We narrowed to 43,251 results for: cha;

1. pWR90

   Plasmid

   #203914

   Purpose

   empty BLINCAR plasmid for payload delivery, no payload, integrates at GSC2 locus

   Depositor

   J. Brian Robertson

   Article

   Reichard et al mSphere. 2023 Aug 24;8(4):e0022423. doi:

   Insert

   GSC2 plus flanking (GSC2 S. stipitis (yeast))
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   June 25, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pWR89

   Plasmid

   #203423

   Purpose

   empty BLINCAR plasmid for payload delivery, no payload, integrates at EGC3 locus

   Depositor

   J. Brian Robertson

   Article

   Reichard et al mSphere. 2023 Aug 24;8(4):e0022423. doi:

   Insert

   EGC3 plus flanking (EGC3 S. stipitis (yeast))
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   June 25, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. BS-ZJLEU-PTEF1-GFP

   Plasmid

   #207018

   Purpose

   Over-expression of GFP under TEF1 promoter, use auxotrophic marker LEU2(Saccharomyces cerevisiae).

   Depositor

   Zhiping Xie

   Article

   He et al BMC Biol. 2021 Jun 4;19(1):117. doi: 10.

   Insert

   GFP
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   pTEF1

   Available since

   June 12, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. PTPI1-Dsred-URA3

   Plasmid

   #207017

   Purpose

   Expresses DsRed-Express 2 as a marker of cytosol, uses auxotrophic marker URA3(Saccharomyces cerevisiae).

   Depositor

   Zhiping Xie

   Article

   He et al BMC Biol. 2021 Jun 4;19(1):117. doi: 10.

   Insert

   DsRed-Express 2
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   pTPI1

   Available since

   June 12, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pcDNA3.1-Hyg-nCas9n

   Plasmid

   #217487

   Purpose

   Expression of nuclear nCas9n

   Depositor

   Bertrand Collet

   Article

   Chaumont et al BMC Genomics. 2024 Jun 29;25(1):650. doi

   Insert

   Cas9
   

   Use

   CRISPR

   Tags

   SV40 NLS

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   April 19, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pGMC00029 (aka pRC0610)

   Plasmid

   #200834

   Purpose

   Lentiviral guide RNA expression plasmid for CRISPR activation with puromycin/BFP

   Depositor

   Raj Chari

   Article

   Yang et al Sci Adv. 2024 Feb 16;10(7):eadj2445. doi

   Type

   Empty backbone

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   April 5, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pMS18

   Plasmid

   #215675

   Purpose

   Cas9 + guide plasmid for inserting ChrI split hygromycinR landing pad

   Depositor

   Patrick Phillips

   Article

   Moerdyk-Schauwecker et al MicroPubl Biol. 2024 Jan 29;2024:10.1791

   Insert

   eef-1A.1p::Cas9 + U6p::GAAATCGCCGACTTGCGAGG
   

   Use

   CRISPR

   Tags

   Expression

   Worm

   Mutation

   Promoter

   Available since

   March 25, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pMS62

   Plasmid

   #215676

   Purpose

   Cas9 + guide plasmid for inserting ChrIII split hygromycinR landing pad

   Depositor

   Patrick Phillips

   Article

   Moerdyk-Schauwecker et al MicroPubl Biol. 2024 Jan 29;2024:10.1791

   Insert

   eef-1A.1p::Cas9 + U6p::GTCGTTCTTCCGTTCTCGGG
   

   Use

   CRISPR

   Tags

   Expression

   Worm

   Mutation

   Promoter

   Available since

   March 25, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pMS77

   Plasmid

   #215681

   Purpose

   Cas9 + guide plasmid targeting ChrIII split hygromycinR landing pad

   Depositor

   Patrick Phillips

   Article

   Moerdyk-Schauwecker et al MicroPubl Biol. 2024 Jan 29;2024:10.1791

   Insert

   eef-1A.1p::Cas9 + U6p::GTCCAGCGGCAGATCGGCGG
   

   Use

   CRISPR

   Tags

   Expression

   Worm

   Mutation

   Promoter

   Available since

   March 21, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. P1K-HFL1-8flag-LEU

    Plasmid

    #207035

    Purpose

    Expression of Hfl1-8Flag. Uses auxotrophic marker LEU2(Saccharomyces cerevisiae).

