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We narrowed to 18,870 results for: IRE

Showing: 8761 - 8800 of 18870 results

  3. pGEX6P1‐Afu‐rnhBΔPIP

    Plasmid
    #108696
    Purpose
    For expression in E. coli, and affinity purification of N-terminally GST-tagged Archaeoglobus fulgidus RNase HII with C-terminal PIP box deletion
    Depositor
    Andrew Jackson, Martin Reijns
    Article
    Bubeck et al Nucleic Acids Res. 2011 May;39(9):3652-6
    Insert
    rnhB
    Use
    Tags
    GST
    Expression
    Bacterial
    Mutation
    C-terminal truncation (7aa) removing PIP box
    Promoter
    tac
    Available since
    April 24, 2018
    Availability
    Academic Institutions and Nonprofits only
    108696-plasmid-map-sequence-id-208999

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  5. pNB784

    Plasmid
    #60163
    Purpose
    Firefly luciferase (Promega) yeast codon-optimized yellow fluorescent protein (yEVenus) fusion driven by SIC1 promoter.
    Depositor
    Nicolas Buchler
    Article
    Mazo-Vargas et al Mol Biol Cell. 2014 Sep 17. pii: mbc.E14
    Insert
    FLuc-yEVenus
    Use
    Tags
    Expression
    Yeast
    Mutation
    FLuc has A4V & S504G (no functional effect)
    Promoter
    SIC1pr
    Available since
    March 25, 2015
    Availability
    Academic Institutions and Nonprofits only
    60163-plasmid-map-sequence-id-96372

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  6. pJH2827

    Plasmid
    #179542
    Purpose
    Pnmr-1::tomm20::miniSOG-SL2::RFP unc-54 3' UTR C.elegans AVA/E/D premotor IN and others expression of miniSOG-SL2 RFP
    Depositor
    Mei Zhen
    Article
    Gao et al Elife. 2018 Jan 23;7. pii: 29915. doi: 1
    Insert
    tomm20::miniSOG (tomm-20 Nematode)
    Use
    Tags
    wCherry
    Expression
    Worm
    Mutation
    Promoter
    Pnmr-1
    Available since
    March 1, 2022
    Availability
    Academic Institutions and Nonprofits only
    179542-plasmid-map-sequence-id-355649

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  11. pTorPE-Y-GECO1

    Plasmid
    #55765
    Purpose
    Encoded a yellow fluorescent calcium ion indicator with inverted fluorescence response for expression in bacterial cells
    Depositor
    Robert Campbell
    Article
    Zhao et al Integr Biol (Camb). 2014 May 19.
    Insert
    Y-GECO1.0
    Use
    Lab constructed
    Tags
    6x His tag
    Expression
    Bacterial
    Mutation
    Promoter
    pBAD
    Available since
    July 22, 2014
    Availability
    Academic Institutions and Nonprofits only
    55765-plasmid-map-sequence-id-89444

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  18. pUE1-TSP-ORF68 K395A/K396A

    Plasmid
    #162656
    Purpose
    Expresses ORF68 K395A/K396A in mammalian cells with an N-terminal TwinStrep tag and HRV 3C protease cleavage site
    Depositor
    Britt Glaunsinger
    Article
    Didychuk et al Elife. 2021 Feb 8;10. pii: 62261. doi: 1
    Insert
    ORF68
    Use
    Tags
    N-terminal 2xStrep-Tag II and HRV 3C site
    Expression
    Mammalian
    Mutation
    Amino acids K395 and K396 mutated to alanine
    Promoter
    CMV
    Available since
    Feb. 17, 2021
    Availability
    Academic Institutions and Nonprofits only
    162656-plasmid-map-sequence-id-320674

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  21. pWL502-PAM

    Plasmid
    #174382
    Purpose
    Plasmids bearing protospacer sequences with functional PAM efficiently triggered CRISPR-mediated defense in cells with corresponding spacer sequence; article demonstrates use in Haloferax mediterranei
    Depositor
    Jing Han
    Article
    Lin et al Commun Biol. 2021 Aug 25;4(1):1007. doi:
    Insert
    PAM-spacer
    Use
    CRISPR; Archaeal expression
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    Nov. 11, 2021
    Availability
    Academic Institutions and Nonprofits only
    174382-plasmid-map-sequence-id-344136

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  24. pJH3409

    Plasmid
    #179574
    Purpose
    Pceh-12::tomm20::miniSOG-SL2::RFP unc-54 3' UTR C.elegans VB-MN -specific expression of miniSOG-SL2 RFP
    Depositor
    Mei Zhen
    Article
    Gao et al Elife. 2018 Jan 23;7. pii: 29915. doi: 1
    Insert
    tomm20::miniSOG (tomm-20 Nematode)
    Use
    Tags
    wCherry
    Expression
    Worm
    Mutation
    Promoter
    Pceh-12
    Available since
    March 1, 2022
    Availability
    Academic Institutions and Nonprofits only
    179574-plasmid-map-sequence-id-355658

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  25. pJK2067

    Plasmid
    #171845
    Purpose
    C. elegans mex-5 promoter (germline) driven dual fluorescent reporter for boxB tethering (eGFP) with boxB negative control (mCherry)
    Depositor
    Judith Kimble
    Article
    Aoki et al Nat Commun. 2021 Feb 12;12(1):996. doi:
    Insert
    his-58, eGFP, mCherry
    Use
    Tags
    N-terminal Tag OLLAS, V5 and C-terminal Tag PEST…
    Expression
    Worm
    Mutation
    boxB wild type and hairpin mutant
    Promoter
    mex-5 promoter
    Available since
    July 8, 2021
    Availability
    Academic Institutions and Nonprofits only
    171845-plasmid-map-sequence-id-337181

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  29. pET21-pelB-LaG-2

    Plasmid
    #172739
    Purpose
    Bacterial expression of HIS-tagged anti-GFP nanobody LaG-2; HIS tag preceded by HRV 3C/PreScission cleavage site.
    Depositor
    Michael Rout
    Article
    Fridy et al Nat Methods. 2014 Dec;11(12):1253-60. do
    Insert
    LaG-2
    Use
    Tags
    6xHIS and pelB leader for periplasmic secretion
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Available since
    Aug. 18, 2021
    Availability
    Academic Institutions and Nonprofits only
    172739-plasmid-map-sequence-id-340192

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  33. pT7-AsCas12a_crRNA-site3 (RTW549)

    Plasmid
    #160138
    Purpose
    T7 promoter expression plasmid for in vitro transcription of AsCas12a crRNA with spacer #3
    Depositor
    Benjamin Kleinstiver, Keith Joung
    Article
    Walton et al Nat Protoc. 2021 Feb 5. pii: 10.1038/s41
    Insert
    AsCas12a crRNA with spacer #3 (spacer=CTGATGGTCCATGTCTGTTACTC)
    Use
    In vitro transcription of sgrna from t7 promoter
    Tags
    Expression
    Mutation
    Promoter
    T7
    Available since
    Feb. 8, 2021
    Availability
    Academic Institutions and Nonprofits only
    160138-plasmid-map-sequence-id-333508

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Showing: 8761 - 8800 of 18870 results