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pBW808_pCAG-PV5-VloxP-loxN-F3-GFP-BGHpA

Plasmid

#87584

Purpose

PV5 for 3-input, 2-output Full Adder

Depositor

Wilson Wong

Article

Weinberg et al Nat Biotechnol. 2017 Mar 27. doi: 10.103

Insert

EGFP

Use

Cre/Lox and Synthetic Biology

Tags

Expression

Mammalian

Mutation

Promoter

Available since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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CD200Cd4d3+4-blac

Plasmid

#32405

Purpose

A positive control receptor bait (rat CD4d3+4-blac) and prey (rat CD200) EXPRESs plasmid for The Basic AVEXIS kit

Depositor

Gavin Wright

Article

Bushell et al Genome Res. 2008 Apr;18(4):622-30. Epub

Insert

CD200

Use

Tags

Cd4d3+4 and beta-lactamase

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Jan. 17, 2012

Availability

Academic Institutions and Nonprofits only

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pH6HTC_PRDX6-Halo

Plasmid

#175338

Purpose

Production of HaloTag fusion protein

Depositor

Claudia Kutter

Article

Atanasoai et al bioRxiv 2021.11.08.467743

Insert

PRDX6 (PRDX6 Human)

Use

Cell free expression

Tags

HaloTag-6xHis

Expression

Bacterial

Mutation

Promoter

Available since

Oct. 20, 2021

Availability

Academic Institutions and Nonprofits only

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pBABE-puro-NLS-LSSmKate1

Plasmid

#31873

Depositor

Vladislav Verkhusha

Article

Piatkevich et al Proc Natl Acad Sci U S A. 2010 Mar 23;10

Insert

NLS-LSS-mKate1

Use

Retroviral

Tags

Expression

Mammalian

Mutation

K69Y/P131T/S148G/M167E/T183S/M196V compared to mK…

Promoter

Available since

Dec. 7, 2011

Availability

Academic Institutions and Nonprofits only

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pJEP212-Lenti-1.3CaMKII-P-Intron-MCS-WPRE-pA

Plasmid

#62931

Purpose

pLenti vector backbone containing a 1.3CaMKII promoter followed by an intron followed by multiple cloning site(MCS) (reverse orientation).

Depositor

Jonathan Ploski

Article

Holehonnur et al Mol Brain. 2015 Feb 24;8:12. doi: 10.118

Type

Empty backbone

Use

Lentiviral

Tags

Expression

Mutation

Promoter

1.3αCaMKII

Available since

March 14, 2022

Availability

Academic Institutions and Nonprofits only

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pSK ZO1

Plasmid

#30316

Depositor

Alan Fanning, James Anderson

Article

Willott et al Proc Natl Acad Sci U S A. 1993 Aug 15. 9

Insert

Zonula Occludens-1 (TJP1 Human)

Use

Subcloning

Tags

Expression

Mutation

Promoter

Available since

Oct. 6, 2011

Availability

Academic Institutions and Nonprofits only
Dppa1-T2A-H2B-Halo donor

Plasmid

#113118

Purpose

Donor plasmid for knock-in T2A-H2B-Halo to the c-terminal of mouse Dppa1 coding sequence

Depositor

Janet Rossant

Article

Gu et al Nat Biotechnol. 2018 Jun 11. pii: nbt.41

Insert

Dppa1 donor region including homology arms and T2A-H2B-Halo

Use

Synthetic Biology

Tags

Expression

Mutation

Promoter

Available since

Aug. 6, 2018

Availability

Academic Institutions and Nonprofits only

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pBW829_pCAG-pExpr-LUT

Plasmid

#87554

Purpose

Encodes Boolean Look-up Table logic in response to Cre, Flp, PhiC31, Vika, B3, and Bxb1 recombinases

Depositor

Wilson Wong

Article

Weinberg et al Nat Biotechnol. 2017 Mar 27. doi: 10.103

Insert

EGFP

Use

Cre/Lox and Synthetic Biology

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 7, 2017

Availability

Academic Institutions and Nonprofits only

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pNLS-LSSmKate1-N1

Plasmid

#31870

Depositor

Vladislav Verkhusha

Article

Piatkevich et al Proc Natl Acad Sci U S A. 2010 Mar 23;10

Insert

NLS

Use

Tags

Expression

Mammalian

Mutation

K69Y/P131T/S148G/M167E/T183S/M196V compared to mK…

Promoter

CMV

Available since

Sept. 9, 2011

Availability

Academic Institutions and Nonprofits only

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pTrichoGate-5

Plasmid

#160705

Purpose

Trichoderma optimised Synthetic eGFP bordered by BsaI restriction sites

Depositor

Artemio Mendoza

Article

Nogueira-Lopez et al Front Microbiol. 2019 Dec 10;10:2794. do

Insert

Trichoderma optimised Synthetic eGFP

Use

Synthetic Biology

Tags

Expression

Mutation

Promoter

Available since

Feb. 26, 2021

Availability

Academic Institutions and Nonprofits only

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pActinin-LSSmKate1-N1

Plasmid

#31906

Depositor

Vladislav Verkhusha

Article

Piatkevich et al Proc Natl Acad Sci U S A. 2010 Mar 23;10

Insert

alpha-actinin LSS-mKate1

Use

Tags

Expression

Mammalian

Mutation

K69Y/P131T/S148G/M167E/T183S/M196V compared to mK…

Promoter

Available since

Sept. 20, 2011

Availability

Academic Institutions and Nonprofits only

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cr3Dd

Plasmid

#184862

Purpose

cr3 helper plasmid for recombination and Cas9 induction; harbors the Cas9 endonuclease and a species-specific recombination system

Depositor

Patrick Sobetzko

Article

Teufel et al Nat Commun. 2022 Jun 14;13(1):3430. doi:

Insert

Cas9, bet, exo

Use

Tags

Cas9 ssrA degradation tag

Expression

Bacterial

Mutation

Promoter

Available since

Jan. 3, 2023

Availability

Academic Institutions and Nonprofits only

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SIN40C.SFFV.sfGFP-RepEntry.iPAC

Plasmid

#100893

Purpose

Lentiviral CRISPR-Cas9 Reporter

Depositor

Dirk Heckl

Article

Labuhn et al Nucleic Acids Res. 2018 Feb 16;46(3):137

Inserts

sfGFP
PAC

Use

Tags

Expression

Mutation

Promoter

Available since

Aug. 22, 2018

Availability

Academic Institutions and Nonprofits only

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pBW2287_pCAG-PV1-PYL-L1-FlpO-397C-NLS-BGHpA

Plasmid

#87560

Purpose

Expresses aa397-423 of Flp recombinase fused to PYL CID

Depositor

Wilson Wong

Article

Weinberg et al Nat Biotechnol. 2017 Mar 27. doi: 10.103

Insert

FlpO aa397-423

Use

Cre/Lox and Synthetic Biology

Tags

PYL

Expression

Mammalian

Mutation

Promoter

Available since

April 28, 2017

Availability

Academic Institutions and Nonprofits only

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pBW2286_pCAG-PV1-FlpO-N396-L1-ABI-NLS-BGHpA

Plasmid

#87559

Purpose

Expresses aa1-396 of Flp recombinase fused to ABI CID

Depositor

Wilson Wong

Article

Weinberg et al Nat Biotechnol. 2017 Mar 27. doi: 10.103

Insert

FlpO aa1-396

Use

Cre/Lox and Synthetic Biology

Tags

ABI

Expression

Mammalian

Mutation

Promoter

Available since

April 26, 2017

Availability

Academic Institutions and Nonprofits only

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pNLS-LSSmKate2-N1

Plasmid

#31871

Depositor

Vladislav Verkhusha

Article

Piatkevich et al Proc Natl Acad Sci U S A. 2010 Mar 23;10

Insert

NLS

Use

Tags

Expression

Mammalian

Mutation

K69Y/P131T/S148G/M167D/T183S/M196V compared to mK…

Promoter

Available since

Sept. 9, 2011

Availability

Academic Institutions and Nonprofits only

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CAV1-D67G-HA

Plasmid

#27706

Depositor

Ari Helenius

Article

Hayer et al Traffic. 2010 Mar . 11(3):361-82.

Insert

caveolin-1, mutant D67G (CAV1 Canis lupus familiaris)

Use

Tags

HA

Expression

Mammalian

Mutation

mutant D67G

Promoter

Available since

July 28, 2011

Availability

Academic Institutions and Nonprofits only

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plIVMD164

Plasmid

#160936

Purpose

In vivo mRNA display construct for GFP

Depositor

Saeed Tavazoie

Article

Oikonomou et al Proc Natl Acad Sci U S A. 2020 Oct 9. pi

Insert

MCP-sfGFP-HIS tag, 3'UTR MS2 Stem Loop

Use

Tags

HIS tag, 3'UTR MS2 Stem Loop

Expression

Yeast

Mutation

Promoter

MET25

Available since

Jan. 7, 2021

Availability

Academic Institutions and Nonprofits only

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pCKTRBS-OD

Plasmid

#102924

Purpose

pCK01 derivative carrying tetR under a constitutive promoter and an aTc-inducible PtetO promoter for control expression of genes. Contains LacI OD for translational fusions on the Ct of cloned genes.

Depositor

George Church

Article

Juarez et al Nat Commun. 2018 Aug 6;9(1):3101. doi: 1

Type

Empty backbone

Use

Tags

Expression

Bacterial

Mutation

Promoter

PtetO

Available since

Jan. 23, 2019

Availability

Academic Institutions and Nonprofits only

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pH6HTC_IGF2BP2b-Halo

Plasmid

#175318

Purpose

Production of HaloTag fusion protein

Depositor

Claudia Kutter

Article

Atanasoai et al bioRxiv 2021.11.08.467743

Insert

IGF2BP2 (IGF2BP2 Human)

Use

Cell free expression

Tags

HaloTag-6xHis

Expression

Bacterial

Mutation

Isoform B

Promoter

Available since

Oct. 18, 2021

Availability

Academic Institutions and Nonprofits only

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