    Depositor

    Zhiping Xie

    Article

    He et al BMC Biol. 2021 Jun 4;19(1):117. doi: 10.

    Insert

    HFL1
    

    Use

    Tags

    8xFLAG

    Expression

    Yeast

    Mutation

    Promoter

    pHFL1

    Available since

    March 8, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pET SUMO-FL-syt7

    Plasmid

    #213630

    Purpose

    Bacterial expression of SUMO-tagged mouse FL-syt7

    Depositor

    Edwin Chapman

    Article

    Courtney et al Nat Commun. 2023 Nov 27;14(1):7761. doi:

    Insert

    FL-syt7 (Syt7 Mouse)
    

    Use

    Tags

    SUMO

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    March 6, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pkk223_TtMutY_F305A_S306A_H307A

    Plasmid

    #210793

    Purpose

    pkk223 with Thiotrichaceae MutY containing F305A, S306A, and H307A mutations to reduce recognition of OG:A

    Depositor

    Martin Horvath

    Article

    Utzman et al PLoS One. 2024 May 8;19(5):e0284642. doi

    Insert

    MutY (adenine glycosylase)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    F305A_S306A_H307A

    Promoter

    Available since

    Jan. 24, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pkk223_TtMutY_D147N_E46Q

    Plasmid

    #210792

    Purpose

    pkk223 with Thiotrichaceae MutY containing D147N and E46Q mutations to reduce catalytic activity

    Depositor

    Martin Horvath

    Article

    Utzman et al PLoS One. 2024 May 8;19(5):e0284642. doi

    Insert

    MutY (adenine glycosylase)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    D147N_E46Q

    Promoter

    Available since

    Jan. 24, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. NBla_1.2_GpA_G83I

    Plasmid

    #207844

    Purpose

    BLaTM-System GpA G83I negative control in NBLa 1.2 vector

    Depositor

    Dieter Langosch

    Article

    Schanzenbach et al Sci Rep. 2017 Mar 7;7:43476. doi: 10.103

    Insert

    N-BLa 1.2 fusion protein
    

    Use

    Tags

    Flag

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Jan. 10, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. NBla_1.2_GpA_wt

    Plasmid

    #207842

    Purpose

    BLaTM-System GpA wt positive control in NBLa 1.2 vector

    Depositor

    Dieter Langosch

    Article

    Schanzenbach et al Sci Rep. 2017 Mar 7;7:43476. doi: 10.103

    Insert

    N-BLa 1.2 fusion protein
    

    Use

    Tags

    Flag

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Dec. 21, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. CBLa_1.2_GpA_wt

    Plasmid

    #207843

    Purpose

    BLaTM-System GpA wt positive control in CBLa 1.2 vector

    Depositor

    Dieter Langosch

    Article

    Schanzenbach et al Sci Rep. 2017 Mar 7;7:43476. doi: 10.103

    Insert

    N-BLa 1.2 fusion protein
    

    Use

    Tags

    Flag

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Dec. 21, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. CBLa_1.2_GpA_G83I

    Plasmid

    #207845

    Purpose

    BLaTM-System GpA G83I negative control in CBLa 1.2 vector

    Depositor

    Dieter Langosch

    Article

    Schanzenbach et al Sci Rep. 2017 Mar 7;7:43476. doi: 10.103

    Insert

    N-BLa 1.2 fusion protein
    

    Use

    Tags

    Flag

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Dec. 21, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pCI pHluorin Syt6

