We narrowed to 9,820 results for: 158

1. pShoHELIX-sgRNA_entry (BO3)

   Plasmid

   #181784

   Purpose

   Expresses ShoHELIX containing a nicking I-AniI fusion to ShoTnsB. New sgRNA spacer sequences can be added using Golden Gate assembly (via SapI sites).

   Depositor

   Benjamin Kleinstiver

   Article

   Tou et al Nat Biotechnol. 2023 Jan 2. doi: 10.1038

   Insert

   nAniI-ShoTnsB, ShoTnsC, ShoTniQ, ShoCas12k
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   nAniI = K227M, F80K, L232K

   Promoter

   Lac and J23119

   Available since

   March 31, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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2. shCDK4/6(1)

   Plasmid

   #73554

   Purpose

   vector encoding both shRNA against CDK4(1) and shRNA against CDK6(1)

   Depositor

   Gerardo Ferbeyre

   Article

   Acevedo et al Cancer Res. 2016 Jun 1;76(11):3252-64. d

   Insert

   shRNA against CDK4 + shRNA against CDK6
   

   Use

   Retroviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   LTR

   Available since

   Aug. 8, 2016

   Availability

   Academic Institutions and Nonprofits only

3. pCR1446

   Plasmid

   #111129

   Purpose

   Expresses FANCF

   Depositor

   Jacob Corn

   Article

   Richardson et al Nat Genet. 2018 Aug;50(8):1132-1139. doi

   Insert

   FANCF (FANCF Human)
   

   Use

   Lentiviral

   Tags

   HA

   Expression

   Mutation

   Promoter

   Available since

   Oct. 18, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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4. pXWJ109As(RS-te-AAV)

   Plasmid

   #123156

   Purpose

   For TEV-based leakiness regulation. pSB3K3 carrying J109-30arsR-t-ParsR-30hrpR-30hrpS-t-PhrpLE-30gfp-te-AAV-t

   Depositor

   Baojun Wang

   Article

   Wan et al Nat Chem Biol. 2019 May;15(5):540-548. d

   Insert

   J109-30arsR-t-ParsR-30hrpR-30hrpS-t-PhrpLE-30gfp-te-AAV-t
   

   Use

   Synthetic Biology

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   J109, ParsR, PhrpLE

   Available since

   May 9, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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5. pLV-EF1a-DHB-mTurq2

   Plasmid

   #212667

   Purpose

   Express tagged DHB

   Depositor

   Tobias Meyer, Yumi Konagaya

   Article

   Konagaya et al Nature. 2024 Jul;631(8020):424-431. doi:

   Insert

   DHB (HELB Human)
   

   Use

   Lentiviral

   Tags

   mTurq2

   Expression

   Mutation

   Promoter

   EF-1a

   Available since

   Aug. 5, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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6. pCMV-BE4-SsAPOBEC3B R54Q

   Plasmid

   #138344

   Purpose

   Mammalian expression plasmid for BE4-SsAPOBEC3B R54Q

   Depositor

   Nicole Gaudelli

   Article

   Yu et al Nat Commun. 2020 Apr 28;11(1):2052. doi:

   Insert

   BE4-SsAPOBEC3B R54Q
   

   Use

   Synthetic Biology

   Tags

   BP-NLS

   Expression

   Mutation

   Promoter

   CMV

   Available since

   April 28, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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7. shCDK4/6(2)

   Plasmid

   #73555

   Purpose

   vector encoding both shRNA against CDK4(2) and shRNA against CDK6(2)

   Depositor

   Gerardo Ferbeyre

   Article

   Acevedo et al Cancer Res. 2016 Jun 1;76(11):3252-64. d

   Insert

   shRNA against CDK4 + shRNA against CDK6
   

   Use

   Retroviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   LTR

   Available since

   Aug. 8, 2016

   Availability

   Academic Institutions and Nonprofits only

8. U6-3' MS2-CircRNA

   Plasmid

   #213751

   Purpose

   For circular RNA-mediated prime editor using U6-3' MS2-CircRNA in HEK293T cells

   Depositor

   Caixia Gao

   Article

   Liang et al Nat Biotechnol. 2024 Jan 10. doi: 10.103

   Insert

   5' ribozyme, 5' ligation sequences, 3' MS2, 3' ligation sequences, 3' ribozyme
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available since

   Feb. 7, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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9. pHR- flag-Lag6-iCAM-1

   Plasmid

   #205210

   Purpose

   This vector encodes of the synCAM (iCAM1) and GFP nanobody LAG6

   Depositor

   Wendell Lim

   Article

   Stevens et al Nature. 2023 Feb;614(7946):144-152. doi:

   Insert

   synCAM iCAM1 and Lag6 anti-GFP nanobody
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Aug. 22, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pHR- flag-Lag18-iCAM-1

    Plasmid

    #205207

    Purpose

    This vector encodes of the synCAM (iCAM1) and GFP nanobody LAG18

    Depositor

    Wendell Lim

    Article

    Stevens et al Nature. 2023 Feb;614(7946):144-152. doi:

    Insert

    synCAM iCAM1 and Lag18 anti-GFP nanobody
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 22, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pHR- flag-Lag42-iCAM-1

    Plasmid

    #205205

    Purpose

    This vector encodes of the synCAM (iCAM1) and GFP nanobody LAG42

    Depositor

    Wendell Lim

    Article

    Stevens et al Nature. 2023 Feb;614(7946):144-152. doi:

    Insert

    synCAM iCAM1 and Lag42 anti-GFP nanobody
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 22, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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12. pcDNA5_NLS-Halo-eGFP-BioID

    Plasmid

    #218448

    Purpose

    expresses the NLS-Halo-eGFP-BioID fusion protein via stable Flp-In integration

    Depositor

    Thorsten Stafforst

    Article

    Hanswillemenke et al Nat Chem Biol. 2024 Jan 17. doi: 10.1038

    Insert

    NLS-Halo-eGFP-BirA
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    June 10, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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13. pEB2-mNeptune2.5

    Plasmid

    #104013

    Purpose

    Plasmid encoding mNeptune2.5

    Depositor

    Philippe Cluzel

    Article

    Balleza et al Nat Methods. 2018 Jan;15(1):47-51. doi:

    Insert

    mNeptune2.5
    

    Use

    Low copy

    Tags

    Expression

    Bacterial

    Mutation

    Mutations relative to eqFP578 (MSKGEE LIKENM… M11…

    Promoter

    proC

    Available since

    Dec. 28, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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14. pSL2621 (pCas8_Vch)

    Plasmid

    #200893

    Purpose

    Expresses human codon-optimized V. cholerae Cas8 from a CMV promoter.

    Depositor

    Samuel H. Sternberg

    Article

    Lampe et al Nat Biotechnol. 2023 Mar 29. doi: 10.103

    Insert

    VchCas8
    

    Use

    Tags

    NLS

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    May 23, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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15. pSL2623 (pCas6_Vch)

    Plasmid

    #200895

    Purpose

    Expresses human codon-optimized V. cholerae Cas6 from a CMV promoter.

    Depositor

    Samuel H. Sternberg

    Article

    Lampe et al Nat Biotechnol. 2023 Mar 29. doi: 10.103

    Insert

    VchCas6
    

    Use

    Tags

    NLS

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    May 22, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. mEos3.2trCD45

    Plasmid

    #203753

    Purpose

    Encodes the transmembrane domain from CD45 with mEos3.2 fused on the N terminus. Used as a fluorescent plasma membrane probe.

    Depositor

    Sarah Veatch

    Article

    Shelby et al Nat Chem Biol. 2023 Jun;19(6):750-758. d

    Insert

    trCD45 (PTPRC Human)
    

    Use

    Tags

    mEos3.2

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 7, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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17. MMLV-RT for Split-PE (pEH45)

    Plasmid

    #190105

    Purpose

    Expresses MMLV-RT (full-length, from PE2) for Split-PE use

    Depositor

    Keith Joung

    Article

    Grunewald et al Nat Biotechnol. 2023 Mar;41(3):337-343.

    Insert

    MMLV-RT
    

    Use

    Tags

    bpNLS

    Expression

    Mammalian

    Mutation

    RT pentamutant from PE2 (D200N, T306K, W313F, T33…

    Promoter

    CMV

    Available since

    Sept. 26, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pET28a_Pro-IL-36γ

    Plasmid

    #214345

    Purpose

    Protein overexpression in bacteria

    Depositor

    Jonathan Kagan

    Article

    Devant et al Nature. 2023 Dec;624(7991):451-459. doi:

    Insert

    IL36G (IL36G Human)
    

    Use

    Tags

    His-TEV and Myc

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Feb. 21, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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19. p2T-CAG-MCS-P2A-GFP-PuroR

    Plasmid

    #107186

    Purpose

    Base plasmid for cloning gene duplication alleles with eGFP reporter.

    Depositor

    Richard Sherwood

    Article

    Shen et al Nature. 2018 Nov;563(7733):646-651. doi:

    Type

    Empty backbone

    Use

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Dec. 6, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pCW-CCND1-HA-puro

    Plasmid

    #212670

    Purpose

    Express tagged cyclin D1

    Depositor

    Tobias Meyer, Yumi Konagaya

    Article

    Konagaya et al Nature. 2024 Jul;631(8020):424-431. doi:

    Insert

    CCND1 (CCND1 Human)
    

    Use

    Lentiviral

    Tags

    HA

    Expression

    Mutation

    Promoter

    TRE

    Available since

    Aug. 5, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pSL2622 (pCas7_Vch)

    Plasmid

    #200894

    Purpose

    Expresses human codon-optimized V. cholerae Cas7 from a CMV promoter.

    Depositor

    Samuel H. Sternberg

    Article

    Lampe et al Nat Biotechnol. 2023 Mar 29. doi: 10.103

    Insert

    VchCas7
    

    Use

    Tags

    NLS

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 26, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. nTRb412-gs10-NLS-VPR

    Plasmid

    #188267

    Purpose

    Plasmid ensures constitutive expression of the N-terminal fragment of split TRbeta (aa 200-412), fused to the VPR activation domain, a flexible GS linker and the SV40 NLS signal at the C-terminus

    Depositor

    Roman Jerala

    Article

    Rihtar et al Nat Chem Biol. 2022 Sep 26. doi: 10.1038

    Insert

    nTRb412 (TXNRD2 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 17, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pRRL-5xTetO-pEF-H2B-Citrine-SV40

    Plasmid

    #186966

    Purpose

    Lentiviral vector encoding fluorescent reporter (5xTetO-pEF-H2B-Citrine-SV40) for expression in mammalian cells.

    Depositor

    Brian Liau

    Article

    Lue et al Nat Chem Biol. 2023 Feb;19(2):176-186. d

    Insert

    5xTetO-pEF-H2B-Citrine-SV40
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    5xTetO-pEF-1alpha

    Available since

    Oct. 14, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pBR322-Pdp1_NTD-ZFD_R

    Plasmid

    #198277

    Purpose

    For loading R half of zinc finger deaminase targeting human TRAC locus (ZFD_TRAC-NC-Right-G1397-C_DddA); also contains PVC regulatory genes

    Depositor

    Feng Zhang

    Article

    Kreitz et al Nature. 2023 Apr;616(7956):357-364. doi:

    Insert

    Pdp1_NTD-ZFD_R
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    March 29, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pCMV-BE4-PpAPOBEC1

    Plasmid

    #138349

    Purpose

    Mammalian expression plasmid for BE4-PpABOBEC1

    Depositor

    Nicole Gaudelli

    Article

    Yu et al Nat Commun. 2020 Apr 28;11(1):2052. doi:

    Insert

    BE4-PpAPOBEC1
    

    Use

    Tags

    BP-NLS

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    April 28, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pJB556

    Plasmid

    #115966

    Purpose

    SGP insert for replicon MoClo

    Depositor

    Ron Weiss

    Article

    Wagner et al Nat Chem Biol. 2018 Nov;14(11):1043-1050

    Insert

    Lvl0 -98/15 SGP 2xTetApt-STOP
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Dec. 14, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pMCSG28-SH3-588

    Plasmid

    #185920

    Purpose

    Expresses SH3-588, a designed poly-proline peptide recruiting catechol O-methyltransferase

    Depositor

    Brian Kuhlman

    Article

    Park et al Nat Chem Biol. 2023 Apr;19(4):460-467. d

    Insert

    SH3-588
    

    Use

    Tags

    6x HIS tag

    Expression

    Bacterial

    Mutation

    Designed enzyme with novel interface between huma…

    Promoter

    T7

    Available since

    Dec. 12, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pCMV-BE4-PpAPOBEC1 H122A

    Plasmid

    #138338

    Purpose

    Mammalian expression plasmid for BE4-PpABOBEC1H122A

    Depositor

    Nicole Gaudelli

    Article

    Yu et al Nat Commun. 2020 Apr 28;11(1):2052. doi:

    Insert

    BE4-PpAPOBEC1 H122A
    

    Use

    Synthetic Biology

    Tags

    BP-NLS

    Expression

    Mutation

    Change of His122 to Ala.

    Promoter

    CMV

    Available since

    April 28, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. polyPRM5

    Plasmid

    #175232

    Purpose

    Bacterial expression and purification, mixed with polySH3 to form condensates

    Depositor

    Michael Rosen

    Article

    Peeples et al Nat Chem Biol. 2021 Jun;17(6):693-702. d

    Insert

    ABL1 (ABL1 Human)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    tac

    Available since

    Oct. 14, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. ABI_cPPVp

    Plasmid

    #119212

    Purpose

    Expresses C-terminus of plum pox virus protease (PPVp) fused to the ABI-abscisic acid responsive protein domain

    Depositor

    Roman Jerala

    Article

    Fink et al Nat Chem Biol. 2019 Feb;15(2):115-122. d

    Insert

    split cPPVp in fusion with ABI
    

    Use

    Tags

    myc

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    April 1, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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