We narrowed to 31,526 results for: Eng;

1. pSMART-ala13

   Plasmid

   #87174

   Purpose

   NADPH dependent Alanine Dehydrogenase, Constitutive E. coli Expression

   Depositor

   Michael Lynch

   Article

   Ye et al Metab Eng. 2021 Nov;68:106-118. doi: 10.

   Insert

   NADPH Alanine Dehydrogenase (ald Synthetic)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   D196A, L197R

   Promoter

   Constitutive proD

   Available since

   June 9, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pSMART-ala11

   Plasmid

   #87172

   Purpose

   NADPH dependent Alanine Dehydrogenase, Constitutive E. coli Expression

   Depositor

   Michael Lynch

   Article

   Ye et al Metab Eng. 2021 Nov;68:106-118. doi: 10.

   Insert

   NADPH Alanine Dehydrogenase (ald Synthetic)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   D196A, L197R

   Promoter

   Constitutive proA

   Available since

   March 31, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pSMART-ala12

   Plasmid

   #87173

   Purpose

   NADPH dependent Alanine Dehydrogenase, Constitutive E. coli Expression

   Depositor

   Michael Lynch

   Article

   Ye et al Metab Eng. 2021 Nov;68:106-118. doi: 10.

   Insert

   NADPH Alanine Dehydrogenase (ald Synthetic)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   D196A, L197R

   Promoter

   Constitutive proC

   Available since

   March 27, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pETM6-Vv4CL-PhCHS-MsCHI

   Plasmid

   #73400

   Purpose

   Expresses three enzymes to convert phenylpropanoic acids to flavanones in vivo

   Depositor

   Mattheos Koffas

   Article

   Jones et al Metab Eng. 2016 Feb 6;35:55-63. doi: 10.

   Inserts

   Vv4CL
   PhCHS
   MsCHI
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   March 7, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pETM6-Vv4CL-CmCHS-CmCHI

   Plasmid

   #73403

   Purpose

   Expresses three enzymes to convert phenylpropanoic acids to flavanones in vivo

   Depositor

   Mattheos Koffas

   Article

   Jones et al Metab Eng. 2016 Feb 6;35:55-63. doi: 10.

   Inserts

   Vv4CL
   CmCHS
   CmCHI
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   March 1, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pETM6-Pc4CL-CmCHS-CmCHI

   Plasmid

   #73399

   Purpose

   Expresses three enzymes to convert phenylpropanoic acids to flavanones in vivo

   Depositor

   Mattheos Koffas

   Article

   Jones et al Metab Eng. 2016 Feb 6;35:55-63. doi: 10.

   Inserts

   Pc4CL
   CmCHS
   CmCHI
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   March 1, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pDSM-5a-Pact1-ITE-Tadh1

   Plasmid

   #127723

   Purpose

   Yeast pathway position 1. ITE transcription unit with the ACT1 promoter and ADH1 terminator.

   Depositor

   Christopher Voigt

   Article

   Young et al Metab Eng. 2018 Jul;48:33-43. doi: 10.10

   Insert

   itaconate transporter from A. terreus
   

   Use

   Synthetic Biology

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Pact1

   Available since

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pDSM-5a-Ppre3-ITE-Teno1

   Plasmid

   #127724

   Purpose

   Yeast pathway position 1. ITE transcription unit with the PRE3 promoter and ENO1 terminator.

   Depositor

   Christopher Voigt

   Article

   Young et al Metab Eng. 2018 Jul;48:33-43. doi: 10.10

   Insert

   itaconate transporter from A. terreus
   

   Use

   Synthetic Biology

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Ppre3

   Available since

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pDSM-5a-Pact1-ITE-Tdpp1

   Plasmid

   #127725

   Purpose

   Yeast pathway position 1. ITE transcription unit with the ACT1 promoter and DPP1 terminator.

   Depositor

   Christopher Voigt

   Article

   Young et al Metab Eng. 2018 Jul;48:33-43. doi: 10.10

   Insert

   itaconate transporter from A. terreus
   

   Use

   Synthetic Biology

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Pact1

   Available since

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pDSM-5a-Phta1-ITE-Tvma2

    Plasmid

    #127726

    Purpose

    Yeast pathway position 1. ITE transcription unit with the HTA1 promoter and VMA2 terminator.