    Plasmid

    #184507

    Purpose

    Mammalian expression of Synaptotagmin 6 with pH-sensitive fluorescent protein

    Depositor

    Edwin Chapman

    Article

    Dean et al Mol Biol Cell. 2012 May;23(9):1715-27. d

    Insert

    Synaptotagmin 6 (Syt6 Mouse)
    

    Use

    Tags

    Super Ecliptic pHluorin

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 14, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pETmsGFPSyt1(80 JuxtaK-142)

    Plasmid

    #209013

    Purpose

    Recombinant protein expression of truncated syt1 construct with Juxta K mutations and tagged with GFP

    Depositor

    Edwin Chapman

    Article

    Mehta et al Nat Commun. 2024 Jan 4;15(1):262. doi: 1

    Insert

    syt1 (Syt1 Rat)
    

    Use

    Tags

    6xHis-msfGFP

    Expression

    Bacterial

    Mutation

    aa 80-142 only, Juxta K mutant (80KKCLFKKKNKKKGKE…

    Promoter

    Available since

    Nov. 9, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pETSyt1C2AB(143-421)msGFP

    Plasmid

    #209010

    Purpose

    Recombinant protein expression of truncated syt1 tagged with GFP

    Depositor

    Edwin Chapman

    Article

    Mehta et al Nat Commun. 2024 Jan 4;15(1):262. doi: 1

    Insert

    syt1 (Syt1 Rat)
    

    Use

    Tags

    6xHis and msfGFP

    Expression

    Bacterial

    Mutation

    aa 143-421 only

    Promoter

    Available since

    Nov. 9, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pETSUMOSyt1C2AB(80 JuxtaK-421)

    Plasmid

    #209008

    Purpose

    Recombinant protein expression of truncated syt1 construct with Juxta K mutations

    Depositor

    Edwin Chapman

    Article

    Mehta et al Nat Commun. 2024 Jan 4;15(1):262. doi: 1

    Insert

    syt1 (Syt1 Rat)
    

    Use

    Tags

    6xHis-SUMO

    Expression

    Bacterial

    Mutation

    aa 80-421 only, Juxta K mutant (80KKCLFKKKNKKKGKE…

    Promoter

    Available since

    Nov. 9, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pFSynWSyt1C2AB(143-421)msGFP

    Plasmid

    #209015

    Purpose

    Overexpression of truncated syt1 tagged with GFP in mammalian cells

    Depositor

    Edwin Chapman

    Article

    Mehta et al Nat Commun. 2024 Jan 4;15(1):262. doi: 1

    Insert

    syt1 (Syt1 Rat)
    

    Use

    Lentiviral

    Tags

    msfGFP

    Expression

    Mammalian

    Mutation

    aa 143-421 only

    Promoter

    Available since

    Nov. 9, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. MsACR1-pmCerulean3-N1

    Plasmid

    #204958

    Purpose

    Expression of channelrhodopsin MsACR1 fused to mCerulean3 in mammalian cells

    Depositor

    Peter Hegemann

    Article

    Oppermann et al eLife. 2024 Oct 14;12:RP90100. doi: 10.7

    Insert

    MsACR1
    

    Use

    Tags

    mCerulean3

    Expression

    Mammalian

    Mutation

    Promoter

    CMV (+enhancer)

    Available since

    Oct. 25, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pGEX SYT9 C2A

    Plasmid

    #195707

    Purpose

    Bacterial expression plasmid encoding GST-tagged SYT9 C2A domain

    Depositor

    Edwin Chapman

    Article

    Seibert et al J Neurosci. 2023 Mar 1;43(9):1475-1491.

    Insert

    Syt9 (Syt5 Mouse)
    

    Use

    Tags

    GST

    Expression

    Bacterial

    Mutation

    encodes amino acids 124-211 of mouse SYT9

    Promoter

    Available since

    Oct. 16, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pGEX SYT9 C2B

    Plasmid

    #195708

    Purpose

    Bacterial expression plasmid encoding GST-tagged SYT9 C2B domain

    Depositor

    Edwin Chapman

    Article

    Seibert et al J Neurosci. 2023 Mar 1;43(9):1475-1491.

