We narrowed to 18,336 results for: met

1. pAAV-Syn-CoChR-GFP

   Plasmid

   #59070

   Purpose

   AAV-mediated expression of CoChR-GFP under the Syn promoter

   Depositor

   Edward Boyden

   Article

   Klapoetke et al Nat Methods. 2014 Mar;11(3):338-46. doi:

   Insert

   CoChR-GFP
   

   Use

   AAV

   Tags

   GFP

   Expression

   Mammalian

   Mutation

   Promoter

   Syn

   Available Since

   Aug. 14, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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2. pAAV-hSyn Coff/FonEYFP

   Plasmid

   #55652

   Purpose

   Cre-off/Flp-on EYFP under the Synapsin promoter

   Depositor

   Karl Deisseroth

   Article

   Fenno et al Nat Methods. 2014 Jul;11(7):763-72. doi:

   Insert

   eYFP with introns
   

   Use

   AAV; Cre off/flp on eyfp

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   hSyn

   Available Since

   Aug. 11, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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3. pGST-DHFR Mm

   Plasmid

   #83073

   Purpose

   Expresses the full length DHFR Mm in E.coli

   Depositor

   Gali Prag

   Article

   Levin-Kravets et al Nat Methods. 2016 Nov;13(11):945-952. do

   Insert

   2x DHFR (Dhfr Mouse)
   

   Use

   Tags

   GST

   Expression

   Bacterial

   Mutation

   Promoter

   Available Since

   Nov. 17, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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4. pAAV-CBh-IgKL-mNG

   Plasmid

   #183467

   Purpose

   To produce AAV with the CBh promoter to express IgkL-mNeonGreen (IgKL is a secretory signal peptide)

   Depositor

   Hajime Hirase

   Article

   Wang et al Cell Rep Methods. 2022 Sep 21;2(10):1003

   Insert

   IgKL-mNeonGreen
   

   Use

   AAV

   Tags

   IgKL secretory signal

   Expression

   Mutation

   Promoter

   Available Since

   May 11, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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5. pExodus CMV.Artemis

   Plasmid

   #40211

   Purpose

   Mammalian expression of codon-optimized Artemis.

   Depositor

   Andrew Scharenberg

   Article

   Certo et al Nat Methods. 2012 Oct;9(10):973-5. doi:

   Insert

   Artemis (DCLRE1C Human)
   

   Use

   Tags

   Expression

   Bacterial and Mammalian

   Mutation

   Promoter

   CMV and T7

   Available Since

   Sept. 27, 2012

   Availability

   Academic Institutions and Nonprofits only

   

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6. APEX2 - Actin in pEGFP

   Plasmid

   #66172

   Purpose

   APEX2 fused to the N-terminus of human beta actin

   Depositor

   Alice Ting

   Article

   Lam et al Nat Methods. 2014 Nov 24. doi: 10.1038/n

   Insert

   APEX2 - human beta Actin (ACTB Synthetic, Human)
   

   Use

   Tags

   Flag epitope tag (DYKDDDDK)

   Expression

   Mammalian

   Mutation

   APEX2 contains 4 mutations relative to wt soybean…

   Promoter

   CMV

   Available Since

   July 21, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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7. pCMV-DeAct-SpvB

   Plasmid

   #89446

   Purpose

   Expresses EGFP-SpvB (Salmonella SpvB 375-591) in mammalian cells

   Depositor

   Brad Zuchero

   Article

   Harterink et al Nat Methods. 2017 Apr 10. doi: 10.1038/n

   Insert

   DeAct-SpvB (spvB Salmonella enterica)
   

   Use

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   Truncation spanning SpvB amino acids 375-591 (mon…

   Promoter

   CMV

   Available Since

   April 27, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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8. pAS_4xEcoLeuT(CUA)_AnapRS

   Plasmid

   #140019

   Purpose

   Plasmid with 4xEcoLeuT(CUA) cassette and E. coli AnapRS for amber suppression and incorporation of the fluorescent ncAA Anap; for transient or stable piggyBac-mediated integration

   Depositor

   Simon Elsaesser

   Article

   Meineke et al Cell Rep Methods. 2023 Nov 20;3(11):1006

   Insert

   EcoLeuRS (AnapRS)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   L38F, M40G, L41P, Y499V, Y500L, Y527A, H537E, L53…

   Promoter

   EF1

   Available Since

   May 21, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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9. pTag-RFP-C-h-Rab11a

   Plasmid

   #79806

   Purpose

   Express TagRFP labeled human Rab11a (GTPase located on recycling endosomal membranes).

   Depositor

   James Johnson

   Article

   Boothe et al Mol Metab. 2016 Feb 10;5(5):366-78. doi:

   Insert

   Rab11a (RAB11A Human)
   

   Use

   Tags

   TagRFP

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   July 22, 2016

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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10. plentiCRISPR-v2 Puro EIF2AK3/PERK_sgRNA

    Plasmid

    #218666

    Purpose

    sgRNA targeting human EIF2AK3/PERK

    Depositor

    Kivanc Birsoy

    Article

    Kenny et al Cell Metab. 2023 Jun 6;35(6):1057-1071.e

    Insert

    EIF2AK3 (EIF2AK3 Human)
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    May 6, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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11. pMTS_mScarlet-i_N1

    Plasmid

    #85059

    Purpose

    In vivo visualization of the mitochondria (can be used for colocalization studies)

    Depositor

    Dorus Gadella

    Article

    Bindels et al Nat Methods. 2016 Nov 21. doi: 10.1038/n

    Insert

    MTS (COX8A Human)
    

    Use

    Tags

    mScarlet-i

    Expression

    Mammalian

    Mutation

    aa1-29

    Promoter

    CMV

    Available Since

    March 16, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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12. pOTTC590 - pCMV ER-iRFP

    Plasmid

    #59136

    Purpose

    A plasmid that express endoplasmic reticulum-targeted iRFP under the CMV promoter.

    Depositor

    Brandon Harvey

    Article

    Richie et al J Neurosci Methods. 2017 Jun 1;284:1-14.

    Insert

    ER-localized Infrared Fluorescent Protein
    

    Use

    Tags

    ER retention signal and signal peptide

    Expression

    Mammalian

    Mutation

    Promoter

    CMV-IE

    Available Since

    Oct. 4, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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13. plentiCRISPR-v2 Puro EIF2AK4/GCN2_sgRNA

    Plasmid

    #218528

    Purpose

    sgRNA targeting human EIF2AK4/GCN2

    Depositor

    Kivanc Birsoy

    Article

    Kenny et al Cell Metab. 2023 Jun 6;35(6):1057-1071.e

    Insert

    EIF2AK4 (EIF2AK4 Human)
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    May 6, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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14. pAAV-Ef1a-sCreDIO hChR2(H134R)-EYFP

    Plasmid

    #55642

    Purpose

    sCre-Dependent ChR2-EYFP

    Depositor

    Karl Deisseroth

    Article

    Fenno et al Nat Methods. 2014 Jul;11(7):763-72. doi:

    Insert

    hChR2(H134R)-EYFP
    

    Use

    AAV; Scre-dependent

    Tags

    EYFP

    Expression

    Mammalian

    Mutation

    Promoter

    Ef1a

    Available Since

    Aug. 8, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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15. FRB-TagRFPT-CAAX

    Plasmid

    #87710

    Purpose

    FRB-tagged TagRFPT; targeted to plasma membrane.

    Depositor

    Jin Zhang

    Article

    Mo et al Nat Methods. 2017 Apr;14(4):427-434. doi

    Insert

    FRB-TagRFPT-CAAX
    

    Use

    Tags

    6xHis, C-terminal targeting sequence from Kras, T…

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    Feb. 22, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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16. pMA7-SacB

    Plasmid

    #79968

    Purpose

    TM-MAGE strain

    Depositor

    Morten Sommer

    Article

    Lennen et al Nucleic Acids Res. 2016 Feb 29;44(4):e36

    Inserts

    Lambda Red recombinase beta subunit
    DNA adenine methylase
    Levansucrase
    

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    pBAD

    Available Since

    Sept. 2, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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17. pclbw-opa1(isoform 1)-myc

    Plasmid

    #62845

    Purpose

    Mammalian Expression of myc-tagged Opa1 isoform1

    Depositor

    David Chan

    Article

    Mishra et al Cell Metab. 2014 Apr 1;19(4):630-41. doi

    Insert

    mmOPA1isoform1-myc (Opa1 Mouse)
    

    Use

    Retroviral

    Tags

    9xMyc

    Expression

    Mammalian

    Mutation

    Promoter

    cmv-bactin

    Available Since

    Jan. 11, 2016

    Availability

    Academic Institutions and Nonprofits only

18. pMTS_mScarlet_N1

    Plasmid

    #85057

    Purpose

    In vivo visualization of the mitochondria (can be used for colocalization studies)

    Depositor

    Dorus Gadella

    Article

    Bindels et al Nat Methods. 2016 Nov 21. doi: 10.1038/n

    Insert

    MTS (COX8A Human)
    

    Use

    Tags

    mScarlet

    Expression

    Mammalian

    Mutation

    aa1-29

    Promoter

    CMV

    Available Since

    March 16, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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19. pmCherry-CRY2-CreN

    Plasmid

    #26888

    Purpose

    Expresses N-terminal fragment of Cre fused to CRY2 for blue light-induced activation of DNA recombination when used with CIBN-CreC. Also expresses mCherry.

    Depositor

    Chandra Tucker

    Article

    Kennedy et al Nat Methods. 2010 Dec;7(12):973-5. doi:

    Insert

    CRY2-CreN (cre, CRY2 Mustard Weed)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    aa 19-104 of Cre

    Promoter

    CMV

    Available Since

    Feb. 24, 2011

    Availability

    Academic Institutions and Nonprofits only

    

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20. YET1000: CAG-human dLbCpf1(D832A)-NLS-3xHA-3xFLAG-DmrA(X4)

    Plasmid

    #104571

    Purpose

    Mammalian expression vector for catalytically inactive Cpf1 from Lachnospiraceae bacterium (dLbCpf1) fused to four DmrA domains

    Depositor

    Keith Joung

    Article

    Tak et al Nat Methods. 2017 Oct 30. doi: 10.1038/n

    Insert

    human codon optimized ‘dead’ Cpf1 fused to four DmrA domains
    

    Use

    CRISPR

    Tags

    NLS-3xHA-3xFLAG-4xDmrA

    Expression

    Mammalian

    Mutation

    D832A

    Promoter

    CAG

    Available Since

    Jan. 17, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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