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pEAHISSOarD1

Plasmid

#97067

Purpose

Expressing Sulfolobus solfataricus orf Sso Ard1 (N-terminal acetylase) protein

Depositor

Huanting Liu, James Naismith

Publication

Oke et al J Struct Funct Genomics. 2010 Jun;11(2):

Insert

S. solfataricus Sso Ard1 protein

Tags

N-terminal TEV protease cleavable 6xHis tag

Mutation

none

Promoter

T7

Available since

Dec. 14, 2017

Availability

Academic Institutions and Nonprofits only

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pLENTICRISPR-PGAM1-sgRNA1

Plasmid

#165943

Purpose

CRISPR-mediated gene perturbation (knock-out) of PGAM1

Depositor

Vamsi Mootha

Publication

Alexis A. et al Molecular Cell

Insert

PGAM1 (PGAM1 Human)

Use

Lentiviral

Promoter

U6

Availability

Academic Institutions and Nonprofits only

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pLENTICRISPR-PGAM1-sgRNA2

Plasmid

#165944

Purpose

CRISPR-mediated gene perturbation (knock-out) of PGAM1

Depositor

Vamsi Mootha

Publication

Alexis A. et al Molecular Cell

Insert

PGAM1 (PGAM1 Human)

Use

Lentiviral

Promoter

U6

Availability

Academic Institutions and Nonprofits only

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pLENTICRISPR-TPI1-sgRNA1

Plasmid

#165949

Purpose

CRISPR-mediated gene perturbation (knock-out) of TPI1

Depositor

Vamsi Mootha

Publication

Alexis A. et al Molecular Cell

Insert

TPI1 (TPI1 Human)

Use

Lentiviral

Promoter

U6

Availability

Academic Institutions and Nonprofits only

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pLENTICRISPR-TPI1-sgRNA2

Plasmid

#165950

Purpose

CRISPR-mediated gene perturbation (knock-out) of TPI1

Depositor

Vamsi Mootha

Publication

Alexis A. et al Molecular Cell

Insert

TPI1 (TPI1 Human)

Use

Lentiviral

Promoter

U6

Availability

Academic Institutions and Nonprofits only

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pCMV-Tag2B Flag BimS

Plasmid

#23283

Depositor

Roger Davis

Publication

Hubner et al Mol Cell. 2008 May 23. 30(4):415-25.

Insert

pCMV Tag2b Bim S (Bcl2l11 Mouse)

Tags

Flag

Expression

Mammalian

Available since

Feb. 26, 2010

Availability

Academic Institutions and Nonprofits only
SpyTag-RBD

Plasmid

#159999

Purpose

Expresses SARS-CoV-2 RBD (340-538) (receptor-binding domain from Spike) with N-terminal SpyTag fusion in mammalian cells

Depositor

Mark Howarth

Publication

Rahikainen et al Angew Chem Int Ed Engl. 2020 Sep 4. doi:

Insert

SpyTag-SARS-CoV-2 RBD (340-538) (S SARS-CoV-2)

Expression

Mammalian

Promoter

CMV

Available since

Nov. 9, 2020

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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pCAGGS D614G soluble Spike

Plasmid

#164651

Purpose

Expresses SARS-CoV-2 soluble Spike protein with a D614G mutation

Depositor

Jeremy Luban

Publication

Yurkovetskiy et al Cell. 2020 Sep 15. pii: S0092-8674(20)31

Insert

SARS-CoV-2 soluble spike (S SARS-CoV-2)

Tags

HIS

Expression

Mammalian

Mutation

D614G

Promoter

CAG

Available since

Feb. 16, 2021

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles
pssGFP-hCRT

Plasmid

#80022

Purpose

Mammalian expression of signal peptide-EGFP-humanCRT

Depositor

Chinten James Lim

Publication

Liu et al Cell Death Dis. 2016 Jun 16;7:e2268. doi

Insert

CRT (CALR Human)

Tags

EGFP and signal peptide

Expression

Mammalian

Mutation

signal peptide 17aa added before GFP-CRT, will ta…

Promoter

CMV

Available since

July 27, 2016

Availability

Academic Institutions and Nonprofits only
p413GPD-hTPI

Plasmid

#50719

Purpose

expression of human TPI1 in yeast

Depositor

Markus Ralser

Publication

Gruning et al Open Biol. 2014 Mar 5;4(3):130232. doi:

Insert

Triosephosphate isomerase 1 (TPI1 Human)

Expression

Yeast

Promoter

GPD

Available since

Jan. 30, 2014

Availability

Academic Institutions and Nonprofits only

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pSC-PreE2

Plasmid

#52264

Purpose

Bacterial expression of GST-tagged TARGET proteins fused to SUMO-E2 with PreScission protease cleavage site linker

Depositor

Primo Schaer

Publication

Weber et al PLoS One. 2014 Jul 9;9(7):e102157. doi:

Insert

Ubc9 GST fusion (UBE2I Human)

Tags

GST

Expression

Bacterial

Available since

July 10, 2014

Availability

Academic Institutions and Nonprofits only

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mycUBB+1

Plasmid

#20254

Depositor

Nico Dantuma

Publication

Verhoef et al FASEB J. 2009 Jan . 23(1):123-33.

