We narrowed to 12,203 results for: cel.2

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pEMS2230

Plasmid

#158420

Purpose

AAV plasmid with Ple253 (PITX3 MiniPromoter) driving expression of EmGFP. Contains WPRE.

Depositor

Elizabeth Simpson

Article

Korecki et al Gene Ther. 2021 Feb 2. pii: 10.1038/s414

Insert

Ple253 (PITX3 )

Use

AAV

Promoter

Ple253

Available Since

Feb. 4, 2021

Availability

Academic Institutions and Nonprofits only

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Lenti_CRISPRi_sgAR1

Plasmid

#167000

Purpose

Lentiviral expression of sgRNA targeted to the androgen receptor (AR) promoter for CRISPRi knockdown

Depositor

Gian-Paolo Dotto

Article

Ma et al J Exp Med. 2021 Feb 1;218(2). pii: 21150

Insert

AR Promoter sgRNA 1 (AR Human)

Use

CRISPR and Lentiviral

Promoter

U6

Available Since

April 23, 2021

Availability

Academic Institutions and Nonprofits only

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RRL.sin.cPPT.SFFV/Ace2.WPRE (MT136)

Plasmid

#145842

Purpose

Lentiviral vector genome to ectopically express human Ace2

Depositor

Caroline Goujon

Article

Rebendenne et al J Virol. 2021 Jan 29. pii: JVI.02415-20.

Insert

human Ace2 (ACE2 Human)

Use

Lentiviral

Expression

Mammalian

Promoter

SFFV

Available Since

May 13, 2020

Availability

Academic Institutions and Nonprofits only

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pHAGE N' EGFP NAP1 FL IRES Blast

Plasmid

#179100

Purpose

Expression of full length NAP1 cDNA

Depositor

Alicia Pickrell

Article

Paul et al J Cell Biol. 2024 Feb 5;223(2):e20230308

Insert

Nak associated protein 1 (AZI2 Human)

Use

Lentiviral

Tags

EGFP

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 31, 2023

Availability

Academic Institutions and Nonprofits only

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Lenti_CRISPRi_sgAR3

Plasmid

#167002

Purpose

Lentiviral expression of sgRNA targeted to the androgen receptor (AR) promoter for CRISPRi knockdown

Depositor

Gian-Paolo Dotto

Article

Ma et al J Exp Med. 2021 Feb 1;218(2). pii: 21150

Insert

AR Promoter sgRNA 3 (AR Human)

Use

CRISPR and Lentiviral

Promoter

U6

Available Since

April 23, 2021

Availability

Academic Institutions and Nonprofits only

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ACTB-(A)2x

Plasmid

#112056

Purpose

ACTB mRNA tagged with 2 copies of Riboglow (variant A)

Depositor

Amy Palmer

Article

Braselmann et al Nat Chem Biol. 2018 Jul 30. pii: 10.1038

Insert

ACTB (ACTB Human)

Tags

Riboglow RNA tag

Expression

Mammalian

Mutation

2 copies of Riboglow tag (variant A) after stop c…

Promoter

CMV

Available Since

June 29, 2018

Availability

Academic Institutions and Nonprofits only

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HOXB6 (human) FLAG pMSCV

Plasmid

#8534

Depositor

Corey Largman

Article

Shen et al Proc Natl Acad Sci U S A 1989 Nov;86(21)

Insert

HOXB6 (HOXB6 Human)

Use

Retroviral

Tags

FLAG and IRES-GFP

Expression

Mammalian

Mutation

contains improved ribosomal binding site and FLAG…

Available Since

May 30, 2006

Availability

Academic Institutions and Nonprofits only
pJC-elav1.8kb-TALE1-VP64-P10

Plasmid

#104609

Purpose

Expresses TALE1 under the control of a 1.8kb elav enhancer element

Depositor

Tudor Fulga

Article

Toegel et al Nat Commun. 2017 Nov 21;8(1):1663. doi:

Insert

1.8kb elav enhancer element (elav Fly)

