 2023

Availability

Academic Institutions and Nonprofits only

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pLKO.3R/MPL-shRNA2

Plasmid

#204527

Purpose

Lentiviral expression of MPL-shRNA2; gene knock down of human MPL

Depositor

Norio Komatsu

Article

Araki et al Blood. 2016 Jan 27. pii: blood-2015-09-6

Insert

MPL-shRNA2 (MPL Human)

Use

Lentiviral

Available Since

Sept. 12, 2023

Availability

Academic Institutions and Nonprofits only

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pLKO.3R/MPL-shRNA1

Plasmid

#204526

Purpose

Lentiviral expression of MPL-shRNA1; gene knock down of human MPL

Depositor

Norio Komatsu

Article

Araki et al Blood. 2016 Jan 27. pii: blood-2015-09-6

Insert

MPL-shRNA1 (MPL Human)

Use

Lentiviral

Available Since

Sept. 12, 2023

Availability

Academic Institutions and Nonprofits only

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pX330_SAFBgRNA9

Plasmid

#196108

Purpose

Contains guide RNA to 3' end of mouse SAFB gene for safb1/2 dko. Used with Addgene IDs: 196103, 196106, 196107

Depositor

Mauro Calabrese

Article

Cherney et al RNA. 2023 Jul 19:rna.079569.122. doi: 10

Insert

SAFB (Safb Mouse)

Use

CRISPR and Mouse Targeting

Expression

Bacterial and Mammalian

Available Since

Sept. 5, 2023

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-INSRR-V5/HIS

Plasmid

#201981

Purpose

expression of human INSRR receptor tyrosine kinase in mammalian cells

Depositor

Pavel Krejčí

Article

Gudernova et al Elife. 2017 Feb 15;6. pii: e21536. doi:

Insert

INSRR receptor tyrosine kinase (INSRR Human)

Tags

V5, 6xHis

Expression

Mammalian

Promoter

CMV

Available Since

June 21, 2023

Availability

Academic Institutions and Nonprofits only

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pRetroX-TRE3G_NT-GSDMD_I105N_NoCys

Plasmid

#201177

Purpose

Dox-inducible expression of NT-GSDMD gene in mammalian cells by retroviral transduction

Depositor

Jonathan Kagan

Article

Devant et al Cell Rep. 2023 Jan 31;42(1):112008. doi:

Insert

Gasdermin D N-terminal domain (Gsdmd Mouse)

Use

Retroviral

Mutation

aa1-276 only, I105N, C39A, C57A, C77A, C122A, C2…

Promoter

TRE3G

Available Since

May 26, 2023

Availability

Academic Institutions and Nonprofits only

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pRetroX-TRE3G_NT-GSDMD_I105N_C192only

Plasmid

#201178

Purpose

Dox-inducible expression of NT-GSDMD gene in mammalian cells by retroviral transduction

Depositor

Jonathan Kagan

Article

Devant et al Cell Rep. 2023 Jan 31;42(1):112008. doi:

Insert

Gasdermin D N-terminal domain (Gsdmd Mouse)

Use

Retroviral

Mutation

aa1-276 only, I105N, C39A, C57A, C77A, C122A, C2…

Promoter

TRE3G

Available Since

May 26, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pRetroX-TRE3G_NT-GSDMD_NoCys

Plasmid

#201173

Purpose

Dox-inducible expression of NT-GSDMD gene in mammalian cells by retroviral transduction

Depositor

Jonathan Kagan

Article

Devant et al Cell Rep. 2023 Jan 31;42(1):112008. doi:

Insert

Gasdermin D N-terminal domain (Gsdmd Mouse)

Use

Retroviral

Mutation

aa1-276 only, C39A, C57A, C77A, C122A, C265A, C19…

Promoter

TRE3G

Available Since

May 26, 2023

Availability

Academic Institutions and Nonprofits only

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pSLIK mCh-V5-NES-EGFP-EGFR NLS zeo

Plasmid

#192337

Purpose

Lentiviral expression vector for an inducible mCh-V5-NES-EGFP-EGFR NLS

Depositor

Kevin Janes

Article

Wang et al Nat Commun. 2023 Apr 13;14(1):2110. doi:

Insert

mCh-V5-NES-EGFP-EGFR NLS (EGFR Human)

Use

Lentiviral

Tags

3xFLAG

Available Since

April 28, 2023

Availability

Academic Institutions and Nonprofits only

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pSLIK mCh-V5-NES-EGFP-Erbb2 NLS zeo

Plasmid

#192339

Purpose

Lentiviral expression vector for an inducible mCh-V5-NES-EGFP-Erbb2 NLS

Depositor

Kevin Janes

Article

Wang et al Nat Commun. 2023 Apr 13;14(1):2110. doi:

Insert

mCh-V5-NES-EGFP-Erbb2 NLS (Erbb2 Rat)

Use

Lentiviral

Tags

3xFLAG

Available Since

April 28, 2023

Availability

Academic Institutions and Nonprofits only

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pEN_TT mCh-V5-NES-EGFP-EGFR NLS

Plasmid

#192315

Purpose

Gateway entry vector for an inducible mCh-V5-NES-EGFP-EGFR NLS

Depositor

Kevin Janes

Article

Wang et al Nat Commun. 2023 Apr 13;14(1):2110. doi:

Insert

mCh-V5-NES-EGFP-EGFR NLS (EGFR Human)

Use

Gateway Cloning

Tags

3xFLAG

Available Since

April 28, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pBabe NGF-ERBB2-2xFKBP-HA hygro

Plasmid

#192292

Purpose

Retroviral expression vector for NGF-ERBB2 -2xFKBP-HA construct

Depositor

Kevin Janes

Article

Wang et al Nat Commun. 2023 Apr 13;14(1):2110. doi:

Insert

NGF-ERBB2-2xFKBP-HA (ERBB2 Human)

Use

Retroviral

Tags

HA

Available Since

April 28, 2023

Availability

Academic Institutions and Nonprofits only

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pROS_phleo-Tps1/Gsy2

Plasmid

#196612

Purpose

Encoding guide RNAs for the knock out of TPS1 and GSY2 genes

Depositor

Matthias Heinemann

Article

Takhaveev et al Nat Metab. 2023 Feb;5(2):294-313. doi: 1

Insert

TPS1, GSY2 (TPS1, GSY2 Budding Yeast)

Expression

Yeast

Promoter

SNR52

Available Since

March 23, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pROS13-Tps2/Gsy1

Plasmid

#196613

Purpose

Encoding guide RNAs for the knock out of TPS2 and GSY1 genes

Depositor

Matthias Heinemann

Article

Takhaveev et al Nat Metab. 2023 Feb;5(2):294-313. doi: 1

Insert

S000001911 (TPS2, GSY1 Budding Yeast)

Expression

Yeast

Promoter

SNR52

Available Since

March 15, 2023

Availability

Academic Institutions and Nonprofits only

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GA46

Plasmid

#196614

Purpose

Plasmid used for the C-terminal tagging of Atp3 with mCherry and the auxin-inducible degron in yeast

Depositor

Matthias Heinemann

Article

Takhaveev et al Nat Metab. 2023 Feb;5(2):294-313. doi: 1

Insert

ATP3::mCherry-AID(71-114)-NatMX (ATP3 Budding Yeast)

Tags

AID(71-114) and mCherry

Expression

Yeast

Available Since

March 1, 2023

Availability

Academic Institutions and Nonprofits only

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pUGP1.1

Plasmid

#196615

Purpose

Plasmid used for the C-terminal tagging of Ugp1 with mCherry and the auxin-inducible degron in yeast

Depositor

Matthias Heinemann

Article

Takhaveev et al Nat Metab. 2023 Feb;5(2):294-313. doi: 1

Insert

UGP1::mCherry-AID(71-114)-NatMX (UGP1 Budding Yeast)

Tags

AID(71-114) and mCherry

Expression

Yeast

Promoter

Native promoter

Available Since

March 1, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pKTop::sptse5-CT (1169-1229)

Plasmid

#192948

Purpose

For membrane insertion study; expresses Tse5-CT (1169-1229)/PhoA (22-472)/LacZ (4-60)fusion with PelB signalpeptide fused at the N-terminus

Depositor

David Albesa-Jové

Article

Gonzalez-Magana et al Commun Biol. 2022 Nov 5;5(1):1189. doi:

Insert

sptse5-CT (1169-1229)

Tags

PelB signal peptide

Expression

Bacterial

Mutation

encodes for tse5 (1169-1229)

Available Since

Dec. 30, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pKTop::tse5-CT (1169-1300)

Plasmid

#192956

Purpose

For membrane insertion study; Expresses tse5-CT (1169-1300) /PhoA (22-472)/LacZ (4-60)fusion with PelB signalpeptide fused at the N-terminus

Depositor

David Albesa-Jové

Article

Gonzalez-Magana et al Commun Biol. 2022 Nov 5;5(1):1189. doi:

Insert

tse5-CT (1169-1300)

Expression

Bacterial

Mutation

encodes for tse5 (1169-1300)

Available Since

Dec. 30, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pKTop::tse5-CT (1169-1317)

Plasmid

#192957

Purpose

For membrane insertion study; Expresses tse5-CT (1169-1317) /PhoA (22-472)/LacZ (4-60)fusion with PelB signalpeptide fused at the N-terminus

Depositor

David Albesa-Jové

Article

Gonzalez-Magana et al Commun Biol. 2022 Nov 5;5(1):1189. doi:

Insert

tse5-CT (1169-1317)

Expression

Bacterial

Mutation

encodes for tse5 (1169-1317)

Available Since

Dec. 30, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge

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