Insert

Ubiquitin B +1 transcript (UBB Human)

Tags

myc

Expression

Mammalian

Available since

April 14, 2009

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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p176 Grg-1s Myc His

Plasmid

#11065

Depositor

Ramesh Shivdasani

Publication

Lepourcelet et al J Biol Chem. 2002 Dec 6. 277(49):47732-4

Insert

Grg1-S (Tle1 Mouse)

Tags

His and Myc

Expression

Mammalian

Available since

Jan. 5, 2006

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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huCof C139S-mRFP

Plasmid

#245969

Purpose

Expression of cof with one of 4 cys mutated to ser. Incorporates into cofilactin rods

Depositor

James Bamburg

Publication

Bernstein et al J Neurosci. 2012 May 9;32(19):6670-81. d

Insert

cofilin 1 (cfl1) (CFL1 Human)

Tags

mRFP

Expression

Mammalian

Mutation

C139S

Promoter

CMV

Available since

Oct. 22, 2025

Availability

Industry, Academic Institutions, and Nonprofits

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huCof C147A-mRFP

Plasmid

#245970

Purpose

Expression of cof with one of 4 cys mutated to ala .Incorporates into cofilactin rods

Depositor

James Bamburg

Publication

Bernstein et al J Neurosci. 2012 May 9;32(19):6670-81. d

Insert

cofilin 1 (cfl1) (CFL1 Human)

Tags

mRFP

Expression

Mammalian

Mutation

C147A

Promoter

CMV

Available since

Oct. 22, 2025

Availability

Industry, Academic Institutions, and Nonprofits

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huCof C147S-mRFP

Plasmid

#245971

Purpose

Expression of cof with one of 4 cys mutated to ser. Incorporates into cofilactin rods

Depositor

James Bamburg

Publication

Bernstein et al J Neurosci. 2012 May 9;32(19):6670-81. d

Insert

cofilin 1 (cfl1) (CFL1 Human)

Tags

mRFP

Expression

Mammalian

Mutation

C147S

Promoter

CMV

Available since

Oct. 22, 2025

Availability

Industry, Academic Institutions, and Nonprofits

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huCof C39A,C147A-mRFP

Plasmid

#245972

Purpose

Expression of cof with two of 4 cys mutated to A. Does NOT incorporate into cofilin-actin rods .

Depositor

James Bamburg

Publication

Bernstein et al J Neurosci. 2012 May 9;32(19):6670-81. d

Insert

cofilin 1 (cfl1) (CFL1 Human)

Tags

mRFP

Expression

Mammalian

Mutation

C39A,C147A

Promoter

CMV

Available since

Oct. 22, 2025

Availability

Industry, Academic Institutions, and Nonprofits

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huCof C39A-mRFP

Plasmid

#245966

Purpose

Expression of cof with one of 4 cys mutated to ala. Incorporates into cofilactin rods

Depositor

James Bamburg

Publication

Bernstein et al J Neurosci. 2012 May 9;32(19):6670-81. d

Insert

cofilin 1 (cfl1) (CFL1 Human)

Tags

mRFP

Expression

Mammalian

Mutation

C39A

Promoter

CMV

Available since

Oct. 22, 2025

Availability

Industry, Academic Institutions, and Nonprofits

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huCof C39S-mRFP

Plasmid

#245967

Purpose

Expression of cof with one of 4 cys mutated to ser. Incorporates into cofilactin rods

Depositor

James Bamburg

Publication

Bernstein et al J Neurosci. 2012 May 9;32(19):6670-81. d

Insert

cofilin 1 (cfl1) (CFL1 Human)

Tags

mRFP

Expression

Mammalian

Mutation

C39S

Promoter

CMV

Available since

Oct. 21, 2025

Availability

Industry, Academic Institutions, and Nonprofits

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huCof C139A-mRFP

Plasmid

#245968

Purpose

Expression of cof with one of 4 cys mutated to ala. Incorporates into cofilactin rods

Depositor

James Bamburg

Publication

Bernstein et al J Neurosci. 2012 May 9;32(19):6670-81. d

Insert

cofilin 1 (cfl1) (CFL1 Human)

Tags

mRFP

Expression

Mammalian

Mutation

C139A

Promoter

CMV

Available since

Oct. 21, 2025

Availability

Industry, Academic Institutions, and Nonprofits

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