    Insert

    Syt9 (Syt5 Mouse)
    

    Use

    Tags

    GST

    Expression

    Bacterial

    Mutation

    encodes amino acids 253-344 of mouse SYT9

    Promoter

    Available since

    Oct. 16, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pGEX SYT9 C2AB

    Plasmid

    #195709

    Purpose

    Bacterial expression plasmid encoding GST-tagged SYT9 C2AB domain

    Depositor

    Edwin Chapman

    Article

    Seibert et al J Neurosci. 2023 Mar 1;43(9):1475-1491.

    Insert

    Syt9 (Syt5 Mouse)
    

    Use

    Tags

    GST

    Expression

    Bacterial

    Mutation

    encodes amino acids 104-386 of mouse SYT9

    Promoter

    Available since

    Oct. 16, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pGEX SYT1 C2A

    Plasmid

    #195710

    Purpose

    Bacterial expression plasmid encoding GST-tagged SYT1 C2A domain

    Depositor

    Edwin Chapman

    Article

    Seibert et al J Neurosci. 2023 Mar 1;43(9):1475-1491.

    Insert

    Syt1 (Syt1 Rat)
    

    Use

    Tags

    GST

    Expression

    Bacterial

    Mutation

    encodes amino acids 96-264 of mouse SYT1

    Promoter

    Available since

    Oct. 16, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pGEX SYT1 C2B

    Plasmid

    #195711

    Purpose

    Bacterial expression plasmid encoding GST-tagged SYT1 C2B domain

    Depositor

    Edwin Chapman

    Article

    Seibert et al J Neurosci. 2023 Mar 1;43(9):1475-1491.

    Insert

    Syt1 (Syt1 Rat)
    

    Use

    Tags

    GST

    Expression

    Bacterial

    Mutation

    encodes amino acids 248-421 of mouse SYT1

    Promoter

    Available since

    Oct. 16, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pGEX SYT1 C2AB

    Plasmid

    #195712

    Purpose

    Bacterial expression plasmid encoding GST-tagged SYT1 C2AB domain

    Depositor

    Edwin Chapman

    Article

    Seibert et al J Neurosci. 2023 Mar 1;43(9):1475-1491.

    Insert

    Syt1 (Syt1 Rat)
    

    Use

    Tags

    GST

    Expression

    Bacterial

    Mutation

    encodes amino acids 96-421 of mouse SYT1

    Promoter

    Available since

    Oct. 16, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pET (SUMO)-Syt9

    Plasmid

    #195713

    Purpose

    Bacterial expression plasmid encoding SUMO-tagged full-length Syt9

    Depositor

    Edwin Chapman

    Article

    Seibert et al J Neurosci. 2023 Mar 1;43(9):1475-1491.

    Insert

    Syt9 (Syt5 Rat)
    

    Use

    Tags

    GST-SUMO

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Oct. 16, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. pTet-Bxb1-Xis-mScarlet

    Plasmid

    #195570

    Purpose

    Expresses Bxb1 excisionase fused with mScarlet for fluorescence

    Depositor

    Richard Murray

    Article

    Pandey et al ACS Synth Biol. 2023 Feb 17;12(2):511-52

    Insert

    Bxb1 Excisionase Fused With mScarlet
    

    Use

    Synthetic Biology

    Tags

    mScarlet

    Expression

    Mutation

    Promoter

    P7

    Available since

    Aug. 4, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. Talin-T48-L432G-Cterminal-mEmerald

    Plasmid

    #202373

    Purpose

    Talin(DeltaR2-10)-L432G-mEmerald

    Depositor

    Pakorn Kanchanawong

    Article

    Liu et al Proc Natl Acad Sci U S A. 2015 Sep 1;112

    Insert

    Talin(DeltaR2-10)-L432G-mEmerald
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 1, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. Talin-T58-L432G-Cterminal-mEmerald