Use

TALEN

Expression

Insect

Promoter

hsp70

Available Since

Jan. 29, 2018

Availability

Academic Institutions and Nonprofits only

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pEGFP Kind2 S159/181/666A

Plasmid

#105310

Purpose

To study altered downstream functions of phospholylation-site null kindlin2

Depositor

Kenneth Yamada

Article

Artym et al J Cell Biol. 2015 Feb 2;208(3):331-50. d

Insert

Kindlin2 (FERMT2 Human)

Tags

EGFP

Expression

Mammalian

Mutation

S159/181/666A

Available Since

Feb. 22, 2018

Availability

Academic Institutions and Nonprofits only

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pGEX Grap2-SH2

Plasmid

#46435

Depositor

Bruce Mayer

Article

Machida et al Mol Cell. 2007 Jun 22;26(6):899-915.

Insert

Grap2 (GRAP2 Human)

Tags

GST and PreScission

Expression

Bacterial

Mutation

contains amino acids 52-159

Promoter

Tac

Available Since

July 12, 2013

Availability

Academic Institutions and Nonprofits only

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pGEX-4T1_GST-GBRPL2

Plasmid

#223730

Purpose

Expression of recombinant protein for purification

Depositor

Sascha Martens

Article

Adriaenssens et al Nat Cell Biol. 2025 Jul 25. doi: 10.1038

Insert

GABARAPL2 (GABARAPL2 Human)

Tags

GST

Expression

Bacterial

Mutation

aa 2-116 only, with Glycine exposed

Available Since

March 25, 2025

Availability

Academic Institutions and Nonprofits only

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PGK_ACE2-ECD_Notch-Gal4VP64 (pZYW037)

Plasmid

#194226

Purpose

Lentiviral expression vector expressing ACE2-ECD SynNotch and mCherry transduction marker

Depositor

Hana El-Samad

Article

Weinberg et al bioRxiv 2021.04.20.440678

Insert

PGK-ACE2-SynNotch-T2A-mCherry (ACE2 Synthetic)

Use

Lentiviral

Promoter

PGK

Available Since

Sept. 10, 2024

Availability

Academic Institutions and Nonprofits only

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pMSCV-IRES-GFP/CALR ins5 YD/FL-FLAG

Plasmid

#214706

Purpose

Mammalian expression of human CALR ins5 YD/FL-FLAG

Depositor

Norio Komatsu

Article

Masubuchi et al Leukemia. 2020 Feb;34(2):499-509. doi: 1

Insert

CALR ins5 YD/FL-FLAG (CALR Human)

Use

Retroviral

Tags

FLAG

Promoter

MSCV

Available Since

April 10, 2024

Availability

Academic Institutions and Nonprofits only

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pCDNA3-SREBP-1-GFP (A317-318; A341; A1171)

Plasmid

#204199

Purpose

Mammalian expression of SREBP-1 (isoform 1)

Depositor

Allan Weissman

Article

Yang et al J Biol Chem. 2023 Oct 12;299(12):105346.

Insert

SREBP-1 (SREBF1 Human)

Tags

3xHA, eGFP and T7x2

Expression

Mammalian

Mutation

Gly to Ala 317,318,341,1171

Available Since

April 8, 2024

Availability

Academic Institutions and Nonprofits only

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pCMV_SEPT9_i1 S22A S23A-msfGFP

Plasmid

#180338

Purpose

mammalian expression of human SEPT9_i1 S22A S23A fused to monomeric superfolder GFP

Depositor

Manos Mavrakis

Article

Kuzmic et al J Cell Sci. 2021 Dec 2. pii: 273604. doi

Insert

SEPTIN9_v1 (SEPTIN9 Human)

Tags

msfGFP

Expression

Mammalian

Mutation

SEPT9_i1 S22A S23A

Promoter

CMV

Available Since

March 29, 2022

Availability

Academic Institutions and Nonprofits only

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pCMV_SEPT9_i1 R10A R15A-msfGFP

Plasmid

#180336

Purpose

mammalian expression of human SEPT9_i1 R10A R15A fused to monomeric superfolder GFP

Depositor

Manos Mavrakis

Article

Kuzmic et al J Cell Sci. 2021 Dec 2. pii: 273604. doi

Insert

SEPTIN9_v1 (SEPTIN9 Human)

Tags

msfGFP

Expression

Mammalian

Mutation

SEPT9_i1 R10A R15A

Promoter

CMV

Available Since

March 29, 2022

Availability

Academic Institutions and Nonprofits only

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pCMV_SEPT9_i1 S12A S13A-msfGFP

Plasmid

#180337

Purpose

mammalian expression of human SEPT9_i1 S12A S13A fused to monomeric superfolder GFP

Depositor

Manos Mavrakis

Article

Kuzmic et al J Cell Sci. 2021 Dec 2. pii: 273604. doi

Insert

SEPTIN9_v1 (SEPTIN9 Human)

Tags

msfGFP

Expression

Mammalian

Mutation

SEPT9_i1 S12A S13A

Promoter

CMV

Available Since

March 29, 2022

Availability

Academic Institutions and Nonprofits only

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Lenti_CRISPRi_sgAR10

Plasmid

#167003

Purpose

Lentiviral expression of sgRNA targeted to the androgen receptor (AR) promoter for CRISPRi knockdown

Depositor

Gian-Paolo Dotto

Article

Ma et al J Exp Med. 2021 Feb 1;218(2). pii: 21150

Insert

AR Promoter sgRNA 10 (AR Human)

Use

CRISPR and Lentiviral

Promoter

U6

Available Since

April 23, 2021

Availability

Academic Institutions and Nonprofits only

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DHC1-mIAA17 Hygro

Plasmid

#140544

Purpose

DHC1 tagging with mIAA7

Depositor

Masato Kanemaki

Article

Yesbolatova et al Nat Commun. 2020 Nov 11;11(1):5701. doi:

Insert

DHC1 (DYNC1H1 Human)

Use

CRISPR

Expression

Mammalian

Available Since

Nov. 12, 2020

Availability

Academic Institutions and Nonprofits only

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pCDNA5-LAP-MPS1-dTPR

Plasmid

#114048

Purpose

Expression of exogenous LAP tagged MPS1 delta TPR (TPR is deleted)

Depositor

Geert Kops

Article

Nijenhuis et al J Cell Biol. 2013 Apr 15;201(2):217-31.

Insert

MPS1-dTPR (TTK Human)

Expression

Mammalian

Mutation

delta TPR (deletion of TPR domain 63-191)

Available Since

Nov. 7, 2018

Availability

Academic Institutions and Nonprofits only

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pCDNA5-LAP-MPS1-d60

Plasmid

#114046

Purpose

Expression of exogenous LAP tagged MPS1 delta 60 (NTE is deleted)

Depositor

Geert Kops

Article

Nijenhuis et al J Cell Biol. 2013 Apr 15;201(2):217-31.

Insert

MPS1-d60 (TTK Human)

Expression

Mammalian

Mutation

delta 60 (deletion of NTE domain)

Available Since

Sept. 11, 2018

Availability

Academic Institutions and Nonprofits only

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pEGFP Kind2 S159/181/666E

Plasmid

#105314

Purpose

To study altered downstream functions of phospholylation-site mimetic kindlin2

Depositor

Kenneth Yamada

Article

Artym et al J Cell Biol. 2015 Feb 2;208(3):331-50. d

Insert

Kindlin2 (FERMT2 Human)

Tags

EGFP

Expression

Mammalian

Mutation

S159/181/666E

Available Since

Feb. 22, 2018

Availability

Academic Institutions and Nonprofits only

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pSH-Csy4-T2A-SpRFN

Plasmid

#85754

Purpose

Expresses S. pyogenes FokI-dCas9-NLS with a 25 amino acid linker (GGGGS)5 fusion. Also expresses Csy4 for cleavage of multiplexed gRNA transcripts. Derived from pSQT1601 (Addgene #53369).

Depositor

Lawrence Stanton

Article

Havlicek et al Mol Ther. 2017 Feb 1;25(2):342-355. doi:

Insert

Csy4-T2A-FokI-dCas9

Use

CRISPR

Tags

Csy4 and FokI fusion via 25 amino acid (GGGGS)5 l…

Expression

Mammalian

Mutation

D10A, H840A

Promoter

CAG

Available Since

Jan. 20, 2017

Availability

Academic Institutions and Nonprofits only

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pMK297 (DHC1-mAID Hygro)

Plasmid

#140542

Purpose

DHC1 tagging with mAID

Depositor

Masato Kanemaki

Article

Yesbolatova et al Nat Commun. 2020 Nov 11;11(1):5701. doi:

Insert

DHC1 (DYNC1H1 Human)

Use

CRISPR

Expression

Mammalian

Available Since

Nov. 12, 2020

Availability

Academic Institutions and Nonprofits only

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pMK296 (DHC1-mAID Neo)

Plasmid

#140541

Purpose

DHC1 tagging with mAID

Depositor

Masato Kanemaki

Article

Yesbolatova et al Nat Commun. 2020 Nov 11;11(1):5701. doi:

Insert

DHC1 (DYNC1H1 Human)

Use

CRISPR

Expression

Mammalian

Available Since

Nov. 12, 2020

Availability

Academic Institutions and Nonprofits only

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pEVOL-pAzF[TAG]

Plasmid

#164579

Purpose

Machinery plasmid containing two copies of the Mj pCNFRS and cognate tRNA for incorporation of pAzF, pCNF, or pENF at TAG codons

Depositor

Ryan Mehl

Article

Bednar et al ACS Chem Biol. 2021 Nov 19;16(11):2612-2

Inserts

Mj pCNFRS[TAG] (aaRS1)
Mj pCNFRS[TAG] (aaRS2)
Mj tRNA[TAG]

Expression

Bacterial

Mutation

Y32L L65V F108W Q109M D158G I159P

Promoter

araBAD, glnS, and proK

Available Since

May 11, 2023

Availability

Academic Institutions and Nonprofits only

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tetO-HAND2

Plasmid

#170690

Purpose

doxycycline-inducible overexpression of HAND2

Depositor

Paul Burridge

Article

Romero-Tejeda et al J Mol Cell Cardiol. 2023 Jul 6:S0022-282

Insert

HAND2 (HAND2 Human)

Use

Lentiviral; Doxycycline inducible

Expression

Mammalian

Promoter

TRE promoter, Tet-ON

Available Since

July 12, 2023

Availability

Academic Institutions and Nonprofits only

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pEMS2181

Plasmid

#158421

Purpose

AAV plasmid with Ple32 (CLDN5 MiniPromoter) driving expression of EmGFP. Contains WPRE.

Depositor

Elizabeth Simpson

Article

Korecki et al Gene Ther. 2021 Feb 2. pii: 10.1038/s414

Insert

Ple32 (CLDN5 Human)

Use

AAV

Promoter

Ple32

Available Since

Feb. 4, 2021

Availability

Academic Institutions and Nonprofits only

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CRY2high-mCherry-Raf1

Plasmid

#104064

Purpose

Expresses fusion of CRY2high mutant (CRY2PHR E490R) with mCherry and Raf1

Depositor

Bianxiao Cui

Article

Duan et al Nat Commun. 2017 Sep 15;8(1):547. doi: 1

Insert

CRY2high-mCherry-Raf1 (CRY2, RAF1 Human, Mustard Weed)

Expression

Mammalian

Mutation

CRY2PHR E490R

Promoter

CMV

Available Since

Dec. 19, 2017

Availability

Academic Institutions and Nonprofits only

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RRL.sin.cPPT.SFFV/Ace2.IRES-neo.WPRE (MT129)

Plasmid

#145840

Purpose

Lentiviral vector genome to ectopically express human Ace2

Depositor

Caroline Goujon

Article

Rebendenne et al J Virol. 2021 Jan 29. pii: JVI.02415-20.

Insert

human Ace2 (ACE2 Human)

Use

Lentiviral

Expression

Mammalian

Promoter

SFFV

Available Since

May 13, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge