Skip to main content
Addgene
Showing: 1051 - 1100 of 158806 results

  1. pEMS1986

    Plasmid
    #49117
    Purpose
    The plasmid contains the Pleaides (Ple) MiniPromoter Ple155 derived from the human PCP2 gene and designed for expression in ON Bipolar cells of the retina.
    Depositor
    Elizabeth Simpson
    Article
    Korecki et al Gene Ther. 2021 Feb 2. pii: 10.1038/s414
    Insert
    ssAAV-Ple155-iCre-WPRE
    Use
    AAV
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    MiniPromoter Ple155 from the human PCP2 gene
    Available since
    May 10, 2016
    Availability
    Industry, Academic Institutions, and Nonprofits
    49117-plasmid-map-sequence-id-73215

    Enlarge

  2. pUC19-E3CY56

    Plasmid
    #103075
    Purpose
    Cas9 coding gene template with optimized sequence for human codon usage
    Depositor
    Duhee Bang
    Article
    Cho et al Nucleic Acids Res. 2018 May 18;46(9):e55
    Insert
    E3CY56(Cas9 coding gene from Aminomonas paucivorans DSM 12260)
    Use
    CRISPR; Cloning vector
    Tags
    Expression
    Mutation
    human codon-optimized
    Promoter
    Available since
    April 20, 2018
    Availability
    Academic Institutions and Nonprofits only
    103075-plasmid-map-sequence-id-201714

    Enlarge

  5. pUDP012

    Plasmid
    #101167
    Purpose
    E. coli/S. cerevisiae shuttle vector carrying amdS andSpcas9D147Y P411T and expressing a ribozyme flanked g-RNA for Cas9 editing targeting the gene SeILV6 and in S. pastorianus (HH-gRNASeILV6-HDV)
    Depositor
    Jean-Marc Daran
    Article
    de Vries et al Microb Cell Fact. 2017 Dec 5;16(1):222.
    Insert
    HH-gRNA-HDV targetting SeILV6 in S. pastorianus
    Use
    CRISPR
    Tags
    Expression
    Yeast
    Mutation
    Promoter
    ScTDH3
    Available since
    Dec. 4, 2017
    Availability
    Academic Institutions and Nonprofits only
    101167-plasmid-map-sequence-id-199268

    Enlarge

  7. pMH012_AAVS1_puro-pA-9xTetO-pEF-H2B-mCitrine-core-pRSV-H2B-mCherry

    Plasmid
    #179435
    Purpose
    Dual fluorescent reporter construct with single core HS4 insulator, upstream TRE (Tetracycline Response Element) recruitment site, arms for integration at AAVS1 locus
    Depositor
    Lacra Bintu
    Article
    Lensch et al Elife. 2022 Jun 9;11. pii: 75115. doi: 1
    Insert
    TRE-cit-coreHS4-mch (SC)
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    pEF alpha, pRSV
    Available since
    Feb. 28, 2022
    Availability
    Academic Institutions and Nonprofits only
    179435-plasmid-map-sequence-id-355551

    Enlarge

  9. pUC19-E6MBW6

    Plasmid
    #103102
    Purpose
    Cas9 coding gene template with optimized sequence for human codon usage
    Depositor
    Duhee Bang
    Article
    Cho et al Nucleic Acids Res. 2018 May 18;46(9):e55
    Insert
    E6MBW6(Cas9 coding gene from Staphylococcus lugdunensis M23590)
    Use
    CRISPR; Cloning vector
    Tags
    Expression
    Mutation
    human codon-optimized
    Promoter
    Available since
    April 24, 2018
    Availability
    Academic Institutions and Nonprofits only
    103102-plasmid-map-sequence-id-209054

    Enlarge

  12. pGEM-cytoBirA-2A-mCherry-SV40pA-FKF

    Plasmid
    #79887
    Purpose
    Donor cassette containing HA-tagged biotin ligase (BirA), a Thosea asigna virus peptide (2A), mCherry protein and SV40 polyA tail, followed by FRT site-flanked Kanamycin selection gene
    Depositor
    Tatjana Sauka-Spengler
    Article
    Trinh et al Cell Rep. 2017 Apr 11;19(2):425-440. doi
    Insert
    HA-tagged BirA, 2A, mCherry protein and SV40 polyA, followed by FRT site-flanked Kanamycin selection gene
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    Oct. 11, 2016
    Availability
    Academic Institutions and Nonprofits only
    79887-plasmid-map-sequence-id-402553