    Plasmid

    #202372

    Purpose

    Talin(DeltaR4-10)-L432G-mEmerald

    Depositor

    Pakorn Kanchanawong

    Article

    Liu et al Proc Natl Acad Sci U S A. 2015 Sep 1;112

    Insert

    Talin(DeltaR4-10)-L432G-mEmerald
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 1, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. Talin-T100-L432G-Cterminal-mEmerald

    Plasmid

    #202371

    Purpose

    Talin(FullLength)-L432G-mEmerald

    Depositor

    Pakorn Kanchanawong

    Article

    Liu et al Proc Natl Acad Sci U S A. 2015 Sep 1;112

    Insert

    Talin(FullLength)-L432G-mEmerald
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 1, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. Talin-T46-L432G-Cterminal-mEmerald

    Plasmid

    #202374

    Purpose

    Talin(DeltaR4-12)-L432G-mEmerald

    Depositor

    Pakorn Kanchanawong

    Article

    Liu et al Proc Natl Acad Sci U S A. 2015 Sep 1;112

    Insert

    Talin(DeltaR4-12)-L432G-mEmerald
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 1, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. Atg11 [699-800] pHis2

    Plasmid

    #190264

    Purpose

    Codon optimized Atg11 amino acids 699-800 from S. cerevisiae for expression in E. coli.

    Depositor

    Michael Ragusa

    Article

    Margolis et al J Mol Biol. 2020 Oct 2;432(21):5752-5764

    Insert

    ATG11
    

    Use

    Tags

    6xHis-TEV

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    July 12, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. Dcp2 H237A-EGFP

    Plasmid

    #201425

    Purpose

    Bacterial protein expression of Dcp2 H237A

    Depositor

    Michael Rosen

    Article

    Currie et al Proc Natl Acad Sci U S A. 2023 Apr 4;120

    Insert

    Dcp2 (DCP2 Budding Yeast)
    

    Use

    Tags

    EGFP, TEV, 6xHis and MBP, TEV

    Expression

    Bacterial

    Mutation

    H237A

    Promoter

    tac

    Available since

    June 9, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. Dhh1 25-425-EGFP

    Plasmid

    #201430

    Purpose

    Bacterial protein expression of Dhh1 25-425

    Depositor

    Michael Rosen

    Article

    Currie et al Proc Natl Acad Sci U S A. 2023 Apr 4;120

    Insert

    Dhh1 (DHH1 Budding Yeast)
    

    Use

    Tags

    EGFP, TEV, 6xHis and MBP, TEV

    Expression

    Bacterial

    Mutation

    aa 25-425 only

    Promoter

    tac

    Available since

    June 5, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. Dcp2 301-970-EGFP

    Plasmid

    #201429

    Purpose

    Bacterial protein expression of Dcp2 301-970

    Depositor

    Michael Rosen

    Article

    Currie et al Proc Natl Acad Sci U S A. 2023 Apr 4;120

    Insert

    Dcp2 (DCP2 Budding Yeast)
    

    Use

    Tags

    EGFP, TEV, 6xHis and MBP, TEV

    Expression

    Bacterial

    Mutation

    aa 301-970 only

    Promoter

    tac

    Available since

    June 5, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. Dhh1 426-506-EGFP

    Plasmid

    #201431

    Purpose

    Bacterial protein expression of Dhh1 426-506

    Depositor

    Michael Rosen

    Article

    Currie et al Proc Natl Acad Sci U S A. 2023 Apr 4;120

    Insert

    Dhh1 (DHH1 Budding Yeast)
    

    Use

    Tags

    EGFP, TEV, 6xHis and MBP, TEV

    Expression

    Bacterial

    Mutation

    aa 426-506 only

    Promoter

    tac

    Available since

    May 26, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge