19,640 results

1. pHR_Gal4UAS_humanIL12flexi _IRES_mCherry_PGK_tBFP

   Plasmid

   #85428

   Purpose

   Lentiviral vector for Gal4 inducible humanIL-12flexi with mCherry reporter and constitutive tBFP expression

   Depositor

   Wendell Lim

   Article

   Roybal et al Cell. 2016 Oct 6;167(2):419-432.e16. doi

   Insert

   human IL-12
   

   Use

   Lentiviral

   Available Since

   Aug. 17, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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2. pOTTC407 - pAAV EF1a eGFP

   Plasmid

   #60058

   Purpose

   An AAV packaging vector that expresses enhanced green fluorescent protein under control of the EF1a promoter.

   Depositor

   Brandon Harvey

   Article

   Savolainen et al Neurobiol Dis. 2014 Aug;68:1-15. doi: 10

   Insert

   enhanced Green Fluorescent Protein
   

   Use

   AAV

   Expression

   Mammalian

   Promoter

   EF1a

   Available Since

   Oct. 9, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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3. pLVX-RT003-GPM

   Plasmid

   #163383

   Purpose

   To express sGFP module in the G-baToN system

   Depositor

   Monte Winslow

   Article

   Tang et al Elife. 2020 Oct 7;9. pii: 61080. doi: 10

   Insert

   GFP-PDGFRtm-2A-mCherry (GPM)
   

   Use

   Lentiviral

   Tags

   mCherry

   Expression

   Bacterial and Mammalian

   Promoter

   EF1a

   Available Since

   Jan. 11, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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4. PB533-42B3mutAC2-sfGFP

   Plasmid

   #186777

   Purpose

   RNAP2 Ser2ph-mintbody [42B3(R78K/A80T/M95V)-scFv] expression vector for mammalian cells

   Depositor

   Hiroshi Kimura

   Article

   Uchino et al J Cell Biol. 2022 Feb 7;221(2). pii: 212

   Insert

   42B3mutAC2-scFv
   

   Tags

   sfGFP

   Expression

   Mammalian

   Available Since

   Aug. 9, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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5. p414-TEF1p-Cas9-CYC1t

   Plasmid

   #43802

   Purpose

   A human codon-optimized Cas9 for expression in S. cerevisiae (budding yeast) from the TEF1 promoter

   Depositor

   George Church

   Article

   Dicarlo et al Nucleic Acids Res

   Insert

   Human Optimized S. pyogenes Cas9
   

   Use

   CRISPR

   Expression

   Yeast

   Promoter

   TEF1 promoter

   Available Since

   March 15, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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6. pLenti-HRE-dUnaG

   Plasmid

   #124372

   Purpose

   UnaG fluorescent protein reporter for hypoxia-induced factor (HIF) signaling

   Depositor

   Roland Friedel

   Article

   Sattiraju et al Immunity. 2023 Jul 7:S1074-7613(23)00274

   Insert

   HRE-dUnaG
   

   Use

   Lentiviral

   Tags

   PEST-degron and Myc tag

   Promoter

   HRE (HIF responsive element 5x + CMV minimal prom…

   Available Since

   July 20, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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7. pmScarlet_C1

   Plasmid

   #85042

   Purpose

   Mammalian expression of mScarlet

   Depositor

   Dorus Gadella

   Article

   Bindels et al Nat Methods. 2016 Nov 21. doi: 10.1038/n

   Has Service

   Cloning Grade DNA

   Insert

   mScarlet
   

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Dec. 6, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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8. lenti U6-sgRNA/EF1a-mCherry

   Plasmid

   #114199

   Purpose

   Lentivirus compatible SpCas9/dCas9 sgRNA scaffold driven by the U6 promoter and mCherry driven by the EF1a promoter

   Depositor

   Jeremy Day

   Article

   Savell et al eNeuro. 2019 Mar 7;6(1). pii: eN-MNT-049

   Insert

   sgRNA scaffold
   

   Use

   Lentiviral

   Tags

   mCherry

   Expression

   Mammalian

   Promoter

   U6

   Available Since

   Sept. 7, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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9. TRE reporter backbone

   Plasmid

   #158678

   Purpose

   This plasmid serves as the backbone for the TRE reporters described in our paper. It's a promoterless-mStrawberry-pGK-BSD backbone to which we insert a pathway specific promoter before the mStrawberry

   Depositor

   Ravid Straussman

   Article

   Kaufman et al Nat Commun. 2022 May 18;13(1):2725. doi:

   Type

   Empty backbone

   Use

   Lentiviral

   Tags

   mStrawberry

   Expression

   Mammalian

   Available Since

   May 18, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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10. 3xHA-miniTurbo_pAS32

    Plasmid

    #118222

    Purpose

    expresses 3xHA-tagged miniTurbo in C. elegans intestine

    Depositor

    Jessica Feldman

    Article

    Branon et al Nat Biotechnol. 2018 Aug 20. pii: nbt.42

    Insert

    miniTurbo (BirA mutant)
    

