We narrowed to 5,898 results for: dna plasmids

1. PB ID1-iCreERT2

   Plasmid

   #139280

   Purpose

   Human ID1-iCreERT2 transgene piggyBac transposon

   Depositor

   Mario Capecchi

   Insert

   ID1 (ID1 Human)
   

   Use

   Cre/Lox

   Expression

   Mammalian

   Mutation

   Replaced the ID1 protein coding sequence in the t…

   Available Since

   May 13, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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2. pCJH027_SC101_pLAC_LbCas12a_Kan

   Plasmid

   #183074

   Purpose

   Wild-type LbCas12a (Chen et al., 2018) in a plasmid containing a SC101 origin and lac promoter for in vivo interference assay.

   Depositor

   Jennifer Doudna

   Article

   Huang et al Mol Cell. 2022 Jun 2;82(11):2148-2160.e4

   Insert

   LbCas12a
   

   Expression

   Bacterial

   Mutation

   Wild-type

   Available Since

   June 2, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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3. pCJH065_proD_Cas12c_DualRNA-GFP+HDVrz_CAM

   Plasmid

   #183077

   Purpose

   Plasmid expressing Cas12c tracrRNA and GFP-targeting crRNA separately, with the crRNA ending with a HDV ribozyme sequence.

   Depositor

   Jennifer Doudna

   Article

   Huang et al Mol Cell. 2022 Jun 2;82(11):2148-2160.e4

   Insert

   Cas12c tracrRNA and crRNA ending with a HDV ribozyme
   

   Use

   CRISPR

   Expression

   Bacterial

   Available Since

   June 2, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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4. pCJH026_proD_Cas12c-multiplexing_CAM

   Plasmid

   #183073

   Purpose

   Plasmid expressing a Cas12c sgRNA targeting GFP and another sgRNA targeting RFP in one transcript for testing multiplexed repression

   Depositor

   Jennifer Doudna

   Article

   Huang et al Mol Cell. 2022 Jun 2;82(11):2148-2160.e4

   Insert

   Expressing two Cas12c sgRNAs (targeting GFP or RFP) in one transcript
   

   Use

   CRISPR

   Expression

   Bacterial

   Available Since

   June 2, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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5. pLenti-Tet-coBxb1-2A-BFP_IRES-iCasp9-2A-Blast_rtTA3

   Plasmid

   #171588

   Purpose

   Lentivector encoding a DNA recombinase landing pad with Bxb1 integrase and iCasp9 negative selection behind a Tet-inducible promoter

   Depositor

   Kenneth Matreyek

   Article

   Shukla et al PLoS Pathog. 2021 Jul 16;17(7):e1009715.

   Inserts

   Bxb1 integrase
   iCasp9
   BFP
   rtTA3G
   

   Use

   Lentiviral and Synthetic Biology

   Expression

   Mammalian

   Available Since

   July 21, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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6. pYPQ202

   Plasmid

   #86198

   Purpose

   Multisite Gateway T-DNA entry plasmid (attR1 & attR2); AtUBQ10 promoter and Hygromiycin resistance marker

   Depositor

   Yiping Qi

   Article

   Tang et al Nat Plants. 2017 Feb 17;3:17018. doi: 10

   Type

   Empty backbone

   Use

   Gateway compatible attr1-attr2 destination vector

   Expression

   Plant

   Promoter

   Arabidopsis ubiquitin 10 (AtUbi10)

   Available Since

   May 2, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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7. pCA11-pMa-sgTS3-17xMS2V5-sgTS3

   Plasmid

   #199442

   Purpose

   CRISPR donor plasmid to insert 17XMS2V5 into the ribosomal DNA region in dependent of homology arms

   Depositor

   Baohui Chen

   Article

   Fu et al J Cell Biol. 2023 Jan 2;222(1):e20220211

   Insert

   HITI donor template
   

   Use

   CRISPR

   Available Since

   May 4, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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8. LLP783_SpyCatcher-DNMT3A-CO

   Plasmid

   #211776

   Purpose

   SpyTag counterpart binding domain, SpyCatcher, fused to DNA methyltransferase DNMT3A, with GFP selection

   Depositor

   Ryan Lister

   Article

   Swain et al Nucleic Acids Res. 2023 Nov 24:gkad1108.

   Insert

   Traptavudin-DNMT3A
   

   Tags

   3xV5

   Expression

   Mammalian

   Mutation

   Last 300 bp codon-optimised (CO) to detect DNMT3A…

   Promoter

   pEF1a and pSV40

   Available Since

   Dec. 15, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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9. pP2A_NLS_sfGFP

   Plasmid

   #63709

   Purpose

   Expressing P2A_NLS_sfGFP with homologous arm of Nanog.

   Depositor

   Stanley Qi

   Article

   Yu et al Cell Stem Cell. 2015 Feb 5;16(2):142-7.

   Insert

   Nanog homologous arm, p2A, NLS, sfGFP, Nanog homologous arm
   

   Use

   Just a donor dna with nanog homologous arm for kn…

   Tags

   sfGFP

   Available Since

   April 27, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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10. LLP784_SnoopCatcher-DNMT3A-CO

    Plasmid

    #211777

    Purpose

    SnoopTag counterpart binding domain, SnoopCatcher, fused to DNA methyltransferase DNMT3A, with GFP selection

    Depositor

    Ryan Lister

    Article

    Swain et al Nucleic Acids Res. 2023 Nov 24:gkad1108.

    Insert

    Spy-EZH2
    

    Tags

    3xFLAG

    Expression

    Mammalian

    Mutation

    Last 300 bp codon-optimised (CO) to detect EZH2 e…

    Promoter

    pEF1a and pSV40

    Available Since

    Dec. 15, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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11. LLP789_αGCN4-DNMT3A-CO

    Plasmid

    #211783

    Purpose

    SunTag counterpart binding domain, aGCN4, fused to DNA methyltransferase DNMT3A, with GFP selection

    Depositor

    Ryan Lister

    Article

    Swain et al Nucleic Acids Res. 2023 Nov 24:gkad1108.

    Insert

    aGCN4-DNMT3A
    

    Tags

    3xTy1

    Expression

    Mammalian

    Mutation

    Last 300 bp codon optimised (CO) to detect DNMT3A…

    Promoter

    pEF1a and pSV40

    Available Since

    Dec. 13, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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12. pRpr150-LUC

    Plasmid

    #85700

    Purpose

    luciferase reporter driven by a DNA damage responsive cis-regulatory sequence from the reaper locus, containing a p53 binding site (Rpr150 enhancer)

    Depositor

    Licio Collavin, Giannino Del Sal

    Article

    Mauri et al J Biol Chem. 2008 Jul 25. 283(30):20848-

    Insert

    RpR 150 enhancer and hsp70 minimal promoter
    

    Use

    Luciferase

    Tags

    luciferase

    Expression

    Mammalian

    Promoter

    RpR 150 enhancer and hsp70 minimal promoter

    Available Since

    March 21, 2017

    Availability

    Academic Institutions and Nonprofits only

13. LLP785_Traptavidin-DNMT3A-CO

    Plasmid

    #211778

    Purpose

    AviTag counterpart binding domain, Traptavidin, fused to DNA methyltransferase DNMT3A, with GFP selection

    Depositor

    Ryan Lister

    Article

    Swain et al Nucleic Acids Res. 2023 Nov 24:gkad1108.

    Insert

    Traptavidin-EZH2
    

    Tags

    3xV5

    Expression

    Mammalian

    Mutation

    Last 300 bp codon-optimised (CO) to detect EZH2 e…

    Promoter

    pEF1a and pSV40

    Available Since

    Dec. 15, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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14. Gal4-HSF4b WT

    Plasmid

    #118358

    Purpose

    This plasmid expresses amino-acids 200-493 of hHSF4b WT fused to the GAL-4 DNA binding domain.

    Depositor

    Lea Sistonen

    Article

    Hietakangas et al Proc Natl Acad Sci U S A. 2006 Jan 3;103

    Insert

    hHSF4b (HSF4 Human)
    

    Expression

    Mammalian

    Mutation

    WT

    Available Since

    Nov. 8, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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15. pJK193

    Plasmid

    #71834

    Purpose

    Cloned region contains Acetobacter aceti 1023 rpmE-radC(and beyond) region

    Depositor

    T. J. Kappock

    Article

    Constantine et al Biochemistry. 2006 Jul 11;45(27):8193-20

    Inserts

    N5-carboxyaminoimidazole ribonucleotide mutase
    N5-carboxyaminoimidazole ribonucleotide synthetase
    

    Use

    Phage lambda cloning vector with genomic dna inse…

    Mutation

    additional N-terminal methionine encoded by this …

    Available Since

    Jan. 5, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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16. TAL3467

    Plasmid

    #47186

    Depositor

    Keith Joung

    Article

    Sander et al Nat Biotechnol. 2011 Aug 5;29(8):697-8.

    Insert

    ZebrafishCommunity-inhibitorofDNAbinding3-right (id3 Zebrafish)
    

    Use

    T7

    Tags

    3X Flag and WT FOKI

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Aug. 13, 2013

    Availability

    Academic Institutions and Nonprofits only

17. TAL3466

    Plasmid

    #47185

    Depositor

    Keith Joung

    Article

    Sander et al Nat Biotechnol. 2011 Aug 5;29(8):697-8.

    Insert

    ZebrafishCommunity-inhibitorofDNAbinding3-left (id3 Zebrafish)
    

    Use

    T7

    Tags

    3X Flag and WT FOKI

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Aug. 13, 2013

    Availability

    Academic Institutions and Nonprofits only

18. pCasY1

    Plasmid

    #87687

    Purpose

    p15A plasmid expressing CasY1

    Depositor

    Jennifer Doudna

    Article

    Burstein et al Nature. 2016 Dec 22. doi: 10.1038/nature

    Insert

    CasY1
    

    Expression

    Bacterial

    Available Since

    April 27, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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19. pCX420

    Plasmid

    #229211

    Purpose

    Cytoplasmic-targeting Csm complex plasmid

    Depositor

    Jennifer Doudna

    Article

    Xia et al Nat Biotechnol. 2025 Feb 18. doi: 10.103

    Insert

    Csm1; Csm2; Csm3(RNase mut)-2xsfGFP; Csm4; Csm5; Cas6
    

    Expression

    Mammalian

    Available Since

    Feb. 18, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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20. Synaptotagmin-7 scFv [N275/14]

    Plasmid

    #211608

    Purpose

    Mammalian Expression Plasmid of Synaptotagmin-7 scFv. Derived from hybridoma N275/14.

    Depositor

    James Trimmer

    Article

    Mitchell et al Sci Rep. 2023 Sep 27;13(1):16200. doi: 1

    Insert

    Synaptotagmin-7 (Rattus norvegicus) recombinant mouse scFv. (Syt7 Mouse)
    

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Jan. 16, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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