-
Plasmid#235584PurposeDox-inducible expression of control catalytic-mutant Ezh2 fused with scFVDepositorInsertEzh2 (Ezh2 Mouse)
UseCRISPRTagsExpressionMutationY726DPromoterAvailable sinceApril 30, 2025AvailabilityAcademic Institutions and Nonprofits only -
pET28a-SENP2 (catalytic domain)
Plasmid#16357DepositorInserthuman SENP2 catalytic domain (SENP2 Human)
UseTagsHisExpressionBacterialMutationAmino Acid 365 to 590 catalytic domain of human S…PromoterAvailable sinceDec. 19, 2007AvailabilityAcademic Institutions and Nonprofits only -
pET28a-SENP7 (catalytic domain)
Plasmid#16360DepositorInserthuman SENP7 catalytic domain (SENP7 Human)
UseTagsHisExpressionBacterialMutationAmino Acid 640 to 984 catalytic domain of human S…PromoterAvailable sinceDec. 19, 2007AvailabilityAcademic Institutions and Nonprofits only -
human gamma-catenin pcDNA3
Plasmid#16827DepositorInsertgamma-catenin (JUP Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceApril 29, 2008AvailabilityAcademic Institutions and Nonprofits only -
EFS-SpdCas9-Dnmt3A/3L-catΔ
Plasmid#222501PurposeExpresses EFS promoter driven human codon optimized SpdCas9 directly fused to Dnmt3A/3L variant catΔ (C706A and R832E) followed by P2A-mCherryDepositorInsertS. pyogenes dCas9 with C-terminal fusion of murine Dnmt3A-Dnmt3L variant catΔ (C706A and R832E) engineered fusion
UseLentiviralTagsFLAG TagExpressionMammalianMutationD10A and H840A in S.pyogenes Cas9; C706A and R832…PromoterEFSAvailable sinceFeb. 7, 2025AvailabilityAcademic Institutions and Nonprofits only -
pET30a-His6-ABP-LOXCAT
Plasmid#139971PurposeFusion of A. viridans lactate oxidase (LOX) and E. coli catalase (CAT) that converts lactate into pyruvate without producing H2O2. Contains a albumin binding peptide (ABP).DepositorInsertA fusion of lactate oxidase from A. viridans and catalase from E. coli
UseTagsHis6ExpressionBacterialMutationPromoterT7Available sinceSept. 16, 2020AvailabilityAcademic Institutions and Nonprofits only -
alpha catenin conformation sensor
Plasmid#71709PurposeThis alpha catenin biosensor undergoes a change in FRET upon mechanical perturbation of the alpha catenin conformation.DepositorInsertalpha-catenin FRET Sensor
UseTagsECFP and YPETExpressionMammalianMutationPromoterCMVAvailable sinceApril 19, 2016AvailabilityAcademic Institutions and Nonprofits only -
pET28a-SENP1 (catalytic domain)
Plasmid#16356DepositorInserthuman SENP1 catalytic domain (SENP1 Human)
UseTagsHisExpressionBacterialMutationAmino Acid 419 to 644 catalytic domain of human S…PromoterAvailable sinceDec. 19, 2007AvailabilityAcademic Institutions and Nonprofits only -
beta-catenin (S33Y)-pcw107
Plasmid#64615Purposewhen used to produce lentivirus, express physiological levels of insertDepositorInsertCTNNB1 (CTNNB1 Human)
UseLentiviralTagsExpressionMutationS33YPromoterPGKAvailable sinceMay 12, 2015AvailabilityAcademic Institutions and Nonprofits only -
pEGFP-C1 beta-catenin Y654F
Plasmid#16072DepositorInsertbeta-catenin (CTNNB1 Chicken)
UseTagsEGFPExpressionMammalianMutationY654F, *(see below)PromoterAvailable sinceNov. 2, 2007AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3 deltaN47 Beta-catenin
Plasmid#19287DepositorInsertbeta-catenin (CTNNB1 Human)
UseTagsFlagExpressionMammalianMutationN-terminal truncation, deleted aa 1-47PromoterAvailable sinceSept. 8, 2008AvailabilityAcademic Institutions and Nonprofits only -
BcatMutS33/S37.T41/S45
Plasmid#24204DepositorInsertbeta catenin (CTNNB1 Human)
UseTagsFLAGExpressionMammalianMutationS33A, S37A, T41A,S45APromoterCMVAvailable sinceMarch 9, 2010AvailabilityAcademic Institutions and Nonprofits only -
beta-catenin (S33Y)-pcw107-V5
Plasmid#64616Purposewhen used to produce lentivirus, express physiological levels of insertDepositorInsertCTNNB1 (CTNNB1 Human)
UseLentiviralTagsV5ExpressionMutationS33YPromoterPGKAvailable sinceMay 12, 2015AvailabilityAcademic Institutions and Nonprofits only -
pMXs-beta-catenin-S33Y
Plasmid#13371PurposeRetroviral expression of mouse beta-catenin S33Y mutantDepositorInsertcatenin (cadherin associated protein), beta 1 (S33Y mutant) (Ctnnb1 Mouse)
UseRetroviralTagsExpressionMammalianMutationSerine 33 to Tyrosine and Leucine 388 to ProlinePromoterAvailable sinceJan. 12, 2007AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-HA-PHLPP1 N-terminal truncation
Plasmid#22404PurposeExpresses a HA tagged, N-terminal truncation of human PHLPP1 in mammalian cellsDepositorInsertPHLPP1 (PHLPP1 Human)
UseTagsHAExpressionMammalianMutationPromoterAvailable sinceJan. 29, 2010AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3 deltaN89 Beta-catenin
Plasmid#19288DepositorInsertbeta-catenin (CTNNB1 Human)
UseTagsFlagExpressionMammalianMutationN-terminal truncation, deleted aa 1-89PromoterAvailable sinceSept. 8, 2008AvailabilityAcademic Institutions and Nonprofits only -
CMV-NanoLuc-BRAF-CAT
Plasmid#194772PurposeMammalian expression construct encoding the BRAF catalytic (CAT) domain (AA 435-766) with an N-terminal NanoLuc tag.DepositorInsertBRAF catalytic (CAT) domain (AA 435-766) (BRAF Human)
UseTagsNanoLucExpressionMammalianMutationPromoterCMV51Available sinceJune 20, 2023AvailabilityAcademic Institutions and Nonprofits only -
CaTCH Dual-sgRNA_sgBC1-A_sgBC1-C
Plasmid#154196PurposeLentiviral expression plasmid encoding two sgRNAs for targeting of the CaTCH barcode cassette BC1. Expresses Thy1.1 and a Neo selection marker.DepositorInsertsgRNA-BC1-A, sgRNA-BC1-C
UseLentiviral; Catch barcode activationTagsExpressionMammalianMutationPromoterhU6 and mU6Available sinceDec. 2, 2020AvailabilityAcademic Institutions and Nonprofits only -
pET28_6xHis-PARP1-CAT-L713F
Plasmid#173944PurposeBacterial expression of isolated PARP1 CAT domain containing L713F gain-of-function mutationDepositorInsertPARP1-CAT-L713F (PARP1 Human)
UseTags6xHisExpressionBacterialMutationL713F, V762APromoterAvailable sinceSept. 30, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCDF-His-mTET1CD∆cat
Plasmid#81054Purposebacterial expression of catalytic dead catalytic domain of murine TET1DepositorInsertTET1 (Tet1 Mouse)
UseTags6HISExpressionBacterialMutationcatalytic domain amino acid 1367-2057 with histid…PromoterT7Available sinceAug. 19, 2016AvailabilityAcademic Institutions and Nonprofits only