    Enlarge

  15. pUC19-G2ZYP2

    Plasmid
    #103110
    Purpose
    Cas9 coding gene template with optimized sequence for human codon usage
    Depositor
    Duhee Bang
    Article
    Cho et al Nucleic Acids Res. 2018 May 18;46(9):e55
    Insert
    G2ZYP2(Cas9 coding gene from Ralstonia syzygii R24)
    Use
    CRISPR; Cloning vector
    Tags
    Expression
    Mutation
    human codon-optimized
    Promoter
    Available since
    April 20, 2018
    Availability
    Academic Institutions and Nonprofits only
    103110-plasmid-map-sequence-id-201819

    Enlarge

  16. pUC19-Q927P4

    Plasmid
    #103131
    Purpose
    Cas9 coding gene template with optimized sequence for human codon usage
    Depositor
    Duhee Bang
    Article
    Cho et al Nucleic Acids Res. 2018 May 18;46(9):e55
    Insert
    Q927P4(Cas9 coding gene from Listeria innocua serovar 6a (strain CLIP 11262))
    Use
    CRISPR; Cloning vector
    Tags
    Expression
    Mutation
    human codon-optimized
    Promoter
    Available since
    April 20, 2018
    Availability
    Academic Institutions and Nonprofits only
    103131-plasmid-map-sequence-id-201895

    Enlarge

  24. pSL012_AAVS1-puro-9xTetO-pEF-H2B-mCitrine-5kb,lambda-pRSV-H2B-mCherry

    Plasmid
    #179429
    Purpose
    Dual fluorescent reporter construct with 5kb lambda spacer, upstream TRE (Tetracycline Response Element) recruitment site, arms for integration at AAVS1 locus
    Depositor
    Lacra Bintu
    Article
    Lensch et al Elife. 2022 Jun 9;11. pii: 75115. doi: 1
    Insert
    TRE-cit-5kb-mch
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    pEF alpha, pRSV
    Available since
    Feb. 28, 2022
    Availability
    Academic Institutions and Nonprofits only
    179429-plasmid-map-sequence-id-355448

    Enlarge

  27. pCAG-B2UP49_GFP

    Plasmid
    #103071
    Purpose
    Cas9 coding gene template with optimized sequence for human codon usage, also expresses EGFP
    Depositor
    Duhee Bang
    Article
    Cho et al Nucleic Acids Res. 2018 May 18;46(9):e55
    Insert
    B2UP49(Cas9 coding gene from Akkermansia muciniphila (strain ATCC BAA-835))
    Use
    CRISPR
    Tags
    Expression
    Mammalian
    Mutation
    human codon-optimized
    Promoter
    CMV
    Available since
    April 20, 2018
    Availability
    Academic Institutions and Nonprofits only
    103071-plasmid-map-sequence-id-201896

    Enlarge

  28. pUDP044

    Plasmid
    #101168
    Purpose
    pUDP004 expressing a polycistronic g-RNA array for Cas9 editing targeting the genes SeATF1 and SeATF2 and Spcas9D147Y P411T in S. pastorianus (HH-gRNASeATF1-HDV-linker-HH-gRNASeATF2-HDV)
    Depositor
    Jean-Marc Daran
    Article
    de Vries et al Microb Cell Fact. 2017 Dec 5;16(1):222.
    Insert
    polycistronic HH-gRNA-HDV-HH-gRNA-HDV array targetting SeATF1 and 2 in S. pastorianus
    Use
    CRISPR
    Tags
    Expression
    Yeast
    Mutation
    Promoter
    ScTDH3
    Available since
    Dec. 4, 2017
    Availability
    Academic Institutions and Nonprofits only
    101168-plasmid-map-sequence-id-199309

    Enlarge

  29. pEMS2116

    Plasmid
    #49141
    Purpose
    The plasmid contains the Pleaides (Ple) MiniPromoter Ple155 derived from the human PCP2 gene and designed for expression in ON Bipolar cells of the retina.
    Depositor
    Elizabeth Simpson
    Article
    Korecki et al Gene Ther. 2021 Feb 2. pii: 10.1038/s414
    Insert
    ssAAV-Ple155-emGFP
    Use
    AAV
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    MiniPromoter Ple155 from the human PCP2 gene
    Available since
    May 10, 2016
    Availability
    Industry, Academic Institutions, and Nonprofits
    49141-plasmid-map-sequence-id-73242

    Enlarge

  30. pUC19-A0LWB3

    Plasmid
    #103070
    Purpose
    Cas9 coding gene template with optimized sequence for human codon usage
    Depositor
    Duhee Bang
    Article
    Cho et al Nucleic Acids Res. 2018 May 18;46(9):e55
    Insert
    A0LWB3(Cas9 coding gene from Acidothermus cellulolyticus (strain ATCC 43068 / 11B))
    Use
    CRISPR; Cloning vector
    Tags
    Expression
    Mutation
    human codon-optimized
    Promoter
    Available since
    April 20, 2018
    Availability
    Academic Institutions and Nonprofits only
    103070-plasmid-map-sequence-id-201692

    Enlarge

  32. pUC19-K1LM04

    Plasmid
    #103119
    Purpose
    Cas9 coding gene template with optimized sequence for human codon usage
    Depositor
    Duhee Bang
    Article
    Cho et al Nucleic Acids Res. 2018 May 18;46(9):e55
    Insert
    K1LM04(Cas9 coding gene from Bergeyella zoohelcum ATCC 43767)
    Use
    CRISPR; Cloning vector
    Tags
    Expression
    Mutation
    human codon-optimized
    Promoter
    Available since
    April 20, 2018
    Availability
    Academic Institutions and Nonprofits only
    103119-plasmid-map-sequence-id-201919

    Enlarge

  33. pUC19-E6I4Q5

    Plasmid
    #103101
    Purpose
    Cas9 coding gene template with optimized sequence for human codon usage
    Depositor
    Duhee Bang
    Article
    Cho et al Nucleic Acids Res. 2018 May 18;46(9):e55
    Insert
    E6I4Q5(Cas9 coding gene from Enterococcus faecalis TX0012)
    Use
    CRISPR; Cloning vector
    Tags
    Expression
    Mutation
    human codon-optimized
    Promoter
    Available since
    April 20, 2018
    Availability
    Academic Institutions and Nonprofits only
    103101-plasmid-map-sequence-id-201810

    Enlarge

  34. pGEM-NLS-BirA-2A-mCherry-SV40pA-FKF

    Plasmid
    #79889
    Purpose
    Donor cassette containing HA-tagged biotin ligase (BirA) with NLS, a Thosea asigna virus peptide (2A), mCherry protein and SV40 polyA tail, followed by FRT site-flanked Kanamycin selection gene
    Depositor
    Tatjana Sauka-Spengler
    Article
    Trinh et al Cell Rep. 2017 Apr 11;19(2):425-440. doi
    Insert
    HA-tagged NLS-BirA, 2A, mCherry protein and SV40 polyA, followed by FRT site-flanked Kanamycin selection gene
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    Oct. 11, 2016
    Availability
    Academic Institutions and Nonprofits only
    79889-plasmid-map-sequence-id-150065

    Enlarge

  36. pUC19-F3UXG6

    Plasmid
    #103108
    Purpose
    Cas9 coding gene template with optimized sequence for human codon usage
    Depositor
    Duhee Bang
    Article
    Cho et al Nucleic Acids Res. 2018 May 18;46(9):e55
    Insert
    F3UXG6(Cas9 coding gene from Streptococcus sanguinis SK49)
    Use
    CRISPR; Cloning vector
    Tags
    Expression
    Mutation
    human codon-optimized
    Promoter
    Available since
    April 20, 2018
    Availability
    Academic Institutions and Nonprofits only
    103108-plasmid-map-sequence-id-201817

    Enlarge

  38. pUC19-C7TEQ6

    Plasmid
    #103092
    Purpose
    Cas9 coding gene template with optimized sequence for human codon usage
    Depositor
    Duhee Bang
    Article
    Cho et al Nucleic Acids Res. 2018 May 18;46(9):e55
    Insert
    C7TEQ6(Cas9 coding gene from Lactobacillus rhamnosus (strain ATCC 53103 / GG))
    Use
    CRISPR; Cloning vector
    Tags
    Expression
    Mutation
    human codon-optimized
    Promoter
    Available since
    April 20, 2018
    Availability
    Academic Institutions and Nonprofits only
    103092-plasmid-map-sequence-id-201803

    Enlarge

  39. pUC19-D4J3S7

    Plasmid
    #103096
    Purpose
    Cas9 coding gene template with optimized sequence for human codon usage
    Depositor
    Duhee Bang
    Article
    Cho et al Nucleic Acids Res. 2018 May 18;46(9):e55
    Insert
    D4J3S7(Cas9 coding gene from Coprococcus catus GD/7)
    Use
    CRISPR; Cloning vector
    Tags
    Expression
    Mutation
    human codon-optimized
    Promoter
    Available since
    April 20, 2018
    Availability
    Academic Institutions and Nonprofits only
    103096-plasmid-map-sequence-id-201806

    Enlarge

  40. pUC19-E3HCA8

    Plasmid
    #103100
    Purpose
    Cas9 coding gene template with optimized sequence for human codon usage
    Depositor
    Duhee Bang
    Article
    Cho et al Nucleic Acids Res. 2018 May 18;46(9):e55
    Insert
    E3HCA8(Cas9 coding gene from Ilyobacter polytropus (strain DSM 2926 / CuHBu1))
    Use
    CRISPR; Cloning vector
    Tags
    Expression
    Mutation
    human codon-optimized
    Promoter
    Available since
    April 24, 2018
    Availability
    Academic Institutions and Nonprofits only
    103100-plasmid-map-sequence-id-209053

    Enlarge

  41. pUC19-A8REH9

    Plasmid
    #103084
    Purpose
    Cas9 coding gene template with optimized sequence for human codon usage
    Depositor
    Duhee Bang
    Article
    Cho et al Nucleic Acids Res. 2018 May 18;46(9):e55
    Insert
    A8REH9(Cas9 coding gene from Eubacterium dolichum DSM 3991)
    Use
    CRISPR; Cloning vector
    Tags
    Expression
    Mutation
    human codon-optimized
    Promoter
    Available since
    April 24, 2018
    Availability
    Academic Institutions and Nonprofits only
    103084-plasmid-map-sequence-id-209043

    Enlarge

  42. pUC19-J6LE60

    Plasmid
    #103117
    Purpose
    Cas9 coding gene template with optimized sequence for human codon usage
    Depositor
    Duhee Bang
    Article
    Cho et al Nucleic Acids Res. 2018 May 18;46(9):e55
    Insert
    J6LE60(Cas9 coding gene from Rhodovulum sp. PH10)
    Use
    CRISPR; Cloning vector
    Tags
    Expression
    Mutation
    human codon-optimized
    Promoter
    Available since
    April 20, 2018
    Availability
    Academic Institutions and Nonprofits only
    103117-plasmid-map-sequence-id-201879

    Enlarge

  43. pUC19-Q0P897

    Plasmid
    #103120
    Purpose
    Cas9 coding gene template with optimized sequence for human codon usage
    Depositor
    Duhee Bang
    Article
    Cho et al Nucleic Acids Res. 2018 May 18;46(9):e55
    Insert
    Q0P897(Cas9 coding gene from Rhodospirillum rubrum (strain ATCC 11170 / NCIB 8255))
    Use
    CRISPR; Cloning vector
    Tags
    Expression
    Mutation
    human codon-optimized
    Promoter
    Available since
    April 20, 2018
    Availability
    Academic Institutions and Nonprofits only
    103120-plasmid-map-sequence-id-201882

    Enlarge

  44. pUC19-Q4A5I2

    Plasmid
    #103125
    Purpose
    Cas9 coding gene template with optimized sequence for human codon usage
    Depositor
    Duhee Bang
    Article
    Cho et al Nucleic Acids Res. 2018 May 18;46(9):e55
    Insert
    Q4A5I2(Cas9 coding gene from Mycoplasma synoviae (strain 53))
    Use
    CRISPR; Cloning vector
    Tags
    Expression
    Mutation
    human codon-optimized
    Promoter
    Available since
    April 20, 2018
    Availability
    Academic Institutions and Nonprofits only
    103125-plasmid-map-sequence-id-201888

    Enlarge

  45. pUC19-Q5X8T6

    Plasmid
    #103127
    Purpose
    Cas9 coding gene template with optimized sequence for human codon usage
    Depositor
    Duhee Bang
    Article
    Cho et al Nucleic Acids Res. 2018 May 18;46(9):e55
    Insert
    Q5X8T6(Cas9 coding gene from Legionella pneumophila (strain Paris))
    Use
    CRISPR; Cloning vector
    Tags
    Expression
    Mutation
    human codon-optimized
    Promoter
    Available since
    April 20, 2018
    Availability
    Academic Institutions and Nonprofits only
    103127-plasmid-map-sequence-id-201890

    Enlarge

  46. pUC19-C7G697

    Plasmid
    #103091
    Purpose
    Cas9 coding gene template with optimized sequence for human codon usage
    Depositor
    Duhee Bang
    Article
    Cho et al Nucleic Acids Res. 2018 May 18;46(9):e55
    Insert
    C7G697(Cas9 coding gene from Roseburia intestinalis L1-82)
    Use
    CRISPR; Cloning vector
    Tags
    Expression
    Mutation
    human codon-optimized
    Promoter
    Available since
    April 20, 2018
    Availability
    Academic Institutions and Nonprofits only
    103091-plasmid-map-sequence-id-201802

    Enlarge

  47. pUC19-D6GRK4

    Plasmid
    #103097
    Purpose
    Cas9 coding gene template with optimized sequence for human codon usage
    Depositor
    Duhee Bang
    Article
    Cho et al Nucleic Acids Res. 2018 May 18;46(9):e55
    Insert
    D6GRK4(Cas9 coding gene from Filifactor alocis (strain ATCC 35896 / D40 B5))
    Use
    CRISPR; Cloning vector
    Tags
    Expression
    Mutation
    human codon-optimized
    Promoter
    Available since
    April 20, 2018
    Availability
    Academic Institutions and Nonprofits only
    103097-plasmid-map-sequence-id-201807

    Enlarge

  48. pUC19-E3ELL7

    Plasmid
    #103099
    Purpose
    Cas9 coding gene template with optimized sequence for human codon usage
    Depositor
    Duhee Bang
    Article
    Cho et al Nucleic Acids Res. 2018 May 18;46(9):e55
    Insert
    E3ELL7(Cas9 coding gene from Bifidobacterium bifidum (strain S17))
    Use
    CRISPR; Cloning vector
    Tags
    Expression
    Mutation
    human codon-optimized
    Promoter
    Available since
    April 20, 2018
    Availability
    Academic Institutions and Nonprofits only
    103099-plasmid-map-sequence-id-201809

    Enlarge

  49. pUC19-E6WZS9

    Plasmid
    #103103
    Purpose
    Cas9 coding gene template with optimized sequence for human codon usage
    Depositor
    Duhee Bang
    Article
    Cho et al Nucleic Acids Res. 2018 May 18;46(9):e55
    Insert
    E6WZS9(Cas9 coding gene from Nitratifractor salsuginis (strain DSM 16511 / JCM 12458 / E9I37-1))
    Use
    CRISPR; Cloning vector
    Tags
    Expression
    Mutation
    human codon-optimized
    Promoter
    Available since
    April 20, 2018
    Availability
    Academic Institutions and Nonprofits only
    103103-plasmid-map-sequence-id-201812

    Enlarge

  50. pUC19-E7MR72

    Plasmid
    #103105
    Purpose
    Cas9 coding gene template with optimized sequence for human codon usage
    Depositor
    Duhee Bang
    Article
    Cho et al Nucleic Acids Res. 2018 May 18;46(9):e55
    Insert
    E7MR72(Cas9 coding gene from Solobacterium moorei F0204)
    Use
    CRISPR; Cloning vector
    Tags
    Expression
    Mutation
    human codon-optimized
    Promoter
    Available since
    April 20, 2018
    Availability
    Academic Institutions and Nonprofits only
    103105-plasmid-map-sequence-id-201814

    Enlarge

Showing: 1051 - 1100 of 158806 results