4,182 results

1. pCMV-T7-SpRY-HF1-P2A-EGFP (RTW5008)

   Plasmid

   #139997

   Purpose

   CMV and T7 promoter expression plasmid for human codon optimized SpRY-HF1(SpRY=A61R/L1111R/D1135L/S1136W/G1218K/E1219Q/N1317R/A1322R/R1333P/R1335Q/T1337R;HF1=N497A/R661A/Q695A/Q926A) with a c-terminal bi-partite NLS, 3x flag tag, and P2A-EGFP

   Depositor

   Benjamin Kleinstiver

   Article

   Walton et al Science. 2020 Apr 17;368(6488):290-296.

   Insert

   human codon optimized SpCas9 variant named SpRY-HF1 with BPNLS-3xFLAG-P2A-EGFP
   

   Use

   CRISPR; In vitro transcription; t7 promoter

   Tags

   BPNLS-3xFLAG-P2A-EGFP

   Expression

   Mammalian

   Mutation

   SpRY=A61R/L1111R/D1135L/S1136W/G1218K/E1219Q/N131…

   Promoter

   CMV and T7

   Available Since

   March 26, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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2. pKMyc

   Plasmid

   #19400

   Depositor

   Ian Macara

   Article

   Joberty et al Nat Cell Biol 2000 Aug;2(8):531-9.

   Type

   Empty backbone

   Tags

   Myc

   Expression

   Mammalian

   Available Since

   April 2, 2009

   Availability

   Academic Institutions and Nonprofits only

   

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3. pcDNA3.3_SOX2

   Plasmid

   #26817

   Depositor

   Derrick Rossi

   Article

   Warren et al Cell Stem Cell. 2010 Nov 5;7(5):618-30.

   Insert

   5'UTR-SOX2-3'UTR (SOX2 Human)
   

   Expression

   Mammalian

   Available Since

   Nov. 17, 2010

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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4. pcDNA3-ClopHensor

   Plasmid

   #25938

   Depositor

   Daniele Arosio

   Article

   Arosio et al Nat Methods. 2010 Jul . 7(7):516-8.

   Insert

   ClopHensor
   

   Tags

   Strep-II

   Expression

   Mammalian

   Mutation

   E2GFP is EGFP-T203Y

   Available Since

   Sept. 13, 2010

   Availability

   Academic Institutions and Nonprofits only

   

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5. pDY0663 ACTB atgRNA with v2 scaffold

   Plasmid

   #179108

   Purpose

   ACTB N-term PBS 13 RT 29 Bxb1 AttB 46 atgRNA with v2 scaffold

   Depositor

   Omar Abudayyeh, Jonathan Gootenberg

   Article

   Yarnall et al Nat Biotechnol. 2022 Nov 24. doi: 10.103

   Insert

   ACTB N-term PBS 13 RT 29 AttB 46 atgRNA with v2 scaffold
   

   Use

   CRISPR

   Available Since

   Feb. 17, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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6. pAAV-hSyn-hChR2(H134R)-EYFP

   Plasmid

   #26973

   Purpose

   AAV expression of humanized ChR2 with H134R mutation fused to EYFP driven by human Synapsin I promoter for optogenetic excitation

   Depositor

   Karl Deisseroth

   Has Service

   AAV PHP.eB, AAV Retrograde, AAV1, AAV2, AAV5, and AAV9

   Insert

   hChR2(H134R)
   

   Use

   AAV

   Tags

   EYFP

   Expression

   Mammalian

   Mutation

   H134R

   Promoter

   hSyn

   Available Since

   Feb. 4, 2011

   Availability

   Academic Institutions and Nonprofits only

   

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7. pDEST-HisMBP

   Plasmid

   #11085

   Depositor

   David Waugh

   Article

   Nallamsetty et al Protein Sci. 2005 Dec . 14(12):2964-71.

   Has Service

   Cloning Grade DNA

   Type

   Empty backbone

   Tags

   6xHis and MBP

   Expression

   Bacterial

   Promoter

   Tac (lactose/IPTG inducible)

   Available Since

   Feb. 14, 2006

   Availability

   Academic Institutions and Nonprofits only

   

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8. pcDNA3.1 MCS-BirA(R118G)-HA

   Plasmid

   #36047

   Purpose

   To fuse your protein of interest to the N-terminus of BirA(R118G) and use in proximity-dependent biotin identification (BioID); HA tag

   Depositor

   Kyle Roux

   Article

   Roux et al J Cell Biol. 2012 Mar 12.

   Has Service

   Cloning Grade DNA

   Insert

   BirA(R118G)-HA
   

   Tags

   HA

   Expression

   Mammalian

   Mutation

   R118G (highly promiscuous form)

   Available Since

   April 13, 2012

   Availability

   Academic Institutions and Nonprofits only

   

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9. pGRG36

   Plasmid

   #16666

   Depositor

   Nancy Craig

   Article

   McKenzie et al BMC Microbiol. 2006 Apr 28;6:39. doi: 10

   Insert

   mTn7::MCS
   

   Expression

   Bacterial

   Available Since

   Jan. 10, 2008

   Availability

   Academic Institutions and Nonprofits only

   

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10. HA-NFAT1(4-460)-GFP

    Plasmid

    #11107

    Purpose

    Expresses mouse NFAT1 (amino acids 4-460) with HA and GFP tags in mammalian cells

    Depositor

    Anjana Rao

    Article

    Aramburu et al Science. 1999 Sep 24. 285(5436):2129-33.

    Insert

    NFAT1 (Nfatc2 Mouse)
    

    Tags

    3x HA and GFP

    Expression

    Mammalian

    Mutation

    Contains amino acids 4-460 of NFAT1; L78P compare…

    Promoter

    CMV

    Available Since

    May 31, 2006

    Availability

    Academic Institutions and Nonprofits only

    

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