    Tags

    3x HA tag

    Expression

    Worm

    Mutation

    aa1-63 deleted; Q65P, I87V, R118S, E140K, Q141R, …

    Promoter

    ges-1p

    Available Since

    Jan. 14, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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11. pCLX-PGK-hTERT

    Plasmid

    #114315

    Purpose

    Constitutive expression of the hTert protein

    Depositor

    Patrick Salmon

    Insert

    hTERT (TERT Human)
    

    Use

    Lentiviral

    Available Since

    Sept. 11, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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12. pEGFP-N1 alpha-actinin 1

    Plasmid

    #11908

    Depositor

    Carol Otey

    Article

    Edlund et al Cell Motil Cytoskeleton. 2001 Mar . 48(3

    Insert

    alpha-actinin 1 (ACTN1 Human)
    

    Tags

    eGFP

    Expression

    Mammalian

    Available Since

    Aug. 7, 2006

    Availability

    Academic Institutions and Nonprofits only

    

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13. pGFP Cortactin

    Plasmid

    #50728

    Purpose

    mammalian expression of GFP-Cortactin. Note- This plasmid contains GFP21, which has a sequence similar to YFP, but emission/excitation similar to GFP.

    Depositor

    Kenneth Yamada

    Insert

    Cortactin (CTTN Human)
    

    Tags

    GFP

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Jan. 7, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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14. pLKO-1 shRev-Erbalpha

    Plasmid

    #22747

    Depositor

    Bruce Spiegelman

    Article

    Estall et al Proc Natl Acad Sci U S A. 2009 Dec 29. 1

    Insert

    shRev-Erbalpha (Nr1d1 Mouse)
    

    Use

    Lentiviral and RNAi

    Expression

    Mammalian

    Available Since

    Feb. 8, 2010

    Availability

    Academic Institutions and Nonprofits only

15. KRAB-dCas9

    Plasmid

    #112195

    Purpose

    KRAB fused to N-terminus of dCas9; pCDNA3 vector backbone, mammalian expression

    Depositor

    David Segal

    Article

    O'Geen et al Nucleic Acids Res. 2017 Sep 29;45(17):99

    Insert

    KRAB (ZNF10 Human)
    

    Use

    CRISPR

    Tags

    3XFLag-NLS-KRAB-dCas9-NLS

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    July 18, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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16. pCAGGs-NLS-TIR1_P2A_NES-TIR1

    Plasmid

    #117699

    Purpose

    Expression of NLS-(Os)TIR1-HA and Myc-NES-(Os)TIR1from one mRNA

    Depositor

    Joerg Mansfeld

    Article

    Daniel et al Nat Commun. 2018 Aug 17;9(1):3297. doi:

    Inserts

    TRANSPORT INHIBITOR RESPONSE 1
    TRANSPORT INHIBITOR RESPONSE 1
    

    Tags

    HA tag, Myc-NES, and NLS

    Expression

    Mammalian

    Mutation

    codon optimized for murine expression

    Promoter

    None, 2A peptide from porcine teschovirus-1 polyp…

    Available Since

    Jan. 18, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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17. pAAVS1-P-CAG-DEST

    Plasmid

    #80490

    Purpose

    Gateway Destination donor vector for AAVS1 targeting (puromycin selection)

    Depositor

    Knut Woltjen

    Article

    Oceguera-Yanez et al Methods. 2015 Dec 18. pii: S1046-2023(15

    Type

    Empty backbone

    Use

    Donor vector (human)

    Expression

    Mammalian

    Available Since

    Nov. 28, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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18. Flag-hPIASx beta

    Plasmid

    #15210

    Depositor

    Ke Shuai

    Article

    Arora et al J Biol Chem. 2003 Jun 13. 278(24):21327-

    Insert

    PIASx beta (PIAS2 Human)
    

    Tags

    Flag

    Expression

    Mammalian

    Available Since

    Feb. 8, 2008

    Availability

    Academic Institutions and Nonprofits only

    

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19. c-Myc-5-HT2A

    Plasmid

    #67944

    Purpose

    N-terminally c-myc tagged human 5-hydroxytryptamine receptor 2A

    Depositor

    Javier Gonzalez-Maeso

    Article

    Gonzalez-Maeso et al Nature. 2008 Mar 6;452(7183):93-7. doi:

    Insert

    HTR2A (HTR2A Human)
    

    Tags

    c-Myc

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Sept. 24, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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20. lentiCRISPR v2-sgSLC7A11/xCT-1

    Plasmid

    #161818

    Purpose

    knock out SLC7A11/xCT in mammalian cells

    Depositor

    Boyi Gan

    Article

    Liu et al Nat Cell Biol. 2020 Apr;22(4):476-486. d

    Insert

    SLC7A11 solute carrier family 7 member 11 xCT (SLC7A11 Human)
    

    Use

    CRISPR and Lentiviral

    Available Since

    Dec. 8, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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