    Depositor

    Christopher Voigt

    Article

    Young et al Metab Eng. 2018 Jul;48:33-43. doi: 10.10

    Insert

    itaconate transporter from A. terreus
    

    Use

    Synthetic Biology

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Phta1

    Available since

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pDSM-cd-Pfba1p-ACO-Tgpm1

    Plasmid

    #127731

    Purpose

    Yeast pathway position 4. ACO transcription unit with the FBA1 promoter and GPM1 terminator.

    Depositor

    Christopher Voigt

    Article

    Young et al Metab Eng. 2018 Jul;48:33-43. doi: 10.10

    Insert

    aconitase
    

    Use

    Synthetic Biology

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Pfba1

    Available since

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pDSM-cd-Pykt6-ACO-Tprm9

    Plasmid

    #127732

    Purpose

    Yeast pathway position 4. ACO transcription unit with the YKT6 promoter and PRM9 terminator.

    Depositor

    Christopher Voigt

    Article

    Young et al Metab Eng. 2018 Jul;48:33-43. doi: 10.10

    Insert

    aconitase
    

    Use

    Synthetic Biology

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Pykt6

    Available since

    Availability

    Academic Institutions and Nonprofits only

    

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13. pDSM-cd-Ptef1-ACO-Tyol036w

    Plasmid

    #127733

    Purpose

    Yeast pathway position 4. ACO transcription unit with the TEF1 promoter and YOL036w terminator.

    Depositor

    Christopher Voigt

    Article

    Young et al Metab Eng. 2018 Jul;48:33-43. doi: 10.10

    Insert

    aconitase
    

    Use

    Synthetic Biology

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Ptef1

    Available since

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pDSM-ef-Ptef1-CSC-Tpdc1

    Plasmid

    #127739

    Purpose

    Yeast pathway position 6. CSC transcription unit with the TEF1 promoter and PDC1 terminator.

    Depositor

    Christopher Voigt

    Article

    Young et al Metab Eng. 2018 Jul;48:33-43. doi: 10.10

    Insert

    citrate synthase
    

    Use

    Synthetic Biology

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Pfba1

    Available since

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pDSM-ef-Pzuo1-CSC-Tvma16

    Plasmid

    #127740

    Purpose

    Yeast pathway position 6. CSC transcription unit with the ZUO1 promoter and VMA16 terminator.

    Depositor

    Christopher Voigt

    Article

    Young et al Metab Eng. 2018 Jul;48:33-43. doi: 10.10

    Insert

    citrate synthase
    

    Use

    Synthetic Biology

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Pzuo1

    Available since

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pDSM-ef-Ppfy1-CSC-Taip1

    Plasmid

    #127741

    Purpose

    Yeast pathway position 6. CSC transcription unit with the PFY1 promoter and AIP1 terminator.

    Depositor

    Christopher Voigt

    Article

    Young et al Metab Eng. 2018 Jul;48:33-43. doi: 10.10

    Insert

    citrate synthase
    

    Use

    Synthetic Biology

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Ppfy1

    Available since

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. evoBxb1

    Plasmid

    #222338

    Purpose

    Plasmid for expressing evolved Bxb1 in the mammalian cells

    Depositor

    David Liu

    Article

    Pandey et al Nat Biomed Eng. 2024 Jun 10. doi: 10.103

    Insert

    Codon-optimized evolved Bxb1
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    June 27, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. CAG-mCherry

    Plasmid

    #108685

    Purpose

    Strong CAG promoter with mCherry expression for transfection efficacy screening

    Depositor

    Jordan Green

    Article

    Mishra et al Regen Eng Transl Med. 2019 Sep;6(3):273-

    Insert

    mCherry
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available since

    Sept. 21, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. amilCP chromoprotein

    Plasmid

    #117847

    Purpose

    BioBrick pSB1C3 plasmid that constitutively overexpresses amilCP chromoprotein in E. coli

    Depositor

    Anthony Forster

    Article

    Liljeruhm et al J Biol Eng. 2018 May 10;12:8. doi: 10.11

    Insert

    promoter, RBS, amilCP
    

    Use

    Synthetic Biology; Escherichia coli

    Tags

    Expression

    Mutation

    Promoter

    Available since

    July 31, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. amilCP chromoprotein promoter-less

    Plasmid

    #118008

    Purpose

    For course in "Synthetic Biology: A Lab Manual"

    Depositor

    Anthony Forster

    Article

    Liljeruhm et al J Biol Eng. 2018 May 10;12:8. doi: 10.11

    Insert

    RBS, amilCP
    

    Use

    Synthetic Biology; Escherichia coli

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Aug. 1, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pREDCas9

    Plasmid

    #71541

    Purpose

    Constitutive expression of Cas9, inducible expression of the Red recombineering system, and inducible expression of the plasmid curing system for CRISPR mediated genome editing of E. coli.

