We narrowed to 79,981 results for: TRI

1. pGREAT2

   Plasmid

   #170890

   Purpose

   GoldenBraid Double Transcriptional Unit - p35SoTMV_E-Luc_NOSt+pNOS_Red-F_NOSt

   Depositor

   Markita Landry

   Article

   Gonzalez-Grandio et al ACS Synth Biol. 2021 Sep 14. doi: 10.102

   Insert

   E-Luc
   

   Use

   Luciferase and Synthetic Biology

   Tags

   Expression

   Plant

   Mutation

   c.375C>T silent mutation to remove BbsI site (…

   Promoter

   p35SoTMV

   Available since

   Oct. 4, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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2. GST-Hsp90 C(626-732)

   Plasmid

   #22483

   Depositor

   William Sessa

   Article

   Fontana et al Circ Res. 2002 May 3. 90(8):866-73.

   Insert

   Hsp90alpha C domain (HSP90AA2P Human)
   

   Use

   Tags

   GST

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   April 5, 2010

   Availability

   Academic Institutions and Nonprofits only

   

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3. pMXS-IRES-Blast coUGP2

   Plasmid

   #211531

   Purpose

   Expresses codon optimized UGP2

   Depositor

   Richard Possemato

   Article

   Nam et al Nat Metab. 2024 Dec;6(12):2338-2353. doi

   Insert

   UGP2 (UGP2 Human)
   

   Use

   Retroviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Jan. 31, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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4. pGREAT1

   Plasmid

   #170889

   Purpose

   GoldenBraid Double Transcriptional Unit - pNOS_E-Luc_NOSt+pNOS_Red-F_NOSt

   Depositor

   Markita Landry

   Article

   Gonzalez-Grandio et al ACS Synth Biol. 2021 Sep 14. doi: 10.102

   Insert

   E-Luc
   

   Use

   Luciferase and Synthetic Biology

   Tags

   Expression

   Plant

   Mutation

   c.375C>T silent mutation to remove BbsI site (…

   Promoter

   pNOS

   Available since

   Aug. 11, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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5. pCA24N-ligase-cTF

   Plasmid

   #87743

   Purpose

   IPTG-inducible expression of ligase-cTF fusion for protein purification

   Depositor

   Wayne Patrick

   Article

   Wilson et al Protein Eng Des Sel. 2013 Jul;26(7):471-

   Insert

   T4 DNA ligase (30 )
   

   Use

   Tags

   6xHis and cTF (chimeric transcription factor base…

   Expression

   Bacterial

   Mutation

   Promoter

   T5-lac

   Available since

   March 21, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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6. pTEV5_ RpMatB_2

   Plasmid

   #78206

   Purpose

   Expression of double-cysteine variant of Malonyl-coenzyme A synthetase, RpMatB, as scaffold for ATP biosensor - tighter binding

   Depositor

   Martin Webb

   Article

   Vancraenenbroeck et al PLoS One. 2017 Jun 21;12(6):e0179547. do

   Insert

   matB (His6 C106A S170A R286C Q457C K488A)
   

   Use

   Tags

   His6

   Expression

   Bacterial

   Mutation

   C106A S170A R286C Q457C K488A

   Promoter

   T7

   Available since

   July 11, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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7. pGEX-6P-1-HsRCK_E

   Plasmid

   #146210

   Purpose

   Bacterial Expression of HsRck

   Depositor

   Elisa Izaurralde

   Article

   Tritschler et al Mol Cell. 2009 Mar 13. 33(5):661-8.

   Insert

   HsRck (DDX6 Human)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   March 16, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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8. pSB-CMV-MCS-puro PM-mKate2-P2A-GEM-LTB4mut

   Plasmid

   #202644

   Purpose

   Expresses the LTB4-insensitive control sensor GEM-LTB4mut along with the plasma membrane targeted mKate2 tag in mammalian cells

   Depositor

   Balázs Enyedi

   Article

   Tamas et al Nat Commun. 2023 Aug 1;14(1):4610. doi:

   Insert

   PM-mKate2-P2A-GEM-LTB4mut
   

   Use

   Tags

   PM-mKate2

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Aug. 8, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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9. pCAG_EGFP-RandE3

   Plasmid

   #79870

   Purpose

   Expresses under the CAG promoter RandE3, a synthetic E3 ligase with random residues in its binding domain; control construct for GFE3.

   Depositor

   Don Arnold

   Article

   Gross et al Nat Methods. 2016 Jun 6. doi: 10.1038/nm

   Insert

   GFP-RandE3
   

   Use

   Synthetic Biology

   Tags

   GFP and HA

   Expression

   Mammalian

   Mutation

   Promoter

   Beta-actin

   Available since

   Aug. 8, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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10. pMuLE_ENTR_CBF-GLuc_L3-L2

    Plasmid

    #113868

    Purpose

    Single pathway reporter; notch promoter-driven expression of secreted Gaussia luciferase; MultiSite Gateway entry vector.

    Depositor

    Manfred Ogris

    Article

    Maier et al PLoS One. 2019 Dec 20;14(12):e0226570. d

    Inserts

    CBF (notch dependent promoter)
    GLuc
    

    Use

    Mule gateway entry vector

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 29, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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11. gRNA-leu-HYB

    Plasmid

    #64332

    Purpose

    leu2 deletion gRNA cassette carried by pRS42H

    Depositor

    Yong-Su Jin

    Article

    Zhang et al Appl Environ Microbiol. 2014 Dec;80(24):

    Insert

    gBlock product of leu2 deletion gRNA cassette
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    April 29, 2015

    Availability

    Academic Institutions and Nonprofits only

12. p5M-hPR-B

    Plasmid

    #89114

    Purpose

    mammalian expression of full-length human progesterone receptor-B in pCMV5 with 5 restriction sites deleted in polylinker

    Depositor

    Elizabeth Wilson

    Article

    Su et al J Biol Chem. 2012 Oct 5;287(41):34809-24

    Insert

    human progesterone receptor-B (PGR Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    5 restriction sites deleted in polylinker

    Promoter

    CMV

    Available since

    April 21, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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13. pEYFP-N1-eKR2

    Plasmid

    #115337

    Purpose

    Expresses enhanced sodium pump for light-driven extrusion of sodium; activation with green light; codon-optimized for human/mouse

    Depositor

    Peter Hegemann

    Article

    Grimm et al Sci Rep. 2018 Jun 18;8(1):9316. doi: 10.

    Insert

    eKR2
    

    Use

    Tags

    EYFP

    Expression

    Mammalian

    Mutation

    none

    Promoter

    CMV

    Available since

    Sept. 13, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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14. Twitch-4 pcDNA3

    Plasmid

    #49533

    Purpose

    Fluorescent reporter for calcium signaling

    Depositor

    Oliver Griesbeck

    Article

    Thestrup et al Nat Methods. 2014 Jan 5. doi: 10.1038/nm

    Insert

    Twitch-4
    

    Use

    Tags

    ECFP and cpCit174

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Jan. 14, 2014

    Availability

    Academic Institutions and Nonprofits only

15. Twitch-1 pcDNA3

    Plasmid

    #49812

    Purpose

    Fluorescent reporter for calcium signaling

    Depositor

    Oliver Griesbeck

    Article

    Thestrup et al Nat Methods. 2014 Jan 5. doi: 10.1038/nm

    Insert

    Twitch-1
    

    Use

    Tags

    ECFP and cpCit174

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Jan. 16, 2014

    Availability

    Academic Institutions and Nonprofits only

16. pX330-UGCG-KO

    Plasmid

    #80010

    Purpose

    gRNA to knock out expression of UGCG gene. The product of this gene catalyzes the first step in synthesis of glycosphingolipids.

    Depositor

    Sriram Neelamegham

    Article

    Stolfa et al Sci Rep. 2016 Jul 26;6:30392. doi: 10.10

    Insert

    UGCG (UGCG Human)
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    July 25, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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17. pLenti-CMV-GFP-2A-G(N2c)-WPRE

    Plasmid

    #194352

    Purpose

    Lentivirus expressing eGFP and Rabies Glycoprotein (G) from CVS-N2c strain

    Depositor

    Marco Tripodi

    Article

    Lee et al Nat Methods. 2023 Apr;20(4):580-589. doi

    Inserts

    Enhanced Green Fluorescent Protein (eGFP)
    Rabies CVS-N2c glycoprotein (G)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Aug. 30, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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18. pMuLE_ENTR_PTCH1-mTurquoise2_R4-R3

    Plasmid

    #113864

    Purpose

    Single pathway reporter; hedgehog promoter-driven expression of mTurquoise2; MultiSite Gateway entry vector.

    Depositor

    Manfred Ogris

    Article

    Maier et al PLoS One. 2019 Dec 20;14(12):e0226570. d

    Inserts

    PTCH1 (hedgehog dependent promoter)
    mTurquoise2
    

    Use

    Mule gateway entry vector

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Jan. 22, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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19. pAAV-E1.1-RFP

    Plasmid

    #22928

    Depositor

    Edward Callaway

    Article

    Nathanson et al Front Neural Circuits. 2009 Nov 9;3:19.

    Insert

    E1.1 binding site from Scardigli et al., 2003 with minimal CMV
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    May 25, 2010

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pUC19-U6-LwaCas13acrRNA-BsmBIcassette (LTH151)

    Plasmid

    #171129

    Purpose

    LwaCas13a crRNA entry vector for pU6 expression (need to clone in spacer into BsmBI sites)

    Depositor

    Benjamin Kleinstiver

    Article

    Johnson et al Mol Cell. 2022 Jun 2;82(11):2161-2166.e3

    Insert

    U6 promoter entry plasmid for LwaCas13a crRNA cloning
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    July 6, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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