    Depositor

    Tao Chen

    Article

    Li et al Metab Eng. 2015 Sep;31:13-21. doi: 10.10

    Inserts

    Cas9
    lambda red genes
    plasmid curing system
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    pBAD

    Available since

    July 29, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pBS1C

    Plasmid

    #55168

    Purpose

    Empty vector, integration at amyE, ampr, cmr

    Depositor

    Thorsten Mascher

    Article

    Radeck et al J Biol Eng. 2013 Dec 2;7(1):29. doi: 10.

    Type

    Empty backbone

    Use

    Synthetic Biology; Bacillus biobrick box

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Aug. 6, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. Circular-oc-CVB3-GFP

    Plasmid

    #226262

    Purpose

    Production of in vitro circularizable RNA via IVT

    Depositor

    Prashant Mali

    Article

    Tong et al Nat Biomed Eng. 2024 Aug 26. doi: 10.103

    Insert

    EGFP
    

    Use

    In vitro transcription

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Oct. 16, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pCA24N-p50-ligase

    Plasmid

    #87742

    Purpose

    IPTG-inducible expression of p50-ligase fusion for protein purification

    Depositor

    Wayne Patrick

    Article

    Wilson et al Protein Eng Des Sel. 2013 Jul;26(7):471-

    Insert

    T4 DNA ligase (30 )
    

    Use

    Tags

    6xHis and human p50/NF-kB (amino acids 40-366)

    Expression

    Bacterial

    Mutation

    Promoter

    T5-lac

    Available since

    March 21, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pCOLA-Gent-EM7-Erv1p-PDI

    Plasmid

    #202486

    Purpose

    Expresses sulfhydryl oxidase (Erv1p) and protein disulfide bond isomerase (PDI) constitutively

    Depositor

    Michael Lynch

    Article

    Hennigan et al Metab Eng. 2024 Sep;85:116-130. doi: 10.

    Insert

    sulfhydryl oxidase (Erv1p) and protein disulfide bond isomerase (PDI) (ERV1 Budding Yeast, Human)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    EM7

    Available since

    Nov. 14, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. Pveg

    Plasmid

    #55173

    Purpose

    Very strong constitutive promoter

    Depositor

    Thorsten Mascher

    Article

    Radeck et al J Biol Eng. 2013 Dec 2;7(1):29. doi: 10.

    Insert

    veg promoter
    

    Use

    Synthetic Biology; Bacillus biobrick box

    Tags

    Expression

    Mutation

    Promoter

    veg

    Available since

    July 7, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. evoBxb1_IVT

    Plasmid

    #222341

    Purpose

    Plasmid for evolved Bxb1 mRNA in vitro transcription template

    Depositor

    David Liu

    Article

    Pandey et al Nat Biomed Eng. 2024 Jun 10. doi: 10.103

    Insert

    Codon-optimized evolved Bxb1
    

    Use

    Template for in vitro transcription

    Tags

    Expression

    Mutation

    Promoter

    Available since

    July 25, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. Linear-GFP

    Plasmid

    #226258

    Purpose

    Production of linear RNA via IVT

    Depositor

    Prashant Mali

    Article

    Tong et al Nat Biomed Eng. 2024 Aug 26. doi: 10.103

    Insert

    EGFP
    

    Use

    In vitro transcription

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Oct. 16, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pCA24N-sso7d-ligase

    Plasmid

    #87745

    Purpose

    IPTG-inducible expression of sso7d-ligase fusion for protein purification

    Depositor

    Wayne Patrick

    Article

    Wilson et al Protein Eng Des Sel. 2013 Jul;26(7):471-

    Insert

    T4 DNA ligase (30 )
    

    Use

    Tags

    6xHis and codon optimized sso7d (Sulfolobus solfa…

    Expression

    Bacterial

    Mutation

    Promoter

    T5-lac

    Available since

    March 21, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. Circular-ic-CVB3-GFP

    Plasmid

    #226260

    Purpose

    Production of in situ circularizable RNA via IVT

    Depositor

    Prashant Mali

    Article

    Tong et al Nat Biomed Eng. 2024 Aug 26. doi: 10.103

    Insert

    EGFP
    

    Use

    In vitro transcription

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Oct. 16, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. Circular-ic-EMCV-GFP

    Plasmid

    #226259

    Purpose

    Production of in situ circularizable RNA via IVT

    Depositor

    Prashant Mali

    Article

    Tong et al Nat Biomed Eng. 2024 Aug 26. doi: 10.103

    Insert

    EGFP
    

    Use

    In vitro transcription

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Oct. 16, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. Keasling-2337

    Plasmid

    #100168

    Purpose

    Used to improve production from engineered biosynthetic pathways include optimizing codon usage, enhancing production of rate-limiting enzymes, and eliminating the accumulation of toxic intermediates or byproducts to improve cell growth

    Depositor

    Jay Keasling

    Article

    Anthony et al Metab Eng. 2009 Jan;11(1):13-9. doi: 10.

    Insert

    lacIq-Ptrc-ADS
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    trc

    Available since

    Sept. 15, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. PxylA

    Plasmid

    #55178

    Purpose

    Xylose-inducible promoter

    Depositor

    Thorsten Mascher

    Article

    Radeck et al J Biol Eng. 2013 Dec 2;7(1):29. doi: 10.

    Insert

    xylA promoter
    

    Use

    Synthetic Biology; Bacillus biobrick box

    Tags

    Expression

    Mutation

    Promoter

    xylA

    Available since

    July 22, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. wtBxb1_IVT

    Plasmid

    #222340

    Purpose

    Plasmid for wild type Bxb1 mRNA in vitro transcription template

    Depositor

    David Liu

    Article

    Pandey et al Nat Biomed Eng. 2024 Jun 10. doi: 10.103

    Insert

    Codon-optimized wtBxb1
    

    Use

    Template for in vitro transcription

    Tags

    Expression

    Mutation

    Promoter

    T7 (inactivated)

    Available since

    June 27, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. pCHC042 (∆phaCAB)

    Plasmid

    #226208

    Purpose

    Conjugation knockout plasmid (pK18msB) to delete the C. necator phaCAB operon, to prevent accumulation of PHB.

    Depositor

    Christopher Johnson

    Article

    Calvey et al Metab Eng. 2023 Jan;75:78-90. doi: 10.10

    Insert

    Homology arms for phaCAB
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Nov. 18, 2024

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

36. pETM6-At4CL-PhCHS-CmCHI (C5 mutant, pFlavo-opt)

    Plasmid

    #73404

    Purpose

    Expresses three enzymes to convert phenylpropanoic acids to flavanones in vivo

    Depositor

    Mattheos Koffas

    Article

    Jones et al Metab Eng. 2016 Feb 6;35:55-63. doi: 10.

    Inserts

    At4CL
    PhCHS
    CmCHI
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    March 1, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. pFNC-3

    Plasmid

    #233296

    Purpose

    Plasmid encoding the BxbI phage integrase. It can be used to remove the antibiotic resistance cassette integrated with pCIFR. CmR, easily curable via sucrose counterselection.

    Depositor

    Pablo Ivan Nikel

    Article

    Federici et al Metab Eng. 2025 Jan 6;88:180-195. doi: 1

    Inserts

    BxbI phage integrase
    sacB
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    PEM7 and unknown

    Available since

    April 3, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. Circular-oc-EMCV-GFP

    Plasmid

    #226261

    Purpose

    Production of in vitro circularizable RNA via IVT

    Depositor

    Prashant Mali

    Article

    Tong et al Nat Biomed Eng. 2024 Aug 26. doi: 10.103

    Insert

    EGFP
    

    Use

    In vitro transcription

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Oct. 16, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. pTS2340_Tier3(SB)-iRFP_Puro

    Plasmid

    #169638

    Purpose

    Tier-3 vector for stable stable integration of up to three cassettes via sleeping beauty transposase including a iRFP670 marker and PuroR resistance gene (5'ITR-A1-pA::A2-pA::PRPBSA-iRFP670-p2A-PuroR-pA-3'ITR)

    Depositor

    Martin Fussenegger

    Article

    Haellman et al Metab Eng. 2021 Jul;66:41-50. doi: 10.10

    Insert

    PRPBSA-driven iRFP670 and PuroR expression
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    PRPBSA

    Available since

    June 22, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. pZ_P-GAP-eGFP

    Plasmid

    #126717

    Purpose

    yeast plasmid overexpressing eGFP

    Depositor

    Menghao Cai

    Article

    Liu et al Metab Eng. 2019 Jul;54:275-284. doi: 10.

    Insert

    egfp
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    GAP

    Available since

